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The map label for this gene is mclA [H]

Identifier: 114798208

GI number: 114798208

Start: 62935

End: 63990

Strand: Reverse

Name: mclA [H]

Synonym: HNE_0078

Alternate gene names: 114798208

Gene position: 63990-62935 (Counterclockwise)

Preceding gene: 114797932

Following gene: 114797338

Centisome position: 1.73

GC content: 63.92

Gene sequence:

>1056_bases
ATGAGCCAGAAAACGCCGCGCAGTTTCTTCAAGCCTCTGTCGATCGGCGCGCCGGAGCCCTATCGCGAACTTCCGGTCAA
ACTTGAGCGCATGATCCATTTCGTGCCGCCCCATCTGGATAAGGTACGCGCCAAGGTGCCGGACATGGCGCCTACCGTGG
ATGTCATCCTCGCAAACCTCGAAGACGCCATTCCCGCCGACGCCAAGGACGCCGCCCGCGCCGGGGCCATCGAAATGGCC
AACATGTACGACTGGCAGGGCAAGGGCACGGGCTTCTGGAGCCGCGTGAACTGTCTCAACTCTCCCTGGTTTCAAGACGA
TGTGTCTGAGCTGGTCCTTAAGGCCGGCCACCAGCTGGACGTGATCATGCTGCCCAAGGTCGAGGGGCCGTGGGATATTC
ACTTTGCCGACCAGTATGTCGCCCAGCTCGAGGCGAAAGCTGGTCTGACCCGCCCGATCCTCTTCCATGCCATCCTTGAG
ACGGCCGAGGGCGTTGCCCGCGTCGAGGAGATCGCGCTTGCCTCCCCCCGCATGCAGGGCATCAGCCTTGGCCCGGCAGA
CCTTGCCGCCAGCCGCAAGATGAAGACCACCCGCGTCGGCGGGGGCCATCCCTTCTACCGCGTGATCGAAGACCCCAAGG
AAGATGGCAGCGCGCGCCTGAGCGCCCAGCAGGACCCTTGGCATTACACGATTGCCCGCCTGGTGGACGCCTGCCGCATG
GCCGGGATCAACGCCTTCTACGGCCCGTTCGGCGACATCGGCGATCTGGAAGCCTGCGAGCAGCAGTTCCGCAACGCCTT
CCTTCTGGGCTGCGCGGGCACCTGGAGCCTCCACCCCAGCCAGATAGCGGTGGCCAAGCGCGTCTTCAGCCCCGATCCGG
AGGAAGTGAAGTTTGCCCGCAAGATCCTCGCCGCCATGCCGGATGGCTCAGGCGTGGCGATGATCGACGGCAAGATGCAG
GACGACGCCACCTGGAAACAGGCAAAGGTGATCTCTGATCTGGCAGACCTTGTGGCCTCGAAAGATCCCGATCTGGCGGC
CGCTTATGCGGGGTGA

Upstream 100 bases:

>100_bases
GGCATAAACGCACAAAGCCGGTGAGCGTTCCGTCACACGCCAACTGTTTGACGCGCTGCAGCACGGGCACTAATCCTTGC
GCCACAAAAGGGAGCTTCCA

Downstream 100 bases:

>100_bases
TTTGGCTCTCGCCAAACAGCGCAGAGACACGTAGCTTCAGCCCTGACAAACGCGGCCCCTCAAGGGTCAAAGGCGCACAG
GAGCATGCCATGCTGAGGAC

Product: malyl-CoA lyase

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSQKTPRSFFKPLSIGAPEPYRELPVKLERMIHFVPPHLDKVRAKVPDMAPTVDVILANLEDAIPADAKDAARAGAIEMA
NMYDWQGKGTGFWSRVNCLNSPWFQDDVSELVLKAGHQLDVIMLPKVEGPWDIHFADQYVAQLEAKAGLTRPILFHAILE
TAEGVARVEEIALASPRMQGISLGPADLAASRKMKTTRVGGGHPFYRVIEDPKEDGSARLSAQQDPWHYTIARLVDACRM
AGINAFYGPFGDIGDLEACEQQFRNAFLLGCAGTWSLHPSQIAVAKRVFSPDPEEVKFARKILAAMPDGSGVAMIDGKMQ
DDATWKQAKVISDLADLVASKDPDLAAAYAG

Sequences:

>Translated_351_residues
MSQKTPRSFFKPLSIGAPEPYRELPVKLERMIHFVPPHLDKVRAKVPDMAPTVDVILANLEDAIPADAKDAARAGAIEMA
NMYDWQGKGTGFWSRVNCLNSPWFQDDVSELVLKAGHQLDVIMLPKVEGPWDIHFADQYVAQLEAKAGLTRPILFHAILE
TAEGVARVEEIALASPRMQGISLGPADLAASRKMKTTRVGGGHPFYRVIEDPKEDGSARLSAQQDPWHYTIARLVDACRM
AGINAFYGPFGDIGDLEACEQQFRNAFLLGCAGTWSLHPSQIAVAKRVFSPDPEEVKFARKILAAMPDGSGVAMIDGKMQ
DDATWKQAKVISDLADLVASKDPDLAAAYAG
>Mature_350_residues
SQKTPRSFFKPLSIGAPEPYRELPVKLERMIHFVPPHLDKVRAKVPDMAPTVDVILANLEDAIPADAKDAARAGAIEMAN
MYDWQGKGTGFWSRVNCLNSPWFQDDVSELVLKAGHQLDVIMLPKVEGPWDIHFADQYVAQLEAKAGLTRPILFHAILET
AEGVARVEEIALASPRMQGISLGPADLAASRKMKTTRVGGGHPFYRVIEDPKEDGSARLSAQQDPWHYTIARLVDACRMA
GINAFYGPFGDIGDLEACEQQFRNAFLLGCAGTWSLHPSQIAVAKRVFSPDPEEVKFARKILAAMPDGSGVAMIDGKMQD
DATWKQAKVISDLADLVASKDPDLAAAYAG

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=321, Percent_Identity=28.0373831775701, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI87081764, Length=289, Percent_Identity=26.9896193771626, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 38364; Mature: 38233

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKTPRSFFKPLSIGAPEPYRELPVKLERMIHFVPPHLDKVRAKVPDMAPTVDVILANL
CCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
EDAIPADAKDAARAGAIEMANMYDWQGKGTGFWSRVNCLNSPWFQDDVSELVLKAGHQLD
HHCCCCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
VIMLPKVEGPWDIHFADQYVAQLEAKAGLTRPILFHAILETAEGVARVEEIALASPRMQG
EEEECCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ISLGPADLAASRKMKTTRVGGGHPFYRVIEDPKEDGSARLSAQQDPWHYTIARLVDACRM
CCCCHHHHHHHCCCCEEECCCCCCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHH
AGINAFYGPFGDIGDLEACEQQFRNAFLLGCAGTWSLHPSQIAVAKRVFSPDPEEVKFAR
CCCCHHCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
KILAAMPDGSGVAMIDGKMQDDATWKQAKVISDLADLVASKDPDLAAAYAG
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQKTPRSFFKPLSIGAPEPYRELPVKLERMIHFVPPHLDKVRAKVPDMAPTVDVILANL
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
EDAIPADAKDAARAGAIEMANMYDWQGKGTGFWSRVNCLNSPWFQDDVSELVLKAGHQLD
HHCCCCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
VIMLPKVEGPWDIHFADQYVAQLEAKAGLTRPILFHAILETAEGVARVEEIALASPRMQG
EEEECCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ISLGPADLAASRKMKTTRVGGGHPFYRVIEDPKEDGSARLSAQQDPWHYTIARLVDACRM
CCCCHHHHHHHCCCCEEECCCCCCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHH
AGINAFYGPFGDIGDLEACEQQFRNAFLLGCAGTWSLHPSQIAVAKRVFSPDPEEVKFAR
CCCCHHCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
KILAAMPDGSGVAMIDGKMQDDATWKQAKVISDLADLVASKDPDLAAAYAG
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA