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The map label for this gene is hdhA [C]

Identifier: 114799757

GI number: 114799757

Start: 2289126

End: 2290040

Strand: Reverse

Name: hdhA [C]

Synonym: HNE_2192

Alternate gene names: 114799757

Gene position: 2290040-2289126 (Counterclockwise)

Preceding gene: 114797925

Following gene: 114797259

Centisome position: 61.81

GC content: 63.61

Gene sequence:

>915_bases
ATGGAAAAATGGACGCTTGAGAATATCCCATCGCAATCCGGTCGCTCCGCGGTTGTTACCGGCACGGGCGGACTTGGTTT
CGAGGATGCGTTGGCTCTTGCGCGCGCCGGCGCTGAGGTCGTGATCGCAGGACGCAATTCTGAAAAGGGCGCTGCGGCGG
TTACCGCGATCCGGAAGCTCGTTCCGAGCGCCCGGGTACGGTTTGGCCTGGTCGATCTGGCAAACCTGTCATCCGTCTCC
GCCTTTGCTGCAGAAATCGCATGGGAGCGGACCAGCCTTGACCTGCTGATCAACAATGCGGCCGTGATGAATCCGCCCGC
GCGGCGCACGACCCGTGACGGGTTCGAGCTGCAGCTTGGAACCAACTTTCTTGGTCATTTTGCGCTGACAGCGCACCTGT
TGCCGCTCCTGAAGCGCGGCGCGGCGCCGCGGATCGTAACCGTCAGCAGCGTCGCCGCGCGACAGGGCGCGATCAATTTC
GATGACCTGCAGGCGACGCGCACCTACCATCCGATGACGGTCTACAGCCAGTCAAAGCTCGCCTGTCTGATGTTTGCCCT
CGAACTGAGCCGGCGCAGCCGGGCGGGAGGTTGGGGTGTCGCCAGTCTTGCCGCGCATCCGGGCGTTACCCGGACAGATC
TCATCGTGAATGGCGCGGGGCCATCGAGCCTCAATGGCAGAATTCGGCGCTTCCTGCCATTCCTGTTTCAGCCCGCATGG
CAGGGGGCGTTGCCGACGCTGTACGCGGCGACCGATCCCGAAGCGCGCGACGGTGCCTATTACGGACCCGGCGGACCCGG
TGGAATACGGGGCTATCCGGCGGAGGAAAGGCCGCCCAAACACGCACTGGACGAAGCCGTCGCGGCCCGCCTCTGGAATG
TATCGGTCGGGTTGACGGACGCCAAATTCCTATAA

Upstream 100 bases:

>100_bases
GGCACTTTGTCCGCGGCCGGTCATTTTTGAAGCCGAATTCCGGCGCGGGTGATTTGAGAAAATGAGCGCTCGAATCAAGG
TTGAAAGGGTGAGATCTCCC

Downstream 100 bases:

>100_bases
TGTAATATAGTCTGTACAGGAATTGTATGTCCGGCGGGGCAGGCGGGGGTGCCCGGTGTTCAGACACGTGTCCAGACCAG
GCTTGCCTTGCGCCAGCCCG

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MEKWTLENIPSQSGRSAVVTGTGGLGFEDALALARAGAEVVIAGRNSEKGAAAVTAIRKLVPSARVRFGLVDLANLSSVS
AFAAEIAWERTSLDLLINNAAVMNPPARRTTRDGFELQLGTNFLGHFALTAHLLPLLKRGAAPRIVTVSSVAARQGAINF
DDLQATRTYHPMTVYSQSKLACLMFALELSRRSRAGGWGVASLAAHPGVTRTDLIVNGAGPSSLNGRIRRFLPFLFQPAW
QGALPTLYAATDPEARDGAYYGPGGPGGIRGYPAEERPPKHALDEAVAARLWNVSVGLTDAKFL

Sequences:

>Translated_304_residues
MEKWTLENIPSQSGRSAVVTGTGGLGFEDALALARAGAEVVIAGRNSEKGAAAVTAIRKLVPSARVRFGLVDLANLSSVS
AFAAEIAWERTSLDLLINNAAVMNPPARRTTRDGFELQLGTNFLGHFALTAHLLPLLKRGAAPRIVTVSSVAARQGAINF
DDLQATRTYHPMTVYSQSKLACLMFALELSRRSRAGGWGVASLAAHPGVTRTDLIVNGAGPSSLNGRIRRFLPFLFQPAW
QGALPTLYAATDPEARDGAYYGPGGPGGIRGYPAEERPPKHALDEAVAARLWNVSVGLTDAKFL
>Mature_304_residues
MEKWTLENIPSQSGRSAVVTGTGGLGFEDALALARAGAEVVIAGRNSEKGAAAVTAIRKLVPSARVRFGLVDLANLSSVS
AFAAEIAWERTSLDLLINNAAVMNPPARRTTRDGFELQLGTNFLGHFALTAHLLPLLKRGAAPRIVTVSSVAARQGAINF
DDLQATRTYHPMTVYSQSKLACLMFALELSRRSRAGGWGVASLAAHPGVTRTDLIVNGAGPSSLNGRIRRFLPFLFQPAW
QGALPTLYAATDPEARDGAYYGPGGPGGIRGYPAEERPPKHALDEAVAARLWNVSVGLTDAKFL

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI193804850, Length=297, Percent_Identity=36.7003367003367, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI225579078, Length=293, Percent_Identity=41.296928327645, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI10190746, Length=298, Percent_Identity=36.9127516778523, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI186928839, Length=288, Percent_Identity=39.5833333333333, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI166795268, Length=288, Percent_Identity=36.1111111111111, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI19923931, Length=258, Percent_Identity=41.4728682170543, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI7706523, Length=302, Percent_Identity=33.7748344370861, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI146231950, Length=290, Percent_Identity=36.551724137931, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI72534732, Length=166, Percent_Identity=31.9277108433735, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI13375997, Length=166, Percent_Identity=31.9277108433735, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558006, Length=301, Percent_Identity=31.5614617940199, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17532805, Length=265, Percent_Identity=34.7169811320755, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71992402, Length=299, Percent_Identity=31.1036789297659, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193207620, Length=302, Percent_Identity=32.1192052980132, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17532791, Length=318, Percent_Identity=29.2452830188679, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI72001364, Length=303, Percent_Identity=30.03300330033, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17535059, Length=302, Percent_Identity=28.1456953642384, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17505416, Length=272, Percent_Identity=29.7794117647059, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32565532, Length=216, Percent_Identity=31.4814814814815, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115533172, Length=288, Percent_Identity=28.8194444444444, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115533174, Length=288, Percent_Identity=28.8194444444444, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324820, Length=321, Percent_Identity=29.2834890965732, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573296, Length=256, Percent_Identity=40.625, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI22024069, Length=290, Percent_Identity=38.2758620689655, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19921754, Length=286, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24586328, Length=291, Percent_Identity=38.1443298969072, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161076371, Length=256, Percent_Identity=38.671875, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45554248, Length=253, Percent_Identity=39.1304347826087, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640117, Length=253, Percent_Identity=39.1304347826087, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656154, Length=278, Percent_Identity=35.6115107913669, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922628, Length=278, Percent_Identity=35.6115107913669, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553409, Length=289, Percent_Identity=31.1418685121107, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647948, Length=289, Percent_Identity=31.1418685121107, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647946, Length=291, Percent_Identity=30.9278350515464, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129347, Length=297, Percent_Identity=32.6599326599327, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24647931, Length=270, Percent_Identity=31.4814814814815, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 32256; Mature: 32256

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKWTLENIPSQSGRSAVVTGTGGLGFEDALALARAGAEVVIAGRNSEKGAAAVTAIRKL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VPSARVRFGLVDLANLSSVSAFAAEIAWERTSLDLLINNAAVMNPPARRTTRDGFELQLG
CCHHHHEECEEECCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCEEEEEC
TNFLGHFALTAHLLPLLKRGAAPRIVTVSSVAARQGAINFDDLQATRTYHPMTVYSQSKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCHHHHHHHCCCCCEEEECCCHH
ACLMFALELSRRSRAGGWGVASLAAHPGVTRTDLIVNGAGPSSLNGRIRRFLPFLFQPAW
HHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
QGALPTLYAATDPEARDGAYYGPGGPGGIRGYPAEERPPKHALDEAVAARLWNVSVGLTD
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCC
AKFL
CCCC
>Mature Secondary Structure
MEKWTLENIPSQSGRSAVVTGTGGLGFEDALALARAGAEVVIAGRNSEKGAAAVTAIRKL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VPSARVRFGLVDLANLSSVSAFAAEIAWERTSLDLLINNAAVMNPPARRTTRDGFELQLG
CCHHHHEECEEECCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCEEEEEC
TNFLGHFALTAHLLPLLKRGAAPRIVTVSSVAARQGAINFDDLQATRTYHPMTVYSQSKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCHHHHHHHCCCCCEEEECCCHH
ACLMFALELSRRSRAGGWGVASLAAHPGVTRTDLIVNGAGPSSLNGRIRRFLPFLFQPAW
HHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
QGALPTLYAATDPEARDGAYYGPGGPGGIRGYPAEERPPKHALDEAVAARLWNVSVGLTD
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCC
AKFL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]