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The map label for this gene is purC

Identifier: 114798983

GI number: 114798983

Start: 2766786

End: 2767565

Strand: Direct

Name: purC

Synonym: HNE_2605

Alternate gene names: 114798983

Gene position: 2766786-2767565 (Clockwise)

Preceding gene: 114798350

Following gene: 114799324

Centisome position: 74.68

GC content: 60.0

Gene sequence:

>780_bases
ATGAACAAGCGCCGCGTCATCTACGAAGGAAAAGCGAAAATCCTCTATGAGGGCCCCGAACCGGGCACGCTGATCCAGTA
TTTCAAGGACGACACCACCGCGTTCGATGCGACCAAGAAGGCCGTGCTCGATGGTAAAGGGGTTCTGAACAACCGCATCA
GCGAGTTCATCATGACCCATCTGACGTCGGTTGGCGTGCCCAACCACTTCATACGCCGCCTCAATATGCGCGAGCAGCTG
GTCCGCAAGGTCGACATCATCCCGCTCGAAGTGGTCGTGCGCAACATCGCCGCAGGATCGCTTTCCACGCGCCTCGGAAT
TCCCGAAGGCCAGTCCCTTCCGCGCCCGCTGGTGGAGTTCTACTTCAAGAACGATGCCCTGCATGACCCCATGGTCTCCG
AGGAGCACATTGCCGCCTTTGGCTGGGCCACCGCCCAGGAATATGACGACATCATCGCCCTCGCCCTGCGCGTGAACGAT
TTCATGTCGGGTCTCTTCGCAGGCGCCGGCATCCGCCTGGTCGATTTCAAGATCGAGTTTGGCCGCTGGTTCGAGAATGA
CCACGACATGCCGCGCATCCTTCTGGCCGACGAAATCAGCCCGGACAGCTGCCGCCTCTGGGACGCCAAAACCGGCGAAA
AGATGGACAAGGACCGCTTCCGCCGCGACATGGGCGGCGTAACCGAGGCTTATGCCGAAGTTGCCCGCCGTCTCGGCATC
ATCCGGGAATCAGGCGAAGAGACCGACAACGTCATCCACTTCACCGGCGGGAGCAAATAA

Upstream 100 bases:

>100_bases
TCACGGGTTTCGCGTAACAGTCCGCGCTTCAGGGGAGCGGGGCGAATGATTCCCGTTGGCAACGCATTTCCCTACGAACG
CGCAGGAAAGGCCGGACGAG

Downstream 100 bases:

>100_bases
TGAAAGCGGTCGTTCACGTATCCCTTAAATCCGGCGTGCTGGACCCTCAGGGCAAGGCCGTTGCCGACACCCTCGCCCGC
ATGGGCTATAGCGAAGTCGG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNKRRVIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLDGKGVLNNRISEFIMTHLTSVGVPNHFIRRLNMREQL
VRKVDIIPLEVVVRNIAAGSLSTRLGIPEGQSLPRPLVEFYFKNDALHDPMVSEEHIAAFGWATAQEYDDIIALALRVND
FMSGLFAGAGIRLVDFKIEFGRWFENDHDMPRILLADEISPDSCRLWDAKTGEKMDKDRFRRDMGGVTEAYAEVARRLGI
IRESGEETDNVIHFTGGSK

Sequences:

>Translated_259_residues
MNKRRVIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLDGKGVLNNRISEFIMTHLTSVGVPNHFIRRLNMREQL
VRKVDIIPLEVVVRNIAAGSLSTRLGIPEGQSLPRPLVEFYFKNDALHDPMVSEEHIAAFGWATAQEYDDIIALALRVND
FMSGLFAGAGIRLVDFKIEFGRWFENDHDMPRILLADEISPDSCRLWDAKTGEKMDKDRFRRDMGGVTEAYAEVARRLGI
IRESGEETDNVIHFTGGSK
>Mature_259_residues
MNKRRVIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLDGKGVLNNRISEFIMTHLTSVGVPNHFIRRLNMREQL
VRKVDIIPLEVVVRNIAAGSLSTRLGIPEGQSLPRPLVEFYFKNDALHDPMVSEEHIAAFGWATAQEYDDIIALALRVND
FMSGLFAGAGIRLVDFKIEFGRWFENDHDMPRILLADEISPDSCRLWDAKTGEKMDKDRFRRDMGGVTEAYAEVARRLGI
IRESGEETDNVIHFTGGSK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=245, Percent_Identity=32.6530612244898, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI5453539, Length=245, Percent_Identity=32.6530612244898, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI119220559, Length=252, Percent_Identity=31.7460317460317, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1788820, Length=240, Percent_Identity=46.6666666666667, Blast_Score=225, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17531275, Length=234, Percent_Identity=32.4786324786325, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319326, Length=261, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI18860083, Length=241, Percent_Identity=33.195020746888, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24583917, Length=241, Percent_Identity=28.2157676348548, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_HYPNA (Q0BYZ9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761294.1
- ProteinModelPortal:   Q0BYZ9
- SMR:   Q0BYZ9
- STRING:   Q0BYZ9
- GeneID:   4288259
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2605
- NMPDR:   fig|228405.5.peg.2502
- TIGR:   HNE_2605
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- PhylomeDB:   Q0BYZ9
- BioCyc:   HNEP81032:HNE_2605-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 29291; Mature: 29291

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRRVIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLDGKGVLNNRISEFIMTH
CCCCEEEEECCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LTSVGVPNHFIRRLNMREQLVRKVDIIPLEVVVRNIAAGSLSTRLGIPEGQSLPRPLVEF
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHH
YFKNDALHDPMVSEEHIAAFGWATAQEYDDIIALALRVNDFMSGLFAGAGIRLVDFKIEF
HHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
GRWFENDHDMPRILLADEISPDSCRLWDAKTGEKMDKDRFRRDMGGVTEAYAEVARRLGI
CCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRESGEETDNVIHFTGGSK
HHCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNKRRVIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLDGKGVLNNRISEFIMTH
CCCCEEEEECCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LTSVGVPNHFIRRLNMREQLVRKVDIIPLEVVVRNIAAGSLSTRLGIPEGQSLPRPLVEF
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHH
YFKNDALHDPMVSEEHIAAFGWATAQEYDDIIALALRVNDFMSGLFAGAGIRLVDFKIEF
HHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
GRWFENDHDMPRILLADEISPDSCRLWDAKTGEKMDKDRFRRDMGGVTEAYAEVARRLGI
CCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRESGEETDNVIHFTGGSK
HHCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA