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The map label for this gene is aspS

Identifier: 114800235

GI number: 114800235

Start: 2358217

End: 2360046

Strand: Reverse

Name: aspS

Synonym: HNE_2257

Alternate gene names: 114800235

Gene position: 2360046-2358217 (Counterclockwise)

Preceding gene: 114800396

Following gene: 114798194

Centisome position: 63.7

GC content: 62.95

Gene sequence:

>1830_bases
ATGCATGCCTATCGCACCCATACCTGCGGCGAGCTGCGCAAATCCCATGTGGGAGAAACCGTAAAGATTTCAGGCTGGCT
GCACCGACGCCGCGATCATGGCGGGGTGATGTTCATCGACCTGCGCGACCATTATGGCCTCACCCAGATCGTGTTCAATC
CGGGCTCGCCAGGCTTTGCCACGGTTGAGCGCCTGCGCGCCGAAAGCGTCATAACGGTCGAGGGCAAGGTTGTTGCCCGC
GACGCCGAGCTCGTGAATGCGAATCTCGGCACAGGCGAGATCGAAGTCGTCGGCGGGGCGGTTTCCGTCCAGTCCGAGGC
GGCCGAGCTGCCGATGCCGGTGTTCGGCGATCACTCCTATCCTGAGGAAATCCGCCTCAAGCATCGCTATCTGGACCTGC
GCCGCGAGCAGCTGCACAAGAACATGGTTCTGCGCAGCCAGGTCATCGCGAGCCTGCGCCGCCGCATGGTGGCTGAAGGC
TTCACCGAGTACCAGACGCCGATCCTGACCGCCTCCAGCCCGGAAGGCGCGCGCGACTTTCTCGTGCCCAGCCGCCTGCA
CCCCGGCGAGTTCTACGCCCTGCCGCAGGCGCCCCAGCAGTTCAAACAGCTGCTGATGGTGTCGGGCTTTGACCGCTATT
TCCAGATCGCGCCCTGCTTCCGCGATGAAGACGCCCGCGCCGACCGCTCGCCTGGTGAATTCTACCAGCTCGATATCGAG
ATGAGCTTCGTCACGCAGGAAGACGTGTTCAATTCGATCGAGCCCGTCATGCGCGGTGTGTTCGAGGAGTTTGCAGACTG
GGAAGGCAAGGGCCGCAAGGTCGCCGAAGGCCCGTTCGTGCACATCCCTTACGCCGAAGCGATGCTGAAATACGGCGTCG
ACAAGCCTGACCTGCGCAACCCGATCCTCTGCTCGGATGTCTCCGACATCATGATGCGGGACGACGTGACGCTCGCCGTG
TTCAAGAAGATTGTCGGCGGCGGCGGCATCGTGCGCGCCATTCCCGCGCCGAACTCCGGCGACCAGCCGCGCTCGTTCTT
CGACAAGATGGACGCGTGGGCGAAGAAGGAAATGCAGGCGCCCGGCCTCGGCTATATGCGCTTTGTGGAAGAGAACGGCG
CGCTGACGGCCACCGGCCCCATTGCGAAATTCTTCGAGCCGGCCGCTCTGGCCGCTCTGGCCGAAAAAGCCGGCGTGAAG
GCGGGCGACGCCCTCTTCTTCTCCGCTGGTGACAAGACCGCCGCAGTCAGGCTGCAAGGCCAGGCGCGTATTCGCGTGGG
CGAGGAACTCGGCATCATCGAGCCGGGCGTCTTCCGCTTCTGCTGGATTGTCGATTTCCCGATGTATGAGTGGGACGAAG
ACAACAAGAAGGTCGACTTCAGCCACAACCCGTTCTCGATGCCCCAGGGCGGCATGGAAGCGCTTCTGGCTGCCGATACG
GTTGAGAAACAGCTGGATCTCAAGGCCTTCCAGTACGACATCGTCTGTAACGGCGTGGAGCTTTCCTCCGGCGCCATCCG
GAACCACCGCCCGGAAGTGATGCTGAAAGCCTTCGAACTTGCTGGCTACGGCCCGGAAGTGGTTGAGGCGAAGTTTGGTG
GCATGCTCAACGCATTCCGCTATGGCGCCCCGCCGCATGGCGGCATCGCTCCAGGCGTTGACCGGATCGTCATGCTGTTG
GCCGAAACCGACTCGCTGCGCGACGTGATCCTCTTCCCGATGAACCAGCAGGCGCGTGATCTGTTGATGGGCGCGCCGAG
CCCGGTGGATGAACGCCAGCTGAAAGAGCTGAACATCCGCCTTGCGCCCGTGGTCAAGCCGCAGCCCTGA

Upstream 100 bases:

>100_bases
ATCCCCTGAGCGCTTATCGCACCAACTTGACAAAGGCGAGGGGGTCTGTGCCCTACGCCGCTTCTTTCTGACTCAAGCGA
TTTTCCGGAGAATTCGCCCG

Downstream 100 bases:

>100_bases
GGCTTGCCCGCACACCAAAAACGAACCGGCGGGTGATCAGCCCGGCGGTTTTGTTTTGTGGAGAATTCAGCGGCCGCTCA
GTCTTTGCCGATGAAATCCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MHAYRTHTCGELRKSHVGETVKISGWLHRRRDHGGVMFIDLRDHYGLTQIVFNPGSPGFATVERLRAESVITVEGKVVAR
DAELVNANLGTGEIEVVGGAVSVQSEAAELPMPVFGDHSYPEEIRLKHRYLDLRREQLHKNMVLRSQVIASLRRRMVAEG
FTEYQTPILTASSPEGARDFLVPSRLHPGEFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFNSIEPVMRGVFEEFADWEGKGRKVAEGPFVHIPYAEAMLKYGVDKPDLRNPILCSDVSDIMMRDDVTLAV
FKKIVGGGGIVRAIPAPNSGDQPRSFFDKMDAWAKKEMQAPGLGYMRFVEENGALTATGPIAKFFEPAALAALAEKAGVK
AGDALFFSAGDKTAAVRLQGQARIRVGEELGIIEPGVFRFCWIVDFPMYEWDEDNKKVDFSHNPFSMPQGGMEALLAADT
VEKQLDLKAFQYDIVCNGVELSSGAIRNHRPEVMLKAFELAGYGPEVVEAKFGGMLNAFRYGAPPHGGIAPGVDRIVMLL
AETDSLRDVILFPMNQQARDLLMGAPSPVDERQLKELNIRLAPVVKPQP

Sequences:

>Translated_609_residues
MHAYRTHTCGELRKSHVGETVKISGWLHRRRDHGGVMFIDLRDHYGLTQIVFNPGSPGFATVERLRAESVITVEGKVVAR
DAELVNANLGTGEIEVVGGAVSVQSEAAELPMPVFGDHSYPEEIRLKHRYLDLRREQLHKNMVLRSQVIASLRRRMVAEG
FTEYQTPILTASSPEGARDFLVPSRLHPGEFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFNSIEPVMRGVFEEFADWEGKGRKVAEGPFVHIPYAEAMLKYGVDKPDLRNPILCSDVSDIMMRDDVTLAV
FKKIVGGGGIVRAIPAPNSGDQPRSFFDKMDAWAKKEMQAPGLGYMRFVEENGALTATGPIAKFFEPAALAALAEKAGVK
AGDALFFSAGDKTAAVRLQGQARIRVGEELGIIEPGVFRFCWIVDFPMYEWDEDNKKVDFSHNPFSMPQGGMEALLAADT
VEKQLDLKAFQYDIVCNGVELSSGAIRNHRPEVMLKAFELAGYGPEVVEAKFGGMLNAFRYGAPPHGGIAPGVDRIVMLL
AETDSLRDVILFPMNQQARDLLMGAPSPVDERQLKELNIRLAPVVKPQP
>Mature_609_residues
MHAYRTHTCGELRKSHVGETVKISGWLHRRRDHGGVMFIDLRDHYGLTQIVFNPGSPGFATVERLRAESVITVEGKVVAR
DAELVNANLGTGEIEVVGGAVSVQSEAAELPMPVFGDHSYPEEIRLKHRYLDLRREQLHKNMVLRSQVIASLRRRMVAEG
FTEYQTPILTASSPEGARDFLVPSRLHPGEFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
MSFVTQEDVFNSIEPVMRGVFEEFADWEGKGRKVAEGPFVHIPYAEAMLKYGVDKPDLRNPILCSDVSDIMMRDDVTLAV
FKKIVGGGGIVRAIPAPNSGDQPRSFFDKMDAWAKKEMQAPGLGYMRFVEENGALTATGPIAKFFEPAALAALAEKAGVK
AGDALFFSAGDKTAAVRLQGQARIRVGEELGIIEPGVFRFCWIVDFPMYEWDEDNKKVDFSHNPFSMPQGGMEALLAADT
VEKQLDLKAFQYDIVCNGVELSSGAIRNHRPEVMLKAFELAGYGPEVVEAKFGGMLNAFRYGAPPHGGIAPGVDRIVMLL
AETDSLRDVILFPMNQQARDLLMGAPSPVDERQLKELNIRLAPVVKPQP

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=610, Percent_Identity=35.7377049180328, Blast_Score=349, Evalue=5e-96,
Organism=Homo sapiens, GI45439306, Length=322, Percent_Identity=26.3975155279503, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI226491502, Length=254, Percent_Identity=25.9842519685039, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI5031815, Length=254, Percent_Identity=26.3779527559055, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI194272210, Length=254, Percent_Identity=26.3779527559055, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1788173, Length=600, Percent_Identity=40.1666666666667, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=27.2108843537415, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789256, Length=320, Percent_Identity=26.875, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787161, Length=284, Percent_Identity=25.7042253521127, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=612, Percent_Identity=34.3137254901961, Blast_Score=308, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI71984122, Length=308, Percent_Identity=25.3246753246753, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=253, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=277, Percent_Identity=25.6317689530686, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=318, Percent_Identity=34.9056603773585, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321807, Length=332, Percent_Identity=26.2048192771084, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=259, Percent_Identity=26.6409266409266, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=35.3820598006645, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17136276, Length=269, Percent_Identity=26.3940520446097, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921528, Length=237, Percent_Identity=27.0042194092827, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_HYPNA (Q0BZZ1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760952.1
- ProteinModelPortal:   Q0BZZ1
- SMR:   Q0BZZ1
- STRING:   Q0BZZ1
- GeneID:   4287470
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2257
- NMPDR:   fig|228405.5.peg.2175
- TIGR:   HNE_2257
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   HNEP81032:HNE_2257-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67649; Mature: 67649

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYRTHTCGELRKSHVGETVKISGWLHRRRDHGGVMFIDLRDHYGLTQIVFNPGSPGFA
CCCCCCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCHH
TVERLRAESVITVEGKVVARDAELVNANLGTGEIEVVGGAVSVQSEAAELPMPVFGDHSY
HHHHHHCCCEEEECCEEEEECHHEEECCCCCCEEEEEECEEEECCHHHHCCCCCCCCCCC
PEEIRLKHRYLDLRREQLHKNMVLRSQVIASLRRRMVAEGFTEYQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHC
LVPSRLHPGEFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFNSIEPVMRGVFEEFADWEGKGRKVAEGPFVHIPYAEAMLKYGVDKPDLRN
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCHHHHHHHHCCCCCCCCC
PILCSDVSDIMMRDDVTLAVFKKIVGGGGIVRAIPAPNSGDQPRSFFDKMDAWAKKEMQA
CEEECCHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
PGLGYMRFVEENGALTATGPIAKFFEPAALAALAEKAGVKAGDALFFSAGDKTAAVRLQG
CCCCEEEEEECCCCEEECCCHHHHHCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEC
QARIRVGEELGIIEPGVFRFCWIVDFPMYEWDEDNKKVDFSHNPFSMPQGGMEALLAADT
CEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
VEKQLDLKAFQYDIVCNGVELSSGAIRNHRPEVMLKAFELAGYGPEVVEAKFGGMLNAFR
HHHHHCHHHEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
YGAPPHGGIAPGVDRIVMLLAETDSLRDVILFPMNQQARDLLMGAPSPVDERQLKELNIR
CCCCCCCCCCCCHHHHEEHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCEE
LAPVVKPQP
EEECCCCCC
>Mature Secondary Structure
MHAYRTHTCGELRKSHVGETVKISGWLHRRRDHGGVMFIDLRDHYGLTQIVFNPGSPGFA
CCCCCCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCHH
TVERLRAESVITVEGKVVARDAELVNANLGTGEIEVVGGAVSVQSEAAELPMPVFGDHSY
HHHHHHCCCEEEECCEEEEECHHEEECCCCCCEEEEEECEEEECCHHHHCCCCCCCCCCC
PEEIRLKHRYLDLRREQLHKNMVLRSQVIASLRRRMVAEGFTEYQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHC
LVPSRLHPGEFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDIE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFNSIEPVMRGVFEEFADWEGKGRKVAEGPFVHIPYAEAMLKYGVDKPDLRN
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCHHHHHHHHCCCCCCCCC
PILCSDVSDIMMRDDVTLAVFKKIVGGGGIVRAIPAPNSGDQPRSFFDKMDAWAKKEMQA
CEEECCHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
PGLGYMRFVEENGALTATGPIAKFFEPAALAALAEKAGVKAGDALFFSAGDKTAAVRLQG
CCCCEEEEEECCCCEEECCCHHHHHCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEC
QARIRVGEELGIIEPGVFRFCWIVDFPMYEWDEDNKKVDFSHNPFSMPQGGMEALLAADT
CEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
VEKQLDLKAFQYDIVCNGVELSSGAIRNHRPEVMLKAFELAGYGPEVVEAKFGGMLNAFR
HHHHHCHHHEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
YGAPPHGGIAPGVDRIVMLLAETDSLRDVILFPMNQQARDLLMGAPSPVDERQLKELNIR
CCCCCCCCCCCCHHHHEEHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCEE
LAPVVKPQP
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA