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The map label for this gene is fadE25 [H]

Identifier: 114798420

GI number: 114798420

Start: 1600819

End: 1602084

Strand: Reverse

Name: fadE25 [H]

Synonym: HNE_1570

Alternate gene names: 114798420

Gene position: 1602084-1600819 (Counterclockwise)

Preceding gene: 114798040

Following gene: 114800441

Centisome position: 43.24

GC content: 55.85

Gene sequence:

>1266_bases
ATGCCGCATCTTTGGGTCCAACCCTGCACTGGCGCACTCCCCTTCGTAATACTACGGAGTGCTCGTTTATCGCTTTCAAG
TACGCTCCCTGTGAATGCTCAGTCACCGAGCGCCATCAGTACTGATAGACCAGACATGGCTATGTATTTGAGAGGCAATG
ACGGGTCAGGTTCCGACGCGGTAACAGCGCACTTCCGCGCTGAAGTGATCGATTTTCTCGACCGTGAGCTAACACCGGAA
ATGCGCATGGCAGGCCGCGCAACGACGGGCCTTAAATCAGCCCGCGACGCTTGTAAGGAATGGGCTGAGAAGCTGTATCA
CCGCGGTTGGGCTGCCCCACTTTGGCCAACGGCATTTGGCGGCGCTGGATGGAACGACGCACAGAGGCTGTACTTTGAAA
ATGCCTGCGCAGAACGCGACGCACCGATTGTTCAATCATCTGGAGTCCGTACGATCGGACCGCTGATCATTTCAGAAGGC
ACACCGGAACAACAAGCTCGCTACCTGCCTAAAATCCTCAGCGGAGATCATGAATGGTGCCAGGGCTTTTCCGAACCGCA
AGCCGGTTCTGATTTGTGCGCGCTTACGCTGCGGGCGAACCTTGACGGCGATTACTTCGTTCTGAGCGGCAGCAAGATCT
GGACAAGCTTTGCCAGCTACGCAACTCACATGTTTCTCCTCGCCAGAAGCGACCAGGAAAGCCTTGGAGGCGCCGGCTTG
GTATTCCTTCTCGTAGAGCTCGACCGGCCGGGCATCAGCATAAGACCGATCCGATTTATCGATGGCGAAGCGGAGACGAA
TGAGGTCTTTTTCGATCAGGTCAGAACCCCTGTAAACGACCGCATCGGTGACATAGGGGATGGCTGGAGCACCGCGAAGC
GTCTTATGGCTATAGCCAGATCCAACAATACAACCACTGGCCTGATACGCCGGGCGCTGCGCGCTGCCCTCAGATCGGTC
AGGTCGGTACCGGATCCTGATCCTGCCTTGCTGTTGCGGTTGTCAGACTACTCCATGCGTATTGATGCATTTGAAATGCT
GGAGAACAGGATCGGTTCGAGCGACATCCCCATCAACGCTGGCGCGGCTAGTTCTATGCTGAAACTCATTGCCACCGAGC
TTCATCAAGCCATCACAGAAGTCGGACTTGACGGTGACCCCGATGGGGACTTTGCGCTCGCCAAATACTTCGCCACTCGC
GCCGCCAGCATCTACTCGGGCACCAGCGAAATTCATCGCAATATCCTGTATCGCTCTCTGGTTTAG

Upstream 100 bases:

>100_bases
CGTATTTTTCAATATAAACAACTGGTTGCTCTGCGTTTTCTTTTGAAATGTATCTCACTTTGTATCCATTCTGTATCCCA
TTTTCGAACCTCTGAATTCG

Downstream 100 bases:

>100_bases
AGTAAGTTGCCCTGCATGCATTGGCGAAATAGCTGAAACATCAGTGCCGGATCATTGGCAAATGGCCGTCAGCAGACGAA
AAGCTAAGCCGGATCATCTC

Product: acyl-CoA dehydrogenase family protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPHLWVQPCTGALPFVILRSARLSLSSTLPVNAQSPSAISTDRPDMAMYLRGNDGSGSDAVTAHFRAEVIDFLDRELTPE
MRMAGRATTGLKSARDACKEWAEKLYHRGWAAPLWPTAFGGAGWNDAQRLYFENACAERDAPIVQSSGVRTIGPLIISEG
TPEQQARYLPKILSGDHEWCQGFSEPQAGSDLCALTLRANLDGDYFVLSGSKIWTSFASYATHMFLLARSDQESLGGAGL
VFLLVELDRPGISIRPIRFIDGEAETNEVFFDQVRTPVNDRIGDIGDGWSTAKRLMAIARSNNTTTGLIRRALRAALRSV
RSVPDPDPALLLRLSDYSMRIDAFEMLENRIGSSDIPINAGAASSMLKLIATELHQAITEVGLDGDPDGDFALAKYFATR
AASIYSGTSEIHRNILYRSLV

Sequences:

>Translated_421_residues
MPHLWVQPCTGALPFVILRSARLSLSSTLPVNAQSPSAISTDRPDMAMYLRGNDGSGSDAVTAHFRAEVIDFLDRELTPE
MRMAGRATTGLKSARDACKEWAEKLYHRGWAAPLWPTAFGGAGWNDAQRLYFENACAERDAPIVQSSGVRTIGPLIISEG
TPEQQARYLPKILSGDHEWCQGFSEPQAGSDLCALTLRANLDGDYFVLSGSKIWTSFASYATHMFLLARSDQESLGGAGL
VFLLVELDRPGISIRPIRFIDGEAETNEVFFDQVRTPVNDRIGDIGDGWSTAKRLMAIARSNNTTTGLIRRALRAALRSV
RSVPDPDPALLLRLSDYSMRIDAFEMLENRIGSSDIPINAGAASSMLKLIATELHQAITEVGLDGDPDGDFALAKYFATR
AASIYSGTSEIHRNILYRSLV
>Mature_420_residues
PHLWVQPCTGALPFVILRSARLSLSSTLPVNAQSPSAISTDRPDMAMYLRGNDGSGSDAVTAHFRAEVIDFLDRELTPEM
RMAGRATTGLKSARDACKEWAEKLYHRGWAAPLWPTAFGGAGWNDAQRLYFENACAERDAPIVQSSGVRTIGPLIISEGT
PEQQARYLPKILSGDHEWCQGFSEPQAGSDLCALTLRANLDGDYFVLSGSKIWTSFASYATHMFLLARSDQESLGGAGLV
FLLVELDRPGISIRPIRFIDGEAETNEVFFDQVRTPVNDRIGDIGDGWSTAKRLMAIARSNNTTTGLIRRALRAALRSVR
SVPDPDPALLLRLSDYSMRIDAFEMLENRIGSSDIPINAGAASSMLKLIATELHQAITEVGLDGDPDGDFALAKYFATRA
ASIYSGTSEIHRNILYRSLV

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI21361497, Length=322, Percent_Identity=25.776397515528, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4501859, Length=351, Percent_Identity=26.2108262108262, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI4557233, Length=329, Percent_Identity=28.2674772036474, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4557231, Length=393, Percent_Identity=24.1730279898219, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI187960098, Length=393, Percent_Identity=24.1730279898219, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI226958412, Length=230, Percent_Identity=23.4782608695652, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI7656849, Length=284, Percent_Identity=26.4084507042254, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI226958414, Length=195, Percent_Identity=23.5897435897436, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI76496475, Length=297, Percent_Identity=25.2525252525253, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI4557235, Length=297, Percent_Identity=25.2525252525253, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508101, Length=387, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538396, Length=392, Percent_Identity=25.5102040816327, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17570075, Length=393, Percent_Identity=24.4274809160305, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=289, Percent_Identity=26.2975778546713, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17569725, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71985184, Length=201, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534899, Length=353, Percent_Identity=24.929178470255, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563615, Length=194, Percent_Identity=29.3814432989691, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=201, Percent_Identity=28.3582089552239, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71990804, Length=209, Percent_Identity=28.2296650717703, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363737, Length=213, Percent_Identity=29.1079812206573, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355753, Length=254, Percent_Identity=27.5590551181102, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666513, Length=304, Percent_Identity=24.6710526315789, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24646207, Length=370, Percent_Identity=26.7567567567568, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24660351, Length=289, Percent_Identity=26.643598615917, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356377, Length=288, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 46031; Mature: 45899

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLWVQPCTGALPFVILRSARLSLSSTLPVNAQSPSAISTDRPDMAMYLRGNDGSGSDA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCH
VTAHFRAEVIDFLDRELTPEMRMAGRATTGLKSARDACKEWAEKLYHRGWAAPLWPTAFG
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GAGWNDAQRLYFENACAERDAPIVQSSGVRTIGPLIISEGTPEQQARYLPKILSGDHEWC
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEECCCCCHHHHHHHHHHHCCCHHHH
QGFSEPQAGSDLCALTLRANLDGDYFVLSGSKIWTSFASYATHMFLLARSDQESLGGAGL
CCCCCCCCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHEEECCCHHHCCCCCE
VFLLVELDRPGISIRPIRFIDGEAETNEVFFDQVRTPVNDRIGDIGDGWSTAKRLMAIAR
EEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHH
SNNTTTGLIRRALRAALRSVRSVPDPDPALLLRLSDYSMRIDAFEMLENRIGSSDIPINA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCC
GAASSMLKLIATELHQAITEVGLDGDPDGDFALAKYFATRAASIYSGTSEIHRNILYRSL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
V
C
>Mature Secondary Structure 
PHLWVQPCTGALPFVILRSARLSLSSTLPVNAQSPSAISTDRPDMAMYLRGNDGSGSDA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCH
VTAHFRAEVIDFLDRELTPEMRMAGRATTGLKSARDACKEWAEKLYHRGWAAPLWPTAFG
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GAGWNDAQRLYFENACAERDAPIVQSSGVRTIGPLIISEGTPEQQARYLPKILSGDHEWC
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEECCCCCHHHHHHHHHHHCCCHHHH
QGFSEPQAGSDLCALTLRANLDGDYFVLSGSKIWTSFASYATHMFLLARSDQESLGGAGL
CCCCCCCCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHEEECCCHHHCCCCCE
VFLLVELDRPGISIRPIRFIDGEAETNEVFFDQVRTPVNDRIGDIGDGWSTAKRLMAIAR
EEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHH
SNNTTTGLIRRALRAALRSVRSVPDPDPALLLRLSDYSMRIDAFEMLENRIGSSDIPINA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCC
GAASSMLKLIATELHQAITEVGLDGDPDGDFALAKYFATRAASIYSGTSEIHRNILYRSL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]