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The map label for this gene is purB [H]

Identifier: 114799823

GI number: 114799823

Start: 2038174

End: 2039472

Strand: Direct

Name: purB [H]

Synonym: HNE_1969

Alternate gene names: 114799823

Gene position: 2038174-2039472 (Clockwise)

Preceding gene: 114799065

Following gene: 114799464

Centisome position: 55.01

GC content: 61.89

Gene sequence:

>1299_bases
ATGATCCCCCGTTATACCCGCCCGGAAATGGCCGCCATCTGGACGCCGGAGTCCCGCTTCCAGATCTGGCTCGACATCGA
AACGCTGGCTGCCGAAGCCATGGAACAGATGGGTCAGGTTCCCAAGGGCACGACCGCTGCCGTCCGGGAACGCGCGAAAT
TCGAGGTTGAGCGCATCGACGAGATCGAGCGCGAGGTGAAGCATGACGTGATCGCGTTCCTCACCAACGTTACCGAGCAT
GTTGGCGAAGAAGCCCGCTACCTGCATATGGGCATGACATCTTCGGATGTGCTCGACACTTGCCTGAATGTGCAGATGGT
GCGCGCCGCAGACTTGATGCTTGCCGGGATCGACCGCGTGCTTGCTGCGCTCGAGAAGCGCGCCTTCGAGCACAAGCTCA
CCCCCACCATTGGCCGCAGCCATGGCATCCATGCCGAGCCGACCACATTCGGCATCAAGCTCGCCACCTTTCATGCCGAG
TTCCAGCGCGCCCGTCAGCGCCTGTTGCTGGCCAAGGAAGAAGTCTCGACCTGCGCAATCTCCGGCGCGGTGGGCACGTT
CGCCAATATCGATCCGAAGGTTGAGGAACACGTCGCCGCCAAGCTCGGCCTGACGATCGAGCCTGTCTCGACGCAGGTTA
TCCCGCGAGACCGCCACGCCATGTATTTCGCCGTGCTCGGTGTGATCGCCTCATCTGTGGAGCGCCTCGCAACGGAAGTC
CGCCATCTTCAGCGCACCGAAGTTCTGGAAGCCGAGGAGTTCTTCTCCGCCGGCCAGAAGGGCTCCTCCGCGATGCCCCA
CAAGCGCAACCCGGTGTTGACCGAAAACCTGACCGGTCTTGCCCGCCTCGTGCGCTCGGCGGTCACCCCGGCGCTGGAAA
ACGTCGCCCTCTGGCATGAGCGGGACATCTCCCACTCCTCCGTGGAGCGCGGCATTGGCCCGGACGCGACGATCCACCTG
GATTTTGCCCTTCACCGCCTCGCGGGTGTTGTCGAAAACCTGGTCGTCTATCCGGAGAACATGCTGGCGACGATCAACTC
CATGGGCGGCCTGCACAATTCCCAGCGAATCCTGCTGGCGCTTGTTGAGAAGGGCGTCAGCCGCGAGGAGGCCTACCGCC
TCGTGCAACGCAATGCGATGCGCACCTGGAAGAAGGAAGGCCCGCTCAATGAGTTGCTAAAGGGCGACGCCGATGTCTCC
GCCCGCCTGACGGCCGCCGAAATCGATGATCTGTTCGATCTGGAATACCATTTCAAACATGTCGACACGATTTTCGCCCG
CGTTTTTGGCAAAGCCTGA

Upstream 100 bases:

>100_bases
GGGTGGCGAAATTGTGCCAACCGGCCTAGACGGCTGCGCAAAGCAATACGCCAACTATCACGCCCAAAATCACGCCTGAA
ACCACGAACGAGAGCGAACC

Downstream 100 bases:

>100_bases
CGCACCTGCAACAGAACGCATTAATCAGGGCAGGCTTTCGGGCCTGACCTTTTCTTTGCATGCCCGGCGGTTGACCGCAC
GCGCTTACGGGTGAACATCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIPRYTRPEMAAIWTPESRFQIWLDIETLAAEAMEQMGQVPKGTTAAVRERAKFEVERIDEIEREVKHDVIAFLTNVTEH
VGEEARYLHMGMTSSDVLDTCLNVQMVRAADLMLAGIDRVLAALEKRAFEHKLTPTIGRSHGIHAEPTTFGIKLATFHAE
FQRARQRLLLAKEEVSTCAISGAVGTFANIDPKVEEHVAAKLGLTIEPVSTQVIPRDRHAMYFAVLGVIASSVERLATEV
RHLQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRSAVTPALENVALWHERDISHSSVERGIGPDATIHL
DFALHRLAGVVENLVVYPENMLATINSMGGLHNSQRILLALVEKGVSREEAYRLVQRNAMRTWKKEGPLNELLKGDADVS
ARLTAAEIDDLFDLEYHFKHVDTIFARVFGKA

Sequences:

>Translated_432_residues
MIPRYTRPEMAAIWTPESRFQIWLDIETLAAEAMEQMGQVPKGTTAAVRERAKFEVERIDEIEREVKHDVIAFLTNVTEH
VGEEARYLHMGMTSSDVLDTCLNVQMVRAADLMLAGIDRVLAALEKRAFEHKLTPTIGRSHGIHAEPTTFGIKLATFHAE
FQRARQRLLLAKEEVSTCAISGAVGTFANIDPKVEEHVAAKLGLTIEPVSTQVIPRDRHAMYFAVLGVIASSVERLATEV
RHLQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRSAVTPALENVALWHERDISHSSVERGIGPDATIHL
DFALHRLAGVVENLVVYPENMLATINSMGGLHNSQRILLALVEKGVSREEAYRLVQRNAMRTWKKEGPLNELLKGDADVS
ARLTAAEIDDLFDLEYHFKHVDTIFARVFGKA
>Mature_432_residues
MIPRYTRPEMAAIWTPESRFQIWLDIETLAAEAMEQMGQVPKGTTAAVRERAKFEVERIDEIEREVKHDVIAFLTNVTEH
VGEEARYLHMGMTSSDVLDTCLNVQMVRAADLMLAGIDRVLAALEKRAFEHKLTPTIGRSHGIHAEPTTFGIKLATFHAE
FQRARQRLLLAKEEVSTCAISGAVGTFANIDPKVEEHVAAKLGLTIEPVSTQVIPRDRHAMYFAVLGVIASSVERLATEV
RHLQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRSAVTPALENVALWHERDISHSSVERGIGPDATIHL
DFALHRLAGVVENLVVYPENMLATINSMGGLHNSQRILLALVEKGVSREEAYRLVQRNAMRTWKKEGPLNELLKGDADVS
ARLTAAEIDDLFDLEYHFKHVDTIFARVFGKA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=431, Percent_Identity=29.0023201856148, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=29.7229219143577, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787376, Length=280, Percent_Identity=28.2142857142857, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=26.5822784810127, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=330, Percent_Identity=27.8787878787879, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=412, Percent_Identity=29.6116504854369, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24647570, Length=413, Percent_Identity=26.3922518159806, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48173; Mature: 48173

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYTRPEMAAIWTPESRFQIWLDIETLAAEAMEQMGQVPKGTTAAVRERAKFEVERID
CCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EIEREVKHDVIAFLTNVTEHVGEEARYLHMGMTSSDVLDTCLNVQMVRAADLMLAGIDRV
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALEKRAFEHKLTPTIGRSHGIHAEPTTFGIKLATFHAEFQRARQRLLLAKEEVSTCAI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPKVEEHVAAKLGLTIEPVSTQVIPRDRHAMYFAVLGVIASSVERLATEV
HCCHHHCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRSAVTPALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERGIGPDATIHLDFALHRLAGVVENLVVYPENMLATINSMGGLHNSQRILLA
CCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
LVEKGVSREEAYRLVQRNAMRTWKKEGPLNELLKGDADVSARLTAAEIDDLFDLEYHFKH
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHH
VDTIFARVFGKA
HHHHHHHHHCCC
>Mature Secondary Structure
MIPRYTRPEMAAIWTPESRFQIWLDIETLAAEAMEQMGQVPKGTTAAVRERAKFEVERID
CCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EIEREVKHDVIAFLTNVTEHVGEEARYLHMGMTSSDVLDTCLNVQMVRAADLMLAGIDRV
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALEKRAFEHKLTPTIGRSHGIHAEPTTFGIKLATFHAEFQRARQRLLLAKEEVSTCAI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPKVEEHVAAKLGLTIEPVSTQVIPRDRHAMYFAVLGVIASSVERLATEV
HCCHHHCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRSAVTPALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERGIGPDATIHLDFALHRLAGVVENLVVYPENMLATINSMGGLHNSQRILLA
CCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
LVEKGVSREEAYRLVQRNAMRTWKKEGPLNELLKGDADVSARLTAAEIDDLFDLEYHFKH
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHH
VDTIFARVFGKA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]