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The map label for this gene is ubiF [H]

Identifier: 114798374

GI number: 114798374

Start: 3680977

End: 3682185

Strand: Direct

Name: ubiF [H]

Synonym: HNE_3543

Alternate gene names: 114798374

Gene position: 3680977-3682185 (Clockwise)

Preceding gene: 114798332

Following gene: 114798962

Centisome position: 99.35

GC content: 67.33

Gene sequence:

>1209_bases
ATGGAGGCTGACCTCGCCGTCATCGGTGCCGGGCCCGTCGGCACATCGCTGGCGGTGCTTGCCGCGAAAGCAGGCCTGCG
TACCGTGCTGGTCGACATGCGGCCGGAGAATGCCGGGCCCGCAGCAGACACACGCAGCTTCGCGATTGTGCGGGGCAGCT
GGCGCCTCCTCGGCGCAGCCGGGGTTCACAGCGATCTGGCGGGCCTGACCGAGCCCCTCAACGGGCTGGAAGCGATGGAT
GGGGGCACCCACTGGTTTGGTGCGCCGTGGGCGGCCTTTTCCGCAACGGATCTGCCCGGCGATGATCCCCAGCCGCTGGG
CGAACTTGTGCCTGCCGGCGCGCTTCAGGCCGCCCTCGACCGGCAGGCCGGGGCAACCGGCGGTCTCTTCTGGATGCGGG
GCGCCCGTTTTTCAGGCTACGAGGCCCAGGCTTCAGGCGCTCTGGTGCATCTTGAGGGCGGCGAAAGCTTCCGCTGCGGG
CTGATCGCCGCGTGTGACGGCATTCATTCGGCAGTCCGGCAAGCGGCCGGGATCGGAACCGAGGGGCGCAGCTATGGAAA
ATCGGTCTTTGCCGCCGATGTGAAGCTTGCCCGGCCCCATGGCGGGATTGCCCGGCAATTGTTCACGCCCGAAGGCCCGT
TTGCGACCCTTCCCCTGCCCGATAACCGGGCAAACCTTGCCTGGTATATGAAGACGGGCGCGGCCGAGACACTGGCGAAG
CTCTCCAAAGAGCAGATCGAGGCGGAGCTGAACGCGCGGTTCTCAGAGTTTGCCGGGCCGATGACGCTGGACGGCCCTCC
CCTCTCCTATCCGCTGGTGATGCAGATCGCGCGCGATATGACCGGCCCGCGCGTGGCGCTGCTGGGCGACGCTGCCCGGC
GGATCAATCCCCTGGCCGGTCAGGGCCTGAACCTTGGCTTCAAGGATGCAGGCGCGCTTTATGATGTGATCCTGGAGGCG
CGTGAGGTTGGGCTGGACCCCGGCAGCGAGACTGTTCTGGCGCGTTACCGCGAGGGGCGCCGGTTTGATTCTGCCTCAAC
GGCGCTGTTCATGGACGCGGTGGACCGGGCGTTCTCGAATGACAATCTGCTGCTGAAGCCCTTGCGGTCGCTCGCCCTGA
CGGCCGCCAACAGGATTGGCCCGATCCGGCAGGCCCTCGCCCGCCAGGCCAGCGCCGATCAGGATCACCTGCCGAGTCTG
ATGCGTTAA

Upstream 100 bases:

>100_bases
GCACAGATGCGGCCAATCGCCCAAACGGGCGCCATCGCTAGACCCGCAGGCCTCTCGGAGCTACCTGAATTACATGGCAG
AAACCTCAAATCCCGCTCTT

Downstream 100 bases:

>100_bases
TCGTTGATCGTCAGTGCGCGGAGAATATTCCAGGTGTAGAGACCGCTGGAATGGCCGTCTGAGAAATGCAGGCGCAGCGC
GTAGCGCCCGATGGGCTCTG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEADLAVIGAGPVGTSLAVLAAKAGLRTVLVDMRPENAGPAADTRSFAIVRGSWRLLGAAGVHSDLAGLTEPLNGLEAMD
GGTHWFGAPWAAFSATDLPGDDPQPLGELVPAGALQAALDRQAGATGGLFWMRGARFSGYEAQASGALVHLEGGESFRCG
LIAACDGIHSAVRQAAGIGTEGRSYGKSVFAADVKLARPHGGIARQLFTPEGPFATLPLPDNRANLAWYMKTGAAETLAK
LSKEQIEAELNARFSEFAGPMTLDGPPLSYPLVMQIARDMTGPRVALLGDAARRINPLAGQGLNLGFKDAGALYDVILEA
REVGLDPGSETVLARYREGRRFDSASTALFMDAVDRAFSNDNLLLKPLRSLALTAANRIGPIRQALARQASADQDHLPSL
MR

Sequences:

>Translated_402_residues
MEADLAVIGAGPVGTSLAVLAAKAGLRTVLVDMRPENAGPAADTRSFAIVRGSWRLLGAAGVHSDLAGLTEPLNGLEAMD
GGTHWFGAPWAAFSATDLPGDDPQPLGELVPAGALQAALDRQAGATGGLFWMRGARFSGYEAQASGALVHLEGGESFRCG
LIAACDGIHSAVRQAAGIGTEGRSYGKSVFAADVKLARPHGGIARQLFTPEGPFATLPLPDNRANLAWYMKTGAAETLAK
LSKEQIEAELNARFSEFAGPMTLDGPPLSYPLVMQIARDMTGPRVALLGDAARRINPLAGQGLNLGFKDAGALYDVILEA
REVGLDPGSETVLARYREGRRFDSASTALFMDAVDRAFSNDNLLLKPLRSLALTAANRIGPIRQALARQASADQDHLPSL
MR
>Mature_402_residues
MEADLAVIGAGPVGTSLAVLAAKAGLRTVLVDMRPENAGPAADTRSFAIVRGSWRLLGAAGVHSDLAGLTEPLNGLEAMD
GGTHWFGAPWAAFSATDLPGDDPQPLGELVPAGALQAALDRQAGATGGLFWMRGARFSGYEAQASGALVHLEGGESFRCG
LIAACDGIHSAVRQAAGIGTEGRSYGKSVFAADVKLARPHGGIARQLFTPEGPFATLPLPDNRANLAWYMKTGAAETLAK
LSKEQIEAELNARFSEFAGPMTLDGPPLSYPLVMQIARDMTGPRVALLGDAARRINPLAGQGLNLGFKDAGALYDVILEA
REVGLDPGSETVLARYREGRRFDSASTALFMDAVDRAFSNDNLLLKPLRSLALTAANRIGPIRQALARQASADQDHLPSL
MR

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=334, Percent_Identity=28.7425149700599, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI32967307, Length=351, Percent_Identity=28.4900284900285, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786883, Length=392, Percent_Identity=33.9285714285714, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1789273, Length=409, Percent_Identity=28.8508557457213, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=283, Percent_Identity=30.7420494699647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920760, Length=284, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42147; Mature: 42147

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADLAVIGAGPVGTSLAVLAAKAGLRTVLVDMRPENAGPAADTRSFAIVRGSWRLLGAA
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEECC
GVHSDLAGLTEPLNGLEAMDGGTHWFGAPWAAFSATDLPGDDPQPLGELVPAGALQAALD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHCCCHHHHHHHH
RQAGATGGLFWMRGARFSGYEAQASGALVHLEGGESFRCGLIAACDGIHSAVRQAAGIGT
HHCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
EGRSYGKSVFAADVKLARPHGGIARQLFTPEGPFATLPLPDNRANLAWYMKTGAAETLAK
CCHHHCCCEEEECEEEECCCCCHHHHHCCCCCCEEECCCCCCCCCEEEEEECCHHHHHHH
LSKEQIEAELNARFSEFAGPMTLDGPPLSYPLVMQIARDMTGPRVALLGDAARRINPLAG
HHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCC
QGLNLGFKDAGALYDVILEAREVGLDPGSETVLARYREGRRFDSASTALFMDAVDRAFSN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DNLLLKPLRSLALTAANRIGPIRQALARQASADQDHLPSLMR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCHHHCC
>Mature Secondary Structure
MEADLAVIGAGPVGTSLAVLAAKAGLRTVLVDMRPENAGPAADTRSFAIVRGSWRLLGAA
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEECC
GVHSDLAGLTEPLNGLEAMDGGTHWFGAPWAAFSATDLPGDDPQPLGELVPAGALQAALD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHCCCHHHHHHHH
RQAGATGGLFWMRGARFSGYEAQASGALVHLEGGESFRCGLIAACDGIHSAVRQAAGIGT
HHCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
EGRSYGKSVFAADVKLARPHGGIARQLFTPEGPFATLPLPDNRANLAWYMKTGAAETLAK
CCHHHCCCEEEECEEEECCCCCHHHHHCCCCCCEEECCCCCCCCCEEEEEECCHHHHHHH
LSKEQIEAELNARFSEFAGPMTLDGPPLSYPLVMQIARDMTGPRVALLGDAARRINPLAG
HHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCC
QGLNLGFKDAGALYDVILEAREVGLDPGSETVLARYREGRRFDSASTALFMDAVDRAFSN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DNLLLKPLRSLALTAANRIGPIRQALARQASADQDHLPSLMR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]