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The map label for this gene is eda [H]

Identifier: 114799078

GI number: 114799078

Start: 1194085

End: 1194720

Strand: Direct

Name: eda [H]

Synonym: HNE_1187

Alternate gene names: 114799078

Gene position: 1194085-1194720 (Clockwise)

Preceding gene: 114799520

Following gene: 114799044

Centisome position: 32.23

GC content: 66.51

Gene sequence:

>636_bases
ATGTCCGATATTGTTCAGAAACTTGATGCCGCCGCAAAACAGGCGCCGGTTGTGCCGGTTCTTGTGGTGGACTCAGCCGC
TTCGGCTGGACCGCTGGCGAGCGCGCTGGAAGCGGCGGGCGTGACGATTGCGGAAGTGACGCTGCGCACCGCTGACGGGC
TGAAGGTTATCGAGGCGATGCGCAAGGCGGCGTCGGGGCTGATCGTCGGCGCGGGTACGGTACTCAGTGGGCGCGATGTT
GAGGCCGCGCTGAATGCGGGCGCGGAGTTTCTGGTGTCGCCCGGCATGTCGCCTGGGCTGCGCGCGGCGCTGGGCGGACG
CGAGCATCTGATGATCCCCGGCGTGGCAACCGCCAGCGAGGCGATGGCGCGGATGGAAGAGGGGTTTCAGCGGTTGAAGC
TGTTTCCGGCCGCTGTGGCGGGAGGTGTCCCGGCGCTGAAAGCGCTGGCCGGGCCGATGCCGCATCTGCGGTTCATGCCG
ACGGGCGGGATTACCGAAGATGAGGTGAAGGCGTATCTTGGCCAGCCGAACGTCTTTGCGGTGGGCGGTTCGTGGATCGC
AAGCCAGAGCGATATAGCGGCGGGCAACTGGGCGAAGATTACCGAGACCGCGCGGCGCCTGCTGGCAATGGGGTGA

Upstream 100 bases:

>100_bases
CGCTTGTCATTGGGGGCGACACGGGCCACAACAACGTTGACAGCCGTATGGGCGGGCTTTCGCCCCGCCGACCGCACTCC
TGACCTCCGGGGCCTTATCC

Downstream 100 bases:

>100_bases
GGCGCGGGCCTGCTGGCGGTGTCAGCGGGCGAATTTCTGCTTCACCTTTTGCGCCACATTGATCACCGACTGTTCGACGC
CTTCGGGCCGGCCGTGAATG

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSDIVQKLDAAAKQAPVVPVLVVDSAASAGPLASALEAAGVTIAEVTLRTADGLKVIEAMRKAASGLIVGAGTVLSGRDV
EAALNAGAEFLVSPGMSPGLRAALGGREHLMIPGVATASEAMARMEEGFQRLKLFPAAVAGGVPALKALAGPMPHLRFMP
TGGITEDEVKAYLGQPNVFAVGGSWIASQSDIAAGNWAKITETARRLLAMG

Sequences:

>Translated_211_residues
MSDIVQKLDAAAKQAPVVPVLVVDSAASAGPLASALEAAGVTIAEVTLRTADGLKVIEAMRKAASGLIVGAGTVLSGRDV
EAALNAGAEFLVSPGMSPGLRAALGGREHLMIPGVATASEAMARMEEGFQRLKLFPAAVAGGVPALKALAGPMPHLRFMP
TGGITEDEVKAYLGQPNVFAVGGSWIASQSDIAAGNWAKITETARRLLAMG
>Mature_210_residues
SDIVQKLDAAAKQAPVVPVLVVDSAASAGPLASALEAAGVTIAEVTLRTADGLKVIEAMRKAASGLIVGAGTVLSGRDVE
AALNAGAEFLVSPGMSPGLRAALGGREHLMIPGVATASEAMARMEEGFQRLKLFPAAVAGGVPALKALAGPMPHLRFMPT
GGITEDEVKAYLGQPNVFAVGGSWIASQSDIAAGNWAKITETARRLLAMG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=177, Percent_Identity=44.6327683615819, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 21352; Mature: 21221

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIVQKLDAAAKQAPVVPVLVVDSAASAGPLASALEAAGVTIAEVTLRTADGLKVIEAM
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
RKAASGLIVGAGTVLSGRDVEAALNAGAEFLVSPGMSPGLRAALGGREHLMIPGVATASE
HHHHCCEEEECCCEECCCCHHHHHHCCCCEEECCCCCCCHHHHHCCCCEEEECCCCHHHH
AMARMEEGFQRLKLFPAAVAGGVPALKALAGPMPHLRFMPTGGITEDEVKAYLGQPNVFA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEE
VGGSWIASQSDIAAGNWAKITETARRLLAMG
ECCCEECCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDIVQKLDAAAKQAPVVPVLVVDSAASAGPLASALEAAGVTIAEVTLRTADGLKVIEAM
HHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
RKAASGLIVGAGTVLSGRDVEAALNAGAEFLVSPGMSPGLRAALGGREHLMIPGVATASE
HHHHCCEEEECCCEECCCCHHHHHHCCCCEEECCCCCCCHHHHHCCCCEEEECCCCHHHH
AMARMEEGFQRLKLFPAAVAGGVPALKALAGPMPHLRFMPTGGITEDEVKAYLGQPNVFA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEE
VGGSWIASQSDIAAGNWAKITETARRLLAMG
ECCCEECCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]