BacMap

Definition	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence.
Accession	NC_008378
Length	870,021

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The map label for this gene is crt [H]

Identifier: 116248745

GI number: 116248745

Start: 71462

End: 72265

Strand: Reverse

Name: crt [H]

Synonym: pRL120069

Alternate gene names: 116248745

Gene position: 72265-71462 (Counterclockwise)

Preceding gene: 116248758

Following gene: 116248741

Centisome position: 8.31

GC content: 64.93

Gene sequence:

>804_bases
ATGGCCGACACTGTTTTGATCGACAGCCGCGACGGAATCGCCACGCTCACTCTCAATCGCCCGGAAAAGCTCAATGCGCT
GAATTACGCCTTGATAGACCGCTTTCTCGCGATCCTCGACGCCATCGAAACCGACAGTTCAATCCGGGCGATCATCCTCA
CCGGCGCAGGCGAGCGGGCGTTCTCGGCGGGCGGCGATATCTACGAATTCTCGGAAAGCGTGGCGCAGGGCGCCGATGTC
GCCATGCGCGATTTCGTCGCGCGCGGACAGCGCCTAACTGCGCGGCTGGAAGCCTTCCGCAAACCCGTCATCGCCGCCGT
CAACGGCCTTGCCTTTGGCGGCGGCTGCGAGATCACCGAGGCTGTGCCGCTCGCCATCGCCAGCGAATGCGCCCTGTTCG
CCAAGCCCGAGATCAATCTCGCAATGCCGCCGACCTTTGGCGGAACGCAGCGTTTGCCAAGGCTGGCCGGGCGCAAGCGC
GCCCTCGAATTGCTGTTGACGGGCGACGCATTCTCCCCGCAGCGCGCCCTCGAACTCGGCCTTGTCAACCAAGTTGTCCC
GCATGACGCTCTCATGCCGGCCGCCCACGACCTCGCGCGGCGCATTCGGCGTCATTCACCGCTGGCGGCGGCGAGCATTC
TGACGGCGGTGACGCGCGGCATCAACCAGAGCATTGCGGAGGGGCTGCTGATCGAAGGCGAACAATTCGCCCGGATGGCG
CCAACCGCCGATTTGCGCGAAGGGCTCGACGGGTGGATCGAGCGTCGCAAGCCGAACTATCCCGGCTCGTGGTCGCTCGA
TTGA

Upstream 100 bases:

>100_bases
AGGTGAGATCATGCCGTTATGTATACCGACTAGTGAATATACACAAGCATCGACCGGCGGCATACTTCTCCTGACGCAGC
ATACCGGAAAGGAAAACCGA

Downstream 100 bases:

>100_bases
TTGCCGCCAACACTGCCGAGAATGGCTCAGTCAGCGTAATCAGAACTCTTCCCAGTCGGACTTCAGCGCGACGGAGGCCG
ACCCGCGCTGGATGGATGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MADTVLIDSRDGIATLTLNRPEKLNALNYALIDRFLAILDAIETDSSIRAIILTGAGERAFSAGGDIYEFSESVAQGADV
AMRDFVARGQRLTARLEAFRKPVIAAVNGLAFGGGCEITEAVPLAIASECALFAKPEINLAMPPTFGGTQRLPRLAGRKR
ALELLLTGDAFSPQRALELGLVNQVVPHDALMPAAHDLARRIRRHSPLAAASILTAVTRGINQSIAEGLLIEGEQFARMA
PTADLREGLDGWIERRKPNYPGSWSLD

Sequences:

>Translated_267_residues
MADTVLIDSRDGIATLTLNRPEKLNALNYALIDRFLAILDAIETDSSIRAIILTGAGERAFSAGGDIYEFSESVAQGADV
AMRDFVARGQRLTARLEAFRKPVIAAVNGLAFGGGCEITEAVPLAIASECALFAKPEINLAMPPTFGGTQRLPRLAGRKR
ALELLLTGDAFSPQRALELGLVNQVVPHDALMPAAHDLARRIRRHSPLAAASILTAVTRGINQSIAEGLLIEGEQFARMA
PTADLREGLDGWIERRKPNYPGSWSLD
>Mature_266_residues
ADTVLIDSRDGIATLTLNRPEKLNALNYALIDRFLAILDAIETDSSIRAIILTGAGERAFSAGGDIYEFSESVAQGADVA
MRDFVARGQRLTARLEAFRKPVIAAVNGLAFGGGCEITEAVPLAIASECALFAKPEINLAMPPTFGGTQRLPRLAGRKRA
LELLLTGDAFSPQRALELGLVNQVVPHDALMPAAHDLARRIRRHSPLAAASILTAVTRGINQSIAEGLLIEGEQFARMAP
TADLREGLDGWIERRKPNYPGSWSLD

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=38.4920634920635, Blast_Score=164, Evalue=5e-41,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=31.0756972111554, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=28.8, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=32.5670498084291, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI37594469, Length=197, Percent_Identity=32.994923857868, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI37594471, Length=197, Percent_Identity=32.994923857868, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=32.7683615819209, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI150378541, Length=257, Percent_Identity=29.1828793774319, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI68989263, Length=182, Percent_Identity=34.6153846153846, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260274832, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757966, Length=182, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=182, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=182, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=182, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=182, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI49355787, Length=182, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI213417737, Length=183, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI157694516, Length=183, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=38.7596899224806, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=34.9624060150376, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=37, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=32.4817518248175, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=30.7692307692308, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790281, Length=240, Percent_Identity=30.4166666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=28.740157480315, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=38.3399209486166, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=36.6141732283465, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=31.8965517241379, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=207, Percent_Identity=32.8502415458937, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=32.6086956521739, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=28.2926829268293, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=24.812030075188, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=25.5411255411255, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=190, Percent_Identity=30.5263157894737, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=25.6704980842912, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=235, Percent_Identity=29.3617021276596, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=38.7351778656126, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=38.7351778656126, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=33.7209302325581, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=29.7297297297297, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571729, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571730, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=26.5151515151515, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=24.6212121212121, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24654903, Length=210, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=31.5508021390374, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=31.5508021390374, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=31.5508021390374, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650670, Length=210, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921018, Length=188, Percent_Identity=34.0425531914894, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28696; Mature: 28565

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTVLIDSRDGIATLTLNRPEKLNALNYALIDRFLAILDAIETDSSIRAIILTGAGERA
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
FSAGGDIYEFSESVAQGADVAMRDFVARGQRLTARLEAFRKPVIAAVNGLAFGGGCEITE
HCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHH
AVPLAIASECALFAKPEINLAMPPTFGGTQRLPRLAGRKRALELLLTGDAFSPQRALELG
HHHHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHH
LVNQVVPHDALMPAAHDLARRIRRHSPLAAASILTAVTRGINQSIAEGLLIEGEQFARMA
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHC
PTADLREGLDGWIERRKPNYPGSWSLD
CCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ADTVLIDSRDGIATLTLNRPEKLNALNYALIDRFLAILDAIETDSSIRAIILTGAGERA
CCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
FSAGGDIYEFSESVAQGADVAMRDFVARGQRLTARLEAFRKPVIAAVNGLAFGGGCEITE
HCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHH
AVPLAIASECALFAKPEINLAMPPTFGGTQRLPRLAGRKRALELLLTGDAFSPQRALELG
HHHHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHH
LVNQVVPHDALMPAAHDLARRIRRHSPLAAASILTAVTRGINQSIAEGLLIEGEQFARMA
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHC
PTADLREGLDGWIERRKPNYPGSWSLD
CCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]