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The map label for this gene is nolF [H]

Identifier: 114797259

GI number: 114797259

Start: 2287828

End: 2288928

Strand: Reverse

Name: nolF [H]

Synonym: HNE_2191

Alternate gene names: 114797259

Gene position: 2288928-2287828 (Counterclockwise)

Preceding gene: 114799757

Following gene: 114797722

Centisome position: 61.78

GC content: 66.3

Gene sequence:

>1101_bases
ATGCATTCGATCACCCTGAAGACTCTTTTATTCACCCCGGTTTGCAGCGGTTTGCTGCTCTTTCTACTGAGCGCGCTGCC
GGCTTATGCGCAGTCCGGCGCTGCGCGGGTCAGCGTCGCTGCGGTCGAAACCGAGGAGATTGTCGAGCAACTGACGCTCA
CCGGCACCGTTACTGCGGCGCGACGATCGCCGATCTCGTCCGAAATCGCCGGCAAGATCGCGCAGCTTCAAGTCGAGGAA
GGCGATCGTATCCCATCAGGGGGTGTGCTGGCGACGCTCGATGCGCGGCGCGCGCGCCTTGCCTCCCGGCAAGCTCAGGC
CGCCATGCGACAGGCAGAAGCCGAACTTGTAGACGCCGAACGGCGGCTGGAGATGGCGCGCCGGCTCGCCGAGCGGCGCG
CGGTTTCCGAGGACGAACGCGGCACGCTGGAGACCGAGGTCCAGACGGATCGGGCTGTGCTGGAACGTCTGCGAGCCGAA
GCCGGCGAACAGGCGCTGCTGATTGACATGCATGAGATCCGGGCGCCATTCGCCGGGGTCGTTGCTCAGCGTTTTACCGA
AGCCGGGGCATGGGTGGAGCCGGGTGATCCGGTGGTCGAGCTGGTGGATCTCGACCAGCTGCGGATCGATGTCGCCGCGC
CGCAGGAATTCTATCCGCGCCTCGCCGGCGGCGCGCGCGCAACCGTCCGTCTGGACGCGCAACCGGGCAGCGCCCTTGAG
GGCACACTCCTCAGCGCCGTGCCTGTCGCCAGTCCGGGGTCTCGGACTTTTCTTGCCCGGATCGTCGTCTCGAATGAAAA
TGCTGCCCTGATGCCGGGCCTGTCGGCGAAAGTGACCCTGCATCTGCCCACCGGCAATCGCCATCCTGTCATTCCGCGCG
ATGCGCTGATCCGTCATCCCGACGGGCGCACGACCGCCTGGGTCGTGGTCAGTGCCGGGGGCGCCCTGACCGCGCAGGAG
CGCAATATCGTAACCGGTCGCGCTTTTGCGGGCCGGGTGGAAGTCCGCCGCGGGCTGAGCGACGGCGATTCTGTCGTCAC
AGAGGGCAATGAGGGCCTTCAGCCGGGACAGGCCGTGGAAATTGTCCGCAACGCATCCTGA

Upstream 100 bases:

>100_bases
CCGGTTCGATCCCGCCCAATCCTTTCAGAATCGTCATTTTGACGTCAGGCGACCGTCAACCCTGTTGGTTAGGGTAATCG
GGCCAAGCGCAGGATCCCCG

Downstream 100 bases:

>100_bases
CGCATACAGCCGTCCGTTCTCTGTCATCCTAGCTGAGCAAGGATTCCCCCTACATGTTTGAGGCAATTGTCAAACGCGGA
ACGCTGATGACGGTGATCCT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MHSITLKTLLFTPVCSGLLLFLLSALPAYAQSGAARVSVAAVETEEIVEQLTLTGTVTAARRSPISSEIAGKIAQLQVEE
GDRIPSGGVLATLDARRARLASRQAQAAMRQAEAELVDAERRLEMARRLAERRAVSEDERGTLETEVQTDRAVLERLRAE
AGEQALLIDMHEIRAPFAGVVAQRFTEAGAWVEPGDPVVELVDLDQLRIDVAAPQEFYPRLAGGARATVRLDAQPGSALE
GTLLSAVPVASPGSRTFLARIVVSNENAALMPGLSAKVTLHLPTGNRHPVIPRDALIRHPDGRTTAWVVVSAGGALTAQE
RNIVTGRAFAGRVEVRRGLSDGDSVVTEGNEGLQPGQAVEIVRNAS

Sequences:

>Translated_366_residues
MHSITLKTLLFTPVCSGLLLFLLSALPAYAQSGAARVSVAAVETEEIVEQLTLTGTVTAARRSPISSEIAGKIAQLQVEE
GDRIPSGGVLATLDARRARLASRQAQAAMRQAEAELVDAERRLEMARRLAERRAVSEDERGTLETEVQTDRAVLERLRAE
AGEQALLIDMHEIRAPFAGVVAQRFTEAGAWVEPGDPVVELVDLDQLRIDVAAPQEFYPRLAGGARATVRLDAQPGSALE
GTLLSAVPVASPGSRTFLARIVVSNENAALMPGLSAKVTLHLPTGNRHPVIPRDALIRHPDGRTTAWVVVSAGGALTAQE
RNIVTGRAFAGRVEVRRGLSDGDSVVTEGNEGLQPGQAVEIVRNAS
>Mature_366_residues
MHSITLKTLLFTPVCSGLLLFLLSALPAYAQSGAARVSVAAVETEEIVEQLTLTGTVTAARRSPISSEIAGKIAQLQVEE
GDRIPSGGVLATLDARRARLASRQAQAAMRQAEAELVDAERRLEMARRLAERRAVSEDERGTLETEVQTDRAVLERLRAE
AGEQALLIDMHEIRAPFAGVVAQRFTEAGAWVEPGDPVVELVDLDQLRIDVAAPQEFYPRLAGGARATVRLDAQPGSALE
GTLLSAVPVASPGSRTFLARIVVSNENAALMPGLSAKVTLHLPTGNRHPVIPRDALIRHPDGRTTAWVVVSAGGALTAQE
RNIVTGRAFAGRVEVRRGLSDGDSVVTEGNEGLQPGQAVEIVRNAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=356, Percent_Identity=27.5280898876405, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081796, Length=312, Percent_Identity=26.2820512820513, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38965; Mature: 38965

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSITLKTLLFTPVCSGLLLFLLSALPAYAQSGAARVSVAAVETEEIVEQLTLTGTVTAA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHEEEHH
RRSPISSEIAGKIAQLQVEEGDRIPSGGVLATLDARRARLASRQAQAAMRQAEAELVDAE
HCCCCHHHHHCHHHEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLEMARRLAERRAVSEDERGTLETEVQTDRAVLERLRAEAGEQALLIDMHEIRAPFAGV
HHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHH
VAQRFTEAGAWVEPGDPVVELVDLDQLRIDVAAPQEFYPRLAGGARATVRLDAQPGSALE
HHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCC
GTLLSAVPVASPGSRTFLARIVVSNENAALMPGLSAKVTLHLPTGNRHPVIPRDALIRHP
CHHHHHCCCCCCCCCEEEEEEEEECCCCEECCCCCEEEEEEECCCCCCCCCCHHHHEECC
DGRTTAWVVVSAGGALTAQERNIVTGRAFAGRVEVRRGLSDGDSVVTEGNEGLQPGQAVE
CCCCEEEEEEECCCEEEECCCCEEECCHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHH
IVRNAS
HEECCC
>Mature Secondary Structure
MHSITLKTLLFTPVCSGLLLFLLSALPAYAQSGAARVSVAAVETEEIVEQLTLTGTVTAA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHEEEHH
RRSPISSEIAGKIAQLQVEEGDRIPSGGVLATLDARRARLASRQAQAAMRQAEAELVDAE
HCCCCHHHHHCHHHEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLEMARRLAERRAVSEDERGTLETEVQTDRAVLERLRAEAGEQALLIDMHEIRAPFAGV
HHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHH
VAQRFTEAGAWVEPGDPVVELVDLDQLRIDVAAPQEFYPRLAGGARATVRLDAQPGSALE
HHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCC
GTLLSAVPVASPGSRTFLARIVVSNENAALMPGLSAKVTLHLPTGNRHPVIPRDALIRHP
CHHHHHCCCCCCCCCEEEEEEEEECCCCEECCCCCEEEEEEECCCCCCCCCCHHHHEECC
DGRTTAWVVVSAGGALTAQERNIVTGRAFAGRVEVRRGLSDGDSVVTEGNEGLQPGQAVE
CCCCEEEEEEECCCEEEECCCCEEECCHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHH
IVRNAS
HEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]