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The map label for this gene is nylA [H]

Identifier: 114798308

GI number: 114798308

Start: 1354295

End: 1355743

Strand: Reverse

Name: nylA [H]

Synonym: HNE_1322

Alternate gene names: 114798308

Gene position: 1355743-1354295 (Counterclockwise)

Preceding gene: 114799385

Following gene: 114799005

Centisome position: 36.59

GC content: 66.53

Gene sequence:

>1449_bases
ATGGCGCGCGATTCAGAATTGGACGGCCTGGGCCTTGCCGAGCTTGTTCGCACACGGCAGGCATCGCCGGGTGAACTCCT
CGAAGCCGCCGTCAGCCGCGCCGAATCTGTCCAGCCTCAGATCAACTGTTTCTCGGCGCTTTATCCTGATCTTGCGCGCG
AGCAGCTTAAAGCAGGCGGCATCGACGGCCCCTATGCCGGGGTGCCGTTCGTGGTGAAGGATCTGGGCGTTGAGGTGAAA
GGCGCGCCCGTTACTTCGGGCAGCCGCGCCTTCAAGGGCTATGTGGCAAGCCGCGATTCCACCCTGGTGGAGCGGTTCCG
CAAGGCAGGCCTCGTTTTCTTTGGCGCAACCACCTCGCCGGAATTCGGCCTCACCCTGACAACAGAATCGACCCTCTACG
GACAATCCCGAAACCCCTGGGACACCACCCGCATCACAGGCGGCTCCTCTGGCGGCGCCGCAGCGGCGGTCGCCTCCGGC
GTTCTGCCAGTGGCACATGCCAGCGATGGCGGTGGATCGATCCGCATTCCTGCGGCGTGCTGCGGCCTGTTTGGCCTCAA
GGCATCGCGCGGGCGCATGCCCATGGGCCCGGCCAAAACCGAAGGCTGGAACGGTCTCTCAACGGTCGGCGTGGTCAGCC
GCACGGTGCGCGACACGGCCGCGATGCTGGACCTGACCCATGGCCCGGAAACCGGCAGCCGCTACGCCGCGCCTGCTCCG
GCCCGCGCCTATCTCTCCGAGCTGGACCGCGACCCGCGCCCGCTGCGCATCGCGCTCTGGCCGGTCGCGCCTAATGGTAC
AAAGCCGGACGCCGACGCGGCTGAGGGGATTGCAAACACGGCAAAACTCCTCGAAAGCCTCGGCCACACAGTCGTGGAAG
CTGCGCCATTGATTGAGGGTGAAGCGCTGGCCAAGGCAGGTTTGTTCACGATCTCCGCCAACATCGCCGCCCTGGTAGAA
GAGCGCGGCATCGCGCGCGGACTCCCGGTGGAGGATGACGAACTCGAACCCATCACCGCCTCCATGGTGCGCCTTGGACG
GACGGTTCCGATGGTGGAGCTGGCCAAGGCAAACAACGCCTTCATCACGGCGGCGATCATCTATGAGCACTTCCTGGATG
CCGGGCAGTTTGATCTCACATTGTCGCCCACGCTTCACCGCGCGCCAGACCCGCTCGGCACCATGGCGCTTACCGCAGAC
CCTGCGGCCATGGGCGCGGCGGTCGCAGGCTTTGCGCCCCATTGCGCGCTGTTCAACCAGACCGGCTGCCCCGCCATGAC
CGTGCCGCTGCACTGGACGAAGCCCACGCCCACCGCGCCCGCGGGCCTGCCTATTGGCATGATGTTCGGCGCGCGCTATG
GCCGCGAAGACCTGCTGCTGTCGCTGGCAGGACAGTTGGAGCGCGCCGCGCCATGGGCACACAGGAAACCGCCGGTCTGG
GCCGGCTGA

Upstream 100 bases:

>100_bases
TTCGGCCTTGCCAGCCCCCTCCCGTTTGCGCCGTTCAAACAATCCCTTACAAAGGAGGAAGCATATAAATCATGATAAAG
AAATCAGGGAGATAGACAGC

Downstream 100 bases:

>100_bases
ACGCAGAAAGTCCGCGCTGGCATAGTGGCGGCTGCCATTGCCGCGCTGTGCGCTTTGAGGTGCAACTGCCCGAAGCTTTC
GAGGTGGAAGACTGCAATTG

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MARDSELDGLGLAELVRTRQASPGELLEAAVSRAESVQPQINCFSALYPDLAREQLKAGGIDGPYAGVPFVVKDLGVEVK
GAPVTSGSRAFKGYVASRDSTLVERFRKAGLVFFGATTSPEFGLTLTTESTLYGQSRNPWDTTRITGGSSGGAAAAVASG
VLPVAHASDGGGSIRIPAACCGLFGLKASRGRMPMGPAKTEGWNGLSTVGVVSRTVRDTAAMLDLTHGPETGSRYAAPAP
ARAYLSELDRDPRPLRIALWPVAPNGTKPDADAAEGIANTAKLLESLGHTVVEAAPLIEGEALAKAGLFTISANIAALVE
ERGIARGLPVEDDELEPITASMVRLGRTVPMVELAKANNAFITAAIIYEHFLDAGQFDLTLSPTLHRAPDPLGTMALTAD
PAAMGAAVAGFAPHCALFNQTGCPAMTVPLHWTKPTPTAPAGLPIGMMFGARYGREDLLLSLAGQLERAAPWAHRKPPVW
AG

Sequences:

>Translated_482_residues
MARDSELDGLGLAELVRTRQASPGELLEAAVSRAESVQPQINCFSALYPDLAREQLKAGGIDGPYAGVPFVVKDLGVEVK
GAPVTSGSRAFKGYVASRDSTLVERFRKAGLVFFGATTSPEFGLTLTTESTLYGQSRNPWDTTRITGGSSGGAAAAVASG
VLPVAHASDGGGSIRIPAACCGLFGLKASRGRMPMGPAKTEGWNGLSTVGVVSRTVRDTAAMLDLTHGPETGSRYAAPAP
ARAYLSELDRDPRPLRIALWPVAPNGTKPDADAAEGIANTAKLLESLGHTVVEAAPLIEGEALAKAGLFTISANIAALVE
ERGIARGLPVEDDELEPITASMVRLGRTVPMVELAKANNAFITAAIIYEHFLDAGQFDLTLSPTLHRAPDPLGTMALTAD
PAAMGAAVAGFAPHCALFNQTGCPAMTVPLHWTKPTPTAPAGLPIGMMFGARYGREDLLLSLAGQLERAAPWAHRKPPVW
AG
>Mature_481_residues
ARDSELDGLGLAELVRTRQASPGELLEAAVSRAESVQPQINCFSALYPDLAREQLKAGGIDGPYAGVPFVVKDLGVEVKG
APVTSGSRAFKGYVASRDSTLVERFRKAGLVFFGATTSPEFGLTLTTESTLYGQSRNPWDTTRITGGSSGGAAAAVASGV
LPVAHASDGGGSIRIPAACCGLFGLKASRGRMPMGPAKTEGWNGLSTVGVVSRTVRDTAAMLDLTHGPETGSRYAAPAPA
RAYLSELDRDPRPLRIALWPVAPNGTKPDADAAEGIANTAKLLESLGHTVVEAAPLIEGEALAKAGLFTISANIAALVEE
RGIARGLPVEDDELEPITASMVRLGRTVPMVELAKANNAFITAAIIYEHFLDAGQFDLTLSPTLHRAPDPLGTMALTADP
AAMGAAVAGFAPHCALFNQTGCPAMTVPLHWTKPTPTAPAGLPIGMMFGARYGREDLLLSLAGQLERAAPWAHRKPPVWA
G

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=243, Percent_Identity=31.6872427983539, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI166795287, Length=301, Percent_Identity=29.5681063122924, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=25.1497005988024, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=239, Percent_Identity=31.3807531380753, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=307, Percent_Identity=29.9674267100977, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=309, Percent_Identity=29.126213592233, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=309, Percent_Identity=29.126213592233, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=130, Percent_Identity=37.6923076923077, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=293, Percent_Identity=27.6450511945392, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=213, Percent_Identity=30.5164319248826, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=128, Percent_Identity=39.0625, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=195, Percent_Identity=29.7435897435897, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=194, Percent_Identity=29.8969072164948, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550774, Length=259, Percent_Identity=31.6602316602317, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=259, Percent_Identity=31.6602316602317, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648437, Length=259, Percent_Identity=31.6602316602317, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=23.8955823293173, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24652985, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922090, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652981, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652983, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=27.0161290322581, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356731, Length=271, Percent_Identity=29.1512915129151, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=25.8252427184466, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 50174; Mature: 50043

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDSELDGLGLAELVRTRQASPGELLEAAVSRAESVQPQINCFSALYPDLAREQLKAGG
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
IDGPYAGVPFVVKDLGVEVKGAPVTSGSRAFKGYVASRDSTLVERFRKAGLVFFGATTSP
CCCCCCCCCHHHHHCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCC
EFGLTLTTESTLYGQSRNPWDTTRITGGSSGGAAAAVASGVLPVAHASDGGGSIRIPAAC
CCCEEEEECCEEECCCCCCCCCCEECCCCCCCHHHHHHHCCCEEEECCCCCCEEEHHHHH
CGLFGLKASRGRMPMGPAKTEGWNGLSTVGVVSRTVRDTAAMLDLTHGPETGSRYAAPAP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
ARAYLSELDRDPRPLRIALWPVAPNGTKPDADAAEGIANTAKLLESLGHTVVEAAPLIEG
HHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
EALAKAGLFTISANIAALVEERGIARGLPVEDDELEPITASMVRLGRTVPMVELAKANNA
HHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHEEECCCCC
FITAAIIYEHFLDAGQFDLTLSPTLHRAPDPLGTMALTADPAAMGAAVAGFAPHCALFNQ
EEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHCCC
TGCPAMTVPLHWTKPTPTAPAGLPIGMMFGARYGREDLLLSLAGQLERAAPWAHRKPPVW
CCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AG
CC
>Mature Secondary Structure 
ARDSELDGLGLAELVRTRQASPGELLEAAVSRAESVQPQINCFSALYPDLAREQLKAGG
CCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
IDGPYAGVPFVVKDLGVEVKGAPVTSGSRAFKGYVASRDSTLVERFRKAGLVFFGATTSP
CCCCCCCCCHHHHHCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCC
EFGLTLTTESTLYGQSRNPWDTTRITGGSSGGAAAAVASGVLPVAHASDGGGSIRIPAAC
CCCEEEEECCEEECCCCCCCCCCEECCCCCCCHHHHHHHCCCEEEECCCCCCEEEHHHHH
CGLFGLKASRGRMPMGPAKTEGWNGLSTVGVVSRTVRDTAAMLDLTHGPETGSRYAAPAP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
ARAYLSELDRDPRPLRIALWPVAPNGTKPDADAAEGIANTAKLLESLGHTVVEAAPLIEG
HHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
EALAKAGLFTISANIAALVEERGIARGLPVEDDELEPITASMVRLGRTVPMVELAKANNA
HHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHEEECCCCC
FITAAIIYEHFLDAGQFDLTLSPTLHRAPDPLGTMALTADPAAMGAAVAGFAPHCALFNQ
EEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHCCC
TGCPAMTVPLHWTKPTPTAPAGLPIGMMFGARYGREDLLLSLAGQLERAAPWAHRKPPVW
CCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]