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The map label for this gene is rlmE

Identifier: 114798748

GI number: 114798748

Start: 1848098

End: 1848802

Strand: Direct

Name: rlmE

Synonym: HNE_1802

Alternate gene names: 114798748

Gene position: 1848098-1848802 (Clockwise)

Preceding gene: 114799621

Following gene: 114797683

Centisome position: 49.88

GC content: 60.85

Gene sequence:

>705_bases
ATGAGTGACGACGACAGAAGGCGGTGGAAAGGACCGGGGCCGGAACGCCAGGATTCCGGACGGCGGTCGACCGAACGCAA
GGTCATTGCCCGCAACGCCCGGACTGAGAGCTCGAAACGCTGGATTGAGCGCCAGCTTCAGGACCCCTACGTCCGTAAAG
CCAAGGATGAAGGCTATCGCTCGAGAGCTGCCTACAAACTGCTCGAGATCGACGCCGCTGCGAAAATCCTGCGTAAGGGC
ATGCGCGTGGTCGATCTTGGTTGCGCGCCGGGCGGATGGATACAGGTCTCGCTCCAGCAGGGCGCTGCAGAAGTGGTCGG
CATTGACCTTCTGCCGCTGGACCCGATCGAAGGCGCCACGATCATCGAAGGCGACGTCAACAACCCAGACGATGTGGCAA
GAATGATGGCTGGGCTCTCGGGCACACCCGACCTCATCCTGTCTGACATGGCCGCCAACACGACCGGCCACAAACAGACC
GACCACCTCCGCACTGTTGCCCTGGTCGAGATGGCTGTCGCCTTCGCAATCGAACACCTCGATGATGGCGGCGCTTTCTG
CGCCAAGGTCTTCCAGGGCGGCGCCACCAAAGATGTTCTCAATCTGCTGAAACAGCATTTCAGGACGGTAAAACACATCA
AACCCGCCGCTAGCCGCGCGGGAAGCCCGGAAATCTATGTGGTCGCCAAGGGTTTCCGGCGTTAG

Upstream 100 bases:

>100_bases
CGTGAAGGCCTTCTATTGTCCATGATGTATGGTCAGCCCAAGAACCGGAGCCGGCGGCGCAAAGGGCGCGGCGGTCGCCC
CAGCCCTCAGGAGGCTCCAA

Downstream 100 bases:

>100_bases
TCTGACCGGAAACGGGAGGACAAAATGGCACTTACGGGCGGCTGTTACTGCGGAAACATCAGGTATGAAGCGGGCGGTGA
TCCCGTAATGCGGGCGCGTT

Product: putative ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSDDDRRRWKGPGPERQDSGRRSTERKVIARNARTESSKRWIERQLQDPYVRKAKDEGYRSRAAYKLLEIDAAAKILRKG
MRVVDLGCAPGGWIQVSLQQGAAEVVGIDLLPLDPIEGATIIEGDVNNPDDVARMMAGLSGTPDLILSDMAANTTGHKQT
DHLRTVALVEMAVAFAIEHLDDGGAFCAKVFQGGATKDVLNLLKQHFRTVKHIKPAASRAGSPEIYVVAKGFRR

Sequences:

>Translated_234_residues
MSDDDRRRWKGPGPERQDSGRRSTERKVIARNARTESSKRWIERQLQDPYVRKAKDEGYRSRAAYKLLEIDAAAKILRKG
MRVVDLGCAPGGWIQVSLQQGAAEVVGIDLLPLDPIEGATIIEGDVNNPDDVARMMAGLSGTPDLILSDMAANTTGHKQT
DHLRTVALVEMAVAFAIEHLDDGGAFCAKVFQGGATKDVLNLLKQHFRTVKHIKPAASRAGSPEIYVVAKGFRR
>Mature_233_residues
SDDDRRRWKGPGPERQDSGRRSTERKVIARNARTESSKRWIERQLQDPYVRKAKDEGYRSRAAYKLLEIDAAAKILRKGM
RVVDLGCAPGGWIQVSLQQGAAEVVGIDLLPLDPIEGATIIEGDVNNPDDVARMMAGLSGTPDLILSDMAANTTGHKQTD
HLRTVALVEMAVAFAIEHLDDGGAFCAKVFQGGATKDVLNLLKQHFRTVKHIKPAASRAGSPEIYVVAKGFRR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=211, Percent_Identity=39.8104265402844, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI194097365, Length=189, Percent_Identity=33.8624338624339, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI29029591, Length=204, Percent_Identity=34.3137254901961, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI29029589, Length=204, Percent_Identity=34.3137254901961, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI7110661, Length=204, Percent_Identity=34.3137254901961, Blast_Score=114, Evalue=6e-26,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=41.6666666666667, Blast_Score=148, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=204, Percent_Identity=36.7647058823529, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71987550, Length=196, Percent_Identity=34.6938775510204, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=34.6938775510204, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=34.6938775510204, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144487, Length=112, Percent_Identity=34.8214285714286, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=31.0526315789474, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=97, Percent_Identity=40.2061855670103, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=35.8490566037736, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI18859957, Length=189, Percent_Identity=34.3915343915344, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648639, Length=197, Percent_Identity=30.4568527918782, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_HYPNA (Q0C187)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760506.1
- ProteinModelPortal:   Q0C187
- SMR:   Q0C187
- STRING:   Q0C187
- GeneID:   4287983
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1802
- NMPDR:   fig|228405.5.peg.1734
- TIGR:   HNE_1802
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q0C187
- ProtClustDB:   CLSK2531882
- BioCyc:   HNEP81032:HNE_1802-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25714; Mature: 25582

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 91-91 BINDING 93-93 BINDING 109-109 BINDING 125-125 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDDRRRWKGPGPERQDSGRRSTERKVIARNARTESSKRWIERQLQDPYVRKAKDEGYR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCHH
SRAAYKLLEIDAAAKILRKGMRVVDLGCAPGGWIQVSLQQGAAEVVGIDLLPLDPIEGAT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCE
IIEGDVNNPDDVARMMAGLSGTPDLILSDMAANTTGHKQTDHLRTVALVEMAVAFAIEHL
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DDGGAFCAKVFQGGATKDVLNLLKQHFRTVKHIKPAASRAGSPEIYVVAKGFRR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SDDDRRRWKGPGPERQDSGRRSTERKVIARNARTESSKRWIERQLQDPYVRKAKDEGYR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCHH
SRAAYKLLEIDAAAKILRKGMRVVDLGCAPGGWIQVSLQQGAAEVVGIDLLPLDPIEGAT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCE
IIEGDVNNPDDVARMMAGLSGTPDLILSDMAANTTGHKQTDHLRTVALVEMAVAFAIEHL
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DDGGAFCAKVFQGGATKDVLNLLKQHFRTVKHIKPAASRAGSPEIYVVAKGFRR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA