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The map label for this gene is ribE [H]

Identifier: 114798806

GI number: 114798806

Start: 2131649

End: 2132269

Strand: Reverse

Name: ribE [H]

Synonym: HNE_2057

Alternate gene names: 114798806

Gene position: 2132269-2131649 (Counterclockwise)

Preceding gene: 114798296

Following gene: 114799819

Centisome position: 57.55

GC content: 63.61

Gene sequence:

>621_bases
ATGTTCACAGGTCTCGTCACCGATGTCGGCACTGTCCGCAAGGCAGAGCACCGCAACGGCCTCACCCGGTTTGAGGTCGA
AAGCGGCTACCCGCTCGCCCAGATCGCCATGGGCGCGTCCATTCTCCATTCCGGTGTCTGTCTCACCGTCGTAGACAAAG
GCGAAGCCGAGCGAGGCGCCTGGTTTGCGGTCGAAGCTGTCCCCGAAACCCTGTCGAAAACCATCCTCGGCGGCTGGCAG
GAAGGCACACGCGTCAACCTCGAGCAGAGCCTCAAGCTCGGCGATGAACTCGGCGGACACTTCGTGTTCGGCCATGTCGA
CGGCGTCGGCGAGATTGTCTCGGTCGAGGCCGAAGGCCAGTCCTTCCGCGTCACCATCCGCCCGCCCGCCGCCATCTCGC
GCTACTTCGCCACCAAGGGCTCGGCCGCCGTTGATGGCGTCTCGCTCACGGTCGCCGCCGCGCTGGAGAATGGAGACTTC
CAGGTCGCCATCATCCCGCATACCTGGGAAGTCACCACGCTTGCCGCGTTGAAGCCCGGCACAAAGGTAAACCTCGAAAT
TGATATGCTTGCGCGCTATGTGGCGCGGATGATCGGTGCGGACGCGCCACAAGGGAGTTAG

Upstream 100 bases:

>100_bases
ATCTGCGGCACGCTGGCGCCCTGTCGCGACGGAACGGATTGGGGATGACGTCCTCGATACTTATGTTCGATCTGAGCAAT
AGGCACCCAGGATAAGAACC

Downstream 100 bases:

>100_bases
ATAATGAGCAGCGAGTTCACCGCCGCCATCTCCTCGATTGAGGAAATAATCGAAGACGCCCGCAACGGGCGCATGTTCAT
CCTGGTGGATGCCGAAGACC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MFTGLVTDVGTVRKAEHRNGLTRFEVESGYPLAQIAMGASILHSGVCLTVVDKGEAERGAWFAVEAVPETLSKTILGGWQ
EGTRVNLEQSLKLGDELGGHFVFGHVDGVGEIVSVEAEGQSFRVTIRPPAAISRYFATKGSAAVDGVSLTVAAALENGDF
QVAIIPHTWEVTTLAALKPGTKVNLEIDMLARYVARMIGADAPQGS

Sequences:

>Translated_206_residues
MFTGLVTDVGTVRKAEHRNGLTRFEVESGYPLAQIAMGASILHSGVCLTVVDKGEAERGAWFAVEAVPETLSKTILGGWQ
EGTRVNLEQSLKLGDELGGHFVFGHVDGVGEIVSVEAEGQSFRVTIRPPAAISRYFATKGSAAVDGVSLTVAAALENGDF
QVAIIPHTWEVTTLAALKPGTKVNLEIDMLARYVARMIGADAPQGS
>Mature_206_residues
MFTGLVTDVGTVRKAEHRNGLTRFEVESGYPLAQIAMGASILHSGVCLTVVDKGEAERGAWFAVEAVPETLSKTILGGWQ
EGTRVNLEQSLKLGDELGGHFVFGHVDGVGEIVSVEAEGQSFRVTIRPPAAISRYFATKGSAAVDGVSLTVAAALENGDF
QVAIIPHTWEVTTLAALKPGTKVNLEIDMLARYVARMIGADAPQGS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=190, Percent_Identity=32.6315789473684, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319733, Length=210, Percent_Identity=32.8571428571429, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 21793; Mature: 21793

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLVTDVGTVRKAEHRNGLTRFEVESGYPLAQIAMGASILHSGVCLTVVDKGEAERGA
CCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
WFAVEAVPETLSKTILGGWQEGTRVNLEQSLKLGDELGGHFVFGHVDGVGEIVSVEAEGQ
EEEEEHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
SFRVTIRPPAAISRYFATKGSAAVDGVSLTVAAALENGDFQVAIIPHTWEVTTLAALKPG
EEEEEEECCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCEEEEEEECCC
TKVNLEIDMLARYVARMIGADAPQGS
CEEEEEHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFTGLVTDVGTVRKAEHRNGLTRFEVESGYPLAQIAMGASILHSGVCLTVVDKGEAERGA
CCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
WFAVEAVPETLSKTILGGWQEGTRVNLEQSLKLGDELGGHFVFGHVDGVGEIVSVEAEGQ
EEEEEHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
SFRVTIRPPAAISRYFATKGSAAVDGVSLTVAAALENGDFQVAIIPHTWEVTTLAALKPG
EEEEEEECCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCEEEEEEECCC
TKVNLEIDMLARYVARMIGADAPQGS
CEEEEEHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]