Click here to switch to the map view.

The map label for this gene is rimO

Identifier: 114798740

GI number: 114798740

Start: 2020928

End: 2022283

Strand: Direct

Name: rimO

Synonym: HNE_1951

Alternate gene names: 114798740

Gene position: 2020928-2022283 (Clockwise)

Preceding gene: 114797359

Following gene: 114798987

Centisome position: 54.55

GC content: 62.83

Gene sequence:

>1356_bases
ATGACCCTCAATCTCGACCGCCCGGCGCCTCTGCGCCCCAAAACCCAGCCGAAAGTCGGCATCGTTTCCCTTGGCTGCCC
CAAGGCGCTGGTCGATTCCGAACGCATCATCACGCGCCTGCGCGCCGAGGGCTATCAGATCGCGCCCGACTATGCGGGAG
CGGACCTTGTTCTGGTCAACACCTGCGGCTTCCTCGACTCCGCCCGCGATGAAAGCCTCGCGGCCATCGGCGAGGCAATC
GAAGCGAACGGCAAAGTCATCGTCACCGGCTGCCTCGGCGCTGAGCCGGAGGTGATCCAGCGGGCCCACCCCAAGGTACT
CGCCGTCACGGGCCCCCATCAGTACGAAGCGGTGATGGACGCCGTCCACACCCACCTCACCCCGCCGCACAATCCGCACA
TGGACCTGGTGCCCGAGAGCGGCCTGCGCCTGACACCCCGCCATTATGCGTATTTGAAAATCTCCGAAGGCTGCAACAAT
CGCTGCTCCTTCTGCATCATCCCGAAACTGCGCGGCGATCTCGCCTCACGCCCCATTCATCACGTGCTGATGGAAGCGGA
AAAACTGGTCGCCGCCGGCGTGAAGGAACTTCTCGTCATCAGCCAGGATACCTCCGCCTACGGACTGGACATTCGCTACA
AGCCGGAAACCTGGCGCGGCAGCGAATACGAAACCCGTTTCCTCGATCTTGCCAAAGGCCTCGGCAGCCTCGGCGTCTGG
ACACGGATGCACTACGTCTACCCCTATCCGCATGTGGACAATGTCATCCCCCTGATGGCCGAAGGCCTGATCACGCCCTA
TCTCGACATCCCGTTCCAGCACGCCTCCGCGCCCGTCCTCAAAGCCATGAAACGCCCTGGAAATCAGGATAAAGTGCTCG
GCCGCATCGCCAAATGGCGCGAGCAGGTGCCAGACCTCGCCATCCGCTCAACCTTCATCGTCGGCTTCCCCGGTGAAACG
GAAGAAGACTTCCAGATCCTGCTGGACTTCATCCGTGAAGCGAAGATCGACCGCGCCGGCTGCTTCAAATACGAGAACGT
CGCCCATGCCGAAAGCGGCAAGCTGGAGGGTCATGTTCCCGAAGAAGTCAAAGAAGAACGCTGGCACCGCTTCATGACGG
CGCAGGCAGAAGTCTCTGCCGCCCGCGCGGAAGCCCAGGTTGGCCGCACGCTGGACGTCATCATCGACGGCCCCGGCGAA
GAGGCCGGCGAGATGATCGCCCGCACGCAGGCCGATGCGCCCGAAGTCGACGCGGTGGTCTATCTGGAAAACGCCGCCCA
CCTGAAGGCAGGCGACATCATCCGGGCAGAAATCACCGGCGCCGACGATTACGACCTCTACGCAACGCCCGCCTGA

Upstream 100 bases:

>100_bases
CCTCAGACGGCTCGGTAACAAATCCTGATACCCACTCCTCATTGCAAAGCCGTGCGGGTAGCGCTTTTCTAAGCGCCTGT
TTCAGGATAGCAGGGCGCCC

Downstream 100 bases:

>100_bases
TTATGGGCGAGCCCGCATCCGGGCGCCCGCCTCCTGCGCTTCGGCAAGCCTCTGCCCCATCGCCTTGTCCATATTGGCGG
CAACGCGTGAAAGTGTGTCA

Product: 30S ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTLNLDRPAPLRPKTQPKVGIVSLGCPKALVDSERIITRLRAEGYQIAPDYAGADLVLVNTCGFLDSARDESLAAIGEAI
EANGKVIVTGCLGAEPEVIQRAHPKVLAVTGPHQYEAVMDAVHTHLTPPHNPHMDLVPESGLRLTPRHYAYLKISEGCNN
RCSFCIIPKLRGDLASRPIHHVLMEAEKLVAAGVKELLVISQDTSAYGLDIRYKPETWRGSEYETRFLDLAKGLGSLGVW
TRMHYVYPYPHVDNVIPLMAEGLITPYLDIPFQHASAPVLKAMKRPGNQDKVLGRIAKWREQVPDLAIRSTFIVGFPGET
EEDFQILLDFIREAKIDRAGCFKYENVAHAESGKLEGHVPEEVKEERWHRFMTAQAEVSAARAEAQVGRTLDVIIDGPGE
EAGEMIARTQADAPEVDAVVYLENAAHLKAGDIIRAEITGADDYDLYATPA

Sequences:

>Translated_451_residues
MTLNLDRPAPLRPKTQPKVGIVSLGCPKALVDSERIITRLRAEGYQIAPDYAGADLVLVNTCGFLDSARDESLAAIGEAI
EANGKVIVTGCLGAEPEVIQRAHPKVLAVTGPHQYEAVMDAVHTHLTPPHNPHMDLVPESGLRLTPRHYAYLKISEGCNN
RCSFCIIPKLRGDLASRPIHHVLMEAEKLVAAGVKELLVISQDTSAYGLDIRYKPETWRGSEYETRFLDLAKGLGSLGVW
TRMHYVYPYPHVDNVIPLMAEGLITPYLDIPFQHASAPVLKAMKRPGNQDKVLGRIAKWREQVPDLAIRSTFIVGFPGET
EEDFQILLDFIREAKIDRAGCFKYENVAHAESGKLEGHVPEEVKEERWHRFMTAQAEVSAARAEAQVGRTLDVIIDGPGE
EAGEMIARTQADAPEVDAVVYLENAAHLKAGDIIRAEITGADDYDLYATPA
>Mature_450_residues
TLNLDRPAPLRPKTQPKVGIVSLGCPKALVDSERIITRLRAEGYQIAPDYAGADLVLVNTCGFLDSARDESLAAIGEAIE
ANGKVIVTGCLGAEPEVIQRAHPKVLAVTGPHQYEAVMDAVHTHLTPPHNPHMDLVPESGLRLTPRHYAYLKISEGCNNR
CSFCIIPKLRGDLASRPIHHVLMEAEKLVAAGVKELLVISQDTSAYGLDIRYKPETWRGSEYETRFLDLAKGLGSLGVWT
RMHYVYPYPHVDNVIPLMAEGLITPYLDIPFQHASAPVLKAMKRPGNQDKVLGRIAKWREQVPDLAIRSTFIVGFPGETE
EDFQILLDFIREAKIDRAGCFKYENVAHAESGKLEGHVPEEVKEERWHRFMTAQAEVSAARAEAQVGRTLDVIIDGPGEE
AGEMIARTQADAPEVDAVVYLENAAHLKAGDIIRAEITGADDYDLYATPA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=365, Percent_Identity=28.4931506849315, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=25.987525987526, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=26.1954261954262, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=62.0689655172414, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=27.8396436525612, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=419, Percent_Identity=23.8663484486874, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=189, Percent_Identity=31.2169312169312, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=23.6625514403292, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19922432, Length=333, Percent_Identity=29.4294294294294, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_HYPNA (Q0C0U1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760652.1
- ProteinModelPortal:   Q0C0U1
- SMR:   Q0C0U1
- STRING:   Q0C0U1
- GeneID:   4289303
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1951
- NMPDR:   fig|228405.5.peg.1880
- TIGR:   HNE_1951
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q0C0U1
- ProtClustDB:   PRK14862
- BioCyc:   HNEP81032:HNE_1951-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49672; Mature: 49541

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNLDRPAPLRPKTQPKVGIVSLGCPKALVDSERIITRLRAEGYQIAPDYAGADLVLVN
CEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEE
TCGFLDSARDESLAAIGEAIEANGKVIVTGCLGAEPEVIQRAHPKVLAVTGPHQYEAVMD
ECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHH
AVHTHLTPPHNPHMDLVPESGLRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLASRPIH
HHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHCHHHCCCHH
HVLMEAEKLVAAGVKELLVISQDTSAYGLDIRYKPETWRGSEYETRFLDLAKGLGSLGVW
HHHHHHHHHHHHCHHHEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHH
TRMHYVYPYPHVDNVIPLMAEGLITPYLDIPFQHASAPVLKAMKRPGNQDKVLGRIAKWR
HEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHH
EQVPDLAIRSTFIVGFPGETEEDFQILLDFIREAKIDRAGCFKYENVAHAESGKLEGHVP
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCC
EEVKEERWHRFMTAQAEVSAARAEAQVGRTLDVIIDGPGEEAGEMIARTQADAPEVDAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEE
YLENAAHLKAGDIIRAEITGADDYDLYATPA
EEECCCCCCCCCEEEEEECCCCCCCEEECCC
>Mature Secondary Structure 
TLNLDRPAPLRPKTQPKVGIVSLGCPKALVDSERIITRLRAEGYQIAPDYAGADLVLVN
EECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEE
TCGFLDSARDESLAAIGEAIEANGKVIVTGCLGAEPEVIQRAHPKVLAVTGPHQYEAVMD
ECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHH
AVHTHLTPPHNPHMDLVPESGLRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLASRPIH
HHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHCHHHCCCHH
HVLMEAEKLVAAGVKELLVISQDTSAYGLDIRYKPETWRGSEYETRFLDLAKGLGSLGVW
HHHHHHHHHHHHCHHHEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHH
TRMHYVYPYPHVDNVIPLMAEGLITPYLDIPFQHASAPVLKAMKRPGNQDKVLGRIAKWR
HEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHH
EQVPDLAIRSTFIVGFPGETEEDFQILLDFIREAKIDRAGCFKYENVAHAESGKLEGHVP
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCC
EEVKEERWHRFMTAQAEVSAARAEAQVGRTLDVIIDGPGEEAGEMIARTQADAPEVDAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEE
YLENAAHLKAGDIIRAEITGADDYDLYATPA
EEECCCCCCCCCEEEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA