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The map label for this gene is gspF [H]

Identifier: 114798264

GI number: 114798264

Start: 1934231

End: 1935442

Strand: Direct

Name: gspF [H]

Synonym: HNE_1867

Alternate gene names: 114798264

Gene position: 1934231-1935442 (Clockwise)

Preceding gene: 114798020

Following gene: 114799042

Centisome position: 52.21

GC content: 63.78

Gene sequence:

>1212_bases
GTGGCAGCGTTTGAATACAAAGCCCTGACAGCAGATGGCCGGCGCACCAGCGGCGTCATCTCGGCTGACACCGCCCGCGC
CGCCCGGAAGGAACTCCGGGATCGCCAGCTGACGCCGGTCGCGCTCACGGAGGCGCGCGGCGAAAAAGCGCTGGGCTTCG
CCGGGGCCGGCCGCCTCAGCGAAAAGGACCGCACCCTGCTGACCCGCCAGCTCGCCGTGCTCGTCTCCTCCGGCATGACC
ATCGAACAGGCTCTCACTGCTGCCGCGGGCGACAGCGACGCTTCTCCCCAGCGCGGCCTTCTCCTGGGTGTACGCTCGCA
GGTTATGGAAGGCTCAACCCTCGCCGAAGCGATGCGCGCAGCGCCGCGCGCCTTCCCGCCCCTCTTCCGCGCAGTCGTTT
CCTCTGGTGAGTCGTCGGGCCGCCTTGGCCTCGTCCTGGATCAACTCGCCCACCATCTTGAGCGCAGCTACCGGCTCCGC
ACCCTGGTGCGCTCGGCTCTGATCTATCCGGCGATCCTTGGCATCATGGCCACGCTGATGGTCACCGCCCTCATGGTATG
GGTGGTGCCCAAGCTGGTAGAGCAGTTTGCGATGTTGGGCGCCGACGAGCTGCCGCCGCTGACCCGGTTTGTCATTGGAC
TGTCCGGATTTGTCCGCGATTGGGGGCTCTTTGTGCTCGTGGGGGGCGCGATTTGTCTCTATTTGTCCACGATTGTCCTG
AAAGCGCCCGCCTGGAAACTGCGCTGGGATGCCTTCGTCCTGCGGATTCCCTTCTTCGGAGACATGGCGCGAAAAGTCTC
CGCCGCGCGCTTCGCCCGCATCCATGCCACCATGTCCGGCGCCGGCGCGACAGTCTTGGAAAGCCTCTCTGGCGCCCGTA
ACGCAATGGGAAATATGGTGTTTCGCTCCGCGGCCGATCAAATCCTGGAGATCGTCCAGCGCGGCGGCGCACTCTCTTCG
GCAATGAAATCCACAGGGGTCTTCCCCCAGATGATGGTCCACATGGTCGCCAGCGGCGAAGCCGCCCGCAACGTCCCCGC
TATGATGAACCGCGCCGCCGATTTCCTCGAAGACGAATTTGAAACCGCCACCAATGTTGCCCTAGGACTCCTGGAACCCA
TGATCATTATTTTCCTGGGCGGGATCGTCGGCACAATCGTTCTATCGATCATGCTGCCCATCATGCAGCTCAACACTCTC
GCCCTCGGATAA

Upstream 100 bases:

>100_bases
AAATCTGATATGCTCTTCCAGAATGGACTGCGGCATGTGTTGAGCGGCGACTCAAGTGCTGAGGAGTTGCTGCACGTCTG
CCGCCGGGAGGCGTGGTTCG

Downstream 100 bases:

>100_bases
AGGAGCGTCTTCATGTCCCCCGAAACAGAAACAAAGACTGCAGAAGTCCGCCGCAGTGAGGCGGGTTTCTCCCTTGTCGA
ACTGATGGTGGTTGTTTTCA

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAAFEYKALTADGRRTSGVISADTARAARKELRDRQLTPVALTEARGEKALGFAGAGRLSEKDRTLLTRQLAVLVSSGMT
IEQALTAAAGDSDASPQRGLLLGVRSQVMEGSTLAEAMRAAPRAFPPLFRAVVSSGESSGRLGLVLDQLAHHLERSYRLR
TLVRSALIYPAILGIMATLMVTALMVWVVPKLVEQFAMLGADELPPLTRFVIGLSGFVRDWGLFVLVGGAICLYLSTIVL
KAPAWKLRWDAFVLRIPFFGDMARKVSAARFARIHATMSGAGATVLESLSGARNAMGNMVFRSAADQILEIVQRGGALSS
AMKSTGVFPQMMVHMVASGEAARNVPAMMNRAADFLEDEFETATNVALGLLEPMIIIFLGGIVGTIVLSIMLPIMQLNTL
ALG

Sequences:

>Translated_403_residues
MAAFEYKALTADGRRTSGVISADTARAARKELRDRQLTPVALTEARGEKALGFAGAGRLSEKDRTLLTRQLAVLVSSGMT
IEQALTAAAGDSDASPQRGLLLGVRSQVMEGSTLAEAMRAAPRAFPPLFRAVVSSGESSGRLGLVLDQLAHHLERSYRLR
TLVRSALIYPAILGIMATLMVTALMVWVVPKLVEQFAMLGADELPPLTRFVIGLSGFVRDWGLFVLVGGAICLYLSTIVL
KAPAWKLRWDAFVLRIPFFGDMARKVSAARFARIHATMSGAGATVLESLSGARNAMGNMVFRSAADQILEIVQRGGALSS
AMKSTGVFPQMMVHMVASGEAARNVPAMMNRAADFLEDEFETATNVALGLLEPMIIIFLGGIVGTIVLSIMLPIMQLNTL
ALG
>Mature_402_residues
AAFEYKALTADGRRTSGVISADTARAARKELRDRQLTPVALTEARGEKALGFAGAGRLSEKDRTLLTRQLAVLVSSGMTI
EQALTAAAGDSDASPQRGLLLGVRSQVMEGSTLAEAMRAAPRAFPPLFRAVVSSGESSGRLGLVLDQLAHHLERSYRLRT
LVRSALIYPAILGIMATLMVTALMVWVVPKLVEQFAMLGADELPPLTRFVIGLSGFVRDWGLFVLVGGAICLYLSTIVLK
APAWKLRWDAFVLRIPFFGDMARKVSAARFARIHATMSGAGATVLESLSGARNAMGNMVFRSAADQILEIVQRGGALSSA
MKSTGVFPQMMVHMVASGEAARNVPAMMNRAADFLEDEFETATNVALGLLEPMIIIFLGGIVGTIVLSIMLPIMQLNTLA
LG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=399, Percent_Identity=32.5814536340852, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786295, Length=394, Percent_Identity=25.8883248730964, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43106; Mature: 42975

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFEYKALTADGRRTSGVISADTARAARKELRDRQLTPVALTEARGEKALGFAGAGRLS
CCCCCCCEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
EKDRTLLTRQLAVLVSSGMTIEQALTAAAGDSDASPQRGLLLGVRSQVMEGSTLAEAMRA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHH
APRAFPPLFRAVVSSGESSGRLGLVLDQLAHHLERSYRLRTLVRSALIYPAILGIMATLM
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTALMVWVVPKLVEQFAMLGADELPPLTRFVIGLSGFVRDWGLFVLVGGAICLYLSTIVL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAPAWKLRWDAFVLRIPFFGDMARKVSAARFARIHATMSGAGATVLESLSGARNAMGNMV
HCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FRSAADQILEIVQRGGALSSAMKSTGVFPQMMVHMVASGEAARNVPAMMNRAADFLEDEF
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
ETATNVALGLLEPMIIIFLGGIVGTIVLSIMLPIMQLNTLALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAFEYKALTADGRRTSGVISADTARAARKELRDRQLTPVALTEARGEKALGFAGAGRLS
CCCCCCEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
EKDRTLLTRQLAVLVSSGMTIEQALTAAAGDSDASPQRGLLLGVRSQVMEGSTLAEAMRA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHH
APRAFPPLFRAVVSSGESSGRLGLVLDQLAHHLERSYRLRTLVRSALIYPAILGIMATLM
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTALMVWVVPKLVEQFAMLGADELPPLTRFVIGLSGFVRDWGLFVLVGGAICLYLSTIVL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAPAWKLRWDAFVLRIPFFGDMARKVSAARFARIHATMSGAGATVLESLSGARNAMGNMV
HCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FRSAADQILEIVQRGGALSSAMKSTGVFPQMMVHMVASGEAARNVPAMMNRAADFLEDEF
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
ETATNVALGLLEPMIIIFLGGIVGTIVLSIMLPIMQLNTLALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]