BacMap

Definition	Hyphomonas neptunium ATCC 15444 chromosome, complete genome.
Accession	NC_008358
Length	3,705,021

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The map label for this gene is paaF [C]

Identifier: 114800390

GI number: 114800390

Start: 1882510

End: 1883307

Strand: Reverse

Name: paaF [C]

Synonym: HNE_1824

Alternate gene names: 114800390

Gene position: 1883307-1882510 (Counterclockwise)

Preceding gene: 114799178

Following gene: 114797813

Centisome position: 50.83

GC content: 61.15

Gene sequence:

>798_bases
ATGTCCATTCTGCTGCTAGAGAAGCGCGGCCCGATTGCCGTGATGACGCTGAACCGTCCGGAAATGATGAATGCGCTGGG
GCAGGAAGGCGACGGTCCGGCAGTGGCCGCCGTGTGCGCCGAGATTGCAGCTGATCCACAGATCCGCTGCGCCATTCTGA
CGGGGGCTGGCCGGGCGTTTTCCGCTGGTGGCGACGTGAAGGCCATGAAGGAAAAGACGGGCGCTTTCGGTGGAAGTCCG
GCAGACATTCGTGAGGGCTATCGCCGCAACATTCACATGATCGTCCGCTCCCTGTACAATATGGAAACACCGCTCATCTC
AGCCATCAATGGCCCGGCCATTGGCCTTGGCTGCGATGTAGCGTGCATGGCCGACATCCGCATTGCGTCCGAGACGGCAA
AGATGGGGGTTACGTTCCTAAAGCTTGGTCTGATCCCCGGCGATGGCGGCGCCTGGCTGTTGCCGCGGTTGATCGGCTCC
TCGCGCGCCGCCGAACTCTTGTTCACCGGGGACGTGATCGACGCGAAGACGGCGGCCGAATGGGGGCTTGTCAGCAAGGT
GGTCCCCGCTGACAAGCTGATGGATGAGGCAATGGCGATGGCGGAGAAGATTGCCGGCCAGCCGCCGCAGGCACTCAGGC
TCGCCAAGACGCTAATGCGCCACGGAACGAACGCCTCCTATGATACGATCATGGAACTTTCGGCGGCGTCGCAGGCGCTG
ATGCATGAGACCGAGGATCATGTCGAAGGCGTCAACGCAATTCTTGAGAAGCGGACGCCCGTTTTCAAAGGCAAGTAA

Upstream 100 bases:

>100_bases
AATGCTTTGCTGGCCCGCCAGCACCGCCGCCGCCGCCGGGCAGCCGGTTCACGGCGAAAGCTGCTGCCACCTGAACATCT
CAACTCCAAAGGAAACGCCC

Downstream 100 bases:

>100_bases
GGTCTCGGCACCGGCGTCATTAAGACGAAAGGGCGTGAGTATTTCTCACGCCCTTTCATTTTGTCAGAATACCACGCCGG
ACTGGTAAAGGGCGTCAATG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSILLLEKRGPIAVMTLNRPEMMNALGQEGDGPAVAAVCAEIAADPQIRCAILTGAGRAFSAGGDVKAMKEKTGAFGGSP
ADIREGYRRNIHMIVRSLYNMETPLISAINGPAIGLGCDVACMADIRIASETAKMGVTFLKLGLIPGDGGAWLLPRLIGS
SRAAELLFTGDVIDAKTAAEWGLVSKVVPADKLMDEAMAMAEKIAGQPPQALRLAKTLMRHGTNASYDTIMELSAASQAL
MHETEDHVEGVNAILEKRTPVFKGK

Sequences:

>Translated_265_residues
MSILLLEKRGPIAVMTLNRPEMMNALGQEGDGPAVAAVCAEIAADPQIRCAILTGAGRAFSAGGDVKAMKEKTGAFGGSP
ADIREGYRRNIHMIVRSLYNMETPLISAINGPAIGLGCDVACMADIRIASETAKMGVTFLKLGLIPGDGGAWLLPRLIGS
SRAAELLFTGDVIDAKTAAEWGLVSKVVPADKLMDEAMAMAEKIAGQPPQALRLAKTLMRHGTNASYDTIMELSAASQAL
MHETEDHVEGVNAILEKRTPVFKGK
>Mature_264_residues
SILLLEKRGPIAVMTLNRPEMMNALGQEGDGPAVAAVCAEIAADPQIRCAILTGAGRAFSAGGDVKAMKEKTGAFGGSPA
DIREGYRRNIHMIVRSLYNMETPLISAINGPAIGLGCDVACMADIRIASETAKMGVTFLKLGLIPGDGGAWLLPRLIGSS
RAAELLFTGDVIDAKTAAEWGLVSKVVPADKLMDEAMAMAEKIAGQPPQALRLAKTLMRHGTNASYDTIMELSAASQALM
HETEDHVEGVNAILEKRTPVFKGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=272, Percent_Identity=33.8235294117647, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=33.7121212121212, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI31542718, Length=220, Percent_Identity=30.9090909090909, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI45643119, Length=242, Percent_Identity=26.4462809917355, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260275230, Length=242, Percent_Identity=26.4462809917355, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260274832, Length=231, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI37594471, Length=258, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=258, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=29.6, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI150378541, Length=259, Percent_Identity=29.3436293436293, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=31.5270935960591, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116235487, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538573, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI20127408, Length=190, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=148, Percent_Identity=29.7297297297297, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221136753, Length=148, Percent_Identity=29.7297297297297, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221307494, Length=130, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4757968, Length=227, Percent_Identity=25.5506607929515, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI49355787, Length=227, Percent_Identity=25.5506607929515, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=220, Percent_Identity=28.6363636363636, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=28.6363636363636, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=33.955223880597, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=37.3584905660377, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=33.2089552238806, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=28.735632183908, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=32.48730964467, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790281, Length=107, Percent_Identity=36.4485981308411, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=279, Percent_Identity=34.0501792114695, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=34, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17535521, Length=218, Percent_Identity=32.1100917431193, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=32.8947368421053, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=26.1044176706827, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=251, Percent_Identity=26.6932270916335, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=28.3422459893048, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=222, Percent_Identity=27.4774774774775, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=271, Percent_Identity=23.2472324723247, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=27.8350515463918, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=27.8350515463918, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141363, Length=205, Percent_Identity=25.8536585365854, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=34.9019607843137, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=34.9019607843137, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=31.2977099236641, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=24.609375, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21357171, Length=248, Percent_Identity=28.6290322580645, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=29.4573643410853, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922422, Length=271, Percent_Identity=21.4022140221402, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27958; Mature: 27827

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILLLEKRGPIAVMTLNRPEMMNALGQEGDGPAVAAVCAEIAADPQIRCAILTGAGRAF
CEEEEEECCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
SAGGDVKAMKEKTGAFGGSPADIREGYRRNIHMIVRSLYNMETPLISAINGPAIGLGCDV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCH
ACMADIRIASETAKMGVTFLKLGLIPGDGGAWLLPRLIGSSRAAELLFTGDVIDAKTAAE
HHHHHHHHHHHHHHHHHEEHEEEEECCCCCCHHHHHHHCCCHHEEEEEECCCCCCHHHHH
WGLVSKVVPADKLMDEAMAMAEKIAGQPPQALRLAKTLMRHGTNASYDTIMELSAASQAL
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MHETEDHVEGVNAILEKRTPVFKGK
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SILLLEKRGPIAVMTLNRPEMMNALGQEGDGPAVAAVCAEIAADPQIRCAILTGAGRAF
EEEEEECCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
SAGGDVKAMKEKTGAFGGSPADIREGYRRNIHMIVRSLYNMETPLISAINGPAIGLGCDV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCH
ACMADIRIASETAKMGVTFLKLGLIPGDGGAWLLPRLIGSSRAAELLFTGDVIDAKTAAE
HHHHHHHHHHHHHHHHHEEHEEEEECCCCCCHHHHHHHCCCHHEEEEEECCCCCCHHHHH
WGLVSKVVPADKLMDEAMAMAEKIAGQPPQALRLAKTLMRHGTNASYDTIMELSAASQAL
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MHETEDHVEGVNAILEKRTPVFKGK
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA