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The map label for this gene is gltA [H]

Identifier: 114797964

GI number: 114797964

Start: 1826343

End: 1827647

Strand: Reverse

Name: gltA [H]

Synonym: HNE_1782

Alternate gene names: 114797964

Gene position: 1827647-1826343 (Counterclockwise)

Preceding gene: 114798943

Following gene: 114800212

Centisome position: 49.33

GC content: 57.7

Gene sequence:

>1305_bases
ATGGAAAACAAGGTTAAAGACGCTGGTACGGCCAAACTTGAAGTCGGCGGTAAAACAGTCAGTTTGCCGGTCTATTCTGG
CACGCACGGTCCAAACGTAATCGACATCCGTAAGCTTTATGCTGAAGTCGATCATTTCACGCTCGACCCCGGCTTCACGT
CGACAGGTAGCTGCGAGAGCCAGATTACGTTCATCGACGGTGATGAAGGCATTCTGCTGCACCGGGGCTATCCGATTGAG
CAGCTCGCCGAAAAGTCGAGCTATCTGGAGCTCTGCTATCTGCTACTCAATGGCGAGCTGCCCACCAAGGATGAGTACGA
AGCCTTCGGCAAGGACGTGCGTCAGCACACGCTTCTTCACACACAGCAGGATCGCTTCTTCGACGGGTTCCGGCGCGACA
GCCACCCGATGGCGATGCTGACCGGCGCAGTTGGCGCCATGGCGGCCTTTTACCCCGGCGCAATGGGCAATGAAGATCCT
GAGGAGCGTCGCCGCGACTCCATCCGGCTTGTCGCGAAGATGCCGACAATTGCAGCGCGCTGCATGAAGTACAATCTCGG
ACAGAAGTTCGTGGACCCGCAGAACCGCCTGTCCTACTCAGAAAACTTCCTGAACATGTGCTTCTCCGTGAGCGCGGAAG
ACTATGAAGTGAACCCGGTTCTTGCGCGTGCGATGGACCGCTTCTTCATGCTTCATGCGGACCATGAACAGAACGCGTCT
ACGTCCACCGTTCGCCTGGTCGCGTCTTCGGGCGCGCACCCCTTCGCCGCGATTGCGGCCGGTGTGGCAAGCCTCTGGGG
CCCCAGCCATGGCGGCGCCAACGAAGCGGCATTGCGTCAGCTTCGCGAGATCGGCGACATCAGCAACGTTCCGGACTTCG
TGAAGAAGGTAAAGGACAAGTCGAGCGGTGTCCGCTTGATGGGCTTTGGCCACCGGGTTTACAAAAACTACGACCCGCGC
GCGAAAGTCATGCAGAAAACCGCTCACGAGGTGCTCGACGCGCTCGACCTTCGCGACCATCCGCTGCTCAAAGTTGCAAT
GGAACTTGAGCGGATTGCCCTTCAGGATGAGTACTTCGTTGCGAAGAAACTTTACCCCAACGTCGATTTCTATTCGGGTA
TTATTCTCGAAGCGATGGGCTTCCCGACCAAGATGTTCACCGTCCTGTTTGCCGTGGCGCGGACCGTGGGCTGGGTCGCT
CAGATGAACGAGATGCTCGAAGATCCGAGCCAGAAAATCGGCCGTCCTCGCCAGATCTATACCGGCGCCGCAATGCGCGA
CTATACGGCAATGACTAGCCGCTAG

Upstream 100 bases:

>100_bases
GGCTCGGCCGGGAAGAAACGCTCGCAAGAATTGATGACCAACTCGCCGGCTGACACGCAAGCCAAAGCCGGCCAACAAAC
ATGAACAGGGTTAGAGGCTG

Downstream 100 bases:

>100_bases
TTTTTACGGACTTCAGTTCATCAATTATGGCCCCGGCTGCGCGCGTCGCTGTCGGGGCCTCTGTTTTTATGAGCTGTTTC
AAAGCAACGGCTTGGATTGC

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MENKVKDAGTAKLEVGGKTVSLPVYSGTHGPNVIDIRKLYAEVDHFTLDPGFTSTGSCESQITFIDGDEGILLHRGYPIE
QLAEKSSYLELCYLLLNGELPTKDEYEAFGKDVRQHTLLHTQQDRFFDGFRRDSHPMAMLTGAVGAMAAFYPGAMGNEDP
EERRRDSIRLVAKMPTIAARCMKYNLGQKFVDPQNRLSYSENFLNMCFSVSAEDYEVNPVLARAMDRFFMLHADHEQNAS
TSTVRLVASSGAHPFAAIAAGVASLWGPSHGGANEAALRQLREIGDISNVPDFVKKVKDKSSGVRLMGFGHRVYKNYDPR
AKVMQKTAHEVLDALDLRDHPLLKVAMELERIALQDEYFVAKKLYPNVDFYSGIILEAMGFPTKMFTVLFAVARTVGWVA
QMNEMLEDPSQKIGRPRQIYTGAAMRDYTAMTSR

Sequences:

>Translated_434_residues
MENKVKDAGTAKLEVGGKTVSLPVYSGTHGPNVIDIRKLYAEVDHFTLDPGFTSTGSCESQITFIDGDEGILLHRGYPIE
QLAEKSSYLELCYLLLNGELPTKDEYEAFGKDVRQHTLLHTQQDRFFDGFRRDSHPMAMLTGAVGAMAAFYPGAMGNEDP
EERRRDSIRLVAKMPTIAARCMKYNLGQKFVDPQNRLSYSENFLNMCFSVSAEDYEVNPVLARAMDRFFMLHADHEQNAS
TSTVRLVASSGAHPFAAIAAGVASLWGPSHGGANEAALRQLREIGDISNVPDFVKKVKDKSSGVRLMGFGHRVYKNYDPR
AKVMQKTAHEVLDALDLRDHPLLKVAMELERIALQDEYFVAKKLYPNVDFYSGIILEAMGFPTKMFTVLFAVARTVGWVA
QMNEMLEDPSQKIGRPRQIYTGAAMRDYTAMTSR
>Mature_434_residues
MENKVKDAGTAKLEVGGKTVSLPVYSGTHGPNVIDIRKLYAEVDHFTLDPGFTSTGSCESQITFIDGDEGILLHRGYPIE
QLAEKSSYLELCYLLLNGELPTKDEYEAFGKDVRQHTLLHTQQDRFFDGFRRDSHPMAMLTGAVGAMAAFYPGAMGNEDP
EERRRDSIRLVAKMPTIAARCMKYNLGQKFVDPQNRLSYSENFLNMCFSVSAEDYEVNPVLARAMDRFFMLHADHEQNAS
TSTVRLVASSGAHPFAAIAAGVASLWGPSHGGANEAALRQLREIGDISNVPDFVKKVKDKSSGVRLMGFGHRVYKNYDPR
AKVMQKTAHEVLDALDLRDHPLLKVAMELERIALQDEYFVAKKLYPNVDFYSGIILEAMGFPTKMFTVLFAVARTVGWVA
QMNEMLEDPSQKIGRPRQIYTGAAMRDYTAMTSR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=398, Percent_Identity=29.6482412060301, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=62.5882352941176, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=31.5508021390374, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=384, Percent_Identity=30.7291666666667, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319850, Length=334, Percent_Identity=30.5389221556886, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324328, Length=367, Percent_Identity=28.0653950953678, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325257, Length=366, Percent_Identity=25.1366120218579, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640126, Length=379, Percent_Identity=27.7044854881267, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640124, Length=379, Percent_Identity=27.7044854881267, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356863, Length=336, Percent_Identity=28.8690476190476, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48461; Mature: 48461

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKVKDAGTAKLEVGGKTVSLPVYSGTHGPNVIDIRKLYAEVDHFTLDPGFTSTGSCES
CCCCCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCCC
QITFIDGDEGILLHRGYPIEQLAEKSSYLELCYLLLNGELPTKDEYEAFGKDVRQHTLLH
EEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TQQDRFFDGFRRDSHPMAMLTGAVGAMAAFYPGAMGNEDPEERRRDSIRLVAKMPTIAAR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CMKYNLGQKFVDPQNRLSYSENFLNMCFSVSAEDYEVNPVLARAMDRFFMLHADHEQNAS
HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
TSTVRLVASSGAHPFAAIAAGVASLWGPSHGGANEAALRQLREIGDISNVPDFVKKVKDK
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC
SSGVRLMGFGHRVYKNYDPRAKVMQKTAHEVLDALDLRDHPLLKVAMELERIALQDEYFV
CCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
AKKLYPNVDFYSGIILEAMGFPTKMFTVLFAVARTVGWVAQMNEMLEDPSQKIGRPRQIY
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEE
TGAAMRDYTAMTSR
CCCHHHHHHHHCCC
>Mature Secondary Structure
MENKVKDAGTAKLEVGGKTVSLPVYSGTHGPNVIDIRKLYAEVDHFTLDPGFTSTGSCES
CCCCCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCCC
QITFIDGDEGILLHRGYPIEQLAEKSSYLELCYLLLNGELPTKDEYEAFGKDVRQHTLLH
EEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TQQDRFFDGFRRDSHPMAMLTGAVGAMAAFYPGAMGNEDPEERRRDSIRLVAKMPTIAAR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CMKYNLGQKFVDPQNRLSYSENFLNMCFSVSAEDYEVNPVLARAMDRFFMLHADHEQNAS
HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
TSTVRLVASSGAHPFAAIAAGVASLWGPSHGGANEAALRQLREIGDISNVPDFVKKVKDK
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC
SSGVRLMGFGHRVYKNYDPRAKVMQKTAHEVLDALDLRDHPLLKVAMELERIALQDEYFV
CCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
AKKLYPNVDFYSGIILEAMGFPTKMFTVLFAVARTVGWVAQMNEMLEDPSQKIGRPRQIY
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEE
TGAAMRDYTAMTSR
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]