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The map label for this gene is rpoE [H]

Identifier: 114798823

GI number: 114798823

Start: 2444720

End: 2445325

Strand: Reverse

Name: rpoE [H]

Synonym: HNE_2340

Alternate gene names: 114798823

Gene position: 2445325-2444720 (Counterclockwise)

Preceding gene: 114798824

Following gene: 114797856

Centisome position: 66.0

GC content: 59.74

Gene sequence:

>606_bases
ATGACCGCAACTCAGGCACTTTCCCTGTCTGACTTCAAAGCCGCTCTTATGGAGGTAACCCCTCATCTGCGCGCGTTCGC
CCGCAGCCTTTGCGGCAACCCTACCCAAGCCGATGACCTGGTGCAGGACGCGCTGCTCAAAGCGTGGAATGCGCGCGACA
GCTATACAGAGAATACGAGCTTCAAGGCATGGACGTTCACCATCCTTCGCAACTCGTTCTATTCGGAAAAGCGGCGCAGC
TGGCGGTCCACCGGTCTTGAGCCGGGCGTTGCAGAATCGACGCTTGTCGCCTCCGATGACCCCGACAAGGGGCTTGAACT
TGTAGCGCTGCGCAATGCGCTGGAGCATCTTCCCCAGGATCAGCGCGAAGCGATCATCCTGATCGGCGCTGGCGGCATGG
CGTATGAAGAAGCGGCTGAAATTGTCGGCTGCGCTGTCGGAACCATCAAAAGCCGTGTCAGCCGGGCGCGCAAGGCGCTG
GCTGAACTGCTTAACAGCGAACATGGTGTGCCTTCCGGGGCAAGCGGCTCGAATGTCACGTCAGGCGAAGCAGCGTTCAA
CGACATCATGGGCCAGGTTGACGTCCTCAGCCGCCATAACGGATGA

Upstream 100 bases:

>100_bases
GCGTCGTTGGGGAGTCTGTTCCTGACGACTTTTTCAAGCTGCTTGAACAGGCGGAAGCCAAAGCGAAGGCAGGCGTCAAA
TCGCGCCCCGACGGTGAAGC

Downstream 100 bases:

>100_bases
CGGTAGTCAGCGGGGAAGGACGCGCCAAGCGCAGGCGCCTGAGCCTCAGGCTTCAGGTAATTTCTGCCATGGCGGTCGTC
CTTAGCCCGCTGCTGATAAT

Product: ECF subfamily RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MTATQALSLSDFKAALMEVTPHLRAFARSLCGNPTQADDLVQDALLKAWNARDSYTENTSFKAWTFTILRNSFYSEKRRS
WRSTGLEPGVAESTLVASDDPDKGLELVALRNALEHLPQDQREAIILIGAGGMAYEEAAEIVGCAVGTIKSRVSRARKAL
AELLNSEHGVPSGASGSNVTSGEAAFNDIMGQVDVLSRHNG

Sequences:

>Translated_201_residues
MTATQALSLSDFKAALMEVTPHLRAFARSLCGNPTQADDLVQDALLKAWNARDSYTENTSFKAWTFTILRNSFYSEKRRS
WRSTGLEPGVAESTLVASDDPDKGLELVALRNALEHLPQDQREAIILIGAGGMAYEEAAEIVGCAVGTIKSRVSRARKAL
AELLNSEHGVPSGASGSNVTSGEAAFNDIMGQVDVLSRHNG
>Mature_200_residues
TATQALSLSDFKAALMEVTPHLRAFARSLCGNPTQADDLVQDALLKAWNARDSYTENTSFKAWTFTILRNSFYSEKRRSW
RSTGLEPGVAESTLVASDDPDKGLELVALRNALEHLPQDQREAIILIGAGGMAYEEAAEIVGCAVGTIKSRVSRARKALA
ELLNSEHGVPSGASGSNVTSGEAAFNDIMGQVDVLSRHNG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21609; Mature: 21478

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATQALSLSDFKAALMEVTPHLRAFARSLCGNPTQADDLVQDALLKAWNARDSYTENTS
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FKAWTFTILRNSFYSEKRRSWRSTGLEPGVAESTLVASDDPDKGLELVALRNALEHLPQD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCC
QREAIILIGAGGMAYEEAAEIVGCAVGTIKSRVSRARKALAELLNSEHGVPSGASGSNVT
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SGEAAFNDIMGQVDVLSRHNG
CHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TATQALSLSDFKAALMEVTPHLRAFARSLCGNPTQADDLVQDALLKAWNARDSYTENTS
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FKAWTFTILRNSFYSEKRRSWRSTGLEPGVAESTLVASDDPDKGLELVALRNALEHLPQD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCC
QREAIILIGAGGMAYEEAAEIVGCAVGTIKSRVSRARKALAELLNSEHGVPSGASGSNVT
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SGEAAFNDIMGQVDVLSRHNG
CHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]