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The map label for this gene is rlmB [H]

Identifier: 114797823

GI number: 114797823

Start: 2846253

End: 2847062

Strand: Direct

Name: rlmB [H]

Synonym: HNE_2686

Alternate gene names: 114797823

Gene position: 2846253-2847062 (Clockwise)

Preceding gene: 114798644

Following gene: 114798219

Centisome position: 76.82

GC content: 66.91

Gene sequence:

>810_bases
ATGGCGCGCAACCACCCAAAGCGGCCCCCGAACACCGATGTGGCAACACGCCCCGGCCGCCCCGAAAACCAGCGCTTTTC
GCGCCGCGATGAGGAAGGCGGCCCCGTCTGGCTGTGGGGAACCCATGCCGTAATGGCAGCCCTGGCCAACCCGGCCCGCA
AATTCCACACCCTGCTGGCGACCGAAAATGCCGCCCAGAAGCTGCCCGCTGGCGTGATCTCGCCCCAACTGGCAACCGCC
AAGGAGATCGACAACCGCCTGCCCCCCGGCGCGGTCCACCAGGGCATTGCCCTGCGGGCAGACCCGCTGGAAGAAGCAAT
TCTGGAAGATGTCATCGCCGCAGGCGCCACGCGAATCGCCGTGCTTGATCAGGTGTCTGACCCCCACAACCTCGGCGCCA
TCTTCCGCTCCGCCGCCGCGTTCGGCTTTGAGGGGCTCGTGCTGCAGACGCGCAACGCCCCGCCGATCACCGGAATTGTC
GCCAAAAGTGCGGCCGGCGCCATCGAAACGGTCACCGAGATCCGCGCGGTAAACATCGCCCGCGCGCTCGAACAGCTCGC
CGAGGCTGATTTCCACACCGTTGGCCTCGCCGGCGAAAGCACGCGGTCTCTGGATGAGGCCGTCAAAGGCGCAGCGAAAG
TCGCCATCGTCCTGGGCGCCGAAGGCTCTGGCCTGCGCCCGGCCGTGGCCAAGGCCTGCTCCGAACTCGCCCGCATCCCG
ATCGCCGCGCAAATGGAAAGCCTCAACGTGTCCAATGCTGCCGCAATTGCGTTTTATGAGGCGAGCCGGGGCAGTTTCCC
TGCCCGCTGA

Upstream 100 bases:

>100_bases
CGCGAACACACGCCGTTTGACGCCGCCGCGCAGTCGCTTCACAAGGGCAGTTTACCGGCCCCGGCTCCACGCTGGAAGCG
GCCTTATAAGGACGAACATC

Downstream 100 bases:

>100_bases
CTCTGTTACACTGCTCCACACTACGGGCCGGGAGACGCGAGCATGATGCGCTTTATTTCAATCCTTCTGGTGATCGCAGG
TCTCCTTGCAGCCGGCATCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MARNHPKRPPNTDVATRPGRPENQRFSRRDEEGGPVWLWGTHAVMAALANPARKFHTLLATENAAQKLPAGVISPQLATA
KEIDNRLPPGAVHQGIALRADPLEEAILEDVIAAGATRIAVLDQVSDPHNLGAIFRSAAAFGFEGLVLQTRNAPPITGIV
AKSAAGAIETVTEIRAVNIARALEQLAEADFHTVGLAGESTRSLDEAVKGAAKVAIVLGAEGSGLRPAVAKACSELARIP
IAAQMESLNVSNAAAIAFYEASRGSFPAR

Sequences:

>Translated_269_residues
MARNHPKRPPNTDVATRPGRPENQRFSRRDEEGGPVWLWGTHAVMAALANPARKFHTLLATENAAQKLPAGVISPQLATA
KEIDNRLPPGAVHQGIALRADPLEEAILEDVIAAGATRIAVLDQVSDPHNLGAIFRSAAAFGFEGLVLQTRNAPPITGIV
AKSAAGAIETVTEIRAVNIARALEQLAEADFHTVGLAGESTRSLDEAVKGAAKVAIVLGAEGSGLRPAVAKACSELARIP
IAAQMESLNVSNAAAIAFYEASRGSFPAR
>Mature_268_residues
ARNHPKRPPNTDVATRPGRPENQRFSRRDEEGGPVWLWGTHAVMAALANPARKFHTLLATENAAQKLPAGVISPQLATAK
EIDNRLPPGAVHQGIALRADPLEEAILEDVIAAGATRIAVLDQVSDPHNLGAIFRSAAAFGFEGLVLQTRNAPPITGIVA
KSAAGAIETVTEIRAVNIARALEQLAEADFHTVGLAGESTRSLDEAVKGAAKVAIVLGAEGSGLRPAVAKACSELARIPI
AAQMESLNVSNAAAIAFYEASRGSFPAR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=197, Percent_Identity=30.9644670050761, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=35.9832635983264, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1788935, Length=146, Percent_Identity=32.1917808219178, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=251, Percent_Identity=31.8725099601594, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28156; Mature: 28025

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNHPKRPPNTDVATRPGRPENQRFSRRDEEGGPVWLWGTHAVMAALANPARKFHTLLA
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
TENAAQKLPAGVISPQLATAKEIDNRLPPGAVHQGIALRADPLEEAILEDVIAAGATRIA
CCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCCEECCCHHHHHHHHHHHHCCCHHEE
VLDQVSDPHNLGAIFRSAAAFGFEGLVLQTRNAPPITGIVAKSAAGAIETVTEIRAVNIA
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHH
RALEQLAEADFHTVGLAGESTRSLDEAVKGAAKVAIVLGAEGSGLRPAVAKACSELARIP
HHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCC
IAAQMESLNVSNAAAIAFYEASRGSFPAR
HHHHHHHCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
ARNHPKRPPNTDVATRPGRPENQRFSRRDEEGGPVWLWGTHAVMAALANPARKFHTLLA
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
TENAAQKLPAGVISPQLATAKEIDNRLPPGAVHQGIALRADPLEEAILEDVIAAGATRIA
CCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCCEECCCHHHHHHHHHHHHCCCHHEE
VLDQVSDPHNLGAIFRSAAAFGFEGLVLQTRNAPPITGIVAKSAAGAIETVTEIRAVNIA
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHH
RALEQLAEADFHTVGLAGESTRSLDEAVKGAAKVAIVLGAEGSGLRPAVAKACSELARIP
HHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCC
IAAQMESLNVSNAAAIAFYEASRGSFPAR
HHHHHHHCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]