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The map label for this gene is oprM [H]

Identifier: 114799824

GI number: 114799824

Start: 198963

End: 200402

Strand: Reverse

Name: oprM [H]

Synonym: HNE_0213

Alternate gene names: 114799824

Gene position: 200402-198963 (Counterclockwise)

Preceding gene: 114799303

Following gene: 114797281

Centisome position: 5.41

GC content: 67.78

Gene sequence:

>1440_bases
ATGTCTGAGAAATGGGTCTCCCGGTACGCACGCCTGTCTGGCGGAGCGGTGGTCGTGTTGATGATCGCTGGCTGCGCCTC
GGTGCCGGACCGGCCGGTGGCTGAAGCCGCCCCTGCCGTTGGAGACGCCTTCCGGGCCGGCGCCAGTTCCGATCCTGTTT
CCTCCGAATGGTGGGCCGGCTTCAGCGACCCCGTCCTCGCCAGCCTGGTTAACACAACCCTTCAAGCCTCCCCCGCCCTG
CGGGGGGCAGACTTTTCGGTGGCTGAGGCCGAAGCGGCCCTGCGCCTGGCCCTGCTGGGCGCCAGCCCCACGGCCTCCAC
CCGCGCGGGCATCACGGCAGGCCGCCCCACCGGCACGTCCGCCGACCGTCTGAATGTCAGCGCAACCGGCACCCTGGCGG
CCAGCTGGGAATTCGACGCGTTCGGCCGCATTGCCGCCCTGGTGGAAGCGGCCCGCTATGACGCAGCCACCGCCCGCGAG
CTGCGGCGGGATATTGCCGTCACGCTCGCCTCCGAAACGGCCCTTGCCTACATCAACCTGCGCGGCGCCGAGGCGCGCCT
CGAAGTCGGCCGCCGCAACGCGCAGACCCAGGTGGAAGGACTCGAACTCGTCCGCACCCTTTTTGACAATGGCCGCGCCA
CACAGCTCGATATCGAGCAGGCCGAAACCCTTTACCGCACCACATTGGCCAGCCTCCCGGTGTACGAAGCCGACCGCGAA
GCAGCCATCAGCCGCCTTGCGGCGCTGACCGCAGCGCCGGCAAGCTCGGCAGACGCCCTCCTCCAGCAGCTCACGGCCTC
GCCCGGCCTGATCCCGGCCCTTGAGGCCCCGATGACAGCAGGCACACCGGAAGATCTCCTGCGCCGCCGGCCCGACATTC
GCGCAGCCGAAGCCCAGGTAGGCGCCGCCCTCGCCCTCGGGGAAGTGGCCCGCGCTGACCTCTTCCCGCGCATTACGCTG
AATGCAAATCTTCTCGGCCTCGTCCGCAATACCGGGGTAGCCGTCAGCGATGAGAGCCTCGGCTTCGACTTTGGCCCCGC
CATCACCTGGGCCGGTCCCGACCTGCGCGGCGTCTATGCTAATATTGACGCCACCGATGCGCGTACCGGCCAGATCATCG
CAAATTATCAGTCAACAGTGCTCGATGCTTTGGCAGATGCTGAAACAGCGCTGACAGATCTGGCATCGGAAAACCGCCGT
ACTGCTGATCTGGAAGCGGCCCTTGCTTCGGCCCGCCGGGCGCTCGACCTTGCCCGCCTGAGATACCGGGAAGGGCTCGA
TAGCTACCTTAATGTCCTCGACGCGCAGCGCTCGCTGCTCGACGCGGAAGACCGCCTCGCGGTCAACCGGGCAGAAACCG
CCCGGCGCGCCGTCCGCGCTTACAGAAGCCTGGGCGGTATCTGGACCGACGAAGAACTCGCCGCCTACCGGGCGGGATAG

Upstream 100 bases:

>100_bases
ATCTTTGATCTCAATCGTTTAAGGAAGCGCACGGCGGGTTAACTGCAAACTGCACTGCGTTGACTTGATCGGTAAGGTTC
TTCGTCTAGTTCGGAAAAAG

Downstream 100 bases:

>100_bases
GAAAATCCGATGCTGACGCTTAGCCGACCTAACATATTCAAAGCCGCCGGCGTGGCGCTTGTGGCCGCAATCCTGATTGC
GGCCCCTGCCCTGACCCAGC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSEKWVSRYARLSGGAVVVLMIAGCASVPDRPVAEAAPAVGDAFRAGASSDPVSSEWWAGFSDPVLASLVNTTLQASPAL
RGADFSVAEAEAALRLALLGASPTASTRAGITAGRPTGTSADRLNVSATGTLAASWEFDAFGRIAALVEAARYDAATARE
LRRDIAVTLASETALAYINLRGAEARLEVGRRNAQTQVEGLELVRTLFDNGRATQLDIEQAETLYRTTLASLPVYEADRE
AAISRLAALTAAPASSADALLQQLTASPGLIPALEAPMTAGTPEDLLRRRPDIRAAEAQVGAALALGEVARADLFPRITL
NANLLGLVRNTGVAVSDESLGFDFGPAITWAGPDLRGVYANIDATDARTGQIIANYQSTVLDALADAETALTDLASENRR
TADLEAALASARRALDLARLRYREGLDSYLNVLDAQRSLLDAEDRLAVNRAETARRAVRAYRSLGGIWTDEELAAYRAG

Sequences:

>Translated_479_residues
MSEKWVSRYARLSGGAVVVLMIAGCASVPDRPVAEAAPAVGDAFRAGASSDPVSSEWWAGFSDPVLASLVNTTLQASPAL
RGADFSVAEAEAALRLALLGASPTASTRAGITAGRPTGTSADRLNVSATGTLAASWEFDAFGRIAALVEAARYDAATARE
LRRDIAVTLASETALAYINLRGAEARLEVGRRNAQTQVEGLELVRTLFDNGRATQLDIEQAETLYRTTLASLPVYEADRE
AAISRLAALTAAPASSADALLQQLTASPGLIPALEAPMTAGTPEDLLRRRPDIRAAEAQVGAALALGEVARADLFPRITL
NANLLGLVRNTGVAVSDESLGFDFGPAITWAGPDLRGVYANIDATDARTGQIIANYQSTVLDALADAETALTDLASENRR
TADLEAALASARRALDLARLRYREGLDSYLNVLDAQRSLLDAEDRLAVNRAETARRAVRAYRSLGGIWTDEELAAYRAG
>Mature_478_residues
SEKWVSRYARLSGGAVVVLMIAGCASVPDRPVAEAAPAVGDAFRAGASSDPVSSEWWAGFSDPVLASLVNTTLQASPALR
GADFSVAEAEAALRLALLGASPTASTRAGITAGRPTGTSADRLNVSATGTLAASWEFDAFGRIAALVEAARYDAATAREL
RRDIAVTLASETALAYINLRGAEARLEVGRRNAQTQVEGLELVRTLFDNGRATQLDIEQAETLYRTTLASLPVYEADREA
AISRLAALTAAPASSADALLQQLTASPGLIPALEAPMTAGTPEDLLRRRPDIRAAEAQVGAALALGEVARADLFPRITLN
ANLLGLVRNTGVAVSDESLGFDFGPAITWAGPDLRGVYANIDATDARTGQIIANYQSTVLDALADAETALTDLASENRRT
ADLEAALASARRALDLARLRYREGLDSYLNVLDAQRSLLDAEDRLAVNRAETARRAVRAYRSLGGIWTDEELAAYRAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=27.8969957081545, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI2367348, Length=484, Percent_Identity=24.5867768595041, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50464; Mature: 50333

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKWVSRYARLSGGAVVVLMIAGCASVPDRPVAEAAPAVGDAFRAGASSDPVSSEWWAG
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCHHHCCC
FSDPVLASLVNTTLQASPALRGADFSVAEAEAALRLALLGASPTASTRAGITAGRPTGTS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
ADRLNVSATGTLAASWEFDAFGRIAALVEAARYDAATARELRRDIAVTLASETALAYINL
CCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEE
RGAEARLEVGRRNAQTQVEGLELVRTLFDNGRATQLDIEQAETLYRTTLASLPVYEADRE
CCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHH
AAISRLAALTAAPASSADALLQQLTASPGLIPALEAPMTAGTPEDLLRRRPDIRAAEAQV
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHH
GAALALGEVARADLFPRITLNANLLGLVRNTGVAVSDESLGFDFGPAITWAGPDLRGVYA
HHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEECCCCCCCCCCCEEEECCCCCCEEEE
NIDATDARTGQIIANYQSTVLDALADAETALTDLASENRRTADLEAALASARRALDLARL
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RYREGLDSYLNVLDAQRSLLDAEDRLAVNRAETARRAVRAYRSLGGIWTDEELAAYRAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SEKWVSRYARLSGGAVVVLMIAGCASVPDRPVAEAAPAVGDAFRAGASSDPVSSEWWAG
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCHHHCCC
FSDPVLASLVNTTLQASPALRGADFSVAEAEAALRLALLGASPTASTRAGITAGRPTGTS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
ADRLNVSATGTLAASWEFDAFGRIAALVEAARYDAATARELRRDIAVTLASETALAYINL
CCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEE
RGAEARLEVGRRNAQTQVEGLELVRTLFDNGRATQLDIEQAETLYRTTLASLPVYEADRE
CCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHH
AAISRLAALTAAPASSADALLQQLTASPGLIPALEAPMTAGTPEDLLRRRPDIRAAEAQV
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHH
GAALALGEVARADLFPRITLNANLLGLVRNTGVAVSDESLGFDFGPAITWAGPDLRGVYA
HHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEECCCCCCCCCCCEEEECCCCCCEEEE
NIDATDARTGQIIANYQSTVLDALADAETALTDLASENRRTADLEAALASARRALDLARL
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RYREGLDSYLNVLDAQRSLLDAEDRLAVNRAETARRAVRAYRSLGGIWTDEELAAYRAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]