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The map label for this gene is merA [H]

Identifier: 114800416

GI number: 114800416

Start: 407462

End: 408895

Strand: Reverse

Name: merA [H]

Synonym: HNE_0408

Alternate gene names: 114800416

Gene position: 408895-407462 (Counterclockwise)

Preceding gene: 114799681

Following gene: 114798787

Centisome position: 11.04

GC content: 63.67

Gene sequence:

>1434_bases
ATGTCTGAACTCCGTACCCTGAAGGCTGATCTGGCCGTGATCGGCGCAGGCTCCGCCGGCCTCTCTGCGGCAGCGGGCGC
TGCGATGCTGGGCCTCAAGGTGGTCCTCTTCGAGAAACATGAGATGGGCGGAGACTGCCTGAATTTCGGCTGCGTACCCT
CCAAGGCGCTGATCTCGGCCGCCAAGATTGCCCATGTGCCCGAAGAGGCCGTCCGCTATGGCATCTCCCTGCCCCCGGCG
GTGGTGAACTGGGACGCGGTGAAAGCGCATGTGCGCGGCGCGATCGAGACCATCGCCCCCATCGACAGCCAGGAACGCTT
CGAGGGCCTTGGCTGCACCGTCATCCGCGAAGCCGCGCGCTTTGAGGACAAGAACACGCTCGTCTCTGACAGCGTGCGCG
TGAAGGCGCGGCGCATCATCATCTCCACCGGCAGCCGGGCGATCATCCCGCCCGTGCCGGGCCTGGAAGACGTGCCCTAT
TTCACCAATGAGACCATCTTCTCGGCGCCGGACTTTCCCCATGAGCTGATCATCCTCGGCGGTGGCCCCATAGGGCTGGA
GCTGGCGCAGGCGTTCTCCCGGCTCGGCTCCAAGGTCACCGTTGTGGAGATGGGCCGCGCGCTGCCCCGCTCTGACGCCG
CCCATGCAAAAATCGCCGTTGACGCGGTGCGCGCCGAAGGCGTGACGATCCTGGAAGGGCACAAGGCCACGCGTATTTCC
GGCGGGCCGGGCAATATCAGCGTCAAGGCCGAAGGGCCTGAGGGCGACGTTATGATCGCGGGCAGCCATATCCTGATCGC
CACAGGCCGCCAGGCGGTGACCGATGGCCTCGACCTGGAAAAAGGCGGCGTCGACTTCACCAACAAAGGCGTCACCGTCA
GCGACACCCTCCGCTCAAAGAGCAATCCGCGCGTCTGGGCGCTGGGCGATATTGCCGGCCATGGCCAGTTTACGCATCTG
GCCGGCTGGCATTCCTCGGTGTTCGTGCGCCGGGCGTTTTTCAAGCAGGGCTCGAAAGCCTCAAGCCTGCCCCTGCCAGC
GGTGACCTATACCTCACCGGAAGTCGCGCAGGTCGGCCTCACCGAAGCCGAAGCGCGGGAAAAATTCGGCGACGCAGTTA
AAACCTCTGCCTTCCCTTTTCATGACAATGACCGCGCGATTGCGGAGGCCAAAACGCTGGGCGAAGCCAAGCTCGTAATC
CACAAAGGCAAGCTGGTCGGCGCCTCGATCGTCGGCGAAGGCGCAGGCGACATTATCCAGATGGTGGGCCTGGCGATGTC
CAACGGTCTGAAGCTGACGGCGCTGACGAATTTCATCTCGCCCTATCCCACACGCACCGAAGTGGTGAAGCGGGCGGCTT
CTGCCTATTTCACACCCAGCGTGTTTGGCGCCCCTGCCCGAACACTGGTCGGCATTCTGCAAAAAATACCCTGA

Upstream 100 bases:

>100_bases
TCGCTCGCCTCGAATTTCCTGCCTGCCTTCATTGCGCTCGGCGTGGTGTCGCTGATCCCGGCAGTCTACAAGAAACTTTT
CCCCAAGAAGGCGCCTGCCA

Downstream 100 bases:

>100_bases
GCATTGCTCAGCTTCGGGAGCCCGTTTCCCTTGCGGCGGTTTTGGCATAAGTCTCCGCCCGTGAGTGAGCCTGGCCCTTC
CCCCACCCCTGCCCCGCAGC

Product: pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSELRTLKADLAVIGAGSAGLSAAAGAAMLGLKVVLFEKHEMGGDCLNFGCVPSKALISAAKIAHVPEEAVRYGISLPPA
VVNWDAVKAHVRGAIETIAPIDSQERFEGLGCTVIREAARFEDKNTLVSDSVRVKARRIIISTGSRAIIPPVPGLEDVPY
FTNETIFSAPDFPHELIILGGGPIGLELAQAFSRLGSKVTVVEMGRALPRSDAAHAKIAVDAVRAEGVTILEGHKATRIS
GGPGNISVKAEGPEGDVMIAGSHILIATGRQAVTDGLDLEKGGVDFTNKGVTVSDTLRSKSNPRVWALGDIAGHGQFTHL
AGWHSSVFVRRAFFKQGSKASSLPLPAVTYTSPEVAQVGLTEAEAREKFGDAVKTSAFPFHDNDRAIAEAKTLGEAKLVI
HKGKLVGASIVGEGAGDIIQMVGLAMSNGLKLTALTNFISPYPTRTEVVKRAASAYFTPSVFGAPARTLVGILQKIP

Sequences:

>Translated_477_residues
MSELRTLKADLAVIGAGSAGLSAAAGAAMLGLKVVLFEKHEMGGDCLNFGCVPSKALISAAKIAHVPEEAVRYGISLPPA
VVNWDAVKAHVRGAIETIAPIDSQERFEGLGCTVIREAARFEDKNTLVSDSVRVKARRIIISTGSRAIIPPVPGLEDVPY
FTNETIFSAPDFPHELIILGGGPIGLELAQAFSRLGSKVTVVEMGRALPRSDAAHAKIAVDAVRAEGVTILEGHKATRIS
GGPGNISVKAEGPEGDVMIAGSHILIATGRQAVTDGLDLEKGGVDFTNKGVTVSDTLRSKSNPRVWALGDIAGHGQFTHL
AGWHSSVFVRRAFFKQGSKASSLPLPAVTYTSPEVAQVGLTEAEAREKFGDAVKTSAFPFHDNDRAIAEAKTLGEAKLVI
HKGKLVGASIVGEGAGDIIQMVGLAMSNGLKLTALTNFISPYPTRTEVVKRAASAYFTPSVFGAPARTLVGILQKIP
>Mature_476_residues
SELRTLKADLAVIGAGSAGLSAAAGAAMLGLKVVLFEKHEMGGDCLNFGCVPSKALISAAKIAHVPEEAVRYGISLPPAV
VNWDAVKAHVRGAIETIAPIDSQERFEGLGCTVIREAARFEDKNTLVSDSVRVKARRIIISTGSRAIIPPVPGLEDVPYF
TNETIFSAPDFPHELIILGGGPIGLELAQAFSRLGSKVTVVEMGRALPRSDAAHAKIAVDAVRAEGVTILEGHKATRISG
GPGNISVKAEGPEGDVMIAGSHILIATGRQAVTDGLDLEKGGVDFTNKGVTVSDTLRSKSNPRVWALGDIAGHGQFTHLA
GWHSSVFVRRAFFKQGSKASSLPLPAVTYTSPEVAQVGLTEAEAREKFGDAVKTSAFPFHDNDRAIAEAKTLGEAKLVIH
KGKLVGASIVGEGAGDIIQMVGLAMSNGLKLTALTNFISPYPTRTEVVKRAASAYFTPSVFGAPARTLVGILQKIP

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=480, Percent_Identity=29.1666666666667, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=27.5488069414317, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI148277065, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI148277071, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI33519430, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=24.7826086956522, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI22035672, Length=466, Percent_Identity=24.4635193133047, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI291045268, Length=455, Percent_Identity=24.1758241758242, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=30.5010893246187, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=29.7777777777778, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=29.1954022988506, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=24.8380129589633, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=28.6919831223629, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=27.2151898734177, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=27.4028629856851, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=429, Percent_Identity=25.1748251748252, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=429, Percent_Identity=25.1748251748252, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=492, Percent_Identity=31.5040650406504, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=27.3504273504274, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=24.6315789473684, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358499, Length=484, Percent_Identity=29.7520661157025, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=26.2939958592132, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=26.2939958592132, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=26.25, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=25.2083333333333, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49971; Mature: 49840

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELRTLKADLAVIGAGSAGLSAAAGAAMLGLKVVLFEKHEMGGDCLNFGCVPSKALISA
CCHHHHHHCCEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCCCCEEECCCCCHHHHHHH
AKIAHVPEEAVRYGISLPPAVVNWDAVKAHVRGAIETIAPIDSQERFEGLGCTVIREAAR
HHHHCCCHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHH
FEDKNTLVSDSVRVKARRIIISTGSRAIIPPVPGLEDVPYFTNETIFSAPDFPHELIILG
CCCCCCEECCCEEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCEEECCCCCCCEEEEEC
GGPIGLELAQAFSRLGSKVTVVEMGRALPRSDAAHAKIAVDAVRAEGVTILEGHKATRIS
CCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEHHCCCEEEEECCCEEEEC
GGPGNISVKAEGPEGDVMIAGSHILIATGRQAVTDGLDLEKGGVDFTNKGVTVSDTLRSK
CCCCCEEEEECCCCCCEEEECCEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEHHHHCC
SNPRVWALGDIAGHGQFTHLAGWHSSVFVRRAFFKQGSKASSLPLPAVTYTSPEVAQVGL
CCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHCCC
TEAEAREKFGDAVKTSAFPFHDNDRAIAEAKTLGEAKLVIHKGKLVGASIVGEGAGDIIQ
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHH
MVGLAMSNGLKLTALTNFISPYPTRTEVVKRAASAYFTPSVFGAPARTLVGILQKIP
HHHHHHCCCEEEEEEHHHHCCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SELRTLKADLAVIGAGSAGLSAAAGAAMLGLKVVLFEKHEMGGDCLNFGCVPSKALISA
CHHHHHHCCEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCCCCEEECCCCCHHHHHHH
AKIAHVPEEAVRYGISLPPAVVNWDAVKAHVRGAIETIAPIDSQERFEGLGCTVIREAAR
HHHHCCCHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHH
FEDKNTLVSDSVRVKARRIIISTGSRAIIPPVPGLEDVPYFTNETIFSAPDFPHELIILG
CCCCCCEECCCEEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCEEECCCCCCCEEEEEC
GGPIGLELAQAFSRLGSKVTVVEMGRALPRSDAAHAKIAVDAVRAEGVTILEGHKATRIS
CCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEHHCCCEEEEECCCEEEEC
GGPGNISVKAEGPEGDVMIAGSHILIATGRQAVTDGLDLEKGGVDFTNKGVTVSDTLRSK
CCCCCEEEEECCCCCCEEEECCEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEHHHHCC
SNPRVWALGDIAGHGQFTHLAGWHSSVFVRRAFFKQGSKASSLPLPAVTYTSPEVAQVGL
CCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHCCC
TEAEAREKFGDAVKTSAFPFHDNDRAIAEAKTLGEAKLVIHKGKLVGASIVGEGAGDIIQ
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHH
MVGLAMSNGLKLTALTNFISPYPTRTEVVKRAASAYFTPSVFGAPARTLVGILQKIP
HHHHHHCCCEEEEEEHHHHCCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1494353 [H]