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The map label for this gene is carA

Identifier: 114797494

GI number: 114797494

Start: 3051313

End: 3052473

Strand: Direct

Name: carA

Synonym: HNE_2892

Alternate gene names: 114797494

Gene position: 3051313-3052473 (Clockwise)

Preceding gene: 114799633

Following gene: 114800074

Centisome position: 82.36

GC content: 63.39

Gene sequence:

>1161_bases
ATGGACTCAGCCACTGGTGCCTTGGCCCTTGAAGATGGTACGATTTTCGCGGGTTTCGGGGCTGGGGCTGCCGGTATCAA
AGTGGGGGAGCTCTGCTTCAACACGGCGATTACCGGCTATCAGGAAATACTGACAGACCCGTCTTATGCGTCCCAGCTGG
TGCTGTTTACCTTCCCGCATATCGGAAATGTCGGCGCAAATGCCGAAGATCACGAAGAATCCACCGTTGAAGCAGCCGAC
GCCGCCCGCGGCGCCATCTTCCGCGAGCCGATCACCGCGCCCTCCAACTGGCGCTCGACCGAAGATTTCGGCGTCTGGCT
CGCCCGGCGCGGCATCATCGGCCTCTCCGGCATCGATACGCGCGCGCTGACCAAGCGCATCCGCGAAAAAGGCATGCCCA
AGGCCGCCATCTGCCACAAGCCGGACGGAAAGATCGACTTTGACGCGCTTGTCGAACTCGCCCGCAGCTGGAATGGCCTC
GACGGGGCAGACCTTGCCGATGTCGTCGACCAGGACGCCCCTTTCGAGCATACCGAAGGTCTCTGGCAGGCCGCCACCGG
CCACCAGACCGCGCTCGCCAATGCCGAATTCCACGTCGCCGTCATCGATTTCGGCGTGAAGAAAAATATCCTGCGCAATC
TCGCCAGCGCCGGCGCCCGCGCCACGGTCGTCAATGGCAATGTCAGCGCTGAGGAAGTCCTCGCCCTGAAACCCGATGGC
ATCGTCTTCGCCAACGGCCCTGGCGACCCGGCCGCCACCTTCGAGCGTTCCGGCGAAATGATCAAAACCCTCGTCGCCTC
GGGCCTGCCCATCCTCGGCATCTGCCTTGGCCACCAGCTCCTTGGTCTCGCCCTCGGCGCCAAGACCAAAAAGATGGCGC
AGGGCCACCACGGCGCCAACCACCCCGTCAAGGACCTCGCCACCGGCAAGGTCGAGATCGTCTCGATGAACCACGGCTTC
ACGGTTGATCCCGACACGCTTCCCCCTGGCGTGGAGGAGAGCCACCGCTCCCTCTTCGACGGCACAAATTCCGGCCTCGC
GGTCAAAGGCAAACCGATCATCTCGGTCCAGCACCACCCCGAAGCCTCCCCCGGCCCGCAAGACAGCTTCTACCTCTTCA
CCCGCTTCGCGGGCCTGATGCGGGACTATCGCGCGAAATAA

Upstream 100 bases:

>100_bases
GCCCCAATAAGCGGCCGAGTTTTGACCAGACCGGTTAAAACGATGTCAACCAGACTGGCAAGCGGTGAGAGGTATATCTT
CCATGAGCAGGAATCAAGAT

Downstream 100 bases:

>100_bases
CAAAAGGCCGCCCAAGGGCGGCCTTTTTCTTTGCACGGACAGCACCGAGCCATCTAAATAAGCTTCGCCTCAGTCTTCGC
CCGAACCTCATCCTCAGTCA

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MDSATGALALEDGTIFAGFGAGAAGIKVGELCFNTAITGYQEILTDPSYASQLVLFTFPHIGNVGANAEDHEESTVEAAD
AARGAIFREPITAPSNWRSTEDFGVWLARRGIIGLSGIDTRALTKRIREKGMPKAAICHKPDGKIDFDALVELARSWNGL
DGADLADVVDQDAPFEHTEGLWQAATGHQTALANAEFHVAVIDFGVKKNILRNLASAGARATVVNGNVSAEEVLALKPDG
IVFANGPGDPAATFERSGEMIKTLVASGLPILGICLGHQLLGLALGAKTKKMAQGHHGANHPVKDLATGKVEIVSMNHGF
TVDPDTLPPGVEESHRSLFDGTNSGLAVKGKPIISVQHHPEASPGPQDSFYLFTRFAGLMRDYRAK

Sequences:

>Translated_386_residues
MDSATGALALEDGTIFAGFGAGAAGIKVGELCFNTAITGYQEILTDPSYASQLVLFTFPHIGNVGANAEDHEESTVEAAD
AARGAIFREPITAPSNWRSTEDFGVWLARRGIIGLSGIDTRALTKRIREKGMPKAAICHKPDGKIDFDALVELARSWNGL
DGADLADVVDQDAPFEHTEGLWQAATGHQTALANAEFHVAVIDFGVKKNILRNLASAGARATVVNGNVSAEEVLALKPDG
IVFANGPGDPAATFERSGEMIKTLVASGLPILGICLGHQLLGLALGAKTKKMAQGHHGANHPVKDLATGKVEIVSMNHGF
TVDPDTLPPGVEESHRSLFDGTNSGLAVKGKPIISVQHHPEASPGPQDSFYLFTRFAGLMRDYRAK
>Mature_386_residues
MDSATGALALEDGTIFAGFGAGAAGIKVGELCFNTAITGYQEILTDPSYASQLVLFTFPHIGNVGANAEDHEESTVEAAD
AARGAIFREPITAPSNWRSTEDFGVWLARRGIIGLSGIDTRALTKRIREKGMPKAAICHKPDGKIDFDALVELARSWNGL
DGADLADVVDQDAPFEHTEGLWQAATGHQTALANAEFHVAVIDFGVKKNILRNLASAGARATVVNGNVSAEEVLALKPDG
IVFANGPGDPAATFERSGEMIKTLVASGLPILGICLGHQLLGLALGAKTKKMAQGHHGANHPVKDLATGKVEIVSMNHGF
TVDPDTLPPGVEESHRSLFDGTNSGLAVKGKPIISVQHHPEASPGPQDSFYLFTRFAGLMRDYRAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=34.4647519582245, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI169790915, Length=398, Percent_Identity=31.9095477386935, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI21361331, Length=398, Percent_Identity=31.9095477386935, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=48.2939632545932, Blast_Score=339, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=36.9230769230769, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=400, Percent_Identity=34.75, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=31.5656565656566, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45555749, Length=398, Percent_Identity=33.6683417085427, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24642586, Length=398, Percent_Identity=33.6683417085427, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_HYPNA (Q0BY73)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761570.1
- ProteinModelPortal:   Q0BY73
- STRING:   Q0BY73
- GeneID:   4289709
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2892
- NMPDR:   fig|228405.5.peg.2771
- TIGR:   HNE_2892
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   Q0BY73
- ProtClustDB:   CLSK2531667
- BioCyc:   HNEP81032:HNE_2892-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40747; Mature: 40747

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 275-275 ACT_SITE 359-359 ACT_SITE 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSATGALALEDGTIFAGFGAGAAGIKVGELCFNTAITGYQEILTDPSYASQLVLFTFPH
CCCCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCC
IGNVGANAEDHEESTVEAADAARGAIFREPITAPSNWRSTEDFGVWLARRGIIGLSGIDT
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCH
RALTKRIREKGMPKAAICHKPDGKIDFDALVELARSWNGLDGADLADVVDQDAPFEHTEG
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHH
LWQAATGHQTALANAEFHVAVIDFGVKKNILRNLASAGARATVVNGNVSAEEVLALKPDG
HHHHCCCCCEEECCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEECCCC
IVFANGPGDPAATFERSGEMIKTLVASGLPILGICLGHQLLGLALGAKTKKMAQGHHGAN
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
HPVKDLATGKVEIVSMNHGFTVDPDTLPPGVEESHRSLFDGTNSGLAVKGKPIISVQHHP
CCHHHHCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHCCCCCCEEECCCEEEEEECCC
EASPGPQDSFYLFTRFAGLMRDYRAK
CCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSATGALALEDGTIFAGFGAGAAGIKVGELCFNTAITGYQEILTDPSYASQLVLFTFPH
CCCCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCC
IGNVGANAEDHEESTVEAADAARGAIFREPITAPSNWRSTEDFGVWLARRGIIGLSGIDT
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCH
RALTKRIREKGMPKAAICHKPDGKIDFDALVELARSWNGLDGADLADVVDQDAPFEHTEG
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHH
LWQAATGHQTALANAEFHVAVIDFGVKKNILRNLASAGARATVVNGNVSAEEVLALKPDG
HHHHCCCCCEEECCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEECCCC
IVFANGPGDPAATFERSGEMIKTLVASGLPILGICLGHQLLGLALGAKTKKMAQGHHGAN
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
HPVKDLATGKVEIVSMNHGFTVDPDTLPPGVEESHRSLFDGTNSGLAVKGKPIISVQHHP
CCHHHHCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHCCCCCCEEECCCEEEEEECCC
EASPGPQDSFYLFTRFAGLMRDYRAK
CCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA