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The map label for this gene is dacF [H]

Identifier: 114799690

GI number: 114799690

Start: 1860233

End: 1861369

Strand: Reverse

Name: dacF [H]

Synonym: HNE_1814

Alternate gene names: 114799690

Gene position: 1861369-1860233 (Counterclockwise)

Preceding gene: 114797813

Following gene: 114799318

Centisome position: 50.24

GC content: 60.07

Gene sequence:

>1137_bases
GTGTTTGCGGCCCTACTGACCCTCGGAGCGCTCAGCGGCGCCAGCGTATCGGCTCAGATGATCGACACGCCGGCGGAGTA
TGCTGTGATCATGGATCACGCGACCGGCAAGATCCTCTATTCCAAGCGCGGCGATGAGCCGATGACGCCCGCTTCGATGA
CCAAGATGATGACAGCCTTTGTCGTCTTCGAGCTGATCGACCGCGGCGAACTCTCGCTGACCGACAAGTTCACCGTCAGC
GAAGATGCGTGGCGGCGCGGCGGCTTCCCTTCCGGTACGTCCACGATGGGCCTGGTGCCCAAGGATACGCCGACGGTTGA
AGAACTGCTTCATGGCGTGATCACGATGTCCGGCAACGATGCCTGCATTGTGCTGGCGCAGGGCATTTCAGGGTCTGAAG
AAGCCTTCTCCCGCAGAATGACGGAAATGGCCCACGAGCTTGGGCTGTCTTCGGTCAATTTCGTCAACACGACCGGGCTT
GAGGGCGATGGACATGTCGTTTCCGCCGAAGACCTCGCCCGGCTCGCCAAGATGACGATCGACAAGTATCCGCAATATTA
TGAGTGGTATTCGCTGCCCTCTTATACCTGGCGCGAATACACGCAGGCCAACCGCAATCCTCTGCTCGAGGTTGCCGGGG
CCGATGGCCTCAAGACGGGGCATCTGGAATCTTCCGGCTATGGTCTGACAGCGTCGGCTGTGCGCGATGGCGTGCGGCGC
ACGATCGTCGTGAACGGGCTGCCGACCATGGCGGCGCGCGCGCAGGAGAGCGAACGCCTGATGCGCGTCGCCTTCCAGAG
CTTTGACCTCAAAACCATCGGGCCCGAAACGCTGGTATTGCCAGATGTGGAAGTGTGGCTGGGAGAGGCCCGTGCTGTTC
CGGTTTCGCTGGCAGAACCGATCGAAGTGGCCGGACACAAGGCTGCCTTTTCCAAAGCCAATGTCGAGATCGTTCTTGAT
GGGCCGGTGAAAGCGCCTCTGAAGAAGGGCGATCAGGTCGGCCGGCTGGTGATCACGATGGAAGGTTCTGAGCCTACCGA
AGCCCCGATTATCGCCGAGGCAGATGTGAAGAAGCTGGGATTTGTCGGCATGGCGCTTGAGGGTGTTCGCACGTTTATTT
CCAGCAGCAATGACTGA

Upstream 100 bases:

>100_bases
ACCGGCCCTTTTTCCTGCCTATACAGTGCAGAAGGCCGCTGAATATCCTGCGGCCACCTGAACGGCACAGGAGAAAGACA
TCGTGGTTCCCTTTCGCAAG

Downstream 100 bases:

>100_bases
TACGGCTGCTGGTCACTTCATCACGCTGGAAGGCGGTGAAGGCACCGGCAAATCGACGCTGGCCAAGGCGCTTGCCCGGA
AGCTGGAGGCCCACGGCATT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFAALLTLGALSGASVSAQMIDTPAEYAVIMDHATGKILYSKRGDEPMTPASMTKMMTAFVVFELIDRGELSLTDKFTVS
EDAWRRGGFPSGTSTMGLVPKDTPTVEELLHGVITMSGNDACIVLAQGISGSEEAFSRRMTEMAHELGLSSVNFVNTTGL
EGDGHVVSAEDLARLAKMTIDKYPQYYEWYSLPSYTWREYTQANRNPLLEVAGADGLKTGHLESSGYGLTASAVRDGVRR
TIVVNGLPTMAARAQESERLMRVAFQSFDLKTIGPETLVLPDVEVWLGEARAVPVSLAEPIEVAGHKAAFSKANVEIVLD
GPVKAPLKKGDQVGRLVITMEGSEPTEAPIIAEADVKKLGFVGMALEGVRTFISSSND

Sequences:

>Translated_378_residues
MFAALLTLGALSGASVSAQMIDTPAEYAVIMDHATGKILYSKRGDEPMTPASMTKMMTAFVVFELIDRGELSLTDKFTVS
EDAWRRGGFPSGTSTMGLVPKDTPTVEELLHGVITMSGNDACIVLAQGISGSEEAFSRRMTEMAHELGLSSVNFVNTTGL
EGDGHVVSAEDLARLAKMTIDKYPQYYEWYSLPSYTWREYTQANRNPLLEVAGADGLKTGHLESSGYGLTASAVRDGVRR
TIVVNGLPTMAARAQESERLMRVAFQSFDLKTIGPETLVLPDVEVWLGEARAVPVSLAEPIEVAGHKAAFSKANVEIVLD
GPVKAPLKKGDQVGRLVITMEGSEPTEAPIIAEADVKKLGFVGMALEGVRTFISSSND
>Mature_378_residues
MFAALLTLGALSGASVSAQMIDTPAEYAVIMDHATGKILYSKRGDEPMTPASMTKMMTAFVVFELIDRGELSLTDKFTVS
EDAWRRGGFPSGTSTMGLVPKDTPTVEELLHGVITMSGNDACIVLAQGISGSEEAFSRRMTEMAHELGLSSVNFVNTTGL
EGDGHVVSAEDLARLAKMTIDKYPQYYEWYSLPSYTWREYTQANRNPLLEVAGADGLKTGHLESSGYGLTASAVRDGVRR
TIVVNGLPTMAARAQESERLMRVAFQSFDLKTIGPETLVLPDVEVWLGEARAVPVSLAEPIEVAGHKAAFSKANVEIVLD
GPVKAPLKKGDQVGRLVITMEGSEPTEAPIIAEADVKKLGFVGMALEGVRTFISSSND

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=365, Percent_Identity=35.3424657534247, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786851, Length=354, Percent_Identity=30.7909604519774, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082022, Length=328, Percent_Identity=34.4512195121951, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 40614; Mature: 40614

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAALLTLGALSGASVSAQMIDTPAEYAVIMDHATGKILYSKRGDEPMTPASMTKMMTAF
CHHHHHHHHCCCCCCCCHHEECCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
VVFELIDRGELSLTDKFTVSEDAWRRGGFPSGTSTMGLVPKDTPTVEELLHGVITMSGND
HHHHHHCCCCEEEEECEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCC
ACIVLAQGISGSEEAFSRRMTEMAHELGLSSVNFVNTTGLEGDGHVVSAEDLARLAKMTI
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECHHHHHHHHHHHH
DKYPQYYEWYSLPSYTWREYTQANRNPLLEVAGADGLKTGHLESSGYGLTASAVRDGVRR
HHCCCHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHCCCEE
TIVVNGLPTMAARAQESERLMRVAFQSFDLKTIGPETLVLPDVEVWLGEARAVPVSLAEP
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCEEECCCHHHHHCCCCCCCCCCCCC
IEVAGHKAAFSKANVEIVLDGPVKAPLKKGDQVGRLVITMEGSEPTEAPIIAEADVKKLG
HHHCCCHHHHCCCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCHHHHH
FVGMALEGVRTFISSSND
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFAALLTLGALSGASVSAQMIDTPAEYAVIMDHATGKILYSKRGDEPMTPASMTKMMTAF
CHHHHHHHHCCCCCCCCHHEECCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
VVFELIDRGELSLTDKFTVSEDAWRRGGFPSGTSTMGLVPKDTPTVEELLHGVITMSGND
HHHHHHCCCCEEEEECEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCC
ACIVLAQGISGSEEAFSRRMTEMAHELGLSSVNFVNTTGLEGDGHVVSAEDLARLAKMTI
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECHHHHHHHHHHHH
DKYPQYYEWYSLPSYTWREYTQANRNPLLEVAGADGLKTGHLESSGYGLTASAVRDGVRR
HHCCCHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHCCCEE
TIVVNGLPTMAARAQESERLMRVAFQSFDLKTIGPETLVLPDVEVWLGEARAVPVSLAEP
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCEEECCCHHHHHCCCCCCCCCCCCC
IEVAGHKAAFSKANVEIVLDGPVKAPLKKGDQVGRLVITMEGSEPTEAPIIAEADVKKLG
HHHCCCHHHHCCCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCHHHHH
FVGMALEGVRTFISSSND
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]