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The map label for this gene is pitB [C]

Identifier: 114799704

GI number: 114799704

Start: 3210675

End: 3212183

Strand: Direct

Name: pitB [C]

Synonym: HNE_3040

Alternate gene names: 114799704

Gene position: 3210675-3212183 (Clockwise)

Preceding gene: 114798495

Following gene: 114798958

Centisome position: 86.66

GC content: 67.2

Gene sequence:

>1509_bases
ATGGACGGCAAGGAACCCACGATCGTCAAACAGACGCTCGACAAAGACCTTGCGAAGATCGGCAAAGCCGAAGCCGCCGT
CGCCACCACAGCGCGCAGCGTCGTCGCCCCCGGTCTCGCTTTCCTGTTCCTCGCGGCCATCGTGCTGGTTGGCGCCAGCC
TGTTCGGCAGCCAGAGCAACGGCCTCCTCATCGTCGCCGCCGCCGCCATCGGCGGCTACATGGCGCTCAACATCGGCGCC
AATGACGTGGCCAACAATGTCGGTCCCGCCGTCGGCTCCAAGGCCCTCACCATGACCGGCGCGATCATCATCGCCGCCAT
CTGCGAAAGCGCCGGCGCCCTGCTGGCAGGCGGCGATGTCGTCTCCACCATCTCCAAGGGCATCATCAGCCCCGAGAACG
TCTCCAACACCGAAACCTTCGTCTGGCTGATGATGGCCGCGCTGATCTCCGCCGCCCTCTGGATCAACCTCGCCACCGTC
CTGGGCGCGCCCGTCTCCACCACCCACTCCATCGTCGGCGGCGTCATGGGGGCCGGCATCGCCGCGGCCGGCTTCGGCGC
GGTCAGCTGGCCGGTGATGGCGGGCATTGCCGCCTCCTGGGTCGTCTCCCCCGTGCTGGGCGGCATCATCGCCGCCGCCG
TGCTCGCCTTCATCAAGATCGCCATCATCTACCGGGAAGACAAGATCGCCGCCGCCCGCCGCTGGGTGCCCGTGCTCATC
GGCGTCATGGCCGGCGCCTTCGGCACCTATATGGCGCTGAAAGGCGTCAGCAAGCTGATCGATATTTCCCTGCCCCATGC
CCTGCTGATTGGCCTCGGCATTGCCCTCGCCGCCTGGGGCATCACCCACCCGCTGATCCGGCGCCAGTCCGAAGGCATGG
AAAACCGCAACCAGTCCCTGCGCCAGCTGTTTATCTGGCCGCTGATCTTCTCCGCCGCCCTCCTCTCCTTCGCCCATGGC
GCCAACGATGTGGCCAACGCCATCGGCCCGCTGGCCGCCATCGTCCACGCCCTTCAGGATGGCGAAGTCGCCGCCAGCGT
CACCATTCCCCTCTGGGTCATGCTGATCGGCGCGATGGGCATTTCCTTCGGCCTCGTCCTCTTCGGGCCAAAGCTCATCC
GCATGGTCGGGGACTCGATCACCAAGCTCAATCCGATGCGCGCCTTCTGCGTCGCCCTCGCCGCCGCCGTCACCGTCATC
ATCGCCAGCTGGCTCGGCCTGCCCGTCTCCTCCACCCACATTGCCGTGGGCGCCATCTTCGGCGTCGGCTTCTTCCGCGA
ATGGTTCACCGCCAACTCGCACACGCGCCTGTCCTATGTGCACACCCGGTCGCCCAACGCGGCGGAGACCGAATTCTCCG
GCCTCGCCCGCCGCAAGTCGGCCCGCAAACTCGTCCGCCGCGCCCACGTCACCAGCATCGCCGCCGCCTGGATCATCACC
GTCCCCGCCAGCGCCACCCTCGCCGGCGGCATCTACCTGGCGCTACAACACCTCTTCAAGATCGTCTGA

Upstream 100 bases:

>100_bases
GACATCTTCAGTTGCAGGCCGGAATCTTGCCGCTCACGGCTTGAAAAGTTCGGGCGCTTGCGGCTGATGCGCCTCACATC
GCCTAAAGCGGGGGCTTCAT

Downstream 100 bases:

>100_bases
GGGCGGACAGCCCATCTTCACCATCCCAGCCCCTTGAATTCTCCCCTTGCGCGAAGGTTAACAAAGCGTAAAGGTAGAAA
TCCAAGAGGGGTACTATGGG

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MDGKEPTIVKQTLDKDLAKIGKAEAAVATTARSVVAPGLAFLFLAAIVLVGASLFGSQSNGLLIVAAAAIGGYMALNIGA
NDVANNVGPAVGSKALTMTGAIIIAAICESAGALLAGGDVVSTISKGIISPENVSNTETFVWLMMAALISAALWINLATV
LGAPVSTTHSIVGGVMGAGIAAAGFGAVSWPVMAGIAASWVVSPVLGGIIAAAVLAFIKIAIIYREDKIAAARRWVPVLI
GVMAGAFGTYMALKGVSKLIDISLPHALLIGLGIALAAWGITHPLIRRQSEGMENRNQSLRQLFIWPLIFSAALLSFAHG
ANDVANAIGPLAAIVHALQDGEVAASVTIPLWVMLIGAMGISFGLVLFGPKLIRMVGDSITKLNPMRAFCVALAAAVTVI
IASWLGLPVSSTHIAVGAIFGVGFFREWFTANSHTRLSYVHTRSPNAAETEFSGLARRKSARKLVRRAHVTSIAAAWIIT
VPASATLAGGIYLALQHLFKIV

Sequences:

>Translated_502_residues
MDGKEPTIVKQTLDKDLAKIGKAEAAVATTARSVVAPGLAFLFLAAIVLVGASLFGSQSNGLLIVAAAAIGGYMALNIGA
NDVANNVGPAVGSKALTMTGAIIIAAICESAGALLAGGDVVSTISKGIISPENVSNTETFVWLMMAALISAALWINLATV
LGAPVSTTHSIVGGVMGAGIAAAGFGAVSWPVMAGIAASWVVSPVLGGIIAAAVLAFIKIAIIYREDKIAAARRWVPVLI
GVMAGAFGTYMALKGVSKLIDISLPHALLIGLGIALAAWGITHPLIRRQSEGMENRNQSLRQLFIWPLIFSAALLSFAHG
ANDVANAIGPLAAIVHALQDGEVAASVTIPLWVMLIGAMGISFGLVLFGPKLIRMVGDSITKLNPMRAFCVALAAAVTVI
IASWLGLPVSSTHIAVGAIFGVGFFREWFTANSHTRLSYVHTRSPNAAETEFSGLARRKSARKLVRRAHVTSIAAAWIIT
VPASATLAGGIYLALQHLFKIV
>Mature_502_residues
MDGKEPTIVKQTLDKDLAKIGKAEAAVATTARSVVAPGLAFLFLAAIVLVGASLFGSQSNGLLIVAAAAIGGYMALNIGA
NDVANNVGPAVGSKALTMTGAIIIAAICESAGALLAGGDVVSTISKGIISPENVSNTETFVWLMMAALISAALWINLATV
LGAPVSTTHSIVGGVMGAGIAAAGFGAVSWPVMAGIAASWVVSPVLGGIIAAAVLAFIKIAIIYREDKIAAARRWVPVLI
GVMAGAFGTYMALKGVSKLIDISLPHALLIGLGIALAAWGITHPLIRRQSEGMENRNQSLRQLFIWPLIFSAALLSFAHG
ANDVANAIGPLAAIVHALQDGEVAASVTIPLWVMLIGAMGISFGLVLFGPKLIRMVGDSITKLNPMRAFCVALAAAVTVI
IASWLGLPVSSTHIAVGAIFGVGFFREWFTANSHTRLSYVHTRSPNAAETEFSGLARRKSARKLVRRAHVTSIAAAWIIT
VPASATLAGGIYLALQHLFKIV

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=237, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI31543630, Length=284, Percent_Identity=32.3943661971831, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25146401, Length=481, Percent_Identity=29.5218295218295, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32566716, Length=414, Percent_Identity=30.4347826086957, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71981576, Length=464, Percent_Identity=29.5258620689655, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17557328, Length=463, Percent_Identity=25.0539956803456, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17539280, Length=235, Percent_Identity=34.468085106383, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17536725, Length=147, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319773, Length=137, Percent_Identity=37.956204379562, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356511, Length=208, Percent_Identity=34.1346153846154, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 51741; Mature: 51741

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGKEPTIVKQTLDKDLAKIGKAEAAVATTARSVVAPGLAFLFLAAIVLVGASLFGSQSN
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLLIVAAAAIGGYMALNIGANDVANNVGPAVGSKALTMTGAIIIAAICESAGALLAGGDV
CEEEEEEHHHCCEEEEECCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEECCHH
VSTISKGIISPENVSNTETFVWLMMAALISAALWINLATVLGAPVSTTHSIVGGVMGAGI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAAGFGAVSWPVMAGIAASWVVSPVLGGIIAAAVLAFIKIAIIYREDKIAAARRWVPVLI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
GVMAGAFGTYMALKGVSKLIDISLPHALLIGLGIALAAWGITHPLIRRQSEGMENRNQSL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RQLFIWPLIFSAALLSFAHGANDVANAIGPLAAIVHALQDGEVAASVTIPLWVMLIGAMG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
ISFGLVLFGPKLIRMVGDSITKLNPMRAFCVALAAAVTVIIASWLGLPVSSTHIAVGAIF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GVGFFREWFTANSHTRLSYVHTRSPNAAETEFSGLARRKSARKLVRRAHVTSIAAAWIIT
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VPASATLAGGIYLALQHLFKIV
CCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDGKEPTIVKQTLDKDLAKIGKAEAAVATTARSVVAPGLAFLFLAAIVLVGASLFGSQSN
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLLIVAAAAIGGYMALNIGANDVANNVGPAVGSKALTMTGAIIIAAICESAGALLAGGDV
CEEEEEEHHHCCEEEEECCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEECCHH
VSTISKGIISPENVSNTETFVWLMMAALISAALWINLATVLGAPVSTTHSIVGGVMGAGI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAAGFGAVSWPVMAGIAASWVVSPVLGGIIAAAVLAFIKIAIIYREDKIAAARRWVPVLI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
GVMAGAFGTYMALKGVSKLIDISLPHALLIGLGIALAAWGITHPLIRRQSEGMENRNQSL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RQLFIWPLIFSAALLSFAHGANDVANAIGPLAAIVHALQDGEVAASVTIPLWVMLIGAMG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
ISFGLVLFGPKLIRMVGDSITKLNPMRAFCVALAAAVTVIIASWLGLPVSSTHIAVGAIF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GVGFFREWFTANSHTRLSYVHTRSPNAAETEFSGLARRKSARKLVRRAHVTSIAAAWIIT
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VPASATLAGGIYLALQHLFKIV
CCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]