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The map label for this gene is trpB [H]

Identifier: 114799821

GI number: 114799821

Start: 3611155

End: 3612366

Strand: Reverse

Name: trpB [H]

Synonym: HNE_3476

Alternate gene names: 114799821

Gene position: 3612366-3611155 (Counterclockwise)

Preceding gene: 114797372

Following gene: 114798841

Centisome position: 97.5

GC content: 62.05

Gene sequence:

>1212_bases
ATGGACCTGCGCAATACCTGGGATCAATGGCCGGACGCAAATGGCCGGTTTGGTGAATTTGGCGGACGGTATGTGGCCGA
GACGCTGATGCCGCTGATCCTGGATCTGGAGCGTGAATATCGCGCGGCCAAGCAGGACCCGGCTTTCAAGGCGGAGATGG
ACGATTTGTGGGCCCATTATGTGGGCCGCCCGTCGCCGCTGTATTTCGCTGAGCGCCTGACCGAGCATTTCGGCGGAGCG
AAAATCTATTTCAAACGCGATGAGCTCAATCACACCGGCGCGCACAAGATCAACAACTGCCTGGGTCAGGTGCTGCTGGC
GCGGCGGATGGGCAAGAAGCGTATCATTGCCGAAACCGGCGCGGGCCAGCACGGCGTGGCAACAGCCACGATCTGTGCCC
GCTTTGGCCTCGATTGCGTGGTGTTCATGGGCGAGACCGATGTGGAGCGCCAGAAACCCAACGTGTTCCGGATGCGCCTT
CTGGGCGCGCAGATTGTTCCGGTTTCCTCAGGCACGGGCACGCTGAAAGATGCGATGAACGAAGCCCTGCGCGACTGGGT
GACGAATGTGGATGACACCTTCTATTGCATCGGCACGGCGGCCGGCCCGCACCCCTATCCGGAGATGGTGCGTGATTTCC
AGTCCATCATCGGCAAGGAAGCGCGCGCGCAGATTCTCGAGCGGGAAGGGCGCCTGCCGGACGCTGTGATGGCGTGCATC
GGGGGCGGCTCCAACGCGATTGGCCTCTTCCATCCTTTCATCGAGGATGAGGGCGTGCGCCTGATTGGCGTGGAAGCGGC
CGGGCATGGCATCGAGACCGGAGAGCATGCCGCTGCGCTGAACGGTGGCAAGCCCGGCATCCTGCATGGCAACCGGACAT
ATCTGCTGCAGACCGATGATGGTCAGATCATTGACGCGCATTCGATTTCGGCAGGCCTGGATTATCCCGGCATTGGCCCG
GAGCACGCCTTCCTGCGCGATGCGGGAAGGGCTGAATATCTGTCGTGTACGGACAAGGAGGCGCTTGATGCGTTCCAGCT
CTGCACGCGGCTTGAAGGCATCATTCCGGCGCTGGAGCCTTCGCACGCGATCGCGCGGGTCGGCGAGGTGGCAAAGGATC
TGGGCAAGGACGGCCTGCTGATCCTCAACATGTGCGGGCGCGGGGACAAGGATGTGTTCTCCGTCGCCAAGGCGCTGGGA
GTGGATGTATGA

Upstream 100 bases:

>100_bases
TAAAGGACAGGGAACTCGTCCGCGCGTTCATCCGCGCTGCGAAGGCAAGTGAGCAGCCCTGAGCGCGATTCATCGCGCAG
CGGGAATCAGAGGAAAAGTG

Downstream 100 bases:

>100_bases
GCAATCTCATCCTTGTGCGCATTACATGCCCTTCGCGCCGCGTGGCTGAAGATATTGCAGAAGTGGCCCTTGAGCATCGC
CTGGCGGCCTGCGCCAATCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MDLRNTWDQWPDANGRFGEFGGRYVAETLMPLILDLEREYRAAKQDPAFKAEMDDLWAHYVGRPSPLYFAERLTEHFGGA
KIYFKRDELNHTGAHKINNCLGQVLLARRMGKKRIIAETGAGQHGVATATICARFGLDCVVFMGETDVERQKPNVFRMRL
LGAQIVPVSSGTGTLKDAMNEALRDWVTNVDDTFYCIGTAAGPHPYPEMVRDFQSIIGKEARAQILEREGRLPDAVMACI
GGGSNAIGLFHPFIEDEGVRLIGVEAAGHGIETGEHAAALNGGKPGILHGNRTYLLQTDDGQIIDAHSISAGLDYPGIGP
EHAFLRDAGRAEYLSCTDKEALDAFQLCTRLEGIIPALEPSHAIARVGEVAKDLGKDGLLILNMCGRGDKDVFSVAKALG
VDV

Sequences:

>Translated_403_residues
MDLRNTWDQWPDANGRFGEFGGRYVAETLMPLILDLEREYRAAKQDPAFKAEMDDLWAHYVGRPSPLYFAERLTEHFGGA
KIYFKRDELNHTGAHKINNCLGQVLLARRMGKKRIIAETGAGQHGVATATICARFGLDCVVFMGETDVERQKPNVFRMRL
LGAQIVPVSSGTGTLKDAMNEALRDWVTNVDDTFYCIGTAAGPHPYPEMVRDFQSIIGKEARAQILEREGRLPDAVMACI
GGGSNAIGLFHPFIEDEGVRLIGVEAAGHGIETGEHAAALNGGKPGILHGNRTYLLQTDDGQIIDAHSISAGLDYPGIGP
EHAFLRDAGRAEYLSCTDKEALDAFQLCTRLEGIIPALEPSHAIARVGEVAKDLGKDGLLILNMCGRGDKDVFSVAKALG
VDV
>Mature_403_residues
MDLRNTWDQWPDANGRFGEFGGRYVAETLMPLILDLEREYRAAKQDPAFKAEMDDLWAHYVGRPSPLYFAERLTEHFGGA
KIYFKRDELNHTGAHKINNCLGQVLLARRMGKKRIIAETGAGQHGVATATICARFGLDCVVFMGETDVERQKPNVFRMRL
LGAQIVPVSSGTGTLKDAMNEALRDWVTNVDDTFYCIGTAAGPHPYPEMVRDFQSIIGKEARAQILEREGRLPDAVMACI
GGGSNAIGLFHPFIEDEGVRLIGVEAAGHGIETGEHAAALNGGKPGILHGNRTYLLQTDDGQIIDAHSISAGLDYPGIGP
EHAFLRDAGRAEYLSCTDKEALDAFQLCTRLEGIIPALEPSHAIARVGEVAKDLGKDGLLILNMCGRGDKDVFSVAKALG
VDV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=383, Percent_Identity=57.1801566579634, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=57.6623376623377, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43887; Mature: 43887

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRNTWDQWPDANGRFGEFGGRYVAETLMPLILDLEREYRAAKQDPAFKAEMDDLWAHY
CCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VGRPSPLYFAERLTEHFGGAKIYFKRDELNHTGAHKINNCLGQVLLARRMGKKRIIAETG
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
AGQHGVATATICARFGLDCVVFMGETDVERQKPNVFRMRLLGAQIVPVSSGTGTLKDAMN
CCCCCHHHHHHHHHHCCCEEEEECCCCCHHCCCCHHHHHHHCCEEEEECCCCCHHHHHHH
EALRDWVTNVDDTFYCIGTAAGPHPYPEMVRDFQSIIGKEARAQILEREGRLPDAVMACI
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
GGGSNAIGLFHPFIEDEGVRLIGVEAAGHGIETGEHAAALNGGKPGILHGNRTYLLQTDD
CCCCCCEEHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCEEECCEEEEEECCC
GQIIDAHSISAGLDYPGIGPEHAFLRDAGRAEYLSCTDKEALDAFQLCTRLEGIIPALEP
CCEEECCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
SHAIARVGEVAKDLGKDGLLILNMCGRGDKDVFSVAKALGVDV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRNTWDQWPDANGRFGEFGGRYVAETLMPLILDLEREYRAAKQDPAFKAEMDDLWAHY
CCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VGRPSPLYFAERLTEHFGGAKIYFKRDELNHTGAHKINNCLGQVLLARRMGKKRIIAETG
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
AGQHGVATATICARFGLDCVVFMGETDVERQKPNVFRMRLLGAQIVPVSSGTGTLKDAMN
CCCCCHHHHHHHHHHCCCEEEEECCCCCHHCCCCHHHHHHHCCEEEEECCCCCHHHHHHH
EALRDWVTNVDDTFYCIGTAAGPHPYPEMVRDFQSIIGKEARAQILEREGRLPDAVMACI
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
GGGSNAIGLFHPFIEDEGVRLIGVEAAGHGIETGEHAAALNGGKPGILHGNRTYLLQTDD
CCCCCCEEHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCEEECCEEEEEECCC
GQIIDAHSISAGLDYPGIGPEHAFLRDAGRAEYLSCTDKEALDAFQLCTRLEGIIPALEP
CCEEECCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
SHAIARVGEVAKDLGKDGLLILNMCGRGDKDVFSVAKALGVDV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2828322; 11259647 [H]