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The map label for this gene is bioB

Identifier: 114800485

GI number: 114800485

Start: 40571

End: 41533

Strand: Direct

Name: bioB

Synonym: HNE_0048

Alternate gene names: 114800485

Gene position: 40571-41533 (Clockwise)

Preceding gene: 114800168

Following gene: 114799333

Centisome position: 1.1

GC content: 61.99

Gene sequence:

>963_bases
ATGACGTCTTCTGACCTCACCCCCCGCCAGAACTGGACCCGCGAAGAGATTGCGGCAATCTATGATCTGCCCCTCGATCA
GCTTTTCGCCCGCGCGCTCGCCGTGAAGGCAGCCCATTGGGCAGACGGCGCCGTGCAAAAGTCGCAGCTCCTGTCGATCA
AGACCGGCGGCTGCGCCGAGAATTGCGGTTATTGCAGCCAGTCAGCGAGCTTCAAGACAGGCCTTAAGGCTGAGAAGCTG
CTCCCGGTTGAAGAAGTTGTTGATGCTGCTCGCCGCGCAAAGGCCAACGGCGCGGACCGTTTCTGCATGGGCGCCGCCTG
GCGCTCGCTGAAAGATCGCGACCTGCCAAAAGTGGCAGAGATGATCTCGGCTGTGAAGGCAGAAGGCCTTGAGACCTGCG
TCACGCTCGGCATGCTTGAAGAAGGCCAGGCTGAAGCGCTGAAAGAAGCGGGCCTCGACTATTACAATCACAACCTCGAC
ACCTCGCGCGAGTATTACAGCACGGTCGTCACGACACGGACGTTTGACGACCGGATCGAAACGCTCGGCCGTGCGCGACA
GGCCGGCATCAAGCTCTGCTGTGGCGGCATCCTCGGCATGGGCGAGTCGCGCTCTGACCGGGTAGGCCTCATCCATGAGC
TTTCCCTGCTTAGCCCCCATCCAGAAAGCGTACCGGTCAACAAGCTCGTGCCCATCGAGGGCACACCTCTGGCCAATAGC
GAAGAGCTCGATACGCTCGAGCTCGCCCGCGCCATTGCGGTCTGCCGCATCGTATTCCCGAAAAGCTGGGTGCGCCTCTC
TGCCGGGCGCGAAACGATGAGCGCCGAAGGCCAGGCCCTCTGCATCCTCGCCGGCGCCAACTCAATTTTTGTGGGCGACC
GCCTGCTGACAACTGCCAATCCCGCCATGGACAAGGACGCGCGCCTGCTCGAATCCGTGGGGGGTGGTTGCTCGAAGTGC
TAG

Upstream 100 bases:

>100_bases
GGCAGGGGTGGGCGTACCCATCTGATGAGCCCTGCTCTGGCCGCCCGTGCCGCCATCAATGGCGTGCTCGGATAATCTGT
TAACTGATCAGGATCATTTC

Downstream 100 bases:

>100_bases
GCTGAGTTTAGCAGAGCTTACCCGAGTTTGGTTGCCATTCTCCCGCTGCGGGCCGATATTCTCCCTTCAAGATAATCCTG
AAGGGAGAGTTCGGCATGGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTSSDLTPRQNWTREEIAAIYDLPLDQLFARALAVKAAHWADGAVQKSQLLSIKTGGCAENCGYCSQSASFKTGLKAEKL
LPVEEVVDAARRAKANGADRFCMGAAWRSLKDRDLPKVAEMISAVKAEGLETCVTLGMLEEGQAEALKEAGLDYYNHNLD
TSREYYSTVVTTRTFDDRIETLGRARQAGIKLCCGGILGMGESRSDRVGLIHELSLLSPHPESVPVNKLVPIEGTPLANS
EELDTLELARAIAVCRIVFPKSWVRLSAGRETMSAEGQALCILAGANSIFVGDRLLTTANPAMDKDARLLESVGGGCSKC

Sequences:

>Translated_320_residues
MTSSDLTPRQNWTREEIAAIYDLPLDQLFARALAVKAAHWADGAVQKSQLLSIKTGGCAENCGYCSQSASFKTGLKAEKL
LPVEEVVDAARRAKANGADRFCMGAAWRSLKDRDLPKVAEMISAVKAEGLETCVTLGMLEEGQAEALKEAGLDYYNHNLD
TSREYYSTVVTTRTFDDRIETLGRARQAGIKLCCGGILGMGESRSDRVGLIHELSLLSPHPESVPVNKLVPIEGTPLANS
EELDTLELARAIAVCRIVFPKSWVRLSAGRETMSAEGQALCILAGANSIFVGDRLLTTANPAMDKDARLLESVGGGCSKC
>Mature_319_residues
TSSDLTPRQNWTREEIAAIYDLPLDQLFARALAVKAAHWADGAVQKSQLLSIKTGGCAENCGYCSQSASFKTGLKAEKLL
PVEEVVDAARRAKANGADRFCMGAAWRSLKDRDLPKVAEMISAVKAEGLETCVTLGMLEEGQAEALKEAGLDYYNHNLDT
SREYYSTVVTTRTFDDRIETLGRARQAGIKLCCGGILGMGESRSDRVGLIHELSLLSPHPESVPVNKLVPIEGTPLANSE
ELDTLELARAIAVCRIVFPKSWVRLSAGRETMSAEGQALCILAGANSIFVGDRLLTTANPAMDKDARLLESVGGGCSKC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=52.9411764705882, Blast_Score=353, Evalue=9e-99,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=49.3670886075949, Blast_Score=325, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_HYPNA (Q0C661)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758782.1
- ProteinModelPortal:   Q0C661
- SMR:   Q0C661
- STRING:   Q0C661
- GeneID:   4289438
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0048
- NMPDR:   fig|228405.5.peg.48
- TIGR:   HNE_0048
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q0C661
- ProtClustDB:   CLSK2532159
- BioCyc:   HNEP81032:HNE_0048-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34482; Mature: 34351

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDLTPRQNWTREEIAAIYDLPLDQLFARALAVKAAHWADGAVQKSQLLSIKTGGCAE
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCC
NCGYCSQSASFKTGLKAEKLLPVEEVVDAARRAKANGADRFCMGAAWRSLKDRDLPKVAE
CCCCCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHH
MISAVKAEGLETCVTLGMLEEGQAEALKEAGLDYYNHNLDTSREYYSTVVTTRTFDDRIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCHHHHHH
TLGRARQAGIKLCCGGILGMGESRSDRVGLIHELSLLSPHPESVPVNKLVPIEGTPLANS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCC
EELDTLELARAIAVCRIVFPKSWVRLSAGRETMSAEGQALCILAGANSIFVGDRLLTTAN
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCEEEEEECCCEEEECCEEEECCC
PAMDKDARLLESVGGGCSKC
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSSDLTPRQNWTREEIAAIYDLPLDQLFARALAVKAAHWADGAVQKSQLLSIKTGGCAE
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCC
NCGYCSQSASFKTGLKAEKLLPVEEVVDAARRAKANGADRFCMGAAWRSLKDRDLPKVAE
CCCCCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHH
MISAVKAEGLETCVTLGMLEEGQAEALKEAGLDYYNHNLDTSREYYSTVVTTRTFDDRIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCHHHHHH
TLGRARQAGIKLCCGGILGMGESRSDRVGLIHELSLLSPHPESVPVNKLVPIEGTPLANS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCC
EELDTLELARAIAVCRIVFPKSWVRLSAGRETMSAEGQALCILAGANSIFVGDRLLTTAN
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCEEEEEECCCEEEECCEEEECCC
PAMDKDARLLESVGGGCSKC
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA