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The map label for this gene is nuoN [H]

Identifier: 114800322

GI number: 114800322

Start: 745440

End: 746936

Strand: Direct

Name: nuoN [H]

Synonym: HNE_0735

Alternate gene names: 114800322

Gene position: 745440-746936 (Clockwise)

Preceding gene: 114799114

Following gene: 114797124

Centisome position: 20.12

GC content: 61.39

Gene sequence:

>1497_bases
TTGGCGCATGCGCCGGTATTGCTGGTGGCAATTCCAATGCTGATTGCGCCTTTGGTGGCGATCCTGCCCAGTGGCCGGCT
GGCCTGGCTGCTCGCTATGGCGACCACTGCAGTCTCTTTTTACCTCGCCATAGTCACGCTGGGCCTCGTTCAGTCTTCGC
CGACGGGCATCCTGTCCTATGAGATCGGCCGGTGGCCCCCGCCGATCGGGATCGAATTCCGGACCGATGCGCTCAATGCG
ATGATCCTTCTGCTGGTAACGGGCATAGGCTTTCTGGCGTCCGTGTTTTCCTGGCCAACGATTTCGTCGGAGCTTGGCAA
TCATAACAAGCTCGGCATGTTCTACGCCGCGTTCCTGCTTTGCCTTGCCGGTTTGCTGGGCGTTGCCTCGACCGGGGACG
CCTTCAACCTCTTCGTATTCCTCGAGATCAGCTCGCTGGCGACATATGTGATGGTTGCCATGGGCGCCCGGCGGGATCGC
CGCGCGCTGCCTGCGGCTTTCAACTATCTGATCATGGGAACGCTCGGGGCGAGCTTTTACATTATCGGCATCGGCTTCCT
TTATGCGGCCACCGGCACGCTCAACATGGCCGACATCGCCGACCGCCTGCCCGCGCTGACCGATAGCCGCTCGGTACAGG
TCGGTTTTGCCTTCATCATCGTCGGTCTCGGCTTGAAGGCTGCCATGTGGCCGCTTCACCAGTGGCTGCCCAACGCCTAT
TCCTATGGCCCGAGCTTCGTGACGGTGTTCCTGTCGGCGACCGCCACCAAGGTCGCGATCTATGCGATGATCCGGTTCCT
GTTCACGATCTTCCGGCCGGATTACACTTTCGAGCTTGCCAGCTTCTCCTGGATACTTGCCCCGCTCGGCATTGCGGCGA
TGGTGATCTGCAGCTTTCAGGCTGTGTTCCAGACGGATGTTCGGCGCACGCTGGCCTATTCCTCGGTGGCGCAGGTTGGC
TACATGCTGCTCGGTGTATCGCTGGCCACTGCGCCCGGCGTCGCGGCCGGTCTCCTGCATCTCTTCAACCACGCCCTCAT
GAAAGGCGCGCTGTTCATGGCGATCGCCGGCATCGTCATGACCCATAAAGGCACCACGATCCGCGATTTCGCCGGTCTGG
GTCGCACGGCGCCGTGGACCATGACGGCCTTTGCCATCGCGGGCCTCTCACTGATCGGCGTACCGCTGACGGCCGGGTTC
ATGTCAAAGCTTCAGCTCGGTTACGCGCTGTTTGCCGAAGGCTGGTGGTGGGCTGTGCTGCTGGTTGTGTTCTCCAGCTT
CCTGGCCGTGTTCTATGTCGGCCGCATCCTGCAGGCAGCCTTCTTCCAGCCGCCCGATAATCCAAGCAAGATCCACAAGG
AGGCGCCACTTCTCATCCTTGTGCCGCTCTGGATACTTGCGCTCGCCAACCTTTATTTCGGCATCGCCGCTGACTTCCCG
CTGGGACTTGCCCAGGACGGCGCAGTGGCTGCCCTCGGACTGGAGGCGTTCCGATGA

Upstream 100 bases:

>100_bases
TGGAGACCGCGCTCGCTCAGGAACTCGAAGCAGCCAAAGACATCAATGAGGCTTCGGCATGATGCTCGAATCTGTGCTGA
CTTCTGCGCCCGCCTGGGCG

Downstream 100 bases:

>100_bases
ACCCGGAAACCCTCATTGCCTCGATCCTTGTTCTGCCGCTGCTGATTGCGGCAGGCATCGCCGCGACGGGCAAGTTCCCG
AATATTCGTGAGGGCGTGAC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAHAPVLLVAIPMLIAPLVAILPSGRLAWLLAMATTAVSFYLAIVTLGLVQSSPTGILSYEIGRWPPPIGIEFRTDALNA
MILLLVTGIGFLASVFSWPTISSELGNHNKLGMFYAAFLLCLAGLLGVASTGDAFNLFVFLEISSLATYVMVAMGARRDR
RALPAAFNYLIMGTLGASFYIIGIGFLYAATGTLNMADIADRLPALTDSRSVQVGFAFIIVGLGLKAAMWPLHQWLPNAY
SYGPSFVTVFLSATATKVAIYAMIRFLFTIFRPDYTFELASFSWILAPLGIAAMVICSFQAVFQTDVRRTLAYSSVAQVG
YMLLGVSLATAPGVAAGLLHLFNHALMKGALFMAIAGIVMTHKGTTIRDFAGLGRTAPWTMTAFAIAGLSLIGVPLTAGF
MSKLQLGYALFAEGWWWAVLLVVFSSFLAVFYVGRILQAAFFQPPDNPSKIHKEAPLLILVPLWILALANLYFGIAADFP
LGLAQDGAVAALGLEAFR

Sequences:

>Translated_498_residues
MAHAPVLLVAIPMLIAPLVAILPSGRLAWLLAMATTAVSFYLAIVTLGLVQSSPTGILSYEIGRWPPPIGIEFRTDALNA
MILLLVTGIGFLASVFSWPTISSELGNHNKLGMFYAAFLLCLAGLLGVASTGDAFNLFVFLEISSLATYVMVAMGARRDR
RALPAAFNYLIMGTLGASFYIIGIGFLYAATGTLNMADIADRLPALTDSRSVQVGFAFIIVGLGLKAAMWPLHQWLPNAY
SYGPSFVTVFLSATATKVAIYAMIRFLFTIFRPDYTFELASFSWILAPLGIAAMVICSFQAVFQTDVRRTLAYSSVAQVG
YMLLGVSLATAPGVAAGLLHLFNHALMKGALFMAIAGIVMTHKGTTIRDFAGLGRTAPWTMTAFAIAGLSLIGVPLTAGF
MSKLQLGYALFAEGWWWAVLLVVFSSFLAVFYVGRILQAAFFQPPDNPSKIHKEAPLLILVPLWILALANLYFGIAADFP
LGLAQDGAVAALGLEAFR
>Mature_497_residues
AHAPVLLVAIPMLIAPLVAILPSGRLAWLLAMATTAVSFYLAIVTLGLVQSSPTGILSYEIGRWPPPIGIEFRTDALNAM
ILLLVTGIGFLASVFSWPTISSELGNHNKLGMFYAAFLLCLAGLLGVASTGDAFNLFVFLEISSLATYVMVAMGARRDRR
ALPAAFNYLIMGTLGASFYIIGIGFLYAATGTLNMADIADRLPALTDSRSVQVGFAFIIVGLGLKAAMWPLHQWLPNAYS
YGPSFVTVFLSATATKVAIYAMIRFLFTIFRPDYTFELASFSWILAPLGIAAMVICSFQAVFQTDVRRTLAYSSVAQVGY
MLLGVSLATAPGVAAGLLHLFNHALMKGALFMAIAGIVMTHKGTTIRDFAGLGRTAPWTMTAFAIAGLSLIGVPLTAGFM
SKLQLGYALFAEGWWWAVLLVVFSSFLAVFYVGRILQAAFFQPPDNPSKIHKEAPLLILVPLWILALANLYFGIAADFPL
GLAQDGAVAALGLEAFR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=379, Percent_Identity=25.3298153034301, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI145693160, Length=383, Percent_Identity=27.9373368146214, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788831, Length=348, Percent_Identity=27.5862068965517, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788827, Length=369, Percent_Identity=26.2872628726287, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788614, Length=412, Percent_Identity=25.9708737864078, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788613, Length=358, Percent_Identity=24.5810055865922, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788829, Length=419, Percent_Identity=23.8663484486874, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367154, Length=434, Percent_Identity=21.6589861751152, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53384; Mature: 53253

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHAPVLLVAIPMLIAPLVAILPSGRLAWLLAMATTAVSFYLAIVTLGLVQSSPTGILSY
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EIGRWPPPIGIEFRTDALNAMILLLVTGIGFLASVFSWPTISSELGNHNKLGMFYAAFLL
ECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHH
CLAGLLGVASTGDAFNLFVFLEISSLATYVMVAMGARRDRRALPAAFNYLIMGTLGASFY
HHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHH
IIGIGFLYAATGTLNMADIADRLPALTDSRSVQVGFAFIIVGLGLKAAMWPLHQWLPNAY
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCHH
SYGPSFVTVFLSATATKVAIYAMIRFLFTIFRPDYTFELASFSWILAPLGIAAMVICSFQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AVFQTDVRRTLAYSSVAQVGYMLLGVSLATAPGVAAGLLHLFNHALMKGALFMAIAGIVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THKGTTIRDFAGLGRTAPWTMTAFAIAGLSLIGVPLTAGFMSKLQLGYALFAEGWWWAVL
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LVVFSSFLAVFYVGRILQAAFFQPPDNPSKIHKEAPLLILVPLWILALANLYFGIAADFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCC
LGLAQDGAVAALGLEAFR
CCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AHAPVLLVAIPMLIAPLVAILPSGRLAWLLAMATTAVSFYLAIVTLGLVQSSPTGILSY
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EIGRWPPPIGIEFRTDALNAMILLLVTGIGFLASVFSWPTISSELGNHNKLGMFYAAFLL
ECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHH
CLAGLLGVASTGDAFNLFVFLEISSLATYVMVAMGARRDRRALPAAFNYLIMGTLGASFY
HHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHH
IIGIGFLYAATGTLNMADIADRLPALTDSRSVQVGFAFIIVGLGLKAAMWPLHQWLPNAY
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCHH
SYGPSFVTVFLSATATKVAIYAMIRFLFTIFRPDYTFELASFSWILAPLGIAAMVICSFQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AVFQTDVRRTLAYSSVAQVGYMLLGVSLATAPGVAAGLLHLFNHALMKGALFMAIAGIVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THKGTTIRDFAGLGRTAPWTMTAFAIAGLSLIGVPLTAGFMSKLQLGYALFAEGWWWAVL
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LVVFSSFLAVFYVGRILQAAFFQPPDNPSKIHKEAPLLILVPLWILALANLYFGIAADFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCC
LGLAQDGAVAALGLEAFR
CCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA