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The map label for this gene is thiG [H]

Identifier: 114799032

GI number: 114799032

Start: 2349106

End: 2349903

Strand: Reverse

Name: thiG [H]

Synonym: HNE_2246

Alternate gene names: 114799032

Gene position: 2349903-2349106 (Counterclockwise)

Preceding gene: 114798481

Following gene: 114799358

Centisome position: 63.42

GC content: 64.04

Gene sequence:

>798_bases
GTGCCCGACACCGCCTCCCAGAGCCCCGTTGATGATCCGCTGGTCATCGCCGGAAAGACCTATGCCTCGCGCCTGATCGT
GGGCACCGGCAAATATGCCTCCTATGAGCAGAACGCCGATGCGGCGCGCGCCGCCGGGGCCGAGATCGTGACCGTGGCCT
TGCGCCGGGTGAATCTTTCGAATCCGAATGAGCCGCGTCTGACCGATCATGTGACGCCTGAGGAGTTCACCTTCCTGCCC
AACACTGCCGGTTGCTATACGGCCGATGAGGCCGTGCGCACCTTGCGCCTGGCGCGCGAGGCGGGCGGCTGGAACCTGGT
GAAGCTGGAAGTGCTGGCCGACCAGAAGACGCTTTATCCGAACATGCCCGAGACGCTGAAGGCGGCCGAAGCCCTCATCA
AGGATGGCTTCGAGGTGATGGTCTATTGTTCGGATGATCCGGTTTATGCGCGCCTGCTCGAAGAGGCGGGCTGCGTGGCG
ATCATGCCGCTCGGGTCGCTGATCGGGTCCGGCCTCGGGATCATGAACCCGGTGAACATCCGGCTGATCATTGAGGCCGC
GCGTGTGCCGGTGATTGTGGACGCGGGCGTGGGGACGGCATCGGACGCAGCGATTGCCATGGAGCTTGGATGTGACGGCG
TGTTGATGAACACGGCCATCGCGCAGGCGAAAGACCCGGTGCGGATGGCCCGCGCGATGAAGCATGCCGTGATTGCGGGG
CGCGAGGCGTATCTGGCCGGGCGGATGCCGAAGAAGCGTTATGCTGACCCGAGTTCACCTCTCGCGGGGCTTATATAA

Upstream 100 bases:

>100_bases
TCCAACGATTCCTCCAACGAGGGGCCGAAATCCTCCAAGAAAACTCCAACCACGCAGGCCTTGCGGCCGTTTTTCAAGTT
TCAGTCAGGAGACACCTCCC

Downstream 100 bases:

>100_bases
TCCCGTATGCAGACCAAACCTGACCTCATCATCTTCGACTTTGATGGCACGTTGGCGGACAGCGTGAGCTTCTTTCGTGC
GCTTTTGCCGGAGTTGTCTC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPDTASQSPVDDPLVIAGKTYASRLIVGTGKYASYEQNADAARAAGAEIVTVALRRVNLSNPNEPRLTDHVTPEEFTFLP
NTAGCYTADEAVRTLRLAREAGGWNLVKLEVLADQKTLYPNMPETLKAAEALIKDGFEVMVYCSDDPVYARLLEEAGCVA
IMPLGSLIGSGLGIMNPVNIRLIIEAARVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAQAKDPVRMARAMKHAVIAG
REAYLAGRMPKKRYADPSSPLAGLI

Sequences:

>Translated_265_residues
MPDTASQSPVDDPLVIAGKTYASRLIVGTGKYASYEQNADAARAAGAEIVTVALRRVNLSNPNEPRLTDHVTPEEFTFLP
NTAGCYTADEAVRTLRLAREAGGWNLVKLEVLADQKTLYPNMPETLKAAEALIKDGFEVMVYCSDDPVYARLLEEAGCVA
IMPLGSLIGSGLGIMNPVNIRLIIEAARVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAQAKDPVRMARAMKHAVIAG
REAYLAGRMPKKRYADPSSPLAGLI
>Mature_264_residues
PDTASQSPVDDPLVIAGKTYASRLIVGTGKYASYEQNADAARAAGAEIVTVALRRVNLSNPNEPRLTDHVTPEEFTFLPN
TAGCYTADEAVRTLRLAREAGGWNLVKLEVLADQKTLYPNMPETLKAAEALIKDGFEVMVYCSDDPVYARLLEEAGCVAI
MPLGSLIGSGLGIMNPVNIRLIIEAARVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAQAKDPVRMARAMKHAVIAGR
EAYLAGRMPKKRYADPSSPLAGLI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=48.015873015873, Blast_Score=220, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28127; Mature: 27996

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTASQSPVDDPLVIAGKTYASRLIVGTGKYASYEQNADAARAAGAEIVTVALRRVNLS
CCCCCCCCCCCCCEEEECCHHHCEEEEECCCCCCCCCCCCHHHHCCCHHEEHHHHHHCCC
NPNEPRLTDHVTPEEFTFLPNTAGCYTADEAVRTLRLAREAGGWNLVKLEVLADQKTLYP
CCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHCCC
NMPETLKAAEALIKDGFEVMVYCSDDPVYARLLEEAGCVAIMPLGSLIGSGLGIMNPVNI
CCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCEE
RLIIEAARVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAQAKDPVRMARAMKHAVIAG
EEEEEECCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCHHHHHHHHHHHHHCC
REAYLAGRMPKKRYADPSSPLAGLI
CHHHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PDTASQSPVDDPLVIAGKTYASRLIVGTGKYASYEQNADAARAAGAEIVTVALRRVNLS
CCCCCCCCCCCCEEEECCHHHCEEEEECCCCCCCCCCCCHHHHCCCHHEEHHHHHHCCC
NPNEPRLTDHVTPEEFTFLPNTAGCYTADEAVRTLRLAREAGGWNLVKLEVLADQKTLYP
CCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHCCC
NMPETLKAAEALIKDGFEVMVYCSDDPVYARLLEEAGCVAIMPLGSLIGSGLGIMNPVNI
CCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCEE
RLIIEAARVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAQAKDPVRMARAMKHAVIAG
EEEEEECCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCHHHHHHHHHHHHHCC
REAYLAGRMPKKRYADPSSPLAGLI
CHHHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA