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The map label for this gene is aroA

Identifier: 114800272

GI number: 114800272

Start: 181563

End: 182882

Strand: Reverse

Name: aroA

Synonym: HNE_0195

Alternate gene names: 114800272

Gene position: 182882-181563 (Counterclockwise)

Preceding gene: 114799341

Following gene: 114799497

Centisome position: 4.94

GC content: 65.91

Gene sequence:

>1320_bases
ATGGTATGGACCAGCCACCCGGTTAAACGCCTCGCCGGCGCCATTCGCGCGCCCGGAGACAAATCCTGCAGCCACCGCGC
GCTCATCTTTGGTGGCCTCGCCGAGGGCGAAAGCCGCTTCAGCGGACTGCTGGAAGGCGACGATGTTCTGCGCACCGGAC
AGGCCATGGAAGCGATGGGCGCAACCGTCACCCGTACAGGTCCGGGCAGCTGGGACGTAACCGGGGTCGGCGCCAAAGGG
CTCTCCTCACCCAAGGGCGTGCTGGACTTTGGAAACTCCGGAACCGGCTCGCGCCTGCTGATGGGCGTCATGGCCGGATA
TGACCTCACAGCGTCCCTGACCGGAGACGCCTCGCTCTGCTCGCGCCCGATGAACCGCGTGCTGAACCCGCTGCGGCAGA
TGGGCCTCAAAGATACAGCGGGCCCCGATGGCAAGCTGCCTTTCACGCTCACCGGCTCGAAAAGTCTCAAGGCCATCCGT
TATGCCCCGCCCCAGGCGAGCGCGCAGGTGAAATCGGCCGTGCTGCTGGCCGGCCTTAATGCTGAGGGCGAAACAGTTGT
CGCCGAAGCCAAAGCCACCCGCGACCATACCGAGCGGATGTTGCAGGGCTTTGGCGCGACACTTTCGTTCAGGATGGCAC
CCGGCGGCGTTCATGAGATCGCCCTGAAGGGTGGCCAGCGCCTGCGCGGGCTGGATGCAGAGATCCCCGGCGACCCGTCT
TCAGCCGCCTTCCTGATCGCCGCAGGGCTCCTCTCGCCGCAAGGCGACGTGCTGGTGGAGGGCGTCATGTCCAACCCCAC
CCGTTCCGGCTTCTATGATGTGGCCGACCTGATGGGCGCCAGCCTTGGCGCTGACGAGCGCGGGGAAGCGGCCGGCGAGC
GCCTGATCGACATTCATTCGGGCTATGCCGGCCTCAAGGGCATCCATGTACCCGAACGCCTGGTTGCTTCCATGATCGAC
GAATTCCCGATCCTGGCCGTGCTGGCCGCCTTTGCGACCGGGGAAACCCGCGTCACCGGCGCCGAAGAATTGCGCGTGAA
GGAAAGTGACCGGATCGGCGCGGTTGTCGCGATGCTCCGCGTCAACGGCGTGGAGGTGGAAGAGACCGAAGATGGCTTCA
CCGTGCAGGGCTGTGGCGGCCGCGTGCCGGGCGGCGGCCTCGTCGAGACCCGCCATGACCACCGCATCGCCATGAGCGCG
CTGGTGATGGGCACAGCCGCGCAGAAGCCCGTCAGCGTCGACGACATCTCCATGATCGACACGAGCTATCCGGAGTTCAT
GAGCCATATGGCGACCCTGGGCGCGGATATTCGGAGCTGA

Upstream 100 bases:

>100_bases
AGCCGATTGGAAGTTGGCGGCGCGCTTGCCATGAACGCCCCGCGCTGTCAAAGCCCCAATTGACATCTGCAACCCGCCTT
CCCGACGAGACCTTTCCCCA

Downstream 100 bases:

>100_bases
CCCTGCCCCGAGGGAAAAATTGACGAACCCGATTCCCGCGACAGGATTGGCGCATGACCTCACTTCATTCTCAAATGACC
CTCACCCGTGGCCCCGCCAT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVWTSHPVKRLAGAIRAPGDKSCSHRALIFGGLAEGESRFSGLLEGDDVLRTGQAMEAMGATVTRTGPGSWDVTGVGAKG
LSSPKGVLDFGNSGTGSRLLMGVMAGYDLTASLTGDASLCSRPMNRVLNPLRQMGLKDTAGPDGKLPFTLTGSKSLKAIR
YAPPQASAQVKSAVLLAGLNAEGETVVAEAKATRDHTERMLQGFGATLSFRMAPGGVHEIALKGGQRLRGLDAEIPGDPS
SAAFLIAAGLLSPQGDVLVEGVMSNPTRSGFYDVADLMGASLGADERGEAAGERLIDIHSGYAGLKGIHVPERLVASMID
EFPILAVLAAFATGETRVTGAEELRVKESDRIGAVVAMLRVNGVEVEETEDGFTVQGCGGRVPGGGLVETRHDHRIAMSA
LVMGTAAQKPVSVDDISMIDTSYPEFMSHMATLGADIRS

Sequences:

>Translated_439_residues
MVWTSHPVKRLAGAIRAPGDKSCSHRALIFGGLAEGESRFSGLLEGDDVLRTGQAMEAMGATVTRTGPGSWDVTGVGAKG
LSSPKGVLDFGNSGTGSRLLMGVMAGYDLTASLTGDASLCSRPMNRVLNPLRQMGLKDTAGPDGKLPFTLTGSKSLKAIR
YAPPQASAQVKSAVLLAGLNAEGETVVAEAKATRDHTERMLQGFGATLSFRMAPGGVHEIALKGGQRLRGLDAEIPGDPS
SAAFLIAAGLLSPQGDVLVEGVMSNPTRSGFYDVADLMGASLGADERGEAAGERLIDIHSGYAGLKGIHVPERLVASMID
EFPILAVLAAFATGETRVTGAEELRVKESDRIGAVVAMLRVNGVEVEETEDGFTVQGCGGRVPGGGLVETRHDHRIAMSA
LVMGTAAQKPVSVDDISMIDTSYPEFMSHMATLGADIRS
>Mature_439_residues
MVWTSHPVKRLAGAIRAPGDKSCSHRALIFGGLAEGESRFSGLLEGDDVLRTGQAMEAMGATVTRTGPGSWDVTGVGAKG
LSSPKGVLDFGNSGTGSRLLMGVMAGYDLTASLTGDASLCSRPMNRVLNPLRQMGLKDTAGPDGKLPFTLTGSKSLKAIR
YAPPQASAQVKSAVLLAGLNAEGETVVAEAKATRDHTERMLQGFGATLSFRMAPGGVHEIALKGGQRLRGLDAEIPGDPS
SAAFLIAAGLLSPQGDVLVEGVMSNPTRSGFYDVADLMGASLGADERGEAAGERLIDIHSGYAGLKGIHVPERLVASMID
EFPILAVLAAFATGETRVTGAEELRVKESDRIGAVVAMLRVNGVEVEETEDGFTVQGCGGRVPGGGLVETRHDHRIAMSA
LVMGTAAQKPVSVDDISMIDTSYPEFMSHMATLGADIRS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=448, Percent_Identity=28.125, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320332, Length=415, Percent_Identity=28.9156626506024, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_HYPNA (Q0C5R4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758929.1
- ProteinModelPortal:   Q0C5R4
- SMR:   Q0C5R4
- STRING:   Q0C5R4
- GeneID:   4287507
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0195
- NMPDR:   fig|228405.5.peg.193
- TIGR:   HNE_0195
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   HNEP81032:HNE_0195-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45601; Mature: 45601

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWTSHPVKRLAGAIRAPGDKSCSHRALIFGGLAEGESRFSGLLEGDDVLRTGQAMEAMG
CCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHCHHHHHHC
ATVTRTGPGSWDVTGVGAKGLSSPKGVLDFGNSGTGSRLLMGVMAGYDLTASLTGDASLC
CEEEECCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHH
SRPMNRVLNPLRQMGLKDTAGPDGKLPFTLTGSKSLKAIRYAPPQASAQVKSAVLLAGLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHEEEEEECC
AEGETVVAEAKATRDHTERMLQGFGATLSFRMAPGGVHEIALKGGQRLRGLDAEIPGDPS
CCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCC
SAAFLIAAGLLSPQGDVLVEGVMSNPTRSGFYDVADLMGASLGADERGEAAGERLIDIHS
CHHHHHHHHCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCEEEEEC
GYAGLKGIHVPERLVASMIDEFPILAVLAAFATGETRVTGAEELRVKESDRIGAVVAMLR
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEECCHHHHEECCCCHHHHHHHHHH
VNGVEVEETEDGFTVQGCGGRVPGGGLVETRHDHRIAMSALVMGTAAQKPVSVDDISMID
HCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHC
TSYPEFMSHMATLGADIRS
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVWTSHPVKRLAGAIRAPGDKSCSHRALIFGGLAEGESRFSGLLEGDDVLRTGQAMEAMG
CCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHCHHHHHHC
ATVTRTGPGSWDVTGVGAKGLSSPKGVLDFGNSGTGSRLLMGVMAGYDLTASLTGDASLC
CEEEECCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHH
SRPMNRVLNPLRQMGLKDTAGPDGKLPFTLTGSKSLKAIRYAPPQASAQVKSAVLLAGLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHEEEEEECC
AEGETVVAEAKATRDHTERMLQGFGATLSFRMAPGGVHEIALKGGQRLRGLDAEIPGDPS
CCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCC
SAAFLIAAGLLSPQGDVLVEGVMSNPTRSGFYDVADLMGASLGADERGEAAGERLIDIHS
CHHHHHHHHCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCEEEEEC
GYAGLKGIHVPERLVASMIDEFPILAVLAAFATGETRVTGAEELRVKESDRIGAVVAMLR
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEECCHHHHEECCCCHHHHHHHHHH
VNGVEVEETEDGFTVQGCGGRVPGGGLVETRHDHRIAMSALVMGTAAQKPVSVDDISMID
HCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHC
TSYPEFMSHMATLGADIRS
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA