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The map label for this gene is gidB

Identifier: 114798745

GI number: 114798745

Start: 3697029

End: 3697652

Strand: Reverse

Name: gidB

Synonym: HNE_3562

Alternate gene names: 114798745

Gene position: 3697652-3697029 (Counterclockwise)

Preceding gene: 114797761

Following gene: 114798917

Centisome position: 99.8

GC content: 58.01

Gene sequence:

>624_bases
ATGAGTGAGCGAGACGACCGCGCGGCCTTTCTGGCCGCCAATGATGTTTCACGTGAAACATTGGACCGGCTGGATCGGGT
TATCGACACGCTCGATGTCTGGCGGCAGAAAAGCAATCTGATCGGGCCGAAGGAATGGCCGCAGATATGGACACGCCATG
TCGGGGACTCCTGGCAGCTTCTCGATCATATTCCGGAAACGGCCCACCTGGTCGATCTTGGATCGGGCGCGGGCTTTCCG
GGATTGATCATTGCGGCGGCACGCTCGTTGGGCCACGTGACGATGATCGAAAGCGTGGGAAAGAAGTGCGCCTTTTTACG
GGCTGCGATTCAAGAAGCGGACCTCAGTGCGGCGGTTCACCAGGGCCGGGTCGAAGCCGCGCCTCCGATAAAGGCAGAAT
TCGTTACCGCGCGCGCTTTTGCGCCGCTGCCGGAACTCCTTGATTACGCTGCACCTTGGCTCCGAAAAGGCGCGGTCGGC
GTATTTCCAAAGGGCGAACGCTGGAATGAGGAATTGACTGCGGCTAGGCAAAGATGGAATTTCGCTTATGAAGCGATTCC
AAGTCGCAGTGGTGGGTCAGGCGTCATCTTGATTATCAGGGAGGTCGCACGTCGTAATGACTAG

Upstream 100 bases:

>100_bases
AGGCACGCTGGGTCAGGCCGGGCGGATCGAAGGCGTTACACCGGGCGCACTGACGGCGCTACTCGGTCATGTCCGCCGCG
TCCGGAAAGCTGGGTGACAT

Downstream 100 bases:

>100_bases
ACCCAGAATATTTGCCGTGGTGAATCAAAAGGGCGGGGTCGGAAAGACCACGACCTCGATCAATCTCGGAACAGCTCTTG
CTGCCGTTGGCCGCCGCGTC

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSERDDRAAFLAANDVSRETLDRLDRVIDTLDVWRQKSNLIGPKEWPQIWTRHVGDSWQLLDHIPETAHLVDLGSGAGFP
GLIIAAARSLGHVTMIESVGKKCAFLRAAIQEADLSAAVHQGRVEAAPPIKAEFVTARAFAPLPELLDYAAPWLRKGAVG
VFPKGERWNEELTAARQRWNFAYEAIPSRSGGSGVILIIREVARRND

Sequences:

>Translated_207_residues
MSERDDRAAFLAANDVSRETLDRLDRVIDTLDVWRQKSNLIGPKEWPQIWTRHVGDSWQLLDHIPETAHLVDLGSGAGFP
GLIIAAARSLGHVTMIESVGKKCAFLRAAIQEADLSAAVHQGRVEAAPPIKAEFVTARAFAPLPELLDYAAPWLRKGAVG
VFPKGERWNEELTAARQRWNFAYEAIPSRSGGSGVILIIREVARRND
>Mature_206_residues
SERDDRAAFLAANDVSRETLDRLDRVIDTLDVWRQKSNLIGPKEWPQIWTRHVGDSWQLLDHIPETAHLVDLGSGAGFPG
LIIAAARSLGHVTMIESVGKKCAFLRAAIQEADLSAAVHQGRVEAAPPIKAEFVTARAFAPLPELLDYAAPWLRKGAVGV
FPKGERWNEELTAARQRWNFAYEAIPSRSGGSGVILIIREVARRND

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_HYPNA (Q0BWB0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762233.1
- ProteinModelPortal:   Q0BWB0
- SMR:   Q0BWB0
- STRING:   Q0BWB0
- GeneID:   4287980
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3562
- NMPDR:   fig|228405.5.peg.3411
- TIGR:   HNE_3562
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   MFHVEQS
- BioCyc:   HNEP81032:HNE_3562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22897; Mature: 22766

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERDDRAAFLAANDVSRETLDRLDRVIDTLDVWRQKSNLIGPKEWPQIWTRHVGDSWQL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
LDHIPETAHLVDLGSGAGFPGLIIAAARSLGHVTMIESVGKKCAFLRAAIQEADLSAAVH
HHHCCCHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRVEAAPPIKAEFVTARAFAPLPELLDYAAPWLRKGAVGVFPKGERWNEELTAARQRWN
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FAYEAIPSRSGGSGVILIIREVARRND
HHHHHCCCCCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure 
SERDDRAAFLAANDVSRETLDRLDRVIDTLDVWRQKSNLIGPKEWPQIWTRHVGDSWQL
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
LDHIPETAHLVDLGSGAGFPGLIIAAARSLGHVTMIESVGKKCAFLRAAIQEADLSAAVH
HHHCCCHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRVEAAPPIKAEFVTARAFAPLPELLDYAAPWLRKGAVGVFPKGERWNEELTAARQRWN
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FAYEAIPSRSGGSGVILIIREVARRND
HHHHHCCCCCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA