Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 114797600

GI number: 114797600

Start: 2177977

End: 2179035

Strand: Reverse

Name: metH [H]

Synonym: HNE_2102

Alternate gene names: 114797600

Gene position: 2179035-2177977 (Counterclockwise)

Preceding gene: 114798390

Following gene: 114800042

Centisome position: 58.81

GC content: 61.0

Gene sequence:

>1059_bases
ATGAACAGCCAAGACCGTTTCAAGGCGCTCGAAGCGCTCGCCAATCAACGTATCCTCGTGCTCGACGGCGCGATGGGCAC
GCAGATTCAGAACTACAAGCTGACCGAAGAGGATTTTCAGGGAAACCGGTTTGGCGACTGGGAAATTCCGTTGAAGGGCA
ACAATGACCTTTTGAACCTCACCCGCCCGGATGTGATCGGCGAGATTCACCGTAAATATATTGATGCGGGGGCGGACTTT
GTGGAGACGAACACATTCTCTGCGACCACCATCGCCATGGCCGATTACAAGATGGAGGCCCTTGCCGCCGAAATCGCCCG
CGAAGGCGCGCGGATTGCGCGGGATGTGGCTGATAAGGCCGAAGCAGAACTTGGCCGTCCTGTAGGCGTGATGGGCGCTA
TCGGGCCGACGAACAAGACGCTGTCGCTGAGCCCGAATGTGAATGATCCGGGCTACCGGGACGTGACATTTGACCAGGTG
CGCGAAGCCTATTTTGAGCAGGCCGAGGCGATGGCGCCGTTTATAGACGTGTTCCTGATCGAGACGGTGTTCGACACACT
GAACGCCAAGGCGGCGATCAAGGCGCTGATCGACCTGCGCGAGCAGAAGGGTATCGACCTGCCGATCATCATTTCCGGCA
CGATTACGGATGCTTCGGGCCGGACGCTCAGTGGGCAGACGGCTGAGGCGTTCTGGAATTCCGTGCGCCATGCGCGGCCC
TGGGCGATCGGCCTCAATTGCGCGCTGGGCGCGGACCTGATGCGCCAGCACGTGGCCTCTATTTCGCGCGTGGCGGATAC
CCGCGTGATTGCTTATCCCAATGCCGGCCTGCCCAACGCCTTTGGCGAGTATGACGAGACGCCTGACCAGACCGCGGCGC
ATCTGAAAGAATGGGCCAACAGCGGGCTGGTGAACGTGCTGGGCGGATGCTGCGGCACCTCGCCGGGACATATCGCTGCG
ATTGCGCAGGCAATCAGATCGGCGCCGCCGCGCAAGCTGCCGGAAGTTTCGCGGGCCATGCGTCTGTCGGGGCTGGAGCC
GTTTACGGTGGCATCGTGA

Upstream 100 bases:

>100_bases
TGAGGACTTCCATTTCTACACGCTGAACCGCGCGGGGCTGGCGCTGTCCACCTGCCGCCTGCTTGGCCTCAACCCCCAGT
CCGCAAAGGCAGCCTGAGCC

Downstream 100 bases:

>100_bases
CGCAAACGGACGTGCAGGCCGGAGATGTTTTCATCTTCTACGGCCTTCTGAAGCAGGGCGCAGCGGGCGCGCCGGACGAG
CTCGGACTTGAGCTGGCCGG

Product: putative metionine synthase, homocysteine S-methyltransferase subunit

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNSQDRFKALEALANQRILVLDGAMGTQIQNYKLTEEDFQGNRFGDWEIPLKGNNDLLNLTRPDVIGEIHRKYIDAGADF
VETNTFSATTIAMADYKMEALAAEIAREGARIARDVADKAEAELGRPVGVMGAIGPTNKTLSLSPNVNDPGYRDVTFDQV
REAYFEQAEAMAPFIDVFLIETVFDTLNAKAAIKALIDLREQKGIDLPIIISGTITDASGRTLSGQTAEAFWNSVRHARP
WAIGLNCALGADLMRQHVASISRVADTRVIAYPNAGLPNAFGEYDETPDQTAAHLKEWANSGLVNVLGGCCGTSPGHIAA
IAQAIRSAPPRKLPEVSRAMRLSGLEPFTVAS

Sequences:

>Translated_352_residues
MNSQDRFKALEALANQRILVLDGAMGTQIQNYKLTEEDFQGNRFGDWEIPLKGNNDLLNLTRPDVIGEIHRKYIDAGADF
VETNTFSATTIAMADYKMEALAAEIAREGARIARDVADKAEAELGRPVGVMGAIGPTNKTLSLSPNVNDPGYRDVTFDQV
REAYFEQAEAMAPFIDVFLIETVFDTLNAKAAIKALIDLREQKGIDLPIIISGTITDASGRTLSGQTAEAFWNSVRHARP
WAIGLNCALGADLMRQHVASISRVADTRVIAYPNAGLPNAFGEYDETPDQTAAHLKEWANSGLVNVLGGCCGTSPGHIAA
IAQAIRSAPPRKLPEVSRAMRLSGLEPFTVAS
>Mature_352_residues
MNSQDRFKALEALANQRILVLDGAMGTQIQNYKLTEEDFQGNRFGDWEIPLKGNNDLLNLTRPDVIGEIHRKYIDAGADF
VETNTFSATTIAMADYKMEALAAEIAREGARIARDVADKAEAELGRPVGVMGAIGPTNKTLSLSPNVNDPGYRDVTFDQV
REAYFEQAEAMAPFIDVFLIETVFDTLNAKAAIKALIDLREQKGIDLPIIISGTITDASGRTLSGQTAEAFWNSVRHARP
WAIGLNCALGADLMRQHVASISRVADTRVIAYPNAGLPNAFGEYDETPDQTAAHLKEWANSGLVNVLGGCCGTSPGHIAA
IAQAIRSAPPRKLPEVSRAMRLSGLEPFTVAS

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=345, Percent_Identity=53.9130434782609, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1790450, Length=347, Percent_Identity=57.6368876080692, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535405, Length=354, Percent_Identity=53.3898305084746, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38101; Mature: 38101

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQDRFKALEALANQRILVLDGAMGTQIQNYKLTEEDFQGNRFGDWEIPLKGNNDLLNL
CCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCEEEC
TRPDVIGEIHRKYIDAGADFVETNTFSATTIAMADYKMEALAAEIAREGARIARDVADKA
CCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAELGRPVGVMGAIGPTNKTLSLSPNVNDPGYRDVTFDQVREAYFEQAEAMAPFIDVFLI
HHHHCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETVFDTLNAKAAIKALIDLREQKGIDLPIIISGTITDASGRTLSGQTAEAFWNSVRHARP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
WAIGLNCALGADLMRQHVASISRVADTRVIAYPNAGLPNAFGEYDETPDQTAAHLKEWAN
CEEECHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHC
SGLVNVLGGCCGTSPGHIAAIAQAIRSAPPRKLPEVSRAMRLSGLEPFTVAS
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNSQDRFKALEALANQRILVLDGAMGTQIQNYKLTEEDFQGNRFGDWEIPLKGNNDLLNL
CCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCEEEC
TRPDVIGEIHRKYIDAGADFVETNTFSATTIAMADYKMEALAAEIAREGARIARDVADKA
CCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAELGRPVGVMGAIGPTNKTLSLSPNVNDPGYRDVTFDQVREAYFEQAEAMAPFIDVFLI
HHHHCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETVFDTLNAKAAIKALIDLREQKGIDLPIIISGTITDASGRTLSGQTAEAFWNSVRHARP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
WAIGLNCALGADLMRQHVASISRVADTRVIAYPNAGLPNAFGEYDETPDQTAAHLKEWAN
CEEECHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHC
SGLVNVLGGCCGTSPGHIAAIAQAIRSAPPRKLPEVSRAMRLSGLEPFTVAS
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]