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The map label for this gene is mdeA [H]

Identifier: 114799633

GI number: 114799633

Start: 3042809

End: 3044098

Strand: Direct

Name: mdeA [H]

Synonym: HNE_2885

Alternate gene names: 114799633

Gene position: 3042809-3044098 (Clockwise)

Preceding gene: 114798622

Following gene: 114797494

Centisome position: 82.13

GC content: 63.8

Gene sequence:

>1290_bases
ATGACTGACGGCGTATACAAAGACTTTGCCACCACCCAGATCCATGCCGGCTCCGCGCCAGACCCCGCAACGGGCGCGCG
CCAGGTGCCGATCTATCAGACCACGGCCTATGTGTTCCGCGACGCTGACCACGCCGCCGCCCTCTTCGGCCTGCAGGAAG
TCGGCTACATCTATTCCCGCCTCACCAACCCGACCATCGGCGCGCTTCAGGCCCGCCTTGCTGCGCTGGAAGGCGGCGCC
GGCGCGGTCTGCTGCTCCTCCGGCCATGCGGCCCAGATCATGGCGCTCTTCCCGCTGATGGGACCGGGCAAGAACATCGT
CGCCTCCACGCGCCTCTATGGCGGCACGATCACCCAGTTCACCCAGACCTTCAAACGCTTCGGCTGGTCGGCAAAGCTCA
TCGACACCGAAGACCTCGCCGCTGTCAAAGGCGCGATTGACGAGAACACCCGCGCCATCTTCTGCGAGACCATTGCCAAT
CCCGGCGGCTATATCAGCGACTTGGACGCGCTGGCAAAAATTGCCGATGAGGCGGGCATTCCCCTGATCGTCGACAACAC
AACCGCCACGCCCTATCTCTGCCGCCCGATCGAACATGGCGCCACGCTGGTCGTCCATTCGCTGACCAAATACATGACCG
GCAACGGCACCGTCACCGGCGGGGCCATCATCGATTCTGGCAAGTTCGACTGGTCGGCATCGGACAAATTCCCCTCCCTC
TCGGCGCCAGAGCCTGCCTATCAAGGCCTCAACTTCCATGAGACCTTCGGGCCCGCCGCCTTCACCTTCCACGGCATCGC
CATTGGCCTGCGCGACCTCGGCATGACCATGAACCCGCAAGGCGCCCATTACACGCTGATGGGCATCGAAACCCTCTCCC
TACGGATGGAAAAGCACGTTGCCAATGCGCAGAAGATCGCCGCCTGGCTGGAAACCCACCCGGCGGTGAAATCCGTCACC
TATCCAGGCCTCGCCTCCTCGCCGTATAAAGACCGCGCCGTGCGCATCACGCCCAAAGGCCCCGGCGCCCTCTTCACGGT
CCAGCTGAAGGGTGGCTATGACGCCTGCATGAAGTTCGTCGCCGCGCTGAAACTCTTCAGCCATGTGGCAAATCTCGGCG
ACGCGCGCAGCCTCGTCATCCATTCGGCCTCCACCACCCACCGTCAGCTGACGGATGAGCAGCAGATCGCCGCAGGCGCC
GCGCCAGACGTGGTGCGCCTCTCCATCGGCATCGAAGGCACAGAAGACCTGATCGCAGACCTCGACCAGGCCCTGAACGC
GGCAGGCTAG

Upstream 100 bases:

>100_bases
CCGGGGCTATGACAGCGGCGATCCAAACGTCTGCAACACCTCTAACTGATCCGGCTCCGGATCACACCACCTGACCAAAA
AACTAAAAGGGAGTAAGCCG

Downstream 100 bases:

>100_bases
AAAACCGGGAAAGGGCGGCTCTATCCGAGCCGCCCGACTCGCTCAATCAGCGCCTTTGCGGCGGCCGGATTGCGTTCCTT
GGCGCCCGCAATGAAGAAGG

Product: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase family protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTDGVYKDFATTQIHAGSAPDPATGARQVPIYQTTAYVFRDADHAAALFGLQEVGYIYSRLTNPTIGALQARLAALEGGA
GAVCCSSGHAAQIMALFPLMGPGKNIVASTRLYGGTITQFTQTFKRFGWSAKLIDTEDLAAVKGAIDENTRAIFCETIAN
PGGYISDLDALAKIADEAGIPLIVDNTTATPYLCRPIEHGATLVVHSLTKYMTGNGTVTGGAIIDSGKFDWSASDKFPSL
SAPEPAYQGLNFHETFGPAAFTFHGIAIGLRDLGMTMNPQGAHYTLMGIETLSLRMEKHVANAQKIAAWLETHPAVKSVT
YPGLASSPYKDRAVRITPKGPGALFTVQLKGGYDACMKFVAALKLFSHVANLGDARSLVIHSASTTHRQLTDEQQIAAGA
APDVVRLSIGIEGTEDLIADLDQALNAAG

Sequences:

>Translated_429_residues
MTDGVYKDFATTQIHAGSAPDPATGARQVPIYQTTAYVFRDADHAAALFGLQEVGYIYSRLTNPTIGALQARLAALEGGA
GAVCCSSGHAAQIMALFPLMGPGKNIVASTRLYGGTITQFTQTFKRFGWSAKLIDTEDLAAVKGAIDENTRAIFCETIAN
PGGYISDLDALAKIADEAGIPLIVDNTTATPYLCRPIEHGATLVVHSLTKYMTGNGTVTGGAIIDSGKFDWSASDKFPSL
SAPEPAYQGLNFHETFGPAAFTFHGIAIGLRDLGMTMNPQGAHYTLMGIETLSLRMEKHVANAQKIAAWLETHPAVKSVT
YPGLASSPYKDRAVRITPKGPGALFTVQLKGGYDACMKFVAALKLFSHVANLGDARSLVIHSASTTHRQLTDEQQIAAGA
APDVVRLSIGIEGTEDLIADLDQALNAAG
>Mature_428_residues
TDGVYKDFATTQIHAGSAPDPATGARQVPIYQTTAYVFRDADHAAALFGLQEVGYIYSRLTNPTIGALQARLAALEGGAG
AVCCSSGHAAQIMALFPLMGPGKNIVASTRLYGGTITQFTQTFKRFGWSAKLIDTEDLAAVKGAIDENTRAIFCETIANP
GGYISDLDALAKIADEAGIPLIVDNTTATPYLCRPIEHGATLVVHSLTKYMTGNGTVTGGAIIDSGKFDWSASDKFPSLS
APEPAYQGLNFHETFGPAAFTFHGIAIGLRDLGMTMNPQGAHYTLMGIETLSLRMEKHVANAQKIAAWLETHPAVKSVTY
PGLASSPYKDRAVRITPKGPGALFTVQLKGGYDACMKFVAALKLFSHVANLGDARSLVIHSASTTHRQLTDEQQIAAGAA
PDVVRLSIGIEGTEDLIADLDQALNAAG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=31.3679245283019, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=29.2452830188679, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=27.8959810874704, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1790375, Length=419, Percent_Identity=31.980906921241, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789383, Length=423, Percent_Identity=22.9314420803783, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=30.8433734939759, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=33.2552693208431, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=31.924882629108, Blast_Score=165, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=44.3155452436195, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6319307, Length=429, Percent_Identity=31.7016317016317, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=360, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=32.6241134751773, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45305; Mature: 45174

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGVYKDFATTQIHAGSAPDPATGARQVPIYQTTAYVFRDADHAAALFGLQEVGYIYSR
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTIGALQARLAALEGGAGAVCCSSGHAAQIMALFPLMGPGKNIVASTRLYGGTITQF
HCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECEECCCHHHHH
TQTFKRFGWSAKLIDTEDLAAVKGAIDENTRAIFCETIANPGGYISDLDALAKIADEAGI
HHHHHHHCCEEEEECCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCC
PLIVDNTTATPYLCRPIEHGATLVVHSLTKYMTGNGTVTGGAIIDSGKFDWSASDKFPSL
EEEEECCCCCCCEECCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCCCCCCCCCCC
SAPEPAYQGLNFHETFGPAAFTFHGIAIGLRDLGMTMNPQGAHYTLMGIETLSLRMEKHV
CCCCHHHCCCCCHHHCCCHHEEHHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ANAQKIAAWLETHPAVKSVTYPGLASSPYKDRAVRITPKGPGALFTVQLKGGYDACMKFV
HHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHH
AALKLFSHVANLGDARSLVIHSASTTHRQLTDEQQIAAGAAPDVVRLSIGIEGTEDLIAD
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECCCCHHHHHHH
LDQALNAAG
HHHHHHCCC
>Mature Secondary Structure 
TDGVYKDFATTQIHAGSAPDPATGARQVPIYQTTAYVFRDADHAAALFGLQEVGYIYSR
CCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTIGALQARLAALEGGAGAVCCSSGHAAQIMALFPLMGPGKNIVASTRLYGGTITQF
HCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECEECCCHHHHH
TQTFKRFGWSAKLIDTEDLAAVKGAIDENTRAIFCETIANPGGYISDLDALAKIADEAGI
HHHHHHHCCEEEEECCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCC
PLIVDNTTATPYLCRPIEHGATLVVHSLTKYMTGNGTVTGGAIIDSGKFDWSASDKFPSL
EEEEECCCCCCCEECCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCCCCCCCCCCC
SAPEPAYQGLNFHETFGPAAFTFHGIAIGLRDLGMTMNPQGAHYTLMGIETLSLRMEKHV
CCCCHHHCCCCCHHHCCCHHEEHHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ANAQKIAAWLETHPAVKSVTYPGLASSPYKDRAVRITPKGPGALFTVQLKGGYDACMKFV
HHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHH
AALKLFSHVANLGDARSLVIHSASTTHRQLTDEQQIAAGAAPDVVRLSIGIEGTEDLIAD
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECCCCHHHHHHH
LDQALNAAG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]