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The map label for this gene is trpF

Identifier: 114797372

GI number: 114797372

Start: 3612405

End: 3613040

Strand: Reverse

Name: trpF

Synonym: HNE_3477

Alternate gene names: 114797372

Gene position: 3613040-3612405 (Counterclockwise)

Preceding gene: 114800216

Following gene: 114799821

Centisome position: 97.52

GC content: 63.68

Gene sequence:

>636_bases
ATGGCGAAGGTTAAGATCTGCGGGCTCAAAGATCCGGATATGGTGGCGTTTGCTGCCCGTGAGGGGGCGGACTGGGTCGG
CTTTGTGTTTGCGCCGAGTGTGCGGCAGGTGACGCTGGCGGCGGCAGAAACCCTTTTACTTTCAGTGGGGAAGGCCACCC
CTGTCGCGTTGATGGTTGACCCGTCTGACGCTGAGGCGCAGGCGGTTGCCGCCCTGGGGTTCCCCATTCTTCAGTTGCAC
GGGCAGGAAACGCCGGCCCGCGCTGCAGAGCTCAAAAGCCTGACAGGATGCGCCATCTGGAAGGCCATCGGCGTACAAAC
CCGTGACAATCTGGGACAAATCGGGACATTTCCGGACATCGACGGACTTCTTCTGGACGCCAAGCCGCCAGAAGGCGCCA
CCATTGCCGGCGGGCATGGCGCGGCGTTTGACTGGTCGATCCTCAAGGGTTGGACGGCGCCCAAGCCCTGGCTGCTGGCA
GGCGGTCTGACACCGGAGAATGTGGCGGAAGCCATTGCTGCGACGGGTGCGCCCGGCGTGGATGTGTCCTCTGGCGTCGA
GCGTATCCGGGGGCTAAAGGACAGGGAACTCGTCCGCGCGTTCATCCGCGCTGCGAAGGCAAGTGAGCAGCCCTGA

Upstream 100 bases:

>100_bases
TAGACGGCAACGTCTCCAGGGTTCGAATCCCTGTCTCTCCGCCAGTCATTCTGCTGAAATGCACGTGGACGGCCGCCGGG
CTCTGGCGTAACGGGCTGTT

Downstream 100 bases:

>100_bases
GCGCGATTCATCGCGCAGCGGGAATCAGAGGAAAAGTGATGGACCTGCGCAATACCTGGGATCAATGGCCGGACGCAAAT
GGCCGGTTTGGTGAATTTGG

Product: N-(5'phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAKVKICGLKDPDMVAFAAREGADWVGFVFAPSVRQVTLAAAETLLLSVGKATPVALMVDPSDAEAQAVAALGFPILQLH
GQETPARAAELKSLTGCAIWKAIGVQTRDNLGQIGTFPDIDGLLLDAKPPEGATIAGGHGAAFDWSILKGWTAPKPWLLA
GGLTPENVAEAIAATGAPGVDVSSGVERIRGLKDRELVRAFIRAAKASEQP

Sequences:

>Translated_211_residues
MAKVKICGLKDPDMVAFAAREGADWVGFVFAPSVRQVTLAAAETLLLSVGKATPVALMVDPSDAEAQAVAALGFPILQLH
GQETPARAAELKSLTGCAIWKAIGVQTRDNLGQIGTFPDIDGLLLDAKPPEGATIAGGHGAAFDWSILKGWTAPKPWLLA
GGLTPENVAEAIAATGAPGVDVSSGVERIRGLKDRELVRAFIRAAKASEQP
>Mature_210_residues
AKVKICGLKDPDMVAFAAREGADWVGFVFAPSVRQVTLAAAETLLLSVGKATPVALMVDPSDAEAQAVAALGFPILQLHG
QETPARAAELKSLTGCAIWKAIGVQTRDNLGQIGTFPDIDGLLLDAKPPEGATIAGGHGAAFDWSILKGWTAPKPWLLAG
GLTPENVAEAIAATGAPGVDVSSGVERIRGLKDRELVRAFIRAAKASEQP

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=220, Percent_Identity=31.3636363636364, Blast_Score=68, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320210, Length=226, Percent_Identity=30.5309734513274, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_HYPNA (Q0BWJ4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762149.1
- ProteinModelPortal:   Q0BWJ4
- SMR:   Q0BWJ4
- STRING:   Q0BWJ4
- GeneID:   4287303
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3477
- NMPDR:   fig|228405.5.peg.3330
- TIGR:   HNE_3477
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   ETTGITS
- PhylomeDB:   Q0BWJ4
- BioCyc:   HNEP81032:HNE_3477-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 21856; Mature: 21725

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVKICGLKDPDMVAFAAREGADWVGFVFAPSVRQVTLAAAETLLLSVGKATPVALMVD
CCCEEECCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
PSDAEAQAVAALGFPILQLHGQETPARAAELKSLTGCAIWKAIGVQTRDNLGQIGTFPDI
CCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGLLLDAKPPEGATIAGGHGAAFDWSILKGWTAPKPWLLAGGLTPENVAEAIAATGAPGV
CCEEEECCCCCCCEEECCCCCEEEHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
DVSSGVERIRGLKDRELVRAFIRAAKASEQP
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKVKICGLKDPDMVAFAAREGADWVGFVFAPSVRQVTLAAAETLLLSVGKATPVALMVD
CCEEECCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
PSDAEAQAVAALGFPILQLHGQETPARAAELKSLTGCAIWKAIGVQTRDNLGQIGTFPDI
CCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGLLLDAKPPEGATIAGGHGAAFDWSILKGWTAPKPWLLAGGLTPENVAEAIAATGAPGV
CCEEEECCCCCCCEEECCCCCEEEHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
DVSSGVERIRGLKDRELVRAFIRAAKASEQP
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA