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The map label for this gene is metF [H]

Identifier: 114798390

GI number: 114798390

Start: 2179039

End: 2179953

Strand: Reverse

Name: metF [H]

Synonym: HNE_2103

Alternate gene names: 114798390

Gene position: 2179953-2179039 (Counterclockwise)

Preceding gene: 114799579

Following gene: 114797600

Centisome position: 58.84

GC content: 64.37

Gene sequence:

>915_bases
GTGACCCGTCCGGCCATCCCGTCGCAAGCAACTAAAGACCCGGTGCGGGTCTCGTTCGAGTTTTTCCCGCCGAAATCCGA
GGCGATGAATGCCCGCCTCTGGGAGACGGTTCAGCGCCTGGAGCCGATGGCGCCGGCATTTGTCTCGGTCACCTATGGCG
CGGGCGGCTCCACGCGGGAGCGCACGCATGACACGGTGCGCCGCATCGCGGCGGAAACGGCGCTTCCCCCGGCAGCGCAC
CTGACCTGCGTTTCGGCCACGAAGGAAGAAGTGCTCGCCGTAGCCGAGGAATATTGGCAGGCGGGCGTGAAGCATATCGT
TGCGCTGCGCGGCGACCCGCCAGCAGGCATGGGCGCGAAGTTCGAGGCCCATCCGGGAGGCTTTGCCGACTCGGTTGATC
TCATCCGTGGGCTGAGGGAGCATCGCCCGGAGCTGGGCAAGTGTTTCGAGATTTCGGTCTCCTGCTATCCTGAGCTTCAC
CCGGAAAATCTGGGATGGGACGCGGAGATTGCCTTCCTGAAGGCCAAGCAGGATGCGGGCGCGGACCGGGCGATCACGCA
ATTCTTCTTCGAGCCGGACGTGTATCTCGCTTTCCTCGAGAAGGCGCGGGCAGGCGGCGTGACCATGCCGATCGTTCCCG
GCATCATGCTGCAGCCCAATTTCAACGGCCTGAAGCGCATCGCTGGCCTGTGCGGTTCATCCATCCCCGCCTGGCTGCAC
ACGCTTTATGACGGGCTGGATGAGGACGAGGAGACACGTGATCTCGTCACCGCGAATGTGGCCGCCGACCTCTGCCGGAA
ACTGCAGGCCGAAGGTGTTGAGGACTTCCATTTCTACACGCTGAACCGCGCGGGGCTGGCGCTGTCCACCTGCCGCCTGC
TTGGCCTCAACCCCCAGTCCGCAAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
AGAAACCCTTGAGCTTTGCGCCCCCGGAAAATGGCGCGCCGGGCCTTACCGTCAATATCTGGCGGGCGTTGAAGCCCGCC
TCAGTTCAGGAGAAAGCCGC

Downstream 100 bases:

>100_bases
GCCATGAACAGCCAAGACCGTTTCAAGGCGCTCGAAGCGCTCGCCAATCAACGTATCCTCGTGCTCGACGGCGCGATGGG
CACGCAGATTCAGAACTACA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTRPAIPSQATKDPVRVSFEFFPPKSEAMNARLWETVQRLEPMAPAFVSVTYGAGGSTRERTHDTVRRIAAETALPPAAH
LTCVSATKEEVLAVAEEYWQAGVKHIVALRGDPPAGMGAKFEAHPGGFADSVDLIRGLREHRPELGKCFEISVSCYPELH
PENLGWDAEIAFLKAKQDAGADRAITQFFFEPDVYLAFLEKARAGGVTMPIVPGIMLQPNFNGLKRIAGLCGSSIPAWLH
TLYDGLDEDEETRDLVTANVAADLCRKLQAEGVEDFHFYTLNRAGLALSTCRLLGLNPQSAKAA

Sequences:

>Translated_304_residues
MTRPAIPSQATKDPVRVSFEFFPPKSEAMNARLWETVQRLEPMAPAFVSVTYGAGGSTRERTHDTVRRIAAETALPPAAH
LTCVSATKEEVLAVAEEYWQAGVKHIVALRGDPPAGMGAKFEAHPGGFADSVDLIRGLREHRPELGKCFEISVSCYPELH
PENLGWDAEIAFLKAKQDAGADRAITQFFFEPDVYLAFLEKARAGGVTMPIVPGIMLQPNFNGLKRIAGLCGSSIPAWLH
TLYDGLDEDEETRDLVTANVAADLCRKLQAEGVEDFHFYTLNRAGLALSTCRLLGLNPQSAKAA
>Mature_303_residues
TRPAIPSQATKDPVRVSFEFFPPKSEAMNARLWETVQRLEPMAPAFVSVTYGAGGSTRERTHDTVRRIAAETALPPAAHL
TCVSATKEEVLAVAEEYWQAGVKHIVALRGDPPAGMGAKFEAHPGGFADSVDLIRGLREHRPELGKCFEISVSCYPELHP
ENLGWDAEIAFLKAKQDAGADRAITQFFFEPDVYLAFLEKARAGGVTMPIVPGIMLQPNFNGLKRIAGLCGSSIPAWLHT
LYDGLDEDEETRDLVTANVAADLCRKLQAEGVEDFHFYTLNRAGLALSTCRLLGLNPQSAKAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=33.0985915492958, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1790377, Length=284, Percent_Identity=46.4788732394366, Blast_Score=271, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI25147436, Length=285, Percent_Identity=32.6315789473684, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=32.6315789473684, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325234, Length=280, Percent_Identity=32.8571428571429, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=291, Percent_Identity=31.2714776632302, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24662658, Length=229, Percent_Identity=28.82096069869, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33134; Mature: 33003

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPAIPSQATKDPVRVSFEFFPPKSEAMNARLWETVQRLEPMAPAFVSVTYGAGGSTRE
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
RTHDTVRRIAAETALPPAAHLTCVSATKEEVLAVAEEYWQAGVKHIVALRGDPPAGMGAK
HHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCE
FEAHPGGFADSVDLIRGLREHRPELGKCFEISVSCYPELHPENLGWDAEIAFLKAKQDAG
EECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCC
ADRAITQFFFEPDVYLAFLEKARAGGVTMPIVPGIMLQPNFNGLKRIAGLCGSSIPAWLH
CHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCCCEEECCCHHHHHHHHHHHCCCHHHHHH
TLYDGLDEDEETRDLVTANVAADLCRKLQAEGVEDFHFYTLNRAGLALSTCRLLGLNPQS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCC
AKAA
CCCC
>Mature Secondary Structure 
TRPAIPSQATKDPVRVSFEFFPPKSEAMNARLWETVQRLEPMAPAFVSVTYGAGGSTRE
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
RTHDTVRRIAAETALPPAAHLTCVSATKEEVLAVAEEYWQAGVKHIVALRGDPPAGMGAK
HHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCE
FEAHPGGFADSVDLIRGLREHRPELGKCFEISVSCYPELHPENLGWDAEIAFLKAKQDAG
EECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCC
ADRAITQFFFEPDVYLAFLEKARAGGVTMPIVPGIMLQPNFNGLKRIAGLCGSSIPAWLH
CHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCCCEEECCCHHHHHHHHHHHCCCHHHHHH
TLYDGLDEDEETRDLVTANVAADLCRKLQAEGVEDFHFYTLNRAGLALSTCRLLGLNPQS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCC
AKAA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]