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The map label for this gene is btuB [H]

Identifier: 114798415

GI number: 114798415

Start: 1548377

End: 1550224

Strand: Direct

Name: btuB [H]

Synonym: HNE_1514

Alternate gene names: 114798415

Gene position: 1548377-1550224 (Clockwise)

Preceding gene: 114800288

Following gene: 114799541

Centisome position: 41.79

GC content: 57.2

Gene sequence:

>1848_bases
ATGTCGTATAAAACGGCACTCATCAGCGTGGCCGTCATGGCCGCGAGTGCATCCGCTTTTGCCCAAATCTCTGAGACTGA
AGAAAACCGGCTCAGATCGGTCGTCGTCGAGGGGTCGCGCCTCGACCAGACCGCCACCGAGGTCGGCTCCAGCGTCAGCA
TTATCGGCAAGGAAGACCTTGAAAAGCTGGACCTGGACTTCGCGTTAGATGCCGTGGCGACGGCGCCGGGCGTGACGATC
AACCAGAATGGCGCCTTTGGTGGCTCTGCCAGTGTCCGCATTCGAGGAGCATCCTCGGGCCAGACTCTGGTTCTCATTGA
TGGCGTTCCTGTCGGCGATCCGTCGACGACCGATGGCAGCTTCAATTTCGCCTATCTCGACACAGCCAATGTCGAGCGGA
TCGAAGTCCTTAAGGGGCCTCAGTCCACCCTCTGGGGCTCAAATGCCATAGGCGGCGTGGTCTCCGTCACTTCCCGCCAA
CCAAAGACCGGGTTCGGTGGCAGCGCATTTGCCGAATACGGATCGTTCAACACGCTCCGGGGCGGCGCATCCATTGACGG
CGCGAACGAGACTGGAGATTTCCGGCTTTCGGCAACCGGCATCTCGACTGACGGCATTTCAAAATCCGACAAAGAGAATG
GCAACACCGAAGAGGATGCCTACGACTCAACCACAGTGTCAGCGAAAGGTGGCCTGAACCTTCCCAACTCGGCCAGACTG
CAGGCAACGATCCTCCATAACCAGGCTGAGTCAGAATATGACAGCTTCTCCTCGGGTGCGCAGGGAAGCGTCGCCGATGG
GGATGAGGTTACCGAGAACGAAACCCTGTCGGGCAACATTTCGCTCAATCTTCCGCTCCTGGATGATCGGTTCGAGAACC
ATTTCCTCATCGGATATTCCGATATCACTCGCGAGAACTTCACCAATGGCGCGCAAAGCTACTTTGCTGAAGGCGACAGG
ACACTCTACCGCTATCAGGGCACCTTCGCGATAAATGACGCAAACAAGGTGTCCTTTGGCGCAGAGCGTGAAGAGACCTC
CGCCAACGGAGACGAGGCCAGCATTGACGGGCTGTTTGCCCTGTACGAGCTCAAACCGGTCGAAACCCTGACGCTGACCG
CAGGCTTACGCCAGGACGACGACAATCGGTTTGGTTCGGAGACAACGGCCCGAGTGGCCGCCGCGTGGAACGTAACCGGC
CATGTGACGATCCGCTCCAGCTGGGGCCAGGGCTTCAAGGCGCCTTCGCTATTCCAGACGACTTACATCTGTACCTTCTG
TGGCCTGAGCGAGCCGAATGTAGACCTCATGCCCGAGACGTCCGAAGCTTTCGATATCGGACTGGAGTGGCGTTCAGGGG
ATGGGCGCGCAGAAGCAGGTGTTACCTATTTCGATCAGAACACTGACAACCTTATCGACTTTTCCTACACTGCAGGTTAT
GACAACATTGCGCGTGTCGAAAGCAAGGGCGCCGAAGTGTTTGCCGGCTACGTTCTTACGAACTGGCTCAGCGTTGACGG
CAATTACACCTACATCGACGCGCACGACGGTGACGGCAACGAACTCACCCGTCTACCGAAACATTCCGCCAATTTTTCAC
TTACTGTCGATCCGGATGGTCCGTTCTCCGGAGCGGTGCTGGTTCGATATAACGACGATGAGGCCAATACAAATGGCACG
ACACTGGATGGATGGACGCGCGTCGACCTGACGGGACGTTATACGCTCAATGAACGCGTTGAACTGTATGGCCGTATCGA
AAACCTGTTTGATGAGGATTATCAGCAGATCCTCGGCTACGGCACGCCCGGCCTTTCAGGCTCGGTTGGCATCCGCCTGC
GCTACTAG

Upstream 100 bases:

>100_bases
ATTGGCTGGCCGCCCGCATGAGAGACCTCATGGGCCAGATGACGTCGTTATGAATTGCACCCATCTTTCCGGGCGGCAGG
GTGCATTGAGGGGAATACCC

Downstream 100 bases:

>100_bases
GAGTAGCTGATGTCGAGACTGGCGATCTTCTCTCTTCTGGCGTTCGGACTTTCAGCCTGCGGTGCTCCGCCCAAGCCGCA
GACAGATAGAGCTGACCGCC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSYKTALISVAVMAASASAFAQISETEENRLRSVVVEGSRLDQTATEVGSSVSIIGKEDLEKLDLDFALDAVATAPGVTI
NQNGAFGGSASVRIRGASSGQTLVLIDGVPVGDPSTTDGSFNFAYLDTANVERIEVLKGPQSTLWGSNAIGGVVSVTSRQ
PKTGFGGSAFAEYGSFNTLRGGASIDGANETGDFRLSATGISTDGISKSDKENGNTEEDAYDSTTVSAKGGLNLPNSARL
QATILHNQAESEYDSFSSGAQGSVADGDEVTENETLSGNISLNLPLLDDRFENHFLIGYSDITRENFTNGAQSYFAEGDR
TLYRYQGTFAINDANKVSFGAEREETSANGDEASIDGLFALYELKPVETLTLTAGLRQDDDNRFGSETTARVAAAWNVTG
HVTIRSSWGQGFKAPSLFQTTYICTFCGLSEPNVDLMPETSEAFDIGLEWRSGDGRAEAGVTYFDQNTDNLIDFSYTAGY
DNIARVESKGAEVFAGYVLTNWLSVDGNYTYIDAHDGDGNELTRLPKHSANFSLTVDPDGPFSGAVLVRYNDDEANTNGT
TLDGWTRVDLTGRYTLNERVELYGRIENLFDEDYQQILGYGTPGLSGSVGIRLRY

Sequences:

>Translated_615_residues
MSYKTALISVAVMAASASAFAQISETEENRLRSVVVEGSRLDQTATEVGSSVSIIGKEDLEKLDLDFALDAVATAPGVTI
NQNGAFGGSASVRIRGASSGQTLVLIDGVPVGDPSTTDGSFNFAYLDTANVERIEVLKGPQSTLWGSNAIGGVVSVTSRQ
PKTGFGGSAFAEYGSFNTLRGGASIDGANETGDFRLSATGISTDGISKSDKENGNTEEDAYDSTTVSAKGGLNLPNSARL
QATILHNQAESEYDSFSSGAQGSVADGDEVTENETLSGNISLNLPLLDDRFENHFLIGYSDITRENFTNGAQSYFAEGDR
TLYRYQGTFAINDANKVSFGAEREETSANGDEASIDGLFALYELKPVETLTLTAGLRQDDDNRFGSETTARVAAAWNVTG
HVTIRSSWGQGFKAPSLFQTTYICTFCGLSEPNVDLMPETSEAFDIGLEWRSGDGRAEAGVTYFDQNTDNLIDFSYTAGY
DNIARVESKGAEVFAGYVLTNWLSVDGNYTYIDAHDGDGNELTRLPKHSANFSLTVDPDGPFSGAVLVRYNDDEANTNGT
TLDGWTRVDLTGRYTLNERVELYGRIENLFDEDYQQILGYGTPGLSGSVGIRLRY
>Mature_614_residues
SYKTALISVAVMAASASAFAQISETEENRLRSVVVEGSRLDQTATEVGSSVSIIGKEDLEKLDLDFALDAVATAPGVTIN
QNGAFGGSASVRIRGASSGQTLVLIDGVPVGDPSTTDGSFNFAYLDTANVERIEVLKGPQSTLWGSNAIGGVVSVTSRQP
KTGFGGSAFAEYGSFNTLRGGASIDGANETGDFRLSATGISTDGISKSDKENGNTEEDAYDSTTVSAKGGLNLPNSARLQ
ATILHNQAESEYDSFSSGAQGSVADGDEVTENETLSGNISLNLPLLDDRFENHFLIGYSDITRENFTNGAQSYFAEGDRT
LYRYQGTFAINDANKVSFGAEREETSANGDEASIDGLFALYELKPVETLTLTAGLRQDDDNRFGSETTARVAAAWNVTGH
VTIRSSWGQGFKAPSLFQTTYICTFCGLSEPNVDLMPETSEAFDIGLEWRSGDGRAEAGVTYFDQNTDNLIDFSYTAGYD
NIARVESKGAEVFAGYVLTNWLSVDGNYTYIDAHDGDGNELTRLPKHSANFSLTVDPDGPFSGAVLVRYNDDEANTNGTT
LDGWTRVDLTGRYTLNERVELYGRIENLFDEDYQQILGYGTPGLSGSVGIRLRY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=176, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788478, Length=225, Percent_Identity=31.5555555555556, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 65870; Mature: 65739

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKTALISVAVMAASASAFAQISETEENRLRSVVVEGSRLDQTATEVGSSVSIIGKEDL
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHCCCEEEEEEHHHH
EKLDLDFALDAVATAPGVTINQNGAFGGSASVRIRGASSGQTLVLIDGVPVGDPSTTDGS
HHHCHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
FNFAYLDTANVERIEVLKGPQSTLWGSNAIGGVVSVTSRQPKTGFGGSAFAEYGSFNTLR
EEEEEEECCCCEEEEEECCCCHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEC
GGASIDGANETGDFRLSATGISTDGISKSDKENGNTEEDAYDSTTVSAKGGLNLPNSARL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEE
QATILHNQAESEYDSFSSGAQGSVADGDEVTENETLSGNISLNLPLLDDRFENHFLIGYS
EEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCEEECEEEEEECEECCCCCCEEEEEEH
DITRENFTNGAQSYFAEGDRTLYRYQGTFAINDANKVSFGAEREETSANGDEASIDGLFA
HHCHHHCCCHHHHHHCCCCCEEEEEECEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEE
LYELKPVETLTLTAGLRQDDDNRFGSETTARVAAAWNVTGHVTIRSSWGQGFKAPSLFQT
EEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHH
TYICTFCGLSEPNVDLMPETSEAFDIGLEWRSGDGRAEAGVTYFDQNTDNLIDFSYTAGY
EEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCC
DNIARVESKGAEVFAGYVLTNWLSVDGNYTYIDAHDGDGNELTRLPKHSANFSLTVDPDG
CCHHHHHCCCCEEEEEEEEEEEEEECCCEEEEECCCCCCCHHHCCCCCCCCEEEEECCCC
PFSGAVLVRYNDDEANTNGTTLDGWTRVDLTGRYTLNERVELYGRIENLFDEDYQQILGY
CCCCEEEEEECCCCCCCCCCEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GTPGLSGSVGIRLRY
CCCCCCCCEEEEEEC
>Mature Secondary Structure 
SYKTALISVAVMAASASAFAQISETEENRLRSVVVEGSRLDQTATEVGSSVSIIGKEDL
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHCCCEEEEEEHHHH
EKLDLDFALDAVATAPGVTINQNGAFGGSASVRIRGASSGQTLVLIDGVPVGDPSTTDGS
HHHCHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
FNFAYLDTANVERIEVLKGPQSTLWGSNAIGGVVSVTSRQPKTGFGGSAFAEYGSFNTLR
EEEEEEECCCCEEEEEECCCCHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEC
GGASIDGANETGDFRLSATGISTDGISKSDKENGNTEEDAYDSTTVSAKGGLNLPNSARL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEE
QATILHNQAESEYDSFSSGAQGSVADGDEVTENETLSGNISLNLPLLDDRFENHFLIGYS
EEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCEEECEEEEEECEECCCCCCEEEEEEH
DITRENFTNGAQSYFAEGDRTLYRYQGTFAINDANKVSFGAEREETSANGDEASIDGLFA
HHCHHHCCCHHHHHHCCCCCEEEEEECEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEE
LYELKPVETLTLTAGLRQDDDNRFGSETTARVAAAWNVTGHVTIRSSWGQGFKAPSLFQT
EEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHH
TYICTFCGLSEPNVDLMPETSEAFDIGLEWRSGDGRAEAGVTYFDQNTDNLIDFSYTAGY
EEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCC
DNIARVESKGAEVFAGYVLTNWLSVDGNYTYIDAHDGDGNELTRLPKHSANFSLTVDPDG
CCHHHHHCCCCEEEEEEEEEEEEEECCCEEEEECCCCCCCHHHCCCCCCCCEEEEECCCC
PFSGAVLVRYNDDEANTNGTTLDGWTRVDLTGRYTLNERVELYGRIENLFDEDYQQILGY
CCCCEEEEEECCCCCCCCCCEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GTPGLSGSVGIRLRY
CCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA