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The map label for this gene is gltD [H]

Identifier: 114800319

GI number: 114800319

Start: 75961

End: 77406

Strand: Direct

Name: gltD [H]

Synonym: HNE_0092

Alternate gene names: 114800319

Gene position: 75961-77406 (Clockwise)

Preceding gene: 114798702

Following gene: 114798288

Centisome position: 2.05

GC content: 61.96

Gene sequence:

>1446_bases
ATGGCCGAGCGTATGCTGCAGTTTACCAAAGTTTCGCGGGCTATGCCCAAGAAACGGAGCGCAAAAGAGCGCGCGGAAGA
TTTTCGCGAGATTTATGGCGAGTTCTCTCCCGATGCGGCCAAGGCGCAGGCAAGCCGGTGTTCCCAATGCGGCGTGCCGT
TCTGCCAGCAGGGCTGCCCCTTGCAGAACAATATCCCCGACTGGCTGAAGCTGGCCGCTGAAGACCGGCTGGAAGAAGCC
TGGCGCGTGTCCTCGGCCACCAACAACATGCCGGAAATCTGCGGCCGCATCTGCCCGCAGGACCGCCTGTGCGAAGGCAT
CTGCACCATCGAACAATCAGGCCACGGCACCGTGACTATCGGATCGGTCGAAAAGTACATCACGGACACGGCCTGGGCCG
AAGGCTGGATTCAACCGATCAAGCCGCCGCGTGAGCGCACCCAGTCTGCGGGTATCATCGGCGCCGGGCCAGGCGGCCTC
GCCGCCGCTGAGCAACTGCGCCGCAAAGGCTATCAGGTGACTGTTTACGACGCGTATGATCGCGGCGGCGGCCTGCTCAT
GTATGGCATCCCCGGCTTCAAATTGGAGAAGGACGTCGTGGAGCGCCGCATCCATCATCTTGAGGCGTCTGGTATCTCGT
TCCGCTTCAATACGCGCATCGGCGCAGATATGTCGCTCGCGGATCTGCGCGAGCAGCATGATACCGTGCTCATTGCGACC
GGCGTATACGCCGCGAAGGACCTTAAATGTCCCGGCGTTGGCGCAGAAGGCGTGCTTCCTGCGCTGGACTATCTCACCGC
GTCCAACAGGGTCGATCTTGGCGACACGGTGGAAGCATACGACGCCGGCGTGCTCAACGCCGAAGGCAAGCGCGTCGTGG
TCATCGGCGGCGGCGACACGGCGATGGACTGTGTGCGCACGGCTATCCGCCAGGGCGCCAAATCGGTCACATGCCTCTAT
CGCCGGGACCGGGTCAACATGCCGGGCTCACAGCGCGAAGTTCAGAATGCGGAAGAAGAGGGGGTTGTTTTCGAATGGCT
CGCCAACCCCGAAGCCATCATTGATTCCAAAGGCGGCAAGGTCAAAGCTGTTCGTGCCAGCCGCATGAAGCTGGGTGAGC
CCGACGCTTCCGGCCGTCAGTCCCCTGTAAAGACAGGCGAGACATTTGATGTGAAGGCCGACATGGTCATCAAGGCGCTT
GGCTTTGATCCGGAAGATCTCCCCGCATTGTTCAATGAGACTTCGCTCACCGTGAATCGTTGGGGGGCGGTGCGTGTGGA
CTATGCAACCCTGGAGACCAGCCTGCCGGGCGTCTATGCGGCCGGCGATATTGTGCGCGGCGCGTCGCTTGTCGTCTGGG
CGATCAAGGATGGCCGCGACGCGGCCGAAGCGATGCACAAGGCAATGAGGGCCGCTGAGGCGGCCGCAAAAGTGGCGGCG
GAGTAA

Upstream 100 bases:

>100_bases
ACCATATAAGTATCATATTGATATTAGATCGTGTGCGAGGTGCTTGCCGGCATTGAATCTGGGATTATAGGAGGGGCTCG
AGCAATTTTGGGGCAAGCCT

Downstream 100 bases:

>100_bases
GTCAGATGTCGGATTATGTGACCAAGTATGAGCAAAACCGTCAGCGCCTGATCGACGGGCATGCCTACAATCCCGAGGAC
GAACGCGACGCTTGCGGCGT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAERMLQFTKVSRAMPKKRSAKERAEDFREIYGEFSPDAAKAQASRCSQCGVPFCQQGCPLQNNIPDWLKLAAEDRLEEA
WRVSSATNNMPEICGRICPQDRLCEGICTIEQSGHGTVTIGSVEKYITDTAWAEGWIQPIKPPRERTQSAGIIGAGPGGL
AAAEQLRRKGYQVTVYDAYDRGGGLLMYGIPGFKLEKDVVERRIHHLEASGISFRFNTRIGADMSLADLREQHDTVLIAT
GVYAAKDLKCPGVGAEGVLPALDYLTASNRVDLGDTVEAYDAGVLNAEGKRVVVIGGGDTAMDCVRTAIRQGAKSVTCLY
RRDRVNMPGSQREVQNAEEEGVVFEWLANPEAIIDSKGGKVKAVRASRMKLGEPDASGRQSPVKTGETFDVKADMVIKAL
GFDPEDLPALFNETSLTVNRWGAVRVDYATLETSLPGVYAAGDIVRGASLVVWAIKDGRDAAEAMHKAMRAAEAAAKVAA
E

Sequences:

>Translated_481_residues
MAERMLQFTKVSRAMPKKRSAKERAEDFREIYGEFSPDAAKAQASRCSQCGVPFCQQGCPLQNNIPDWLKLAAEDRLEEA
WRVSSATNNMPEICGRICPQDRLCEGICTIEQSGHGTVTIGSVEKYITDTAWAEGWIQPIKPPRERTQSAGIIGAGPGGL
AAAEQLRRKGYQVTVYDAYDRGGGLLMYGIPGFKLEKDVVERRIHHLEASGISFRFNTRIGADMSLADLREQHDTVLIAT
GVYAAKDLKCPGVGAEGVLPALDYLTASNRVDLGDTVEAYDAGVLNAEGKRVVVIGGGDTAMDCVRTAIRQGAKSVTCLY
RRDRVNMPGSQREVQNAEEEGVVFEWLANPEAIIDSKGGKVKAVRASRMKLGEPDASGRQSPVKTGETFDVKADMVIKAL
GFDPEDLPALFNETSLTVNRWGAVRVDYATLETSLPGVYAAGDIVRGASLVVWAIKDGRDAAEAMHKAMRAAEAAAKVAA
E
>Mature_480_residues
AERMLQFTKVSRAMPKKRSAKERAEDFREIYGEFSPDAAKAQASRCSQCGVPFCQQGCPLQNNIPDWLKLAAEDRLEEAW
RVSSATNNMPEICGRICPQDRLCEGICTIEQSGHGTVTIGSVEKYITDTAWAEGWIQPIKPPRERTQSAGIIGAGPGGLA
AAEQLRRKGYQVTVYDAYDRGGGLLMYGIPGFKLEKDVVERRIHHLEASGISFRFNTRIGADMSLADLREQHDTVLIATG
VYAAKDLKCPGVGAEGVLPALDYLTASNRVDLGDTVEAYDAGVLNAEGKRVVVIGGGDTAMDCVRTAIRQGAKSVTCLYR
RDRVNMPGSQREVQNAEEEGVVFEWLANPEAIIDSKGGKVKAVRASRMKLGEPDASGRQSPVKTGETFDVKADMVIKALG
FDPEDLPALFNETSLTVNRWGAVRVDYATLETSLPGVYAAGDIVRGASLVVWAIKDGRDAAEAMHKAMRAAEAAAKVAAE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=450, Percent_Identity=29.1111111111111, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=46.0554371002132, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788811, Length=459, Percent_Identity=45.7516339869281, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI87082180, Length=459, Percent_Identity=43.7908496732026, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788468, Length=443, Percent_Identity=29.7968397291196, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=32.541567695962, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=487, Percent_Identity=35.523613963039, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI71984108, Length=458, Percent_Identity=30.1310043668122, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320030, Length=495, Percent_Identity=34.3434343434343, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28574881, Length=489, Percent_Identity=36.6053169734151, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24665539, Length=489, Percent_Identity=36.6053169734151, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24665547, Length=489, Percent_Identity=36.6053169734151, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24665543, Length=489, Percent_Identity=36.6053169734151, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24640763, Length=462, Percent_Identity=27.7056277056277, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18858217, Length=462, Percent_Identity=27.7056277056277, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52088; Mature: 51956

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERMLQFTKVSRAMPKKRSAKERAEDFREIYGEFSPDAAKAQASRCSQCGVPFCQQGCP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCCC
LQNNIPDWLKLAAEDRLEEAWRVSSATNNMPEICGRICPQDRLCEGICTIEQSGHGTVTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHEEECCCCCEEEE
GSVEKYITDTAWAEGWIQPIKPPRERTQSAGIIGAGPGGLAAAEQLRRKGYQVTVYDAYD
CCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEEEEEEC
RGGGLLMYGIPGFKLEKDVVERRIHHLEASGISFRFNTRIGADMSLADLREQHDTVLIAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCEEEEEE
GVYAAKDLKCPGVGAEGVLPALDYLTASNRVDLGDTVEAYDAGVLNAEGKRVVVIGGGDT
CCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEEECCCCH
AMDCVRTAIRQGAKSVTCLYRRDRVNMPGSQREVQNAEEEGVVFEWLANPEAIIDSKGGK
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCHHEEECCCCE
VKAVRASRMKLGEPDASGRQSPVKTGETFDVKADMVIKALGFDPEDLPALFNETSLTVNR
EEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHCCCCEEEEC
WGAVRVDYATLETSLPGVYAAGDIVRGASLVVWAIKDGRDAAEAMHKAMRAAEAAAKVAA
CCEEEEEEEEHHCCCCCCEECCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
AERMLQFTKVSRAMPKKRSAKERAEDFREIYGEFSPDAAKAQASRCSQCGVPFCQQGCP
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCCC
LQNNIPDWLKLAAEDRLEEAWRVSSATNNMPEICGRICPQDRLCEGICTIEQSGHGTVTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHEEECCCCCEEEE
GSVEKYITDTAWAEGWIQPIKPPRERTQSAGIIGAGPGGLAAAEQLRRKGYQVTVYDAYD
CCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEEEEEEC
RGGGLLMYGIPGFKLEKDVVERRIHHLEASGISFRFNTRIGADMSLADLREQHDTVLIAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCEEEEEE
GVYAAKDLKCPGVGAEGVLPALDYLTASNRVDLGDTVEAYDAGVLNAEGKRVVVIGGGDT
CCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEEECCCCH
AMDCVRTAIRQGAKSVTCLYRRDRVNMPGSQREVQNAEEEGVVFEWLANPEAIIDSKGGK
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCHHEEECCCCE
VKAVRASRMKLGEPDASGRQSPVKTGETFDVKADMVIKALGFDPEDLPALFNETSLTVNR
EEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHCCCCEEEEC
WGAVRVDYATLETSLPGVYAAGDIVRGASLVVWAIKDGRDAAEAMHKAMRAAEAAAKVAA
CCEEEEEEEEHHCCCCCCEECCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]