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The map label for this gene is hisH [H]

Identifier: 114798251

GI number: 114798251

Start: 56936

End: 57568

Strand: Reverse

Name: hisH [H]

Synonym: HNE_0071

Alternate gene names: 114798251

Gene position: 57568-56936 (Counterclockwise)

Preceding gene: 114799970

Following gene: 114797166

Centisome position: 1.55

GC content: 67.93

Gene sequence:

>633_bases
ATGACGCGTGTCGCCCTCATTGATTACGGATCAGGCAACCTGCACTCCTGCGCGCGGGCGCTGGCCGCCGCGGCCGGAGA
CGCGGGCGCCGTTCACGTCACGTCCCGCCCCGAAGACATCCTCAATGCCGAACGCATCGTATTGCCCGGCGTCGGCCACT
TTGCCGACTGCGCAGGCGCGCTGCGGGCCATTCCGGGCATGGTCGAGGCGCTGGACGAGGCGGTGACACGCAAGGGGCGG
CCATTCCTGGGCATCTGCGTTGGCATGCAGCTGATGGCGGATATGGGCCTGGAAGACGGCGAGACGCGCGGCCTTGGCTG
GGTGCATGGCATTGTCGAGGCGCTGGAACCGGCGCCGGGCCTGCCGGTGCCCCATGTGGGCTGGAACGAGCTGATGATCC
CGGCGCCCCACCCGGTGCTGGAGGGCCTCGGCGAGACGCCCCACGCCTATTTCACCCATTCCTACGTGTTCCGCCCCGAA
GACGAGGCAAAGGTTGCCGCCTGGACCGAGTATGGCCCGCCCTTCGCCGCCGCCATCGCGCGCGACAACCTCATCGGCAC
GCAATTCCATCCCGAAAAGAGCCAGCGCCTCGGCCTGACGCTGCTCGCCAACTTCCTGAAATGGCACCCATGA

Upstream 100 bases:

>100_bases
TGATGGCGCGCCATATGCCGCAATCTGCGATTTCCGCCAAGCGACATTTCTGGGGGCGGCTTAATTGCCATGCTCCCCTG
CCCCCGCTAAGAGCGCGGCC

Downstream 100 bases:

>100_bases
CCTTCACCCTCTATCCCGCCATTGACCTGAAAGACGGCGCCTGCGTGCGCCTCCTGCGCGGGGAGATGGACGCGGCCACC
GTGTTCAACACGGACCCCGC

Product: imidazoleglycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTRVALIDYGSGNLHSCARALAAAAGDAGAVHVTSRPEDILNAERIVLPGVGHFADCAGALRAIPGMVEALDEAVTRKGR
PFLGICVGMQLMADMGLEDGETRGLGWVHGIVEALEPAPGLPVPHVGWNELMIPAPHPVLEGLGETPHAYFTHSYVFRPE
DEAKVAAWTEYGPPFAAAIARDNLIGTQFHPEKSQRLGLTLLANFLKWHP

Sequences:

>Translated_210_residues
MTRVALIDYGSGNLHSCARALAAAAGDAGAVHVTSRPEDILNAERIVLPGVGHFADCAGALRAIPGMVEALDEAVTRKGR
PFLGICVGMQLMADMGLEDGETRGLGWVHGIVEALEPAPGLPVPHVGWNELMIPAPHPVLEGLGETPHAYFTHSYVFRPE
DEAKVAAWTEYGPPFAAAIARDNLIGTQFHPEKSQRLGLTLLANFLKWHP
>Mature_209_residues
TRVALIDYGSGNLHSCARALAAAAGDAGAVHVTSRPEDILNAERIVLPGVGHFADCAGALRAIPGMVEALDEAVTRKGRP
FLGICVGMQLMADMGLEDGETRGLGWVHGIVEALEPAPGLPVPHVGWNELMIPAPHPVLEGLGETPHAYFTHSYVFRPED
EAKVAAWTEYGPPFAAAIARDNLIGTQFHPEKSQRLGLTLLANFLKWHP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=34.4339622641509, Blast_Score=110, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=220, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22437; Mature: 22306

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVALIDYGSGNLHSCARALAAAAGDAGAVHVTSRPEDILNAERIVLPGVGHFADCAGA
CCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHH
LRAIPGMVEALDEAVTRKGRPFLGICVGMQLMADMGLEDGETRGLGWVHGIVEALEPAPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
LPVPHVGWNELMIPAPHPVLEGLGETPHAYFTHSYVFRPEDEAKVAAWTEYGPPFAAAIA
CCCCCCCCCCEECCCCCHHHHCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCHHHHHH
RDNLIGTQFHPEKSQRLGLTLLANFLKWHP
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRVALIDYGSGNLHSCARALAAAAGDAGAVHVTSRPEDILNAERIVLPGVGHFADCAGA
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHH
LRAIPGMVEALDEAVTRKGRPFLGICVGMQLMADMGLEDGETRGLGWVHGIVEALEPAPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
LPVPHVGWNELMIPAPHPVLEGLGETPHAYFTHSYVFRPEDEAKVAAWTEYGPPFAAAIA
CCCCCCCCCCEECCCCCHHHHCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCHHHHHH
RDNLIGTQFHPEKSQRLGLTLLANFLKWHP
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA