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The map label for this gene is mutB [H]

Identifier: 114799394

GI number: 114799394

Start: 1358128

End: 1360290

Strand: Reverse

Name: mutB [H]

Synonym: HNE_1326

Alternate gene names: 114799394

Gene position: 1360290-1358128 (Counterclockwise)

Preceding gene: 114798788

Following gene: 114800163

Centisome position: 36.71

GC content: 62.14

Gene sequence:

>2163_bases
ATGACCAAGTTCCCCGATTTCACGAAACTCGATCTGGCGACGCCAAGCACAAAATCCGGCACGCCGAAACTTGGCGAAGC
CTGGGACACGCCCGAAGGCATCGCGGTCAAATCCGCTTACACCGCCGCCGACAGCGCGGGGCTGGATTTCCTCGACGACT
ATCCGGGCCTTACGCCCTTCGGACGCGGCCCCTACCCTACGATGTATACCAACCAGCCCTGGACAATCCGTCAGTATGCG
GGCTTTTCAACGGCTGAAGACTCCAACGCCTTCTACCGGCGCAATCTGGCTGCCGGCCAAAAGGGTCTCTCGGTAGCATT
CGATCTCGCCACCCACCGGGGCTATGATTCCGATCACCCGCGCGTGACAGGCGATGTCGGCATGGCGGGCGTCGCCATCG
ACTCCATTCTCGACATGCGCACGCTGTTCTCCGGCATTCCGCTGGATCAGATGTCCGTCTCGATGACGATGAATGGCGCG
GTCCTGCCCATCCTAGCGCTCTACATTGCAGCCGCCGAAGAACAGGGCGTCACGCCTGACAAGCTGGCCGGCACCATTCA
GAATGACATTCTGAAAGAGTTCATGGTGCGGAACACCTATATCTATCCGCCCAAGCCCTCGATGCGGATCATTTCGGACA
TCTTCGCCTACACGTCCGAAAACATGCCGAAATACAACTCCATCTCGATCTCCGGCTATCACATGCAGGAAGCGGGCGCG
ACGGCAGACCTGGAACTTGCCTATACGCTGGCCGACGGCATCGAATATATCCGCGCCGGTGTTGCCGCCGGCCTGGATGT
CGACAGGTTTGCCCCGCGTCTCTCCTTCTTCTGGGCGATCGGCATGAACACGTTCATGGAAGTCGCCAAGATGCGTGCCG
CGCGCCTCATCTGGGCAAAGCTCGTCAAAGAGAAGTTCAATCCGAAGTCTGACAAATCCCTCAGCCTGCGGACACACTCG
CAGACCTCCGGCTGGAGTCTCACCGCGCAGGACGTTTACAACAACGTTATCCGTACCTGCCTGGAGGCCATCGCAGCCTC
GGGCGGCCAGACGCAAAGCCTGCACACCAACAGTTTCGACGAAGCTCTTGCCCTGCCGACGGATTTCTCCGCCCGCATCG
CGCGCAACACGCAGATCCTGCTGCAACAGGAAGCGGGCGCCTGCCAGTCGATCGACCTCTTCGGCGGCGCCTACTATGTG
GAACGCCTGACGCATGACCTGGCCGCCAAGGCGCTGAAACATATCGAGGAAGTCGAAGCGATGGGCGGCATGGCCAAAGC
CATCGAGGAAGGCCTGCCCAAGCTGCGTATCGAGGAAGCCGCCGCCAATACGCAGGCGCGCATCGACAGCGGCACGCAGA
CCGTTGTGGGCGTCAACAAATTCCTGCTGGACGAGGACGAAGATGTCCCTGTGTTGAAGGTTGACAACGCTGCCGTCCGC
CGCATGCAGCTTGAAAAGCTCGCGCGCCTGAAATCGGAACGTAACGAAGACGAAGTCACTGCAAAACTCGACGCCATCGC
CGAAGCTGCCGCAGGCACCAAGGGCAACCTTCTGGCCCTCGCCGTGGACGCGGCGCGCGCCAAGGCGACCGTGGGTGAAA
TTTCCGAAGCCGTCGAGCGCAGCCAGGGCCGCCACAGCGCCGTCATCCGCTCCATCAAAGGCGTCTATGGCGGCGGCGTG
AAAGGCAATGCAAAAGCTGAACACGCCCAGGCGCTCGCCTCGGACTTTGAAAAGACCAACGGCCGCCGGCCGAAGATCTA
CATCGCCAAGATGGGCCAGGACGGACATGACCGTGGACAGAAGGTCGTCGCCTCCGCGCTGATGGACCTTGGCTGGGAGG
TCGAGATCGGGCCCCTCTTCCAGACCCCGGAAGAAGCAGCCCGCGACGCCCGCGAAGCCGGGGTCGACATCGTCGCCGCC
TCCTCGCTCGCCGCTGGCCACCTGACGCTGGTGCCTGAACTCAAACGCGCCCTCGGCAACGAAGGCGCTGCCAACGCCCG
GATCATCGTGGGCGGCGTTATCCCGCCACAGGACTTCGACGCCCTGCGTACAGCCGGCGCCGCCGCGATCTTCCCACCGG
GCACAGTGATCGCCGACAGCGCCATCCGTATGCTGGAACTGCTTCTGGGAACGGATGATCCGGCGCGGACAGCAGCGGAG
TAG

Upstream 100 bases:

>100_bases
CCGGAAAATCAGAAGCCCCCGGCATTGATCTCAACATCTTCGCAGGCTGTGATGTGATCCATGCCCTGCAACTTGCCCAT
GCTGAGCTGGGAGTTTCCAA

Downstream 100 bases:

>100_bases
GTTTCAAGCGGAGAGCCCCATGATCCTCACCACCACACACACGGTTCAAGGCCGGGAAATCCGGGAATACAAAGGCATCG
TGTGCGGCGAGGTCGTGATC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTKFPDFTKLDLATPSTKSGTPKLGEAWDTPEGIAVKSAYTAADSAGLDFLDDYPGLTPFGRGPYPTMYTNQPWTIRQYA
GFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVTGDVGMAGVAIDSILDMRTLFSGIPLDQMSVSMTMNGA
VLPILALYIAAAEEQGVTPDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIFAYTSENMPKYNSISISGYHMQEAGA
TADLELAYTLADGIEYIRAGVAAGLDVDRFAPRLSFFWAIGMNTFMEVAKMRAARLIWAKLVKEKFNPKSDKSLSLRTHS
QTSGWSLTAQDVYNNVIRTCLEAIAASGGQTQSLHTNSFDEALALPTDFSARIARNTQILLQQEAGACQSIDLFGGAYYV
ERLTHDLAAKALKHIEEVEAMGGMAKAIEEGLPKLRIEEAAANTQARIDSGTQTVVGVNKFLLDEDEDVPVLKVDNAAVR
RMQLEKLARLKSERNEDEVTAKLDAIAEAAAGTKGNLLALAVDAARAKATVGEISEAVERSQGRHSAVIRSIKGVYGGGV
KGNAKAEHAQALASDFEKTNGRRPKIYIAKMGQDGHDRGQKVVASALMDLGWEVEIGPLFQTPEEAARDAREAGVDIVAA
SSLAAGHLTLVPELKRALGNEGAANARIIVGGVIPPQDFDALRTAGAAAIFPPGTVIADSAIRMLELLLGTDDPARTAAE

Sequences:

>Translated_720_residues
MTKFPDFTKLDLATPSTKSGTPKLGEAWDTPEGIAVKSAYTAADSAGLDFLDDYPGLTPFGRGPYPTMYTNQPWTIRQYA
GFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVTGDVGMAGVAIDSILDMRTLFSGIPLDQMSVSMTMNGA
VLPILALYIAAAEEQGVTPDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIFAYTSENMPKYNSISISGYHMQEAGA
TADLELAYTLADGIEYIRAGVAAGLDVDRFAPRLSFFWAIGMNTFMEVAKMRAARLIWAKLVKEKFNPKSDKSLSLRTHS
QTSGWSLTAQDVYNNVIRTCLEAIAASGGQTQSLHTNSFDEALALPTDFSARIARNTQILLQQEAGACQSIDLFGGAYYV
ERLTHDLAAKALKHIEEVEAMGGMAKAIEEGLPKLRIEEAAANTQARIDSGTQTVVGVNKFLLDEDEDVPVLKVDNAAVR
RMQLEKLARLKSERNEDEVTAKLDAIAEAAAGTKGNLLALAVDAARAKATVGEISEAVERSQGRHSAVIRSIKGVYGGGV
KGNAKAEHAQALASDFEKTNGRRPKIYIAKMGQDGHDRGQKVVASALMDLGWEVEIGPLFQTPEEAARDAREAGVDIVAA
SSLAAGHLTLVPELKRALGNEGAANARIIVGGVIPPQDFDALRTAGAAAIFPPGTVIADSAIRMLELLLGTDDPARTAAE
>Mature_719_residues
TKFPDFTKLDLATPSTKSGTPKLGEAWDTPEGIAVKSAYTAADSAGLDFLDDYPGLTPFGRGPYPTMYTNQPWTIRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVTGDVGMAGVAIDSILDMRTLFSGIPLDQMSVSMTMNGAV
LPILALYIAAAEEQGVTPDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIFAYTSENMPKYNSISISGYHMQEAGAT
ADLELAYTLADGIEYIRAGVAAGLDVDRFAPRLSFFWAIGMNTFMEVAKMRAARLIWAKLVKEKFNPKSDKSLSLRTHSQ
TSGWSLTAQDVYNNVIRTCLEAIAASGGQTQSLHTNSFDEALALPTDFSARIARNTQILLQQEAGACQSIDLFGGAYYVE
RLTHDLAAKALKHIEEVEAMGGMAKAIEEGLPKLRIEEAAANTQARIDSGTQTVVGVNKFLLDEDEDVPVLKVDNAAVRR
MQLEKLARLKSERNEDEVTAKLDAIAEAAAGTKGNLLALAVDAARAKATVGEISEAVERSQGRHSAVIRSIKGVYGGGVK
GNAKAEHAQALASDFEKTNGRRPKIYIAKMGQDGHDRGQKVVASALMDLGWEVEIGPLFQTPEEAARDAREAGVDIVAAS
SLAAGHLTLVPELKRALGNEGAANARIIVGGVIPPQDFDALRTAGAAAIFPPGTVIADSAIRMLELLLGTDDPARTAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=679, Percent_Identity=60.2356406480118, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=683, Percent_Identity=59.5900439238653, Blast_Score=801, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=682, Percent_Identity=60.9970674486804, Blast_Score=862, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 77297; Mature: 77166

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFPDFTKLDLATPSTKSGTPKLGEAWDTPEGIAVKSAYTAADSAGLDFLDDYPGLTPF
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCHHHCCCCCCCC
GRGPYPTMYTNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHP
CCCCCCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCC
RVTGDVGMAGVAIDSILDMRTLFSGIPLDQMSVSMTMNGAVLPILALYIAAAEEQGVTPD
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHH
KLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIFAYTSENMPKYNSISISGYHMQEAGA
HHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECEEECCCCC
TADLELAYTLADGIEYIRAGVAAGLDVDRFAPRLSFFWAIGMNTFMEVAKMRAARLIWAK
CCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVKEKFNPKSDKSLSLRTHSQTSGWSLTAQDVYNNVIRTCLEAIAASGGQTQSLHTNSFD
HHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
EALALPTDFSARIARNTQILLQQEAGACQSIDLFGGAYYVERLTHDLAAKALKHIEEVEA
HHHCCCCCCHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGGMAKAIEEGLPKLRIEEAAANTQARIDSGTQTVVGVNKFLLDEDEDVPVLKVDNAAVR
HHHHHHHHHHCCCCEEEHHHHCCCHHHCCCCCEEEEEHHHHHCCCCCCCCEEEECHHHHH
RMQLEKLARLKSERNEDEVTAKLDAIAEAAAGTKGNLLALAVDAARAKATVGEISEAVER
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
SQGRHSAVIRSIKGVYGGGVKGNAKAEHAQALASDFEKTNGRRPKIYIAKMGQDGHDRGQ
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
KVVASALMDLGWEVEIGPLFQTPEEAARDAREAGVDIVAASSLAAGHLTLVPELKRALGN
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEHHHHHHHCCC
EGAANARIIVGGVIPPQDFDALRTAGAAAIFPPGTVIADSAIRMLELLLGTDDPARTAAE
CCCCCCEEEEECCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
TKFPDFTKLDLATPSTKSGTPKLGEAWDTPEGIAVKSAYTAADSAGLDFLDDYPGLTPF
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCHHHCCCCCCCC
GRGPYPTMYTNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHP
CCCCCCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCC
RVTGDVGMAGVAIDSILDMRTLFSGIPLDQMSVSMTMNGAVLPILALYIAAAEEQGVTPD
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHH
KLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIFAYTSENMPKYNSISISGYHMQEAGA
HHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECEEECCCCC
TADLELAYTLADGIEYIRAGVAAGLDVDRFAPRLSFFWAIGMNTFMEVAKMRAARLIWAK
CCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVKEKFNPKSDKSLSLRTHSQTSGWSLTAQDVYNNVIRTCLEAIAASGGQTQSLHTNSFD
HHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
EALALPTDFSARIARNTQILLQQEAGACQSIDLFGGAYYVERLTHDLAAKALKHIEEVEA
HHHCCCCCCHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGGMAKAIEEGLPKLRIEEAAANTQARIDSGTQTVVGVNKFLLDEDEDVPVLKVDNAAVR
HHHHHHHHHHCCCCEEEHHHHCCCHHHCCCCCEEEEEHHHHHCCCCCCCCEEEECHHHHH
RMQLEKLARLKSERNEDEVTAKLDAIAEAAAGTKGNLLALAVDAARAKATVGEISEAVER
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
SQGRHSAVIRSIKGVYGGGVKGNAKAEHAQALASDFEKTNGRRPKIYIAKMGQDGHDRGQ
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
KVVASALMDLGWEVEIGPLFQTPEEAARDAREAGVDIVAASSLAAGHLTLVPELKRALGN
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEHHHHHHHCCC
EGAANARIIVGGVIPPQDFDALRTAGAAAIFPPGTVIADSAIRMLELLLGTDDPARTAAE
CCCCCCEEEEECCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]