Click here to switch to the map view.

The map label for this gene is leuC1 [H]

Identifier: 114800168

GI number: 114800168

Start: 39148

End: 40545

Strand: Direct

Name: leuC1 [H]

Synonym: HNE_0047

Alternate gene names: 114800168

Gene position: 39148-40545 (Clockwise)

Preceding gene: 114797917

Following gene: 114800485

Centisome position: 1.06

GC content: 63.3

Gene sequence:

>1398_bases
ATGTCCGCAAAGACACTCTACGACAAGATCTGGGATTCCCATGTCGTTCACACCGATGCGCAGTCAGGTGAGAGCCTTCT
TTATATCGATCTCCACCTCATTCATGAGGTGACGACGCCTCAGGCTTTTGCGGGCCTGAAAGCCGCAGGCCGCAAGGTGC
GCCGGCCTGAACTGACGCTCGCGGTCGCCGATCACAACACGCCGACAGAAAACCAGGCGCTCGGGCTGGCGGGCGTGAAG
GATGCCGAGGCGCGCAACCAGCTCGAGACGCTGACCCGCAATGTCGCCGAGTACGGCATCGAGTTCTTCCAGATGGGCGA
CATCAATAACGGTATCGTCCATGTCGTCGGCCCGGAGCAGGGGCGCACCCAGCCGGGCATGACGATCGTATGCGGCGACA
GCCATACCTCAACCCACGGCGCGTTCGGCGCGCTGGCGCATGGCATCGGCACCTCTGAAGTCGAGCATGTGCTCGCCACG
CAGACACTGCGCGCGCGCAAAATGAAGAATATGGCGGTGGAAGTGTCCGGCCGTTTGCGCGACGGGGTCACCGCCAAGGA
TCTCGCGCTGCATGTCATCGCCATCACCGGGACCGCCGGTGGTACGGGCCATGTGATCGAGTATCGCGGCGAAGCGGTCC
GCGCGCTCTCCATGGAAGGGCGCATGACGCTCTGCAATCTCTCGATCGAAGGCGGGGCCCGCGCAGGTCTTGTTGCGCCA
GACGATGTTACGTTCGAGTACATGAAGGGCCGCCCGTCCACGCCCAAGGGCGGCGCCTGGGAGCTGGCCGAGAAACACTG
GCGCACGCTGTATTCAGACGATGATGCTGTCTTCGATGAGGTGCTGCGCATCAAGGGCGAAGACGTTGAGCCGACCGTCA
CCTGGGGCACCAGCCCGGAACAGGGCATCGCGATTTCCGGCATCATTCCGAAACCCGAGGATTTCTCCGACCCGGTCAAG
CGCGCCGCGTGCGAGCGCGCTTTGGACTATATGGGCCTTGAGGGCGGCAAGCCGATCGCGGGCACGCCCGTTCAGCGCGT
TTTCATCGGCTCGTGCACCAACAGCCGGATCGAGGATCTGCGCGCCGCCGCTGAAGTGGCCAAAGGCAACAAGGTGGCAG
ACGGCGTGCGGGCCATGGTTGTGCCGGGTTCCGGCCTCGTTCGGGCGCAAGCCGAAGCCGAAGGCCTCGATCTTGTTCTC
ATGGAAGCTGGCTTTGAATGGCGCGAGCCAGGCTGCTCCATGTGCCTTGGCATGAACCCGGATATTCTTTCGCCAGGCGA
ACGGTGCGCCTCCACATCAAATCGCAATTTCGAGGGTCGGCAGGGCAGGGGTGGGCGTACCCATCTGATGAGCCCTGCTC
TGGCCGCCCGTGCCGCCATCAATGGCGTGCTCGGATAA

Upstream 100 bases:

>100_bases
TATGTGAAACGCCCTCCCTTCCGGAGGGCGTTTTCGCATGGGCAAGGCTTCCCTATGTGAGCTTCAGCGCTATTGTGCGC
CGCGAAAGTGAGAGGCCGAA

Downstream 100 bases:

>100_bases
TCTGTTAACTGATCAGGATCATTTCATGACGTCTTCTGACCTCACCCCCCGCCAGAACTGGACCCGCGAAGAGATTGCGG
CAATCTATGATCTGCCCCTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSAKTLYDKIWDSHVVHTDAQSGESLLYIDLHLIHEVTTPQAFAGLKAAGRKVRRPELTLAVADHNTPTENQALGLAGVK
DAEARNQLETLTRNVAEYGIEFFQMGDINNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QTLRARKMKNMAVEVSGRLRDGVTAKDLALHVIAITGTAGGTGHVIEYRGEAVRALSMEGRMTLCNLSIEGGARAGLVAP
DDVTFEYMKGRPSTPKGGAWELAEKHWRTLYSDDDAVFDEVLRIKGEDVEPTVTWGTSPEQGIAISGIIPKPEDFSDPVK
RAACERALDYMGLEGGKPIAGTPVQRVFIGSCTNSRIEDLRAAAEVAKGNKVADGVRAMVVPGSGLVRAQAEAEGLDLVL
MEAGFEWREPGCSMCLGMNPDILSPGERCASTSNRNFEGRQGRGGRTHLMSPALAARAAINGVLG

Sequences:

>Translated_465_residues
MSAKTLYDKIWDSHVVHTDAQSGESLLYIDLHLIHEVTTPQAFAGLKAAGRKVRRPELTLAVADHNTPTENQALGLAGVK
DAEARNQLETLTRNVAEYGIEFFQMGDINNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QTLRARKMKNMAVEVSGRLRDGVTAKDLALHVIAITGTAGGTGHVIEYRGEAVRALSMEGRMTLCNLSIEGGARAGLVAP
DDVTFEYMKGRPSTPKGGAWELAEKHWRTLYSDDDAVFDEVLRIKGEDVEPTVTWGTSPEQGIAISGIIPKPEDFSDPVK
RAACERALDYMGLEGGKPIAGTPVQRVFIGSCTNSRIEDLRAAAEVAKGNKVADGVRAMVVPGSGLVRAQAEAEGLDLVL
MEAGFEWREPGCSMCLGMNPDILSPGERCASTSNRNFEGRQGRGGRTHLMSPALAARAAINGVLG
>Mature_464_residues
SAKTLYDKIWDSHVVHTDAQSGESLLYIDLHLIHEVTTPQAFAGLKAAGRKVRRPELTLAVADHNTPTENQALGLAGVKD
AEARNQLETLTRNVAEYGIEFFQMGDINNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
TLRARKMKNMAVEVSGRLRDGVTAKDLALHVIAITGTAGGTGHVIEYRGEAVRALSMEGRMTLCNLSIEGGARAGLVAPD
DVTFEYMKGRPSTPKGGAWELAEKHWRTLYSDDDAVFDEVLRIKGEDVEPTVTWGTSPEQGIAISGIIPKPEDFSDPVKR
AACERALDYMGLEGGKPIAGTPVQRVFIGSCTNSRIEDLRAAAEVAKGNKVADGVRAMVVPGSGLVRAQAEAEGLDLVLM
EAGFEWREPGCSMCLGMNPDILSPGERCASTSNRNFEGRQGRGGRTHLMSPALAARAAINGVLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=378, Percent_Identity=27.5132275132275, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=25.6756756756757, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786259, Length=460, Percent_Identity=62.1739130434783, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=30.1939058171745, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=28.5714285714286, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=378, Percent_Identity=28.8359788359788, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=28.8524590163934, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=26.5350877192982, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=453, Percent_Identity=60.485651214128, Blast_Score=582, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6323335, Length=476, Percent_Identity=28.3613445378151, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=463, Percent_Identity=25.7019438444924, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=483, Percent_Identity=26.2939958592132, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=478, Percent_Identity=28.6610878661088, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=478, Percent_Identity=28.6610878661088, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=399, Percent_Identity=29.5739348370927, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=27.4633123689727, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645686, Length=457, Percent_Identity=26.4770240700219, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=25.8760107816712, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49581; Mature: 49450

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKTLYDKIWDSHVVHTDAQSGESLLYIDLHLIHEVTTPQAFAGLKAAGRKVRRPELTL
CCCHHHHHHHCCCCEEECCCCCCCEEEEEEEEEEHHHCCCHHHHHHHHHCCCCCCCEEEE
AVADHNTPTENQALGLAGVKDAEARNQLETLTRNVAEYGIEFFQMGDINNGIVHVVGPEQ
EEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCC
GRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRARKMKNMAVEVSGRLR
CCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
DGVTAKDLALHVIAITGTAGGTGHVIEYRGEAVRALSMEGRMTLCNLSIEGGARAGLVAP
CCCCHHHEEEEEEEEEECCCCCCCEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCC
DDVTFEYMKGRPSTPKGGAWELAEKHWRTLYSDDDAVFDEVLRIKGEDVEPTVTWGTSPE
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
QGIAISGIIPKPEDFSDPVKRAACERALDYMGLEGGKPIAGTPVQRVFIGSCTNSRIEDL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEECCCCHHHHHH
RAAAEVAKGNKVADGVRAMVVPGSGLVRAQAEAEGLDLVLMEAGFEWREPGCSMCLGMNP
HHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCCCEEECCCC
DILSPGERCASTSNRNFEGRQGRGGRTHLMSPALAARAAINGVLG
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAKTLYDKIWDSHVVHTDAQSGESLLYIDLHLIHEVTTPQAFAGLKAAGRKVRRPELTL
CCHHHHHHHCCCCEEECCCCCCCEEEEEEEEEEHHHCCCHHHHHHHHHCCCCCCCEEEE
AVADHNTPTENQALGLAGVKDAEARNQLETLTRNVAEYGIEFFQMGDINNGIVHVVGPEQ
EEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCC
GRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRARKMKNMAVEVSGRLR
CCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
DGVTAKDLALHVIAITGTAGGTGHVIEYRGEAVRALSMEGRMTLCNLSIEGGARAGLVAP
CCCCHHHEEEEEEEEEECCCCCCCEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCC
DDVTFEYMKGRPSTPKGGAWELAEKHWRTLYSDDDAVFDEVLRIKGEDVEPTVTWGTSPE
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
QGIAISGIIPKPEDFSDPVKRAACERALDYMGLEGGKPIAGTPVQRVFIGSCTNSRIEDL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEECCCCHHHHHH
RAAAEVAKGNKVADGVRAMVVPGSGLVRAQAEAEGLDLVLMEAGFEWREPGCSMCLGMNP
HHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCCCEEECCCC
DILSPGERCASTSNRNFEGRQGRGGRTHLMSPALAARAAINGVLG
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA