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The map label for this gene is fadA [H]

Identifier: 114798652

GI number: 114798652

Start: 3312282

End: 3313457

Strand: Direct

Name: fadA [H]

Synonym: HNE_3133

Alternate gene names: 114798652

Gene position: 3312282-3313457 (Clockwise)

Preceding gene: 114798977

Following gene: 114797663

Centisome position: 89.4

GC content: 62.33

Gene sequence:

>1176_bases
ATGGCGACCGCATATATTGTAGCAGCAAATCGCACGGCCGGTGGCCGCAAGAACGGCGCGCTCGCCGAATGGCATCCCGG
CGATCTGGGCGCGCAAGTGCTCAACGCCATCGTCGCAGAGACCGGCGTTGACCCCAAGGCCATCGAAGACGTGATCATGG
GCTGCGTTTCGCAGGCCGGTGAACAGGCAGGCCAGATCGGCCGGACCGCCGTACTGGCCTCCAACCTTCCAAATTCCGTC
CCTGCCGTGACCATCGACCGCCAGTGCGGCTCGTCCCAGCAGTCGATCCAGTTCGCGGCGCAGGCCATCATGTCCGGCAC
GCAGGATCTCGTGATTGCGGCCGGCGTGGAAAGCATGACGCGCTGCCCGATGGGCATCAACGCCCGCGCCGGCAAGGTGT
TCGACATCCCGACCGATCCGACACCAAAATCGGTGAAGGAAAAATTCGGCATCAAACATTTCAGCCAGTTTGCCGGCGCC
GAAATGATCGCCAAAAAGCATGGCCATACCAAAGAACAACTCGACGCTTTCTCGGTCGAAAGCCACCGCCGGGCCGCCGC
TGCCACCAAGTCGGGCGCCTTCGCAAAAGAAATCGTCATCATCAAGGGCCGCAATGGCGAAGGCCAGGAAGTGATGCACG
ACAAGGATGAGGGCATCCGCTACGACGCCAGCATGGAAGGCATGGCGAGCCTCAAGATCCTCATGGAAGACGGCGTCATG
ACGGCCGCGAACGCCAGCCAGATCTGCGATGGCGCGTCGGCCGTTATGATTGCCTCGGAAGCGGCGATCAAAGCGCACAA
CCTCACCCCGCTGGCCCGCATTCACAATGTGACGGTCACTGCTGGCGATCCGGTCATCATGCTGGAAGAGCCGCTCTTCG
CGACCGACCGCGCCCTGCAACGCGCCGGCATGAAAATCGGCGATATCGACCTCTATGAAGTCAACGAGGCGTTCGCCCCT
GTGCCCCTCGCCTGGCTCAAGCATGTCGGCGCCGACCCATCGAAACTCAACGTCAATGGCGGCGCCATCTCGCTCGGCCA
CCCGCTGGGCGCATCGGGCACCAAGTTGATGGCAACGCTGGTTCATGCTCTGCGCGCGCGCGGTGGCAAATACGGCCTGC
AAACGATGTGTGAAGGCGGCGGCGTTGCCAACGTCACCATCATCGAAGCGCTCTGA

Upstream 100 bases:

>100_bases
GAAAAATTGCTCTTCGCGCTTTGCCCCTGCCCCAGCGTGACCGCTGGCGCAGGCCTGAACGCGCGTTAAAATCAGGCATC
CAGTCAGAAGGAACAAGACC

Downstream 100 bases:

>100_bases
TCCGATAACGGATGAAGTTCAAGAAACGCCGGGCGGCAAGGTCCGGCGTTTTTTGTTGCCCCTACTCCGTATCCAGTTCC
GGGTTGCGCACCTGGATAAG

Product: acetyl-CoA acyltransferase/thiolase family protein

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MATAYIVAANRTAGGRKNGALAEWHPGDLGAQVLNAIVAETGVDPKAIEDVIMGCVSQAGEQAGQIGRTAVLASNLPNSV
PAVTIDRQCGSSQQSIQFAAQAIMSGTQDLVIAAGVESMTRCPMGINARAGKVFDIPTDPTPKSVKEKFGIKHFSQFAGA
EMIAKKHGHTKEQLDAFSVESHRRAAAATKSGAFAKEIVIIKGRNGEGQEVMHDKDEGIRYDASMEGMASLKILMEDGVM
TAANASQICDGASAVMIASEAAIKAHNLTPLARIHNVTVTAGDPVIMLEEPLFATDRALQRAGMKIGDIDLYEVNEAFAP
VPLAWLKHVGADPSKLNVNGGAISLGHPLGASGTKLMATLVHALRARGGKYGLQTMCEGGGVANVTIIEAL

Sequences:

>Translated_391_residues
MATAYIVAANRTAGGRKNGALAEWHPGDLGAQVLNAIVAETGVDPKAIEDVIMGCVSQAGEQAGQIGRTAVLASNLPNSV
PAVTIDRQCGSSQQSIQFAAQAIMSGTQDLVIAAGVESMTRCPMGINARAGKVFDIPTDPTPKSVKEKFGIKHFSQFAGA
EMIAKKHGHTKEQLDAFSVESHRRAAAATKSGAFAKEIVIIKGRNGEGQEVMHDKDEGIRYDASMEGMASLKILMEDGVM
TAANASQICDGASAVMIASEAAIKAHNLTPLARIHNVTVTAGDPVIMLEEPLFATDRALQRAGMKIGDIDLYEVNEAFAP
VPLAWLKHVGADPSKLNVNGGAISLGHPLGASGTKLMATLVHALRARGGKYGLQTMCEGGGVANVTIIEAL
>Mature_390_residues
ATAYIVAANRTAGGRKNGALAEWHPGDLGAQVLNAIVAETGVDPKAIEDVIMGCVSQAGEQAGQIGRTAVLASNLPNSVP
AVTIDRQCGSSQQSIQFAAQAIMSGTQDLVIAAGVESMTRCPMGINARAGKVFDIPTDPTPKSVKEKFGIKHFSQFAGAE
MIAKKHGHTKEQLDAFSVESHRRAAAATKSGAFAKEIVIIKGRNGEGQEVMHDKDEGIRYDASMEGMASLKILMEDGVMT
AANASQICDGASAVMIASEAAIKAHNLTPLARIHNVTVTAGDPVIMLEEPLFATDRALQRAGMKIGDIDLYEVNEAFAPV
PLAWLKHVGADPSKLNVNGGAISLGHPLGASGTKLMATLVHALRARGGKYGLQTMCEGGGVANVTIIEAL

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=39.7959183673469, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=35.8778625954198, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI148539872, Length=385, Percent_Identity=37.4025974025974, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI4501853, Length=405, Percent_Identity=33.8271604938272, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4504327, Length=406, Percent_Identity=30.5418719211823, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=26.0869565217391, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787663, Length=406, Percent_Identity=40.3940886699507, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=40, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=37.1859296482412, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=40.1985111662531, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=31.4553990610329, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=385, Percent_Identity=37.6623376623377, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=34.2639593908629, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=32.1167883211679, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=30.3571428571429, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17551802, Length=404, Percent_Identity=29.2079207920792, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=36.75, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=32.5757575757576, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=36.2944162436548, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=35.4838709677419, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=37.6590330788804, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137578, Length=404, Percent_Identity=28.960396039604, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19921506, Length=373, Percent_Identity=22.5201072386059, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585051, Length=396, Percent_Identity=22.979797979798, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 40770; Mature: 40639

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAYIVAANRTAGGRKNGALAEWHPGDLGAQVLNAIVAETGVDPKAIEDVIMGCVSQAG
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQAGQIGRTAVLASNLPNSVPAVTIDRQCGSSQQSIQFAAQAIMSGTQDLVIAAGVESMT
HHHHHHCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHH
RCPMGINARAGKVFDIPTDPTPKSVKEKFGIKHFSQFAGAEMIAKKHGHTKEQLDAFSVE
HCCCCCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SHRRAAAATKSGAFAKEIVIIKGRNGEGQEVMHDKDEGIRYDASMEGMASLKILMEDGVM
HHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHCCCCCCEEECCCCCCHHHEEEEECCCEE
TAANASQICDGASAVMIASEAAIKAHNLTPLARIHNVTVTAGDPVIMLEEPLFATDRALQ
EECCHHHHCCCCCEEEEEHHHHHHHCCCCHHHHHEEEEEECCCCEEEECCCCHHHHHHHH
RAGMKIGDIDLYEVNEAFAPVPLAWLKHVGADPSKLNVNGGAISLGHPLGASGTKLMATL
HCCCEECCEEEEECCCCCCCCHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHHHH
VHALRARGGKYGLQTMCEGGGVANVTIIEAL
HHHHHHCCCCCCHHHHHCCCCEEEEEEEEEC
>Mature Secondary Structure 
ATAYIVAANRTAGGRKNGALAEWHPGDLGAQVLNAIVAETGVDPKAIEDVIMGCVSQAG
CEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQAGQIGRTAVLASNLPNSVPAVTIDRQCGSSQQSIQFAAQAIMSGTQDLVIAAGVESMT
HHHHHHCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHH
RCPMGINARAGKVFDIPTDPTPKSVKEKFGIKHFSQFAGAEMIAKKHGHTKEQLDAFSVE
HCCCCCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SHRRAAAATKSGAFAKEIVIIKGRNGEGQEVMHDKDEGIRYDASMEGMASLKILMEDGVM
HHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHCCCCCCEEECCCCCCHHHEEEEECCCEE
TAANASQICDGASAVMIASEAAIKAHNLTPLARIHNVTVTAGDPVIMLEEPLFATDRALQ
EECCHHHHCCCCCEEEEEHHHHHHHCCCCHHHHHEEEEEECCCCEEEECCCCHHHHHHHH
RAGMKIGDIDLYEVNEAFAPVPLAWLKHVGADPSKLNVNGGAISLGHPLGASGTKLMATL
HCCCEECCEEEEECCCCCCCCHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHHHH
VHALRARGGKYGLQTMCEGGGVANVTIIEAL
HHHHHHCCCCCCHHHHHCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]