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The map label for this gene is sbnA [H]

Identifier: 114798912

GI number: 114798912

Start: 1435952

End: 1436968

Strand: Reverse

Name: sbnA [H]

Synonym: HNE_1409

Alternate gene names: 114798912

Gene position: 1436968-1435952 (Counterclockwise)

Preceding gene: 114797337

Following gene: 114798039

Centisome position: 38.78

GC content: 65.19

Gene sequence:

>1017_bases
ATGACAGCCAGAACAGACATGACGGATTGCCCTTTTGGAATCGGCAATACCCCGCTGACCCCCTTCTCCATCACCCTGAA
CGGCATTCGCCATGAATTGTTGCTGAAGGAAGAACGGTGCAACGCGTTCGGCTCGGTCAAGGACCGGGTGGCGTGGTACA
TCCTCTCGCAGACCATTGCTGAAAAGGGCCCTGTCGAGGCTGTCGTGGATGCGTCCTCGGGCAATTACGGCTACGCGCTG
GCCAGCATCTGCCAGGAGATGGGCATCGCGGCGACGATTGTGTCGTCATCCTCGATCAGCGCGTTCAATGCCGAGGGCAT
CAAGCGGGCGGGCGCGCGGCTGGTGATCGCCGAGCCGGCGCCGGGAGAATGCTCCAACGCGGCGCGGATGCGGGTGGCGG
GCGAGATCAGCGCCGCCGAGGGACAGACCTTCCTCAACCAGTACGCCAATTTCATGAACCCGGCCTCCCACGAGCACTGG
ACGGCGCCGGAAGTGTTTGCAGACGGGCCGTTTGATGCCTGCTTCGTCACATCTTCCTCCGGCGGCACGGCGCGGGGCTT
TTCGGACTATCTGAAGGCCGAGCGGAACGGCACGCAGCTGGTTCTGGTGGAGCCGGAAGCATCCTCCGCCTTCCTTGCGC
CCGCTTGCGAGAGCGGCAAGCTGAAAGTGCCGGGCTATGGCAGCCAGCGGCGATCCAGCTTTGCCGGGATGCGACCAGAC
CCCGGCATGCTGCGGGTGGATGAGGCGTCGGTTCTGGCGGCCTTCACCCTGTTGGAAGCGCGCGGGCTGACGCATATCGG
GCTGTCCAGCGTGGGCGTGGTGGTCGGCGCGCTGGAATGGCTGTCGCGGCAGAGTGAGCGCAGGCGGGTGGTGTGCGTCT
GCGCCGATGGGGACGAACGGTATCTGGACGAGATCGACAGCCGGTATATCCCGTCGGTTGGCGCGGACGCGTTTGATGCG
GCGCGCGCGCGGCTGGAGCCGGTGATCGCGTCTATGGCGCCTGAGGTTGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCATTACGTATTCATACAAAGCAGATGCCGCATTCCCGAACGCGCCGGAGACTGCTAGCCATGTGCTGCGCCGCAACAG
ACCAAGATCGAGGCAGGCTG

Downstream 100 bases:

>100_bases
GCCTTTGCGGCGCCGGCCAAGCCCGGCCGGGCCGGCGAACACCGCGTTGCCCCAATTCTCCCTTAAAAGACCGTAATGTG
AGCAAGCGCTCGCCGAAGTC

Product: pyridoxal-phosphate-dependent enzyme

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTARTDMTDCPFGIGNTPLTPFSITLNGIRHELLLKEERCNAFGSVKDRVAWYILSQTIAEKGPVEAVVDASSGNYGYAL
ASICQEMGIAATIVSSSSISAFNAEGIKRAGARLVIAEPAPGECSNAARMRVAGEISAAEGQTFLNQYANFMNPASHEHW
TAPEVFADGPFDACFVTSSSGGTARGFSDYLKAERNGTQLVLVEPEASSAFLAPACESGKLKVPGYGSQRRSSFAGMRPD
PGMLRVDEASVLAAFTLLEARGLTHIGLSSVGVVVGALEWLSRQSERRRVVCVCADGDERYLDEIDSRYIPSVGADAFDA
ARARLEPVIASMAPEVAA

Sequences:

>Translated_338_residues
MTARTDMTDCPFGIGNTPLTPFSITLNGIRHELLLKEERCNAFGSVKDRVAWYILSQTIAEKGPVEAVVDASSGNYGYAL
ASICQEMGIAATIVSSSSISAFNAEGIKRAGARLVIAEPAPGECSNAARMRVAGEISAAEGQTFLNQYANFMNPASHEHW
TAPEVFADGPFDACFVTSSSGGTARGFSDYLKAERNGTQLVLVEPEASSAFLAPACESGKLKVPGYGSQRRSSFAGMRPD
PGMLRVDEASVLAAFTLLEARGLTHIGLSSVGVVVGALEWLSRQSERRRVVCVCADGDERYLDEIDSRYIPSVGADAFDA
ARARLEPVIASMAPEVAA
>Mature_337_residues
TARTDMTDCPFGIGNTPLTPFSITLNGIRHELLLKEERCNAFGSVKDRVAWYILSQTIAEKGPVEAVVDASSGNYGYALA
SICQEMGIAATIVSSSSISAFNAEGIKRAGARLVIAEPAPGECSNAARMRVAGEISAAEGQTFLNQYANFMNPASHEHWT
APEVFADGPFDACFVTSSSGGTARGFSDYLKAERNGTQLVLVEPEASSAFLAPACESGKLKVPGYGSQRRSSFAGMRPDP
GMLRVDEASVLAAFTLLEARGLTHIGLSSVGVVVGALEWLSRQSERRRVVCVCADGDERYLDEIDSRYIPSVGADAFDAA
RARLEPVIASMAPEVAA

Specific function: Probable pyridoxal phosphate-dependent enzyme that may be involved in siderophore biosynthesis [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=207, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI295821200, Length=207, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557415, Length=207, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=30.5993690851735, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367138, Length=294, Percent_Identity=27.891156462585, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32566674, Length=304, Percent_Identity=28.6184210526316, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17562970, Length=304, Percent_Identity=28.2894736842105, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=29.2763157894737, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535051, Length=307, Percent_Identity=27.0358306188925, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561720, Length=205, Percent_Identity=30.7317073170732, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=206, Percent_Identity=29.6116504854369, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24643623, Length=221, Percent_Identity=29.8642533936652, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129101, Length=221, Percent_Identity=29.8642533936652, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 35873; Mature: 35742

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARTDMTDCPFGIGNTPLTPFSITLNGIRHELLLKEERCNAFGSVKDRVAWYILSQTIA
CCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHH
EKGPVEAVVDASSGNYGYALASICQEMGIAATIVSSSSISAFNAEGIKRAGARLVIAEPA
CCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHCHHHHHHCCCEEEEECCC
PGECSNAARMRVAGEISAAEGQTFLNQYANFMNPASHEHWTAPEVFADGPFDACFVTSSS
CCCCCCCHHEEEECCCCHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCC
GGTARGFSDYLKAERNGTQLVLVEPEASSAFLAPACESGKLKVPGYGSQRRSSFAGMRPD
CCCCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCEECCCCCCHHHHHCCCCCCC
PGMLRVDEASVLAAFTLLEARGLTHIGLSSVGVVVGALEWLSRQSERRRVVCVCADGDER
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
YLDEIDSRYIPSVGADAFDAARARLEPVIASMAPEVAA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TARTDMTDCPFGIGNTPLTPFSITLNGIRHELLLKEERCNAFGSVKDRVAWYILSQTIA
CCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHH
EKGPVEAVVDASSGNYGYALASICQEMGIAATIVSSSSISAFNAEGIKRAGARLVIAEPA
CCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHCHHHHHHCCCEEEEECCC
PGECSNAARMRVAGEISAAEGQTFLNQYANFMNPASHEHWTAPEVFADGPFDACFVTSSS
CCCCCCCHHEEEECCCCHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCC
GGTARGFSDYLKAERNGTQLVLVEPEASSAFLAPACESGKLKVPGYGSQRRSSFAGMRPD
CCCCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCEECCCCCCHHHHHCCCCCCC
PGMLRVDEASVLAAFTLLEARGLTHIGLSSVGVVVGALEWLSRQSERRRVVCVCADGDER
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
YLDEIDSRYIPSVGADAFDAARARLEPVIASMAPEVAA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 3.6 {S2-}} 0.1 {S2-}} 0.12 {acetyl-CoA}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]