Click here to switch to the map view.

The map label for this gene is gltX1

Identifier: 114798943

GI number: 114798943

Start: 1827695

End: 1829155

Strand: Reverse

Name: gltX1

Synonym: HNE_1783

Alternate gene names: 114798943

Gene position: 1829155-1827695 (Counterclockwise)

Preceding gene: 114798535

Following gene: 114797964

Centisome position: 49.37

GC content: 58.93

Gene sequence:

>1461_bases
ATGTCCGTTGTTACTCGGTTTGCCCCATCCCCAACGGGAATGCTGCATATTGGAGGGGCTCGGACCGCACTTTTCAACTA
TTTGTTTGCGCGCCGGAATGGTGGCCGCTTTCTTCTGAGGATTGAGGATACGGATCGGGAGCGCTCCACCCAGGAGGCGA
CGGATGCAATTCTCGACGCCATGGAATGGCTTGGCCTGACACCCGACGAGCCACCGGTCATGCAATCTGCTCAAGTGGAC
CGGCACGCCGCCGTTGCCCATGACATGGTCGCGCGCGGCACGGCATTCCGCTGCTACGTGACGCCTGAGGAGCTCCAGGC
GCGCCGGGATCTTGGTGAAGAGAAGCGCCAGGCTGCAAAGCAGGAAGGTATCAGCGATGCCGAGAAAGAGGCGCTCCTGG
CAGAAGCCTCGCAACTGCTCGCTCCCTTCCGGTCGCCCTACAGGGATGGCGCCTCGCCGCCTTCACCGGATGCGCCCTTC
ACTGTTCGCCTCCGCGCACCGGAAAGCGGACCGCGCACCGTTGAAGATGGCGTGCAAGGCACCGTGACAATTGACGCCTC
CGAGATTGATGACCTCGTCATGCTCCGCGCCGATGGCACACCGACCTATATGCTCGCCGTCGTCGTTGATGATCACGACA
TGGGAATCACTCATGTCATCCGGGGCGACGATCACCTCCGGAACACCTTCCGCCAGGTGCCGATCTACGAGGCGATGGGC
TGGAGCGTTCCCAACTTCTCGCATGTGCCTATGATTCATGGAAACGACGGCGCAAAGCTGTCCAAGCGCCATGGCGCGCT
CTCTACCACGGCGTATCGCGACATGGGCTATCTGCCCGAGGCGATGAAAGCCTATCTCCTGCGCCTTGGCTGGAGCCATG
GGGATCAGGAGATATTCACGGATGAAGAGGCGGTCCAGGTTTTCGACGTAAGCGGCATCAACAAAGCGCCTGCCCGCCTG
GATCTGGACAAGCTGGCAACGGTGAATGCGCATTTCATGCGTCTGGCAGCAGACGAGCGGCTGTTTGACCTGATTTGTCC
AGTTTTGTCCAAGAATTGTTCGTTGAGTGACGCGGAAGTCGCTCGCATTCGCGCAGCTCTTCCCCACATGAAAGATCGCG
GCTCAACGCTCATTGAACTGGCCAACGCGTTCGCCTTCCTCTACGCAAAGCGGCCGCTCGAACTGAACAAAAATGCCGTT
AAAGCCTTGTCGGATGAAGGAAAACTTCGTCTTAAGGGACTATATGATGATCTGCAGCGAATGTCGCAGTGGTCAGGCGC
TAGCATTTCGGAAACGATAAAGTCATATTGCGCCGCAACAGGCCTATCGATGGGGCAGATAGGCCCGCCACTCAGAGCCG
CACTTACCGGCGGACTTCCAGCACCGGACCTTGCTCCGGTCATGGATTGGCTCGGCCGGGAAGAAACGCTCGCAAGAATT
GATGACCAACTCGCCGGCTGA

Upstream 100 bases:

>100_bases
GCTTCCGGAAGAAGCCGTCCCCCTCTTGCACTTCGCCCCGTCCTCGTGTTTGACCACCCCATCAGCGGCCACAGCGCCGG
TTAGTCGGAGCGTGCCACGG

Downstream 100 bases:

>100_bases
CACGCAAGCCAAAGCCGGCCAACAAACATGAACAGGGTTAGAGGCTGATGGAAAACAAGGTTAAAGACGCTGGTACGGCC
AAACTTGAAGTCGGCGGTAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSVVTRFAPSPTGMLHIGGARTALFNYLFARRNGGRFLLRIEDTDRERSTQEATDAILDAMEWLGLTPDEPPVMQSAQVD
RHAAVAHDMVARGTAFRCYVTPEELQARRDLGEEKRQAAKQEGISDAEKEALLAEASQLLAPFRSPYRDGASPPSPDAPF
TVRLRAPESGPRTVEDGVQGTVTIDASEIDDLVMLRADGTPTYMLAVVVDDHDMGITHVIRGDDHLRNTFRQVPIYEAMG
WSVPNFSHVPMIHGNDGAKLSKRHGALSTTAYRDMGYLPEAMKAYLLRLGWSHGDQEIFTDEEAVQVFDVSGINKAPARL
DLDKLATVNAHFMRLAADERLFDLICPVLSKNCSLSDAEVARIRAALPHMKDRGSTLIELANAFAFLYAKRPLELNKNAV
KALSDEGKLRLKGLYDDLQRMSQWSGASISETIKSYCAATGLSMGQIGPPLRAALTGGLPAPDLAPVMDWLGREETLARI
DDQLAG

Sequences:

>Translated_486_residues
MSVVTRFAPSPTGMLHIGGARTALFNYLFARRNGGRFLLRIEDTDRERSTQEATDAILDAMEWLGLTPDEPPVMQSAQVD
RHAAVAHDMVARGTAFRCYVTPEELQARRDLGEEKRQAAKQEGISDAEKEALLAEASQLLAPFRSPYRDGASPPSPDAPF
TVRLRAPESGPRTVEDGVQGTVTIDASEIDDLVMLRADGTPTYMLAVVVDDHDMGITHVIRGDDHLRNTFRQVPIYEAMG
WSVPNFSHVPMIHGNDGAKLSKRHGALSTTAYRDMGYLPEAMKAYLLRLGWSHGDQEIFTDEEAVQVFDVSGINKAPARL
DLDKLATVNAHFMRLAADERLFDLICPVLSKNCSLSDAEVARIRAALPHMKDRGSTLIELANAFAFLYAKRPLELNKNAV
KALSDEGKLRLKGLYDDLQRMSQWSGASISETIKSYCAATGLSMGQIGPPLRAALTGGLPAPDLAPVMDWLGREETLARI
DDQLAG
>Mature_485_residues
SVVTRFAPSPTGMLHIGGARTALFNYLFARRNGGRFLLRIEDTDRERSTQEATDAILDAMEWLGLTPDEPPVMQSAQVDR
HAAVAHDMVARGTAFRCYVTPEELQARRDLGEEKRQAAKQEGISDAEKEALLAEASQLLAPFRSPYRDGASPPSPDAPFT
VRLRAPESGPRTVEDGVQGTVTIDASEIDDLVMLRADGTPTYMLAVVVDDHDMGITHVIRGDDHLRNTFRQVPIYEAMGW
SVPNFSHVPMIHGNDGAKLSKRHGALSTTAYRDMGYLPEAMKAYLLRLGWSHGDQEIFTDEEAVQVFDVSGINKAPARLD
LDKLATVNAHFMRLAADERLFDLICPVLSKNCSLSDAEVARIRAALPHMKDRGSTLIELANAFAFLYAKRPLELNKNAVK
ALSDEGKLRLKGLYDDLQRMSQWSGASISETIKSYCAATGLSMGQIGPPLRAALTGGLPAPDLAPVMDWLGREETLARID
DQLAG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=503, Percent_Identity=29.4234592445328, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI4826960, Length=123, Percent_Identity=35.7723577235772, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=39.3004115226337, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=33.5849056603774, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=30.1010101010101, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=30.6930693069307, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=155, Percent_Identity=30.3225806451613, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321192, Length=237, Percent_Identity=25.7383966244726, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=515, Percent_Identity=30.0970873786408, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_HYPNA (Q0C1A6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760487.1
- ProteinModelPortal:   Q0C1A6
- SMR:   Q0C1A6
- STRING:   Q0C1A6
- GeneID:   4289642
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1783
- NMPDR:   fig|228405.5.peg.1717
- TIGR:   HNE_1783
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q0C1A6
- ProtClustDB:   PRK01406
- BioCyc:   HNEP81032:HNE_1783-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53324; Mature: 53193

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 262-262

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVTRFAPSPTGMLHIGGARTALFNYLFARRNGGRFLLRIEDTDRERSTQEATDAILDA
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MEWLGLTPDEPPVMQSAQVDRHAAVAHDMVARGTAFRCYVTPEELQARRDLGEEKRQAAK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHH
QEGISDAEKEALLAEASQLLAPFRSPYRDGASPPSPDAPFTVRLRAPESGPRTVEDGVQG
HCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHCCCCE
TVTIDASEIDDLVMLRADGTPTYMLAVVVDDHDMGITHVIRGDDHLRNTFRQVPIYEAMG
EEEEEHHCCCCEEEEEECCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHCCCHHHHC
WSVPNFSHVPMIHGNDGAKLSKRHGALSTTAYRDMGYLPEAMKAYLLRLGWSHGDQEIFT
CCCCCCCCCCEEECCCCCCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
DEEAVQVFDVSGINKAPARLDLDKLATVNAHFMRLAADERLFDLICPVLSKNCSLSDAEV
CCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARIRAALPHMKDRGSTLIELANAFAFLYAKRPLELNKNAVKALSDEGKLRLKGLYDDLQR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHH
MSQWSGASISETIKSYCAATGLSMGQIGPPLRAALTGGLPAPDLAPVMDWLGREETLARI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
DDQLAG
HHHHCC
>Mature Secondary Structure 
SVVTRFAPSPTGMLHIGGARTALFNYLFARRNGGRFLLRIEDTDRERSTQEATDAILDA
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MEWLGLTPDEPPVMQSAQVDRHAAVAHDMVARGTAFRCYVTPEELQARRDLGEEKRQAAK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHH
QEGISDAEKEALLAEASQLLAPFRSPYRDGASPPSPDAPFTVRLRAPESGPRTVEDGVQG
HCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHCCCCE
TVTIDASEIDDLVMLRADGTPTYMLAVVVDDHDMGITHVIRGDDHLRNTFRQVPIYEAMG
EEEEEHHCCCCEEEEEECCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHCCCHHHHC
WSVPNFSHVPMIHGNDGAKLSKRHGALSTTAYRDMGYLPEAMKAYLLRLGWSHGDQEIFT
CCCCCCCCCCEEECCCCCCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
DEEAVQVFDVSGINKAPARLDLDKLATVNAHFMRLAADERLFDLICPVLSKNCSLSDAEV
CCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARIRAALPHMKDRGSTLIELANAFAFLYAKRPLELNKNAVKALSDEGKLRLKGLYDDLQR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHH
MSQWSGASISETIKSYCAATGLSMGQIGPPLRAALTGGLPAPDLAPVMDWLGREETLARI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
DDQLAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA