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The map label for this gene is gspE [H]

Identifier: 114798020

GI number: 114798020

Start: 1932754

End: 1934244

Strand: Direct

Name: gspE [H]

Synonym: HNE_1866

Alternate gene names: 114798020

Gene position: 1932754-1934244 (Clockwise)

Preceding gene: 114797223

Following gene: 114798264

Centisome position: 52.17

GC content: 61.03

Gene sequence:

>1491_bases
ATGGCGCGCGCTCAGGTCACCTACTCATTTGCCCGCGACAAGGGGATTGTTGTTATCCCCGGCCGGTCGGATTTGACGTT
TGCCGTTCGCGAGGGCGCAGACCCGGCCGCGCTGCTTGAGGCGCGCCGCGTCACCGGACTCCCGGTGCATCTGGAAGCCA
TGAATTCTGCCCAGTACGAGCGCCAGTTGTCGGAGAGCTTCGGTCAAGGGCGGCTTGACGGCAGCGCTGCCGAAGCCGCC
GCCGGAACGGGCGGCGACCTGGAAAGCCTGTTTGACGGCCTTCCGCAAACGGAAGACCTTTTGGCCGGTGACACCGAAGC
CCCCATCATCACACTTATCAACGGCCTGCTTCAGGACGCCATCCGTCGCCGGGCATCTGATATTCATATTGATCCATTTG
AAGACAAAATGTCTGTCCGTTACCGGATTGATGGCGAGCTACAGGAAGTCTTCACGGCGTCCCGCCGCGTTGCCGCCCCG
CTGGTTTCACGCATCAAGGTGATGAGCCGCCTTGATATTGCAGAGAAACGCATGCCCCAGGACGGCCGCATCTCGTTGAC
GCTCGGTGGTCGCGCTCTGGATGTTCGTGTCGCGACCCTTCCAACACGCTACGGCGAACGGGTTGCCTTGCGTATACTCG
ATACCAAACAGGCATTGCTTGGTCTCGAAACCCTCGGCATGGACGAAAACACGGAGAAGCGTTTTCGCACGGCATTGAGC
GAGCCCAACGGCGTGATCCTGGTAACCGGCCCCACGGGCTCGGGCAAAACCACCACGCTTTATTCCGCCCTTGCCCAGCT
CAATACCGGTCGCGTCAATATCATGACGCTGGAAGACCCGGTAGAATACGGCCTCGACGGGATCAGCCAGACCCCGATGG
ATCACAAGGTCGGCCTCACCTTTGCCGCCACACTGCGCTCGATCCTCCGGAATGATCCCAACATCGTCATGGTCGGCGAA
ATTCGCGATGCGGAGACCGCCGAAGTTGCGCTTGAGTTTGCGCTGACCGGACGCCTCGCCCTGTCCACCATCCACACGAA
CAGCTCGGCAGGGGCGATCACGCGTCTGCGCGACATGGGCGTGGAAAGTTTTCTGCTTGCCTCCACGATGCGTGCAATCC
TGGCCCAGCGGCTGGTGCGCCGCCTGTGCGTTCATTGTAAGGAAGCTTACGCCCCACCCCCAGCTCTGTTGGAGCGGCTG
GGCCTTGAAGACCGGGCAGACCTGACCCTTTACCGGCCGGTCGGCTGCGCTTCGTGCGGGCAGACGGGTTATGAAGGCCG
CCTCGGTGTTTATGAACTTCTGCTTGTGGATACCGCGATCCGCCGCATGATCCATGAGGGGGCAACCGAAGAGCAGATTG
AGCGCTCAGCCTTCGCCAAATCTGATATGCTCTTCCAGAATGGACTGCGGCATGTGTTGAGCGGCGACTCAAGTGCTGAG
GAGTTGCTGCACGTCTGCCGCCGGGAGGCGTGGTTCGGTGGCAGCGTTTGA

Upstream 100 bases:

>100_bases
GAGCAATCCCCGGCCGGCTCCATTGACCAGTACATACGCGAAGTTCTGGGCACCGGCGGCCCGGCCACGCAACTGGTCCA
GCCGCCGCAATAATCCTCTC

Downstream 100 bases:

>100_bases
ATACAAAGCCCTGACAGCAGATGGCCGGCGCACCAGCGGCGTCATCTCGGCTGACACCGCCCGCGCCGCCCGGAAGGAAC
TCCGGGATCGCCAGCTGACG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MARAQVTYSFARDKGIVVIPGRSDLTFAVREGADPAALLEARRVTGLPVHLEAMNSAQYERQLSESFGQGRLDGSAAEAA
AGTGGDLESLFDGLPQTEDLLAGDTEAPIITLINGLLQDAIRRRASDIHIDPFEDKMSVRYRIDGELQEVFTASRRVAAP
LVSRIKVMSRLDIAEKRMPQDGRISLTLGGRALDVRVATLPTRYGERVALRILDTKQALLGLETLGMDENTEKRFRTALS
EPNGVILVTGPTGSGKTTTLYSALAQLNTGRVNIMTLEDPVEYGLDGISQTPMDHKVGLTFAATLRSILRNDPNIVMVGE
IRDAETAEVALEFALTGRLALSTIHTNSSAGAITRLRDMGVESFLLASTMRAILAQRLVRRLCVHCKEAYAPPPALLERL
GLEDRADLTLYRPVGCASCGQTGYEGRLGVYELLLVDTAIRRMIHEGATEEQIERSAFAKSDMLFQNGLRHVLSGDSSAE
ELLHVCRREAWFGGSV

Sequences:

>Translated_496_residues
MARAQVTYSFARDKGIVVIPGRSDLTFAVREGADPAALLEARRVTGLPVHLEAMNSAQYERQLSESFGQGRLDGSAAEAA
AGTGGDLESLFDGLPQTEDLLAGDTEAPIITLINGLLQDAIRRRASDIHIDPFEDKMSVRYRIDGELQEVFTASRRVAAP
LVSRIKVMSRLDIAEKRMPQDGRISLTLGGRALDVRVATLPTRYGERVALRILDTKQALLGLETLGMDENTEKRFRTALS
EPNGVILVTGPTGSGKTTTLYSALAQLNTGRVNIMTLEDPVEYGLDGISQTPMDHKVGLTFAATLRSILRNDPNIVMVGE
IRDAETAEVALEFALTGRLALSTIHTNSSAGAITRLRDMGVESFLLASTMRAILAQRLVRRLCVHCKEAYAPPPALLERL
GLEDRADLTLYRPVGCASCGQTGYEGRLGVYELLLVDTAIRRMIHEGATEEQIERSAFAKSDMLFQNGLRHVLSGDSSAE
ELLHVCRREAWFGGSV
>Mature_495_residues
ARAQVTYSFARDKGIVVIPGRSDLTFAVREGADPAALLEARRVTGLPVHLEAMNSAQYERQLSESFGQGRLDGSAAEAAA
GTGGDLESLFDGLPQTEDLLAGDTEAPIITLINGLLQDAIRRRASDIHIDPFEDKMSVRYRIDGELQEVFTASRRVAAPL
VSRIKVMSRLDIAEKRMPQDGRISLTLGGRALDVRVATLPTRYGERVALRILDTKQALLGLETLGMDENTEKRFRTALSE
PNGVILVTGPTGSGKTTTLYSALAQLNTGRVNIMTLEDPVEYGLDGISQTPMDHKVGLTFAATLRSILRNDPNIVMVGEI
RDAETAEVALEFALTGRLALSTIHTNSSAGAITRLRDMGVESFLLASTMRAILAQRLVRRLCVHCKEAYAPPPALLERLG
LEDRADLTLYRPVGCASCGQTGYEGRLGVYELLLVDTAIRRMIHEGATEEQIERSAFAKSDMLFQNGLRHVLSGDSSAEE
LLHVCRREAWFGGSV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=448, Percent_Identity=48.6607142857143, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786296, Length=391, Percent_Identity=42.7109974424552, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI87082188, Length=273, Percent_Identity=34.4322344322344, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54019; Mature: 53888

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAQVTYSFARDKGIVVIPGRSDLTFAVREGADPAALLEARRVTGLPVHLEAMNSAQYE
CCCCEEEEEECCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHH
RQLSESFGQGRLDGSAAEAAAGTGGDLESLFDGLPQTEDLLAGDTEAPIITLINGLLQDA
HHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
IRRRASDIHIDPFEDKMSVRYRIDGELQEVFTASRRVAAPLVSRIKVMSRLDIAEKRMPQ
HHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRISLTLGGRALDVRVATLPTRYGERVALRILDTKQALLGLETLGMDENTEKRFRTALS
CCEEEEEECCEEEEEEEEECCCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHC
EPNGVILVTGPTGSGKTTTLYSALAQLNTGRVNIMTLEDPVEYGLDGISQTPMDHKVGLT
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCHH
FAATLRSILRNDPNIVMVGEIRDAETAEVALEFALTGRLALSTIHTNSSAGAITRLRDMG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCC
VESFLLASTMRAILAQRLVRRLCVHCKEAYAPPPALLERLGLEDRADLTLYRPVGCASCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCC
QTGYEGRLGVYELLLVDTAIRRMIHEGATEEQIERSAFAKSDMLFQNGLRHVLSGDSSAE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELLHVCRREAWFGGSV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARAQVTYSFARDKGIVVIPGRSDLTFAVREGADPAALLEARRVTGLPVHLEAMNSAQYE
CCCEEEEEECCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHH
RQLSESFGQGRLDGSAAEAAAGTGGDLESLFDGLPQTEDLLAGDTEAPIITLINGLLQDA
HHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
IRRRASDIHIDPFEDKMSVRYRIDGELQEVFTASRRVAAPLVSRIKVMSRLDIAEKRMPQ
HHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRISLTLGGRALDVRVATLPTRYGERVALRILDTKQALLGLETLGMDENTEKRFRTALS
CCEEEEEECCEEEEEEEEECCCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHC
EPNGVILVTGPTGSGKTTTLYSALAQLNTGRVNIMTLEDPVEYGLDGISQTPMDHKVGLT
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCHH
FAATLRSILRNDPNIVMVGEIRDAETAEVALEFALTGRLALSTIHTNSSAGAITRLRDMG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCC
VESFLLASTMRAILAQRLVRRLCVHCKEAYAPPPALLERLGLEDRADLTLYRPVGCASCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCC
QTGYEGRLGVYELLLVDTAIRRMIHEGATEEQIERSAFAKSDMLFQNGLRHVLSGDSSAE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELLHVCRREAWFGGSV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]