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The map label for this gene is lip2 [H]

Identifier: 114798639

GI number: 114798639

Start: 152262

End: 153218

Strand: Reverse

Name: lip2 [H]

Synonym: HNE_0166

Alternate gene names: 114798639

Gene position: 153218-152262 (Counterclockwise)

Preceding gene: 114797895

Following gene: 114798938

Centisome position: 4.14

GC content: 59.35

Gene sequence:

>957_bases
ATGACGTTTGACGATGCCTTCCGCAGCTTCAGAGACAAACTGCACCTCCCAGAGATGCATCCACCGGACCTGCGCCTGCC
CATCGAGCGGCTGCGGCAACAGTTTTTCAGGATGGACTATTCTGGAGAACCCGACGCGCCGGAACTCGCAGACGTTCGCA
TTCTGACCATTCCCGGCCCCACCGGTCCCCTTAAGGCCCGCCTGTACGTACCCCTCGGCGCTGGCGTACCGCCCGGCCCC
GGCATACTTTTTTTCCATGGTGGCGGGTTTGTCCTGGGTGATCTGGAAAGCCATGACATGATCTGCCGCCGTATGGCAGA
AGGCTCGCGCTGCCGGGTGCTGGCAATCGATTATCGCCTCGCGCCGGAAACCTGCTTCCCATGCGCACATGACGACGCCC
TGGCCGCGTGGCACTGGATCAGCGCGCATGGTGAAGATGTCGGAATTGATCCGCGCCGTGTCGCCGTGGCCGGAGATAGT
GCTGGCGGAAACCTGGCTGCATTCGTCTGCCAACAGATGAACCGGATCAAAGGCCCCCGGCCCGCCTTCCAGCTACTTCT
CTACCCCCTGCTCCAGTTTGCCGACATCCGCAGCAAGAAGATGTCTCCTCAGGAGAGCGGCTTCTTCATCTCCGTCGGCC
TGTTCGAATTCTTCCGGGGCCACTATCTGCCCGATCCGGGAACTTACATGGACCCCCGAATTTCACCATTGTTTGCGCCC
GCTGAAGAACTGAAAGGTTTGCCCCCGGCACATATCATCGTGTGCGGATGGGACCCTCTGCATGATGAAGGTCTCGCCTA
TGCAGCAAAGCTGCGCGCCATGGGCATTGCGGTGAGTGAAAAGGAATATCCTTCGATGGTACACGGATTTCTGAACCTTA
CTCACATCTCAGCGACCGCACGGTTGGCAGCAAGAGAGGCGGGCAAAATAACGGGCGAAGCCCTCGGTGCGATTTAA

Upstream 100 bases:

>100_bases
CGGCGTCATACAACTTTAGATCCGGACGCGCTTTCAAGGCCCTTGCATCACACCTAATGCGCCATCACATCGCCGGAATT
GCCCGGCAGATGTGTGGCCC

Downstream 100 bases:

>100_bases
GAACTATATAAAGTTCCAAAAAAGTACTTACATTTTGCGTGACCCACGGGCAACACCCTGCAATTAAACACGAATATTGC
ACCGCAGCAAACATTTCAAA

Product: GDXG family lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTFDDAFRSFRDKLHLPEMHPPDLRLPIERLRQQFFRMDYSGEPDAPELADVRILTIPGPTGPLKARLYVPLGAGVPPGP
GILFFHGGGFVLGDLESHDMICRRMAEGSRCRVLAIDYRLAPETCFPCAHDDALAAWHWISAHGEDVGIDPRRVAVAGDS
AGGNLAAFVCQQMNRIKGPRPAFQLLLYPLLQFADIRSKKMSPQESGFFISVGLFEFFRGHYLPDPGTYMDPRISPLFAP
AEELKGLPPAHIIVCGWDPLHDEGLAYAAKLRAMGIAVSEKEYPSMVHGFLNLTHISATARLAAREAGKITGEALGAI

Sequences:

>Translated_318_residues
MTFDDAFRSFRDKLHLPEMHPPDLRLPIERLRQQFFRMDYSGEPDAPELADVRILTIPGPTGPLKARLYVPLGAGVPPGP
GILFFHGGGFVLGDLESHDMICRRMAEGSRCRVLAIDYRLAPETCFPCAHDDALAAWHWISAHGEDVGIDPRRVAVAGDS
AGGNLAAFVCQQMNRIKGPRPAFQLLLYPLLQFADIRSKKMSPQESGFFISVGLFEFFRGHYLPDPGTYMDPRISPLFAP
AEELKGLPPAHIIVCGWDPLHDEGLAYAAKLRAMGIAVSEKEYPSMVHGFLNLTHISATARLAAREAGKITGEALGAI
>Mature_317_residues
TFDDAFRSFRDKLHLPEMHPPDLRLPIERLRQQFFRMDYSGEPDAPELADVRILTIPGPTGPLKARLYVPLGAGVPPGPG
ILFFHGGGFVLGDLESHDMICRRMAEGSRCRVLAIDYRLAPETCFPCAHDDALAAWHWISAHGEDVGIDPRRVAVAGDSA
GGNLAAFVCQQMNRIKGPRPAFQLLLYPLLQFADIRSKKMSPQESGFFISVGLFEFFRGHYLPDPGTYMDPRISPLFAPA
EELKGLPPAHIIVCGWDPLHDEGLAYAAKLRAMGIAVSEKEYPSMVHGFLNLTHISATARLAAREAGKITGEALGAI

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=320, Percent_Identity=25.9375, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI206597554, Length=340, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157041239, Length=311, Percent_Identity=28.9389067524116, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI61966717, Length=302, Percent_Identity=27.8145695364238, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI157041237, Length=259, Percent_Identity=27.7992277992278, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI68051721, Length=287, Percent_Identity=25.0871080139373, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226423947, Length=295, Percent_Identity=24.7457627118644, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI21328446, Length=84, Percent_Identity=41.6666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=272, Percent_Identity=31.25, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996133, Length=320, Percent_Identity=26.5625, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17540028, Length=135, Percent_Identity=38.5185185185185, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=135, Percent_Identity=40.7407407407407, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567059, Length=124, Percent_Identity=40.3225806451613, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=116, Percent_Identity=40.5172413793103, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24656076, Length=116, Percent_Identity=40.5172413793103, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20130169, Length=116, Percent_Identity=40.5172413793103, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34954; Mature: 34823

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDDAFRSFRDKLHLPEMHPPDLRLPIERLRQQFFRMDYSGEPDAPELADVRILTIPGP
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
TGPLKARLYVPLGAGVPPGPGILFFHGGGFVLGDLESHDMICRRMAEGSRCRVLAIDYRL
CCCCEEEEEEEECCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHCCCCCEEEEEEEEEE
APETCFPCAHDDALAAWHWISAHGEDVGIDPRRVAVAGDSAGGNLAAFVCQQMNRIKGPR
CCCCCCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
PAFQLLLYPLLQFADIRSKKMSPQESGFFISVGLFEFFRGHYLPDPGTYMDPRISPLFAP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AEELKGLPPAHIIVCGWDPLHDEGLAYAAKLRAMGIAVSEKEYPSMVHGFLNLTHISATA
HHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHH
RLAAREAGKITGEALGAI
HHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
TFDDAFRSFRDKLHLPEMHPPDLRLPIERLRQQFFRMDYSGEPDAPELADVRILTIPGP
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
TGPLKARLYVPLGAGVPPGPGILFFHGGGFVLGDLESHDMICRRMAEGSRCRVLAIDYRL
CCCCEEEEEEEECCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHCCCCCEEEEEEEEEE
APETCFPCAHDDALAAWHWISAHGEDVGIDPRRVAVAGDSAGGNLAAFVCQQMNRIKGPR
CCCCCCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
PAFQLLLYPLLQFADIRSKKMSPQESGFFISVGLFEFFRGHYLPDPGTYMDPRISPLFAP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AEELKGLPPAHIIVCGWDPLHDEGLAYAAKLRAMGIAVSEKEYPSMVHGFLNLTHISATA
HHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHH
RLAAREAGKITGEALGAI
HHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]