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The map label for this gene is rsuA [H]

Identifier: 114800237

GI number: 114800237

Start: 762551

End: 763225

Strand: Reverse

Name: rsuA [H]

Synonym: HNE_0755

Alternate gene names: 114800237

Gene position: 763225-762551 (Counterclockwise)

Preceding gene: 114797404

Following gene: 114799256

Centisome position: 20.6

GC content: 63.11

Gene sequence:

>675_bases
ATGACCGAAACCCGCAAGCTCGCAAAATACCTCGCCAGTCTTGGCTATGGTTCGCGCAAGGAGATGGAAGTGGCCATCCG
GCGCGGCCGCGTGACCGGCTGGGGCGAAGACGTGGAGTTTGACGGCGAACCGCTGGACCCAGGCCCAGGCATGGTGATCC
TGATGCACAAGCCGGCGGGCTATACCTGCTCCCATCAGGATCAGGGGAAGCTGGTCTATGATCTTCTCCCCCCGCGCTTC
CTGAAACGTGACCCGAAGATTTCGTCTGTTGGCCGCCTCGACGCGGACACGACCGGACTTCTTCTGTTTACGGATGATGG
CGCCCTGCTCCACCGGCTGATCTCGCCCAAGTCCGACATTCCCAAAACCTATGAAGCCACCCTCGCGCGCCCGCTGGAGG
GGTTTGAAAAGGAATTTTTCGCCTCAGGATCGCTGATCCTGCGCGGGGAAGACAAGCCCTGCCTGCCTGCCGAGTTGGAA
GTGACGGGCGAACGGACGGCGCGTCTTACGCTGAGCGAAGGGCGGTATCATCAGGTGCGCCGGATGTTTGCCGCCGCTGG
CAACCATGTCGAAGCCCTTCACCGCAGCCGGTTTGGCCCGCTGGAGCTGGGCGATCTGTCGGAAGGCGCGTGGCGCCTGC
TGACAGAGGCCGAACGTGCGGGGCTTGCCCCCTAG

Upstream 100 bases:

>100_bases
GAACTCTCTGCCTGCTTCCGCGGCGTTGAGATGCTTGATGACGAAGACGGCTACAAGATCATCAAGGCCGAGAAGCCCAA
GCGCCAGAAATGAGCGGGCA

Downstream 100 bases:

>100_bases
CGTCCACGCGCGTCAGCGGCTATCTCCCCGGCCATGGCCCAGCCCCCACTGATTACCCTCACCGATGTCCGCCTTTCTTT
CGGCAATACGCCCCTGTTCG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTETRKLAKYLASLGYGSRKEMEVAIRRGRVTGWGEDVEFDGEPLDPGPGMVILMHKPAGYTCSHQDQGKLVYDLLPPRF
LKRDPKISSVGRLDADTTGLLLFTDDGALLHRLISPKSDIPKTYEATLARPLEGFEKEFFASGSLILRGEDKPCLPAELE
VTGERTARLTLSEGRYHQVRRMFAAAGNHVEALHRSRFGPLELGDLSEGAWRLLTEAERAGLAP

Sequences:

>Translated_224_residues
MTETRKLAKYLASLGYGSRKEMEVAIRRGRVTGWGEDVEFDGEPLDPGPGMVILMHKPAGYTCSHQDQGKLVYDLLPPRF
LKRDPKISSVGRLDADTTGLLLFTDDGALLHRLISPKSDIPKTYEATLARPLEGFEKEFFASGSLILRGEDKPCLPAELE
VTGERTARLTLSEGRYHQVRRMFAAAGNHVEALHRSRFGPLELGDLSEGAWRLLTEAERAGLAP
>Mature_223_residues
TETRKLAKYLASLGYGSRKEMEVAIRRGRVTGWGEDVEFDGEPLDPGPGMVILMHKPAGYTCSHQDQGKLVYDLLPPRFL
KRDPKISSVGRLDADTTGLLLFTDDGALLHRLISPKSDIPKTYEATLARPLEGFEKEFFASGSLILRGEDKPCLPAELEV
TGERTARLTLSEGRYHQVRRMFAAAGNHVEALHRSRFGPLELGDLSEGAWRLLTEAERAGLAP

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=42.5531914893617, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=31.404958677686, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=26.4069264069264, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081838, Length=189, Percent_Identity=29.6296296296296, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 24804; Mature: 24672

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETRKLAKYLASLGYGSRKEMEVAIRRGRVTGWGEDVEFDGEPLDPGPGMVILMHKPAG
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
YTCSHQDQGKLVYDLLPPRFLKRDPKISSVGRLDADTTGLLLFTDDGALLHRLISPKSDI
CCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCC
PKTYEATLARPLEGFEKEFFASGSLILRGEDKPCLPAELEVTGERTARLTLSEGRYHQVR
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHH
RMFAAAGNHVEALHRSRFGPLELGDLSEGAWRLLTEAERAGLAP
HHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TETRKLAKYLASLGYGSRKEMEVAIRRGRVTGWGEDVEFDGEPLDPGPGMVILMHKPAG
CHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
YTCSHQDQGKLVYDLLPPRFLKRDPKISSVGRLDADTTGLLLFTDDGALLHRLISPKSDI
CCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCC
PKTYEATLARPLEGFEKEFFASGSLILRGEDKPCLPAELEVTGERTARLTLSEGRYHQVR
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHH
RMFAAAGNHVEALHRSRFGPLELGDLSEGAWRLLTEAERAGLAP
HHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]