Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 114800553

GI number: 114800553

Start: 2171482

End: 2172906

Strand: Reverse

Name: pyk [H]

Synonym: HNE_2098

Alternate gene names: 114800553

Gene position: 2172906-2171482 (Counterclockwise)

Preceding gene: 114799087

Following gene: 114800060

Centisome position: 58.65

GC content: 63.93

Gene sequence:

>1425_bases
ATGATCAAATCCCATCTGCAGGGCGAGAATGCCAAGATTGTCGCCACGCTGGGGCCAGGCAGCCGTTCGCCTAGGGAAGT
GCGTGCACTGGCGGAGGCGGGCGTCGATGTGTTCCGCCTGAATTTCAGCCATGGCGAACACGCGGCCCATCTTGAAGCCC
TGAAGGCGGTGCGCGCCGCAGAAGCCGCTGTCGGCTGGCCGCTCGCGACGCTTGCAGATCTTCAGGGACCTAAGGTGCGT
GTTGGCAAGTTCGACGGTGGTTCGCTGAAGCTGGGCTTTCGCAAGGAATACCGCATCATTGTTGGCGAAACGGCACCGGA
CCCGGAAACGATCCCGGTACCCCATGCCGAGATTGTTGCCATCCTTGAGGAGGGCGACACGATCCTGGCCGATGACGGCA
AGCTGATCTTCACCGTGATCAGTGGCGGCAGCGAGCCGCGCGTACGCGCTGAAGTCCCCGGCAAGCTGGGCGACAAGAAG
GGCTTTACCGTTCGGGGCAAGGCGCTGCCGGTCCGTGCACTGACGGAGAAGGACCGCGCTGACCTGGACTTCGCGCTGGA
AATCGGCGTGGACATTGTCGCGCTGTCCTTCGTTCAAACGGTTGAGGATGTTGAGGAGGTCAAGGCGATCATTGCCGGTC
GCGCGCCTCTGGTGGCAAAGCTCGAGAAGCCTGCCGCGATCATTCATCTGGAAGCAATTGTCGCGGCGGCTGACGCGGTA
ATGGTGGCGCGAGGCGATCTGGGCGTTGAGTTTGCACCCGAGGAGGTCCCGGTCATTCAGCGTCGCATCGTGCGCGTAGC
GCGTGCTCTGGGGCGGCCGGTAATCGTGGCGACGCAGATGCTGGAATCGATGATCGAGAATTCTGCGCCCACGCGGGCTG
AAGCCTCGGACGTGGCGACGGCGATCTATCAGGGCGCGGACGCTGTCATGCTGTCCGCCGAGACGGCTGTGGGGCGCCAT
CCGGCAACGGCGGTGGCGATCATGTCCCGCATCATACGGGCCACGGAAGGCGCGGATGACTATCGCCGCTCGCTTGCCGA
GTTCTGCGGAGAGGCTCAGGCCGAAAATGCGATCGACATTGTTGCGCAGGCTGCGCTCACGATGGCGGAAGCGGAAGGCG
CTGCCGCGCTTGCCCTGCGCACCGGCGCATTCGAGCGGCTGGCGCGGTTCGCCCGTGTGCGCGGGTGCGTACCGATTCTT
TACGGATCGCTCGACGATCAGCGGCTTCGCCAGGCGTGTCTTCTCTGGGGCGTTCACCCCCAAAGGCTGAATGCAGGCAC
GGAGAACTGGTATCGCGACCTGATGAAGGCAGCTGGCCTGGAAGGCCGTGTCACCTACGCACGCTGGTCAGGAGATGAGG
AACGCTTCGCCTGGGAAATCGGGGTGGGCCGCGGCGCGGACGGCAAGCCGATTACTGGCGTATGA

Upstream 100 bases:

>100_bases
GCGGAGAGGGAAATGCGCGGCGCCTGAGAAGTGTTTCGCGGATGCCGAGAGTCGTTGTAGCCAGACTGCCTGAAGCGCCC
GCAAACGAAGGAACTGCCCG

Downstream 100 bases:

>100_bases
TCAGCTGGCCTTTGCGGAACTCGTAGGAGTTCAGCTCGCTGAGGAAAGTCATGCCCAGCAGGGACTGTTCCAGCTCGCTG
CGAAGAACGACCGCATTCAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIKSHLQGENAKIVATLGPGSRSPREVRALAEAGVDVFRLNFSHGEHAAHLEALKAVRAAEAAVGWPLATLADLQGPKVR
VGKFDGGSLKLGFRKEYRIIVGETAPDPETIPVPHAEIVAILEEGDTILADDGKLIFTVISGGSEPRVRAEVPGKLGDKK
GFTVRGKALPVRALTEKDRADLDFALEIGVDIVALSFVQTVEDVEEVKAIIAGRAPLVAKLEKPAAIIHLEAIVAAADAV
MVARGDLGVEFAPEEVPVIQRRIVRVARALGRPVIVATQMLESMIENSAPTRAEASDVATAIYQGADAVMLSAETAVGRH
PATAVAIMSRIIRATEGADDYRRSLAEFCGEAQAENAIDIVAQAALTMAEAEGAAALALRTGAFERLARFARVRGCVPIL
YGSLDDQRLRQACLLWGVHPQRLNAGTENWYRDLMKAAGLEGRVTYARWSGDEERFAWEIGVGRGADGKPITGV

Sequences:

>Translated_474_residues
MIKSHLQGENAKIVATLGPGSRSPREVRALAEAGVDVFRLNFSHGEHAAHLEALKAVRAAEAAVGWPLATLADLQGPKVR
VGKFDGGSLKLGFRKEYRIIVGETAPDPETIPVPHAEIVAILEEGDTILADDGKLIFTVISGGSEPRVRAEVPGKLGDKK
GFTVRGKALPVRALTEKDRADLDFALEIGVDIVALSFVQTVEDVEEVKAIIAGRAPLVAKLEKPAAIIHLEAIVAAADAV
MVARGDLGVEFAPEEVPVIQRRIVRVARALGRPVIVATQMLESMIENSAPTRAEASDVATAIYQGADAVMLSAETAVGRH
PATAVAIMSRIIRATEGADDYRRSLAEFCGEAQAENAIDIVAQAALTMAEAEGAAALALRTGAFERLARFARVRGCVPIL
YGSLDDQRLRQACLLWGVHPQRLNAGTENWYRDLMKAAGLEGRVTYARWSGDEERFAWEIGVGRGADGKPITGV
>Mature_474_residues
MIKSHLQGENAKIVATLGPGSRSPREVRALAEAGVDVFRLNFSHGEHAAHLEALKAVRAAEAAVGWPLATLADLQGPKVR
VGKFDGGSLKLGFRKEYRIIVGETAPDPETIPVPHAEIVAILEEGDTILADDGKLIFTVISGGSEPRVRAEVPGKLGDKK
GFTVRGKALPVRALTEKDRADLDFALEIGVDIVALSFVQTVEDVEEVKAIIAGRAPLVAKLEKPAAIIHLEAIVAAADAV
MVARGDLGVEFAPEEVPVIQRRIVRVARALGRPVIVATQMLESMIENSAPTRAEASDVATAIYQGADAVMLSAETAVGRH
PATAVAIMSRIIRATEGADDYRRSLAEFCGEAQAENAIDIVAQAALTMAEAEGAAALALRTGAFERLARFARVRGCVPIL
YGSLDDQRLRQACLLWGVHPQRLNAGTENWYRDLMKAAGLEGRVTYARWSGDEERFAWEIGVGRGADGKPITGV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=432, Percent_Identity=34.4907407407407, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI33286420, Length=432, Percent_Identity=34.4907407407407, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI33286418, Length=432, Percent_Identity=34.0277777777778, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI10835121, Length=428, Percent_Identity=35.2803738317757, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI32967597, Length=428, Percent_Identity=35.2803738317757, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=32.6460481099656, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=32.6460481099656, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.4893617021277, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.4893617021277, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128738, Length=219, Percent_Identity=30.1369863013699, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1788160, Length=427, Percent_Identity=37.0023419203747, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787965, Length=442, Percent_Identity=35.7466063348416, Blast_Score=225, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17544584, Length=458, Percent_Identity=32.9694323144105, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI71984413, Length=460, Percent_Identity=32.8260869565217, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17506829, Length=460, Percent_Identity=32.8260869565217, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71984406, Length=460, Percent_Identity=32.8260869565217, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17506831, Length=460, Percent_Identity=32.8260869565217, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319279, Length=422, Percent_Identity=32.9383886255924, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324923, Length=452, Percent_Identity=32.3008849557522, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24648966, Length=436, Percent_Identity=32.7981651376147, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24648964, Length=439, Percent_Identity=31.8906605922551, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28571814, Length=439, Percent_Identity=31.8906605922551, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=27.0833333333333, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24646914, Length=289, Percent_Identity=32.5259515570934, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50568; Mature: 50568

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSHLQGENAKIVATLGPGSRSPREVRALAEAGVDVFRLNFSHGEHAAHLEALKAVRAA
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
EAAVGWPLATLADLQGPKVRVGKFDGGSLKLGFRKEYRIIVGETAPDPETIPVPHAEIVA
HHHHCCCHHHHHHCCCCEEEEEECCCCEEEEECCEEEEEEEECCCCCCCCCCCCCHHEEE
ILEEGDTILADDGKLIFTVISGGSEPRVRAEVPGKLGDKKGFTVRGKALPVRALTEKDRA
EEECCCEEEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHCCCCCC
DLDFALEIGVDIVALSFVQTVEDVEEVKAIIAGRAPLVAKLEKPAAIIHLEAIVAAADAV
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHHHHHHHHEE
MVARGDLGVEFAPEEVPVIQRRIVRVARALGRPVIVATQMLESMIENSAPTRAEASDVAT
EEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AIYQGADAVMLSAETAVGRHPATAVAIMSRIIRATEGADDYRRSLAEFCGEAQAENAIDI
HHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
VAQAALTMAEAEGAAALALRTGAFERLARFARVRGCVPILYGSLDDQRLRQACLLWGVHP
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHCCCH
QRLNAGTENWYRDLMKAAGLEGRVTYARWSGDEERFAWEIGVGRGADGKPITGV
HHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIKSHLQGENAKIVATLGPGSRSPREVRALAEAGVDVFRLNFSHGEHAAHLEALKAVRAA
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
EAAVGWPLATLADLQGPKVRVGKFDGGSLKLGFRKEYRIIVGETAPDPETIPVPHAEIVA
HHHHCCCHHHHHHCCCCEEEEEECCCCEEEEECCEEEEEEEECCCCCCCCCCCCCHHEEE
ILEEGDTILADDGKLIFTVISGGSEPRVRAEVPGKLGDKKGFTVRGKALPVRALTEKDRA
EEECCCEEEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHCCCCCC
DLDFALEIGVDIVALSFVQTVEDVEEVKAIIAGRAPLVAKLEKPAAIIHLEAIVAAADAV
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHHHHHHHHEE
MVARGDLGVEFAPEEVPVIQRRIVRVARALGRPVIVATQMLESMIENSAPTRAEASDVAT
EEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AIYQGADAVMLSAETAVGRHPATAVAIMSRIIRATEGADDYRRSLAEFCGEAQAENAIDI
HHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
VAQAALTMAEAEGAAALALRTGAFERLARFARVRGCVPILYGSLDDQRLRQACLLWGVHP
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHCCCH
QRLNAGTENWYRDLMKAAGLEGRVTYARWSGDEERFAWEIGVGRGADGKPITGV
HHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8672817 [H]