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The map label for this gene is fixL [H]

Identifier: 114798787

GI number: 114798787

Start: 405977

End: 407401

Strand: Reverse

Name: fixL [H]

Synonym: HNE_0407

Alternate gene names: 114798787

Gene position: 407401-405977 (Counterclockwise)

Preceding gene: 114800416

Following gene: 114797970

Centisome position: 11.0

GC content: 68.98

Gene sequence:

>1425_bases
GTGAGTGAGCCTGGCCCTTCCCCCACCCCTGCCCCGCAGCGCCGGTTCCGGCGCCTGCCGGAGAGCCTGGCTGCGCGCCT
CTTTGCGCTGACGCTGGGCGCCATCCTGCTGACAGAGGCGCTGATCTTCGTACCCTCGGCCAGCAATGCGCGCACCCAGT
GGCTGAGCGAGCGGGTGCAGGCCGCCCGGATTGCGGCGCTCGCCCTGGAAGCCGCCCCCATGCGGGAGGTGTCCGAGGAG
CTGTCCGAAAGCCTGCTGGAAAAGGCCGAAGTGCTGGCCGTCACCGAGGTGGAAGACGAGATGCGCTTCCAGCTGCTCGC
CCCGGCCATGCCGGTGGAAGAAGCGCCCACAAGGATCGTGGACTTGCGCGCGCCGACCATGATGGGCCAGAGCGCGGCGG
CCTTGAGCGAATATTTTGCGCCCGAAGGCCAGATGCTGGTGGTGATCGCCGAAGGGTCTGACGCCGGCCGGGTGATCGAA
GTGGTGGTGCCCCAGGCGCCGCTGAAACAGACAATGGTGGACCTTGCCTGGCGCGTAACCGGCCTGTCGCTGATCATTGC
GCTGGTGGCGGCGGCGGTCATCTATCTGGTGCTGGACGTGTTTGTGGTGCGCCCCGTGCGGCGGGTGACCGTGAGCGTGG
AACAGTTCAGCCGGGACCCCGGCAGCTGGACCCGGCGCCTCTCGCCCACAAAGCGGCGCGACGAGATCGGCCGTGCACAG
AATGCCCTCGCCGGAATGGAAGAGGCCGTGGCCGATGCCTTCCGCCAGCGCGCCCACCTTGCCGAGCTCGGCAGCGCCGT
GGCCAAGATCAACCATGATCTGCGCAACTCGCTGGCCTCGGCCCAGCTTGTCTCCGATGGCCTTGCCGCCTCGGAAGACC
CGCGCGTGCAGCGCGCCGCCCCGCGCCTCAAACGCGCCTTGGAACGCGCCATCGAGCTTGCCACCGCCACGCTCGACTAT
GGCAAGGCCGCCCCGCGCGCGCCGGTGATGCAGACCGTGCGCCTGCTCAGCGTGTTGCAAGGCGCCGCAGAAGAAGCGCT
GGTGGAGAACGGCGTGGCCACCGACATCGACGTGCCCGAAACGCTGGTGATCCGCTCAGACGCCGACCATCTGCACCGGA
TTGCGGCCAACCTCATCCGCAACGCCGCTGAGGCGATGCGGCAGGTGGAGACGCCCGCCCCGGAAATCCGGATACGGATT
GAAGACGGCGCGCTGCTGTTTGCCGATAACGGCCCCGGCGTGCCCGAAAAGACGCAGGAAAACCTCTTTCGCCCCTTCTC
CGGCTCTGGCCGCGTGGGCGGCACGGGGCTGGGCCTGGTGATTGCGCATGAACTGGCGCAGGCGCTCGGCGGCAGCCTCA
CACTGGCCCGCACAGGCCCGGAGGGCACCGTCTTCCGGCTCGACCTGCCGGGCCTGAGCGCATGA

Upstream 100 bases:

>100_bases
TGCCCGAACACTGGTCGGCATTCTGCAAAAAATACCCTGAGCATTGCTCAGCTTCGGGAGCCCGTTTCCCTTGCGGCGGT
TTTGGCATAAGTCTCCGCCC

Downstream 100 bases:

>100_bases
GCCATTATGCCCCGGCGCGCGGGCCGGTGTCCTACATCTACCGCGATGACCATCTGATCCTGATCGACAAACCCTCCGGC
CTCCTCTCCGTACCCGGACG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSEPGPSPTPAPQRRFRRLPESLAARLFALTLGAILLTEALIFVPSASNARTQWLSERVQAARIAALALEAAPMREVSEE
LSESLLEKAEVLAVTEVEDEMRFQLLAPAMPVEEAPTRIVDLRAPTMMGQSAAALSEYFAPEGQMLVVIAEGSDAGRVIE
VVVPQAPLKQTMVDLAWRVTGLSLIIALVAAAVIYLVLDVFVVRPVRRVTVSVEQFSRDPGSWTRRLSPTKRRDEIGRAQ
NALAGMEEAVADAFRQRAHLAELGSAVAKINHDLRNSLASAQLVSDGLAASEDPRVQRAAPRLKRALERAIELATATLDY
GKAAPRAPVMQTVRLLSVLQGAAEEALVENGVATDIDVPETLVIRSDADHLHRIAANLIRNAAEAMRQVETPAPEIRIRI
EDGALLFADNGPGVPEKTQENLFRPFSGSGRVGGTGLGLVIAHELAQALGGSLTLARTGPEGTVFRLDLPGLSA

Sequences:

>Translated_474_residues
MSEPGPSPTPAPQRRFRRLPESLAARLFALTLGAILLTEALIFVPSASNARTQWLSERVQAARIAALALEAAPMREVSEE
LSESLLEKAEVLAVTEVEDEMRFQLLAPAMPVEEAPTRIVDLRAPTMMGQSAAALSEYFAPEGQMLVVIAEGSDAGRVIE
VVVPQAPLKQTMVDLAWRVTGLSLIIALVAAAVIYLVLDVFVVRPVRRVTVSVEQFSRDPGSWTRRLSPTKRRDEIGRAQ
NALAGMEEAVADAFRQRAHLAELGSAVAKINHDLRNSLASAQLVSDGLAASEDPRVQRAAPRLKRALERAIELATATLDY
GKAAPRAPVMQTVRLLSVLQGAAEEALVENGVATDIDVPETLVIRSDADHLHRIAANLIRNAAEAMRQVETPAPEIRIRI
EDGALLFADNGPGVPEKTQENLFRPFSGSGRVGGTGLGLVIAHELAQALGGSLTLARTGPEGTVFRLDLPGLSA
>Mature_473_residues
SEPGPSPTPAPQRRFRRLPESLAARLFALTLGAILLTEALIFVPSASNARTQWLSERVQAARIAALALEAAPMREVSEEL
SESLLEKAEVLAVTEVEDEMRFQLLAPAMPVEEAPTRIVDLRAPTMMGQSAAALSEYFAPEGQMLVVIAEGSDAGRVIEV
VVPQAPLKQTMVDLAWRVTGLSLIIALVAAAVIYLVLDVFVVRPVRRVTVSVEQFSRDPGSWTRRLSPTKRRDEIGRAQN
ALAGMEEAVADAFRQRAHLAELGSAVAKINHDLRNSLASAQLVSDGLAASEDPRVQRAAPRLKRALERAIELATATLDYG
KAAPRAPVMQTVRLLSVLQGAAEEALVENGVATDIDVPETLVIRSDADHLHRIAANLIRNAAEAMRQVETPAPEIRIRIE
DGALLFADNGPGVPEKTQENLFRPFSGSGRVGGTGLGLVIAHELAQALGGSLTLARTGPEGTVFRLDLPGLSA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=25.8741258741259, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50841; Mature: 50710

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPGPSPTPAPQRRFRRLPESLAARLFALTLGAILLTEALIFVPSASNARTQWLSERVQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
AARIAALALEAAPMREVSEELSESLLEKAEVLAVTEVEDEMRFQLLAPAMPVEEAPTRIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCCCCEE
DLRAPTMMGQSAAALSEYFAPEGQMLVVIAEGSDAGRVIEVVVPQAPLKQTMVDLAWRVT
ECCCCHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHH
GLSLIIALVAAAVIYLVLDVFVVRPVRRVTVSVEQFSRDPGSWTRRLSPTKRRDEIGRAQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHH
NALAGMEEAVADAFRQRAHLAELGSAVAKINHDLRNSLASAQLVSDGLAASEDPRVQRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
PRLKRALERAIELATATLDYGKAAPRAPVMQTVRLLSVLQGAAEEALVENGVATDIDVPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLVIRSDADHLHRIAANLIRNAAEAMRQVETPAPEIRIRIEDGALLFADNGPGVPEKTQE
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCHHHH
NLFRPFSGSGRVGGTGLGLVIAHELAQALGGSLTLARTGPEGTVFRLDLPGLSA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure 
SEPGPSPTPAPQRRFRRLPESLAARLFALTLGAILLTEALIFVPSASNARTQWLSERVQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
AARIAALALEAAPMREVSEELSESLLEKAEVLAVTEVEDEMRFQLLAPAMPVEEAPTRIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCCCCEE
DLRAPTMMGQSAAALSEYFAPEGQMLVVIAEGSDAGRVIEVVVPQAPLKQTMVDLAWRVT
ECCCCHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHH
GLSLIIALVAAAVIYLVLDVFVVRPVRRVTVSVEQFSRDPGSWTRRLSPTKRRDEIGRAQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHH
NALAGMEEAVADAFRQRAHLAELGSAVAKINHDLRNSLASAQLVSDGLAASEDPRVQRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
PRLKRALERAIELATATLDYGKAAPRAPVMQTVRLLSVLQGAAEEALVENGVATDIDVPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLVIRSDADHLHRIAANLIRNAAEAMRQVETPAPEIRIRIEDGALLFADNGPGVPEKTQE
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCHHHH
NLFRPFSGSGRVGGTGLGLVIAHELAQALGGSLTLARTGPEGTVFRLDLPGLSA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]