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The map label for this gene is gltX [H]

Identifier: 114799552

GI number: 114799552

Start: 3615111

End: 3615956

Strand: Direct

Name: gltX [H]

Synonym: HNE_3481

Alternate gene names: 114799552

Gene position: 3615111-3615956 (Clockwise)

Preceding gene: 114797543

Following gene: 114797206

Centisome position: 97.57

GC content: 63.0

Gene sequence:

>846_bases
ATGTCTGACTATATCACGCGTTTCGCGCCGTCACCCACCGGCCGCCTTCATCTTGGGCATGCCGTGTCGGCTTTCAATGT
CTGGGCTGCGGCCGCCGAGGCAGGCGGCAAGGTTTACCTTCGAATTGAAGACATCGACCAGACCCGCTGCAAAATCGAGT
TTGAGGCCGGAATTCTGGAAGACCTCGCCTGGATGGGGCTCTCCTGGGACGGTCCGGTCCGGCGTCAATCAGAGCATTTT
TCAGAGTATAGTGCCGTTCTGGACACACTCCGTGCCAGGGGGTTGATCTATCGCTGCTTTCGGACACGGCGTGAAATTGC
CTCCAGCCTGCCCGCCGGGGCTGATCCCGACGAGACGGGCTTTGTCAGCCGCCCGCTTTCACCCACCGAAGAGGCCGCAC
GCCTAGCCCACAATGCCGCCTTTGCCTGGCGCCTGTCTCTCGCCGCTGCCCGCGCGGCGCTCGGCGATGCCTGGCGCGCC
CTCCACTATACGGAAGAAACGCCGATAGGCCTGCGCCGCGTCCCGGCTGACCCCTCCCGCTTCGGAGATGTCGTGCTGGC
GCGCAAGGACACCCCTGCCAGCTATCATCTGGCCTGCTGCCATGATGATGCCTTGCAGGGCATTACCCATGTCGTGCGCG
GCGAAGACCTGCGCGCCGTCACCGCCATCCACGCCCTTCTTCAGACGCTCATGGGCTGGCCCCAGCCCGTCTATCGTTTT
CATCCGCTGTTGCTGGGGCCGGACGGAAAGAAACTTTCCAAACGCAATGCTGACAAAAGTCTGGCTGCCTGGCGCGCAGA
GGGGATGACGCCGGAAGATATTCGGGCCATGACAGCGCCCGCATGA

Upstream 100 bases:

>100_bases
GCCCATCCTGAGATCGACATCAGAGTTTACCTTATCTGGTTCAAGGCGATACGTTTCCTGCCAGTTCCTTGCAACAGGGA
AACGCCGCTAAAGGACGCCG

Downstream 100 bases:

>100_bases
CCCAGACACCCCCGGCCACAGCGCCCCTCCCAGTTTCCCTGCCCCCGGCCCGCAGCTGGATTGGCCCGCTTATGGTGCTG
GGCGGCGGCGTTGCCATAGG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSDYITRFAPSPTGRLHLGHAVSAFNVWAAAAEAGGKVYLRIEDIDQTRCKIEFEAGILEDLAWMGLSWDGPVRRQSEHF
SEYSAVLDTLRARGLIYRCFRTRREIASSLPAGADPDETGFVSRPLSPTEEAARLAHNAAFAWRLSLAAARAALGDAWRA
LHYTEETPIGLRRVPADPSRFGDVVLARKDTPASYHLACCHDDALQGITHVVRGEDLRAVTAIHALLQTLMGWPQPVYRF
HPLLLGPDGKKLSKRNADKSLAAWRAEGMTPEDIRAMTAPA

Sequences:

>Translated_281_residues
MSDYITRFAPSPTGRLHLGHAVSAFNVWAAAAEAGGKVYLRIEDIDQTRCKIEFEAGILEDLAWMGLSWDGPVRRQSEHF
SEYSAVLDTLRARGLIYRCFRTRREIASSLPAGADPDETGFVSRPLSPTEEAARLAHNAAFAWRLSLAAARAALGDAWRA
LHYTEETPIGLRRVPADPSRFGDVVLARKDTPASYHLACCHDDALQGITHVVRGEDLRAVTAIHALLQTLMGWPQPVYRF
HPLLLGPDGKKLSKRNADKSLAAWRAEGMTPEDIRAMTAPA
>Mature_280_residues
SDYITRFAPSPTGRLHLGHAVSAFNVWAAAAEAGGKVYLRIEDIDQTRCKIEFEAGILEDLAWMGLSWDGPVRRQSEHFS
EYSAVLDTLRARGLIYRCFRTRREIASSLPAGADPDETGFVSRPLSPTEEAARLAHNAAFAWRLSLAAARAALGDAWRAL
HYTEETPIGLRRVPADPSRFGDVVLARKDTPASYHLACCHDDALQGITHVVRGEDLRAVTAIHALLQTLMGWPQPVYRFH
PLLLGPDGKKLSKRNADKSLAAWRAEGMTPEDIRAMTAPA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=280, Percent_Identity=32.5, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788743, Length=271, Percent_Identity=31.3653136531365, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=34.3629343629344, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786895, Length=205, Percent_Identity=28.780487804878, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324540, Length=276, Percent_Identity=34.0579710144928, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24665321, Length=281, Percent_Identity=34.5195729537367, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 30979; Mature: 30848

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00435 PEROXIDASE_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYITRFAPSPTGRLHLGHAVSAFNVWAAAAEAGGKVYLRIEDIDQTRCKIEFEAGILE
CCCCHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHH
DLAWMGLSWDGPVRRQSEHFSEYSAVLDTLRARGLIYRCFRTRREIASSLPAGADPDETG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FVSRPLSPTEEAARLAHNAAFAWRLSLAAARAALGDAWRALHYTEETPIGLRRVPADPSR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHH
FGDVVLARKDTPASYHLACCHDDALQGITHVVRGEDLRAVTAIHALLQTLMGWPQPVYRF
HCCEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
HPLLLGPDGKKLSKRNADKSLAAWRAEGMTPEDIRAMTAPA
CCEEECCCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SDYITRFAPSPTGRLHLGHAVSAFNVWAAAAEAGGKVYLRIEDIDQTRCKIEFEAGILE
CCCHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHH
DLAWMGLSWDGPVRRQSEHFSEYSAVLDTLRARGLIYRCFRTRREIASSLPAGADPDETG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FVSRPLSPTEEAARLAHNAAFAWRLSLAAARAALGDAWRALHYTEETPIGLRRVPADPSR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHH
FGDVVLARKDTPASYHLACCHDDALQGITHVVRGEDLRAVTAIHALLQTLMGWPQPVYRF
HCCEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
HPLLLGPDGKKLSKRNADKSLAAWRAEGMTPEDIRAMTAPA
CCEEECCCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]