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The map label for this gene is folC [H]

Identifier: 114800401

GI number: 114800401

Start: 3607747

End: 3609000

Strand: Reverse

Name: folC [H]

Synonym: HNE_3472

Alternate gene names: 114800401

Gene position: 3609000-3607747 (Counterclockwise)

Preceding gene: 114797289

Following gene: 114799429

Centisome position: 97.41

GC content: 65.55

Gene sequence:

>1254_bases
TTGAGCGGCAATTCACCGGCCGTTGATGCCGCGCTGAAGCGGTTTGAAGGCCTCTATCCCGATACTATCGAGCTTTCGCT
CGGGCGCATCGAGCATGCGCTCGACGCGCTGGGCCGTCCGCAGGACCGTCTGCCGCCGGTAATCCATGTAGCGGGCACGA
ACGGGAAGGGGTCTACCTGCGCCTTCATGCGTGCGATGGCCGAAGCGGCGGGGCTGAAAGTGCATGTGTTCACCAGCCCG
CATCTGGTGCGGTTCAATGAACGTATCCGGCTCGCCGGGAAGCTTGTGGAAGACGCGGCGCTGATCGGCTGGCTTGACCG
GACGTTTGAGGCCATCGGGGGCGGGCAGATCACGCATTTCGAGGCGACGACAGCGGCTGCGTTGCTGGCCTTCTCGGAAG
TGCCAGCGGATGTGCTGATCCTGGAAGTGGGCCTTGGCGGGAAATATGACGCGACGAATGTGATCGGCGCGCCAGCGCTC
TCGGTGATTACGCCGGTGGACTACGATCATGCGCAATTTCTTGGGACTGATCTTGCCGGGATCGCGGCGGAGAAGGCGGG
CATCATCAAGGCCGGGCGCCCGGCGCTGACATCTATCCAGCAGGATGTGGCCGACGCCGTGATTACAGCGCGGGCGGCAG
AAGTGGGCGCGCCACTTTACCGGCTGAAACCGCATTTCATCGAGGCAATGCCTGAGGAGGTTGCGCTGAGTGGGCGCCAT
CAGCGGGCCAATGCCGCGCTGGCGGCGATGGCAATGCAGCTGTTCGGGCAGTCTGTGCGGATTGATCAGGCGGCGATCTT
CAAGGGCGCGCGTGAGGCTGTGTGGCCGGCGCGGATGCAGCGGCTGAAGGACGGGCCGGTGACGGCGCTGGCGGGCGGAT
TGCCGGTCTGGCTCGATGGCGGACACAATCCCCATGCGGCGCGGGCAATTGCGCTGCATCTCGATGGGCTCGGCGGGAAG
ACGGCGCTGGTCTCGGCGATGCTGGCGAGCAAGGATTCGGCGGGGTTTTTCGTGCCGTTTGCGCCGCTGGGCGTCGAGGT
GTTCACCTGCCCAAATGCGCCGGGCCATCAGGCCATGCCGCCCGCTGAACTTGCGCAGGCGGCCTCGCGGGCTGGCCTGC
GTGTGACGGCCTGTACTAGCTTTCAGGACGCCATGCGGGCCGCCGCTGCCAGCCGCCCGGACCGGTTACTGATCTGCGGC
TCGCTCTACCTGGCAGGTGAAGTGTTGGCCATGAACGGAGAAGCGCCGGAGTAG

Upstream 100 bases:

>100_bases
TGCTGCCGATGTCGCGCCGGCGGGATGATCGCTCGACGCTGCAGCTGACGCCGCCAAAAACGCATGCGCCCAAGCCGCCC
GAGCCGAAGGTCAAGCCTGA

Downstream 100 bases:

>100_bases
GGGAGGGCGCATAAACCGCGCCAATTTGACCCTCACGTAAGCGTCAAGCCTTGCGCGCGGGGCGCCCTATATGTAGGGAC
AGCGCACTTTAAAGTGCCAA

Product: putative folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGNSPAVDAALKRFEGLYPDTIELSLGRIEHALDALGRPQDRLPPVIHVAGTNGKGSTCAFMRAMAEAAGLKVHVFTSP
HLVRFNERIRLAGKLVEDAALIGWLDRTFEAIGGGQITHFEATTAAALLAFSEVPADVLILEVGLGGKYDATNVIGAPAL
SVITPVDYDHAQFLGTDLAGIAAEKAGIIKAGRPALTSIQQDVADAVITARAAEVGAPLYRLKPHFIEAMPEEVALSGRH
QRANAALAAMAMQLFGQSVRIDQAAIFKGAREAVWPARMQRLKDGPVTALAGGLPVWLDGGHNPHAARAIALHLDGLGGK
TALVSAMLASKDSAGFFVPFAPLGVEVFTCPNAPGHQAMPPAELAQAASRAGLRVTACTSFQDAMRAAAASRPDRLLICG
SLYLAGEVLAMNGEAPE

Sequences:

>Translated_417_residues
MSGNSPAVDAALKRFEGLYPDTIELSLGRIEHALDALGRPQDRLPPVIHVAGTNGKGSTCAFMRAMAEAAGLKVHVFTSP
HLVRFNERIRLAGKLVEDAALIGWLDRTFEAIGGGQITHFEATTAAALLAFSEVPADVLILEVGLGGKYDATNVIGAPAL
SVITPVDYDHAQFLGTDLAGIAAEKAGIIKAGRPALTSIQQDVADAVITARAAEVGAPLYRLKPHFIEAMPEEVALSGRH
QRANAALAAMAMQLFGQSVRIDQAAIFKGAREAVWPARMQRLKDGPVTALAGGLPVWLDGGHNPHAARAIALHLDGLGGK
TALVSAMLASKDSAGFFVPFAPLGVEVFTCPNAPGHQAMPPAELAQAASRAGLRVTACTSFQDAMRAAAASRPDRLLICG
SLYLAGEVLAMNGEAPE
>Mature_416_residues
SGNSPAVDAALKRFEGLYPDTIELSLGRIEHALDALGRPQDRLPPVIHVAGTNGKGSTCAFMRAMAEAAGLKVHVFTSPH
LVRFNERIRLAGKLVEDAALIGWLDRTFEAIGGGQITHFEATTAAALLAFSEVPADVLILEVGLGGKYDATNVIGAPALS
VITPVDYDHAQFLGTDLAGIAAEKAGIIKAGRPALTSIQQDVADAVITARAAEVGAPLYRLKPHFIEAMPEEVALSGRHQ
RANAALAAMAMQLFGQSVRIDQAAIFKGAREAVWPARMQRLKDGPVTALAGGLPVWLDGGHNPHAARAIALHLDGLGGKT
ALVSAMLASKDSAGFFVPFAPLGVEVFTCPNAPGHQAMPPAELAQAASRAGLRVTACTSFQDAMRAAAASRPDRLLICGS
LYLAGEVLAMNGEAPE

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=325, Percent_Identity=33.8461538461538, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI66932984, Length=325, Percent_Identity=33.8461538461538, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1788654, Length=418, Percent_Identity=31.3397129186603, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71984923, Length=482, Percent_Identity=26.1410788381743, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=482, Percent_Identity=26.1410788381743, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17553148, Length=485, Percent_Identity=26.8041237113402, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=28.7410926365796, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=280, Percent_Identity=28.9285714285714, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641571, Length=277, Percent_Identity=31.0469314079422, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581568, Length=183, Percent_Identity=34.4262295081967, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 43530; Mature: 43398

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNSPAVDAALKRFEGLYPDTIELSLGRIEHALDALGRPQDRLPPVIHVAGTNGKGSTC
CCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCCCCHH
AFMRAMAEAAGLKVHVFTSPHLVRFNERIRLAGKLVEDAALIGWLDRTFEAIGGGQITHF
HHHHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EATTAAALLAFSEVPADVLILEVGLGGKYDATNVIGAPALSVITPVDYDHAQFLGTDLAG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCHHHH
IAAEKAGIIKAGRPALTSIQQDVADAVITARAAEVGAPLYRLKPHFIEAMPEEVALSGRH
HHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCHHHHHCCCC
QRANAALAAMAMQLFGQSVRIDQAAIFKGAREAVWPARMQRLKDGPVTALAGGLPVWLDG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCHHHHHHCCCCCEEEECCCCEEEECC
GHNPHAARAIALHLDGLGGKTALVSAMLASKDSAGFFVPFAPLGVEVFTCPNAPGHQAMP
CCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCC
PAELAQAASRAGLRVTACTSFQDAMRAAAASRPDRLLICGSLYLAGEVLAMNGEAPE
HHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCEECCEEEEECCCCCC
>Mature Secondary Structure 
SGNSPAVDAALKRFEGLYPDTIELSLGRIEHALDALGRPQDRLPPVIHVAGTNGKGSTC
CCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCCCCHH
AFMRAMAEAAGLKVHVFTSPHLVRFNERIRLAGKLVEDAALIGWLDRTFEAIGGGQITHF
HHHHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EATTAAALLAFSEVPADVLILEVGLGGKYDATNVIGAPALSVITPVDYDHAQFLGTDLAG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCHHHH
IAAEKAGIIKAGRPALTSIQQDVADAVITARAAEVGAPLYRLKPHFIEAMPEEVALSGRH
HHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCHHHHHCCCC
QRANAALAAMAMQLFGQSVRIDQAAIFKGAREAVWPARMQRLKDGPVTALAGGLPVWLDG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCHHHHHHCCCCCEEEECCCCEEEECC
GHNPHAARAIALHLDGLGGKTALVSAMLASKDSAGFFVPFAPLGVEVFTCPNAPGHQAMP
CCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCC
PAELAQAASRAGLRVTACTSFQDAMRAAAASRPDRLLICGSLYLAGEVLAMNGEAPE
HHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]