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The map label for this gene is ldh2 [H]

Identifier: 114798110

GI number: 114798110

Start: 2897114

End: 2898169

Strand: Direct

Name: ldh2 [H]

Synonym: HNE_2735

Alternate gene names: 114798110

Gene position: 2897114-2898169 (Clockwise)

Preceding gene: 114800124

Following gene: 114799771

Centisome position: 78.19

GC content: 62.78

Gene sequence:

>1056_bases
ATGTTTGATCACCCCTCTTTTGACGCCCATGAGGGCGTTCATGCCTTTCACGATGCGCAAAGCGGGCTGAGAGCCATTAT
TGCCGTTCATTCCACGGCGCTGGGCCCGGCGGCCGGCGGCTGCCGGATGTGGAATTATGCCAGCGGCGACGCGATGCTGA
CCGACGCGCTGCGCCTGTCGCAGGGGATGAGCTTCAAGAACGCCATGGCGGATCTGCCGCTGGGCGGGGGCAAATCGGTG
ATCTGGGGCGACCCGCGCAAGGACAAGTCCGAGGCGCTTTTCCGCGCCTTTGGCCGCGCCGTCGAAAGCCTTCAGGGCCA
GTATTACACGGCCGAAGACGTTGGCATCGACACATCCGACATGGCGCTCGTGCGCAAGGAAACGCGCTATGTGGCCGGCC
TGGATGAAGGCGCCGCCGCCAGTGGCGATCCCTCGCCGATCACCGCCATGGGCGTTTATCTGGGTATCAAGGAAGTGGCC
AAGCGACTTTACGGATCAGATGACCTCTCCGGCCGGATCGTGGCTGTGCAGGGGGTCGGCTCTGTCGGCGCGCATGTGTG
CGAGCATCTGGCCAAGGATGGCGCAAAGCTCATCATTTCCGACATCGATCAGGAGGCGCTGAAGGATGTGTCCACCCAGA
CCGGCGCCACAGTCGTTCAGCCTGATGACATCTATGATGTGGAAGCCGACATCTTCTCGCCCTGCGCGCTGGGCGCGGTC
ATCAACGAGAAGACACTGGAGCGGCTTCAGGTCAAAGGCGTGGCGGGCGCAGCGAACAACCAGCTGGTCGTGCCGGAAAT
GGGCGAACTGTTGCGCCGCAAAGGCATTCTCTATGCGCCAGACTATGTCATCAATGGCGGCGGGATCATCAATGTGGCCG
CCGAAATCTCCGGCAACTATTCGCGCGAATGGGTTGATGGGAAGCTCGCCCGCCTGATCGAGACGCTGGGCGAGGTGCTC
GACGAGGCCCTCTCCACCAACCAGCCCGCGAACGCGGTGGCCGACCGGATCGCCCGGAAACGTATTGCGGACGCCAAAGG
CCTCGCGCCTAAGTAG

Upstream 100 bases:

>100_bases
AATATGATACGGCCTGCGCTGGCGGCGCCAGTTGACGCTTCGGCGCCCACCCGGCTACAGCGCTGACAACCGTTTCAGTT
GCAACCATTTCCGGCACATC

Downstream 100 bases:

>100_bases
ACTTCCCTAGTCCGGAACCATGCCTCTGCTGTTACGTTCATGTTATAACTCCCGGCTGGGAGTGGTTGAACAGCAGGAGA
GAGCCCATGTCAGTCGTTGC

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MFDHPSFDAHEGVHAFHDAQSGLRAIIAVHSTALGPAAGGCRMWNYASGDAMLTDALRLSQGMSFKNAMADLPLGGGKSV
IWGDPRKDKSEALFRAFGRAVESLQGQYYTAEDVGIDTSDMALVRKETRYVAGLDEGAAASGDPSPITAMGVYLGIKEVA
KRLYGSDDLSGRIVAVQGVGSVGAHVCEHLAKDGAKLIISDIDQEALKDVSTQTGATVVQPDDIYDVEADIFSPCALGAV
INEKTLERLQVKGVAGAANNQLVVPEMGELLRRKGILYAPDYVINGGGIINVAAEISGNYSREWVDGKLARLIETLGEVL
DEALSTNQPANAVADRIARKRIADAKGLAPK

Sequences:

>Translated_351_residues
MFDHPSFDAHEGVHAFHDAQSGLRAIIAVHSTALGPAAGGCRMWNYASGDAMLTDALRLSQGMSFKNAMADLPLGGGKSV
IWGDPRKDKSEALFRAFGRAVESLQGQYYTAEDVGIDTSDMALVRKETRYVAGLDEGAAASGDPSPITAMGVYLGIKEVA
KRLYGSDDLSGRIVAVQGVGSVGAHVCEHLAKDGAKLIISDIDQEALKDVSTQTGATVVQPDDIYDVEADIFSPCALGAV
INEKTLERLQVKGVAGAANNQLVVPEMGELLRRKGILYAPDYVINGGGIINVAAEISGNYSREWVDGKLARLIETLGEVL
DEALSTNQPANAVADRIARKRIADAKGLAPK
>Mature_351_residues
MFDHPSFDAHEGVHAFHDAQSGLRAIIAVHSTALGPAAGGCRMWNYASGDAMLTDALRLSQGMSFKNAMADLPLGGGKSV
IWGDPRKDKSEALFRAFGRAVESLQGQYYTAEDVGIDTSDMALVRKETRYVAGLDEGAAASGDPSPITAMGVYLGIKEVA
KRLYGSDDLSGRIVAVQGVGSVGAHVCEHLAKDGAKLIISDIDQEALKDVSTQTGATVVQPDDIYDVEADIFSPCALGAV
INEKTLERLQVKGVAGAANNQLVVPEMGELLRRKGILYAPDYVINGGGIINVAAEISGNYSREWVDGKLARLIETLGEVL
DEALSTNQPANAVADRIARKRIADAKGLAPK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids. Catalyzes the reversible deamination of these branched-chain L-amino acids to their oxo analogs [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=304, Percent_Identity=25.3289473684211, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4885281, Length=304, Percent_Identity=24.3421052631579, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17544676, Length=311, Percent_Identity=26.0450160771704, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549226, Length=305, Percent_Identity=26.8852459016393, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45553475, Length=305, Percent_Identity=26.8852459016393, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24649460, Length=270, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 37108; Mature: 37108

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDHPSFDAHEGVHAFHDAQSGLRAIIAVHSTALGPAAGGCRMWNYASGDAMLTDALRLS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
QGMSFKNAMADLPLGGGKSVIWGDPRKDKSEALFRAFGRAVESLQGQYYTAEDVGIDTSD
HCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCHH
MALVRKETRYVAGLDEGAAASGDPSPITAMGVYLGIKEVAKRLYGSDDLSGRIVAVQGVG
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
SVGAHVCEHLAKDGAKLIISDIDQEALKDVSTQTGATVVQPDDIYDVEADIFSPCALGAV
HHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHCHHHHHHH
INEKTLERLQVKGVAGAANNQLVVPEMGELLRRKGILYAPDYVINGGGIINVAAEISGNY
HHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCEECCCEEECCCCEEEEEEECCCCC
SREWVDGKLARLIETLGEVLDEALSTNQPANAVADRIARKRIADAKGLAPK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFDHPSFDAHEGVHAFHDAQSGLRAIIAVHSTALGPAAGGCRMWNYASGDAMLTDALRLS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
QGMSFKNAMADLPLGGGKSVIWGDPRKDKSEALFRAFGRAVESLQGQYYTAEDVGIDTSD
HCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCHH
MALVRKETRYVAGLDEGAAASGDPSPITAMGVYLGIKEVAKRLYGSDDLSGRIVAVQGVG
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
SVGAHVCEHLAKDGAKLIISDIDQEALKDVSTQTGATVVQPDDIYDVEADIFSPCALGAV
HHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHCHHHHHHH
INEKTLERLQVKGVAGAANNQLVVPEMGELLRRKGILYAPDYVINGGGIINVAAEISGNY
HHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCEECCCEEECCCCEEEEEEECCCCC
SREWVDGKLARLIETLGEVLDEALSTNQPANAVADRIARKRIADAKGLAPK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8020469 [H]